Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-ketodihydrosphingosine reductase TSC10

Gene

TSC10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of 3-ketodihydrosphingosine (KDS) to dihydrosphingosine (DHS).1 Publication

Catalytic activityi

Sphinganine + NADP+ = 3-dehydrosphinganine + NADPH.

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei167 – 1671SubstrateBy similarity
Active sitei180 – 1801Proton acceptorBy similarity
Active sitei184 – 1841By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 3727NADPBy similarityAdd
BLAST

GO - Molecular functioni

  • 3-dehydrosphinganine reductase activity Source: SGD

GO - Biological processi

  • 3-keto-sphinganine metabolic process Source: SGD
  • sphingolipid biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:YBR265W-MONOMER.
YEAST:YBR265W-MONOMER.
UniPathwayiUPA00222.

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketodihydrosphingosine reductase TSC10 (EC:1.1.1.102)
Alternative name(s):
3-dehydrosphinganine reductase
KDS reductase
Temperature-sensitive CSG2 suppressor protein 10
Gene namesi
Name:TSC10
Ordered Locus Names:YBR265W
ORF Names:YBR1734
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR265W.
SGDiS000000469. TSC10.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei280 – 30021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • lipid particle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3203203-ketodihydrosphingosine reductase TSC10PRO_0000054800Add
BLAST

Proteomic databases

MaxQBiP38342.
PeptideAtlasiP38342.

Interactioni

Protein-protein interaction databases

BioGridi32961. 68 interactions.
DIPiDIP-4781N.
IntActiP38342. 4 interactions.
MINTiMINT-488282.

Structurei

3D structure databases

ProteinModelPortaliP38342.
SMRiP38342. Positions 7-209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000248715.
InParanoidiP38342.
KOiK04708.
OMAiPLANWIV.
OrthoDBiEOG7R2BWK.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PfamiPF00106. adh_short. 2 hits.
[Graphical view]
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 2 hits.

Sequencei

Sequence statusi: Complete.

P38342-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFTLEDQVV LITGGSQGLG KEFAKKYYNE AENTKIIIVS RSEARLLDTC
60 70 80 90 100
NEIRIEAHLR RETTDEGQVQ HKLAAPLDLE QRLFYYPCDