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Protein

Cytoplasmic 60S subunit biogenesis factor REI1

Gene

REI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pre-60S-associated factor involved in the cytoplasmic maturation of the 60S subunit. Involved in the dissociation and recycling of other late pre-60S factors like ARX1, TIF6 and ALB1 before newly synthesized large ribosomal subunits enter translation. Cooperates with the co-chaperone JJJ1 in the release of the nuclear-export factor ARX1. May act redundantly with REH1 to directly promote a stabilizing structural rearrangement in cytoplasmic 60S subunit maturation independent on ARX1 recycling.7 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri7 – 3125C2H2-type 1Add
BLAST
Zinc fingeri162 – 18726C2H2-type 2Add
BLAST
Zinc fingeri215 – 23925C2H2-type 3Add
BLAST

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • budding cell bud growth Source: SGD
  • mitotic cell cycle Source: SGD
  • nucleocytoplasmic transport Source: SGD
  • ribosomal large subunit biogenesis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29188-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic 60S subunit biogenesis factor REI1
Alternative name(s):
Required for isotropic bud growth protein 1
pre-60S factor REI1
Gene namesi
Name:REI1
Ordered Locus Names:YBR267W
ORF Names:YBR1736
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR267W.
SGDiS000000471. REI1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393Cytoplasmic 60S subunit biogenesis factor REI1PRO_0000202529Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei140 – 1401PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38344.
PeptideAtlasiP38344.

2D gel databases

SWISS-2DPAGEP38344.

PTM databases

iPTMnetiP38344.

Interactioni

Subunit structurei

Associates with nascent pre-60S particles that have not yet entered the translating pool, and is released from mature 60S subunits. Interacts with pre-60S factors ARX1 and RPL24. Interacts with JJJ1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARX1Q038626EBI-21136,EBI-31385
JJJ1P538636EBI-21136,EBI-29183
LSG1P531457EBI-21136,EBI-23885

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi32962. 219 interactions.
DIPiDIP-6347N.
IntActiP38344. 12 interactions.
MINTiMINT-614813.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5APNelectron microscopy3.91y1-393[»]
ProteinModelPortaliP38344.
SMRiP38344. Positions 5-41, 215-241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the REI1 family.Curated
Contains 3 C2H2-type zinc fingers.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri7 – 3125C2H2-type 1Add
BLAST
Zinc fingeri162 – 18726C2H2-type 2Add
BLAST
Zinc fingeri215 – 23925C2H2-type 3Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000018047.
HOGENOMiHOG000183078.
InParanoidiP38344.
KOiK14816.
OMAiLYCKACR.
OrthoDBiEOG7647F9.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR003604. Znf_U1.
[Graphical view]
PfamiPF12756. zf-C2H2_2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
SM00451. ZnF_U1. 1 hit.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38344-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSGVYTCN SCVLTFDSSD EQRAHMKSDW HRYNLKRRVA QLPPISFETF
60 70 80 90 100
DSKVSAAAAS TSKSAEKEKP VTKKELKRRE KQALLEKKKK LLEIARANML
110 120 130 140 150
ENMQKSQEGN TPDLSKLSLQ ENEENKEKEE PKKEEPEQLT EEEMAERVMQ
160 170 180 190 200
EKLRNRVDIP LEQCLFCEHN KHFKDVEENL EHMFRTHGFY IPEQKYLVDK
210 220 230 240 250
IGLVKYMSEK IGLGNICIVC NYQGRTLTAV RQHMLAKRHC KIPYESEDER
260 270 280 290 300
LEISEFYDFT SSYANFNSNT TPDNEDDWED VGSDEAGSDD EDLPQEYLYN
310 320 330 340 350
DGIELHLPTG IKVGHRSLQR YYKQDLKPEV ILTEGQGTLV AAETRSFLPA
360 370 380 390
FDKKGVQTQQ RVWQTERFDK KRLDKRSAKF VNNQPHYRDQ LLQ
Length:393
Mass (Da):45,862
Last modified:July 27, 2011 - v3
Checksum:iD01AA8FFDF4A0BAC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 1532KL → NV in CAA49931 (PubMed:8465606).Curated
Sequence conflicti152 – 1532KL → NV in CAA85229 (PubMed:7813418).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70529 Genomic DNA. Translation: CAA49931.1.
Z36135 Genomic DNA. Translation: CAA85229.1.
BK006936 Genomic DNA. Translation: DAA07383.2.
PIRiS77691.
RefSeqiNP_009825.4. NM_001178615.4.

