Reviewed, UniProtKB/Swiss-Prot P38346 (BIT2_YEAST)

Names and origin

Protein names

Recommended name:
    Probable target of rapamycin complex 2 subunit BIT2
      Short name=TORC2 subunit BIT2
Alternative name(s):
    Binding partner of TOR2 protein 2

Gene names

Name:	BIT2
Ordered Locus Names:	YBR270C
ORF Names:	YBR1738

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure

Interacts with the target of rapamycin complex 2 (TORC2) subunit TSC11 and the TORC2 effectors SLM1 and SLM2. Ref.2 Ref.3

Ontologies

Keywords
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	protein binding Ref.2 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATG17	Q06410	1	EBI-21144,EBI-30856
BBP1	Q12365	1	EBI-21144,EBI-3448
GCN3	P14741	1	EBI-21144,EBI-6253
NIP29	P33419	1	EBI-21144,EBI-12041
SLM1	P40485	1	EBI-21144,EBI-25172
SLM2	P53955	1	EBI-21144,EBI-28706
TSC11	P40061	1	EBI-21144,EBI-22621
YAP6	Q03935	1	EBI-21144,EBI-37603

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 545	545	Probable target of rapamycin complex 2 subunit BIT2		PRO_0000202530

Sequences

	Sequence		Length	Mass (Da)
	P38346-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 5655D51206EF728B	FASTA	545	61,302
	10 20 30 40 50 60 MATDLNRKRS ATSGSLSVTN PNIKATNRKP ARVYSVSSDI VPQALTHPDE DVHLKTSKSP 70 80 90 100 110 120 HDAAPRWSQV GFQSIFHDGS NARRSTDSIE EEYSQGTENN DGHSEIGSSS SNRMEGNTTS 130 140 150 160 170 180 NDSLFSSNSR GNKRRLSIFT NSKDNMRNRS RRASKNYGTV ITGTSSNNIS RSGSKLFHTK 190 200 210 220 230 240 SNMSVNSLQS SLSTGHSHSN KGSNVFSKMA KKLLPYKPHN SIGKDDVEPV VPSPFSKFLH 250 260 270 280 290 300 SSYGKHRSPV QFIHTSTGGL IDSGKSVYSF NPSINNNPND TALSLIQDDA FDATNVSLLH 310 320 330 340 350 360 DLLKNLPSLI ANYKSFTVQE LFVLEGNIWG IYCSIVVELF KNKRVWQLPA KIEDIDRLLE 370 380 390 400 410 420 FYITLKTQTK AAVTHSRFLA EIEEFITTSL YILENQIVFN YANEDTVNTA LKRVGIIWKV 430 440 450 460 470 480 FYQQVYYDMM AVLLPFEKSF QKNSNYWLDG YLSEPSRYAP SIDVLLLKCF RDSIILPYYE 490 500 510 520 530 540 SFLHTNDGAS KSFQRYIFSE EEQNGVTEED KLTLLQCFGI LNTIKGNSRN QRIIGELLEG IRMSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"A comprehensive two-hybrid analysis to explore the yeast protein interactome." Ito T., Chiba T., Ozawa R., Yoshida M., Hattori M., Sakaki Y. Proc. Natl. Acad. Sci. U.S.A. 98:4569-4574(2001) [PubMed: 11283351] [Abstract] Cited for: INTERACTION WITH SLM1; SLM2 AND TSC11.
[3]	"The pleckstrin homology domain proteins Slm1 and Slm2 are required for actin cytoskeleton organization in yeast and bind phosphatidylinositol-4,5-bisphosphate and TORC2." Fadri M., Daquinag A., Wang S., Xue T., Kunz J. Mol. Biol. Cell 16:1883-1900(2005) [PubMed: 15689497] [Abstract] Cited for: INTERACTION WITH SLM1 AND SLM2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z36139 Genomic DNA. Translation: CAA85233.1.
PIR	S46151.
RefSeq	NP_009829.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1443N.
IntAct	P38346. 21 interactions.
STRING	P38346.
Genome annotation databases
Ensembl	YBR270C; YBR270C; YBR270C; Saccharomyces cerevisiae. [Genome view]
GeneID	852573.
KEGG	sce:YBR270C.
NMPDR	fig|4932.3.peg.545.
Organism-specific databases
CYGD	YBR270c.
SGD	S000000474. BIT2.
Phylogenomic databases
HOGENOM	HBG204224.
OMA	WQLPAKI.
OrthoDB	EOG9ZGQVZ.
Gene expression databases
ArrayExpress	P38346.
Genevestigator	P38346.
GermOnline	YBR270C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013745. HbrB. [Graphical view]
Pfam	PF08539. HbrB. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	971703.

Entry information

Entry name

BIT2_YEAST

Accession

Primary (citable) accession number: P38346

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 15, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)