P38346 (BIT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable target of rapamycin complex 2 subunit BIT2
Short name=TORC2 subunit BIT2

Alternative name(s):
Binding partner of TOR2 protein 2

Gene names

Name:	BIT2
Ordered Locus Names:	YBR270C
ORF Names:	YBR1738

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure

Interacts with the target of rapamycin complex 2 (TORC2) subunit TSC11 and the TORC2 effectors SLM1 and SLM2. Ref.3 Ref.4

Ontologies

Keywords
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	protein binding Inferred from physical interaction Ref.3. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GCN3	P14741	3	EBI-21144,EBI-6253

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 545	545	Probable target of rapamycin complex 2 subunit BIT2		PRO_0000202530
Experimental info
	Sequence conflict	152 – 153	2	SG → RA in CAA85233. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P38346 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 84215A8C09614321	FASTA	545	61,219
	10 20 30 40 50 60 MATDLNRKRS ATSGSLSVTN PNIKATNRKP ARVYSVSSDI VPQALTHPDE DVHLKTSKSP 70 80 90 100 110 120 HDAAPRWSQV GFQSIFHDGS NARRSTDSIE EEYSQGTENN DGHSEIGSSS SNRMEGNTTS 130 140 150 160 170 180 NDSLFSSNSR GNKRRLSIFT NSKDNMRNRS RSGSKNYGTV ITGTSSNNIS RSGSKLFHTK 190 200 210 220 230 240 SNMSVNSLQS SLSTGHSHSN KGSNVFSKMA KKLLPYKPHN SIGKDDVEPV VPSPFSKFLH 250 260 270 280 290 300 SSYGKHRSPV QFIHTSTGGL IDSGKSVYSF NPSINNNPND TALSLIQDDA FDATNVSLLH 310 320 330 340 350 360 DLLKNLPSLI ANYKSFTVQE LFVLEGNIWG IYCSIVVELF KNKRVWQLPA KIEDIDRLLE 370 380 390 400 410 420 FYITLKTQTK AAVTHSRFLA EIEEFITTSL YILENQIVFN YANEDTVNTA LKRVGIIWKV 430 440 450 460 470 480 FYQQVYYDMM AVLLPFEKSF QKNSNYWLDG YLSEPSRYAP SIDVLLLKCF RDSIILPYYE 490 500 510 520 530 540 SFLHTNDGAS KSFQRYIFSE EEQNGVTEED KLTLLQCFGI LNTIKGNSRN QRIIGELLEG IRMSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 152-152. Strain: ATCC 204508 / S288c.
[3]	"A comprehensive two-hybrid analysis to explore the yeast protein interactome." Ito T., Chiba T., Ozawa R., Yoshida M., Hattori M., Sakaki Y. Proc. Natl. Acad. Sci. U.S.A. 98:4569-4574(2001) [PubMed: 11283351] [Abstract] Cited for: INTERACTION WITH SLM1; SLM2 AND TSC11.
[4]	"The pleckstrin homology domain proteins Slm1 and Slm2 are required for actin cytoskeleton organization in yeast and bind phosphatidylinositol-4,5-bisphosphate and TORC2." Fadri M., Daquinag A., Wang S., Xue T., Kunz J. Mol. Biol. Cell 16:1883-1900(2005) [PubMed: 15689497] [Abstract] Cited for: INTERACTION WITH SLM1 AND SLM2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z36139 Genomic DNA. Translation: CAA85233.1. BK006936 Genomic DNA. Translation: DAA07386.2.
PIR	S46151.
RefSeq	NP_009829.2. NM_001178618.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1443N.
IntAct	P38346. 13 interactions.
MINT	MINT-385545.
STRING	P38346.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	852573.
KEGG	sce:YBR270C.
NMPDR	fig|4932.3.peg.545.
Organism-specific databases
CYGD	YBR270c.
SGD	S000000474. BIT2.
Phylogenomic databases
eggNOG	fuNOG08459.
GeneTree	EFGT00050000008665.
HOGENOM	HBG204224.
OMA	WQLPAKI.
OrthoDB	EOG4JHGQK.
Gene expression databases
ArrayExpress	P38346.
Genevestigator	P38346.
GermOnline	YBR270C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013745. HbrB. [Graphical view]
Pfam	PF08539. HbrB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971703.

Entry information

Entry name

BIT2_YEAST

Accession

Primary (citable) accession number: P38346
Secondary accession number(s): D6VQR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	July 27, 2011
Last modified:	January 25, 2012
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program