Genome annotation databases

EnsemblFungiiYBR267W; YBR267W; YBR267W.
GeneIDi852569.
KEGGisce:YBR267W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70529 Genomic DNA. Translation: CAA49931.1.
Z36135 Genomic DNA. Translation: CAA85229.1.
BK006936 Genomic DNA. Translation: DAA07383.2.
PIRiS77691.
RefSeqiNP_009825.4. NM_001178615.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5APNelectron microscopy3.91y1-393[»]
ProteinModelPortaliP38344.
SMRiP38344. Positions 5-41, 215-241.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32962. 219 interactions.
DIPiDIP-6347N.
IntActiP38344. 12 interactions.
MINTiMINT-614813.

PTM databases

iPTMnetiP38344.

2D gel databases

SWISS-2DPAGEP38344.

Proteomic databases

MaxQBiP38344.
PeptideAtlasiP38344.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR267W; YBR267W; YBR267W.
GeneIDi852569.
KEGGisce:YBR267W.

Organism-specific databases

EuPathDBiFungiDB:YBR267W.
SGDiS000000471. REI1.

Phylogenomic databases

GeneTreeiENSGT00390000018047.
HOGENOMiHOG000183078.
InParanoidiP38344.
KOiK14816.
OMAiLYCKACR.
OrthoDBiEOG7647F9.

Enzyme and pathway databases

BioCyciYEAST:G3O-29188-MONOMER.

Miscellaneous databases

NextBioi971694.
PROiP38344.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR003604. Znf_U1.
[Graphical view]
PfamiPF12756. zf-C2H2_2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
SM00451. ZnF_U1. 1 hit.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete sequence of a 19,482 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
    Doignon F., Biteau N., Crouzet M., Aigle M.
    Yeast 9:189-199(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Aigle M., Baclet M.C., Barthe C., Biteau N., Crouzet M., Doignon F.
    Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO N-TERMINUS.
  4. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 152-153.
    Strain: ATCC 204508 / S288c.
  5. "Variations of the C2H2 zinc finger motif in the yeast genome and classification of yeast zinc finger proteins."
    Boehm S., Frishman D., Mewes H.-W.
    Nucleic Acids Res. 25:2464-2469(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Ybr267w is a new cytoplasmic protein belonging to the mitotic signaling network of Saccharomyces cerevisiae."
    Iwase M., Toh-e A.
    Cell Struct. Funct. 29:1-15(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  9. "A functional network involved in the recycling of nucleocytoplasmic pre-60S factors."
    Lebreton A., Saveanu C., Decourty L., Rain J.-C., Jacquier A., Fromont-Racine M.
    J. Cell Biol. 173:349-360(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RPL24, ASSOCIATION WITH PRE-60S PARTICLES.
  10. "Nuclear recycling of the pre-60S ribosomal subunit-associated factor Arx1 depends on Rei1 in Saccharomyces cerevisiae."
    Hung N.J., Johnson A.W.
    Mol. Cell. Biol. 26:3718-3727(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ARX1, ASSOCIATION WITH PRE-60S PARTICLES.
  11. "The Hsp40 chaperone Jjj1 is required for the nucleo-cytoplasmic recycling of preribosomal factors in Saccharomyces cerevisiae."
    Demoinet E., Jacquier A., Lutfalla G., Fromont-Racine M.
    RNA 13:1570-1581(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-140, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Functional redundancy of yeast proteins Reh1 and Rei1 in cytoplasmic 60S subunit maturation."
    Parnell K.M., Bass B.L.
    Mol. Cell. Biol. 29:4014-4023(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ASSOCIATION WITH PRE-60S PARTICLES.
  14. "The cytosolic J-protein, Jjj1, and Rei1 function in the removal of the pre-60 S subunit factor Arx1."
    Meyer A.E., Hoover L.A., Craig E.A.
    J. Biol. Chem. 285:961-968(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH JJJ1.
  15. "Cryo-EM structures of Arx1 and maturation factors Rei1 and Jjj1 bound to the 60S ribosomal subunit."
    Greber B.J., Boehringer D., Montellese C., Ban N.
    Nat. Struct. Mol. Biol. 19:1228-1233(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY, FUNCTION, INTERACTION WITH ARX1.

Entry informationi

Entry nameiREI1_YEAST
AccessioniPrimary (citable) accession number: P38344
Secondary accession number(s): D6VQR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 830 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.