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Protein

Cytoplasmic 60S subunit biogenesis factor REI1

Gene

REI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pre-60S-associated factor involved in the cytoplasmic maturation of the 60S subunit. Involved in the dissociation and recycling of other late pre-60S factors like ARX1, TIF6 and ALB1 before newly synthesized large ribosomal subunits enter translation. Cooperates with the co-chaperone JJJ1 in the release of the nuclear-export factor ARX1. May act redundantly with REH1 to directly promote a stabilizing structural rearrangement in cytoplasmic 60S subunit maturation independent on ARX1 recycling.7 Publications

Miscellaneous

Present with 830 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri7 – 31C2H2-type 1Add BLAST25
Zinc fingeri162 – 187C2H2-type 2Add BLAST26
Zinc fingeri215 – 239C2H2-type 3Add BLAST25

GO - Molecular functioni

GO - Biological processi

  • budding cell bud growth Source: SGD
  • mitotic cell cycle Source: SGD
  • nucleocytoplasmic transport Source: SGD
  • ribosomal large subunit biogenesis Source: SGD

Keywordsi

Biological processRibosome biogenesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29188-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic 60S subunit biogenesis factor REI1
Alternative name(s):
Required for isotropic bud growth protein 1
pre-60S factor REI1
Gene namesi
Name:REI1
Ordered Locus Names:YBR267W
ORF Names:YBR1736
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR267W.
SGDiS000000471. REI1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • preribosome, large subunit precursor Source: SGD

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002025291 – 393Cytoplasmic 60S subunit biogenesis factor REI1Add BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei140PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38344.
PRIDEiP38344.

2D gel databases

SWISS-2DPAGEiP38344.

PTM databases

iPTMnetiP38344.

Interactioni

Subunit structurei

Associates with nascent pre-60S particles that have not yet entered the translating pool, and is released from mature 60S subunits. Interacts with pre-60S factors ARX1 and RPL24. Interacts with JJJ1.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32962. 432 interactors.
DIPiDIP-6347N.
IntActiP38344. 12 interactors.
MINTiMINT-614813.
STRINGi4932.YBR267W.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5APNelectron microscopy3.91y1-393[»]
ProteinModelPortaliP38344.
SMRiP38344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the REI1 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri7 – 31C2H2-type 1Add BLAST25
Zinc fingeri162 – 187C2H2-type 2Add BLAST26
Zinc fingeri215 – 239C2H2-type 3Add BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000018047.
HOGENOMiHOG000183078.
InParanoidiP38344.
KOiK14816.
OMAiVDKDAMN.
OrthoDBiEOG092C2EXB.

Family and domain databases

InterProiView protein in InterPro
IPR003604. Matrin/U1-like-C_Znf_C2H2.
IPR013087. Znf_C2H2_type.
SMARTiView protein in SMART
SM00355. ZnF_C2H2. 3 hits.
SM00451. ZnF_U1. 1 hit.
SUPFAMiSSF57667. SSF57667. 1 hit.
PROSITEiView protein in PROSITE
PS00028. ZINC_FINGER_C2H2_1. 1 hit.

Sequencei

Sequence statusi: Complete.

P38344-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSGVYTCN SCVLTFDSSD EQRAHMKSDW HRYNLKRRVA QLPPISFETF
60 70 80 90 100
DSKVSAAAAS TSKSAEKEKP VTKKELKRRE KQALLEKKKK LLEIARANML
110 120 130 140 150
ENMQKSQEGN TPDLSKLSLQ ENEENKEKEE PKKEEPEQLT EEEMAERVMQ
160 170 180 190 200
EKLRNRVDIP LEQCLFCEHN KHFKDVEENL EHMFRTHGFY IPEQKYLVDK
210 220 230 240 250
IGLVKYMSEK IGLGNICIVC NYQGRTLTAV RQHMLAKRHC KIPYESEDER
260 270 280 290 300
LEISEFYDFT SSYANFNSNT TPDNEDDWED VGSDEAGSDD EDLPQEYLYN
310 320 330 340 350
DGIELHLPTG IKVGHRSLQR YYKQDLKPEV ILTEGQGTLV AAETRSFLPA
360 370 380 390
FDKKGVQTQQ RVWQTERFDK KRLDKRSAKF VNNQPHYRDQ LLQ
Length:393
Mass (Da):45,862
Last modified:July 27, 2011 - v3
Checksum:iD01AA8FFDF4A0BAC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti152 – 153KL → NV in CAA49931 (PubMed:8465606).Curated2
Sequence conflicti152 – 153KL → NV in CAA85229 (PubMed:7813418).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70529 Genomic DNA. Translation: CAA49931.1.
Z36135 Genomic DNA. Translation: CAA85229.1.
BK006936 Genomic DNA. Translation: DAA07383.2.
PIRiS77691.
RefSeqiNP_009825.4. NM_001178615.4.

Genome annotation databases

EnsemblFungiiYBR267W; YBR267W; YBR267W.
GeneIDi852569.
KEGGisce:YBR267W.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiREI1_YEAST
AccessioniPrimary (citable) accession number: P38344
Secondary accession number(s): D6VQR3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 27, 2011
Last modified: July 5, 2017
This is version 137 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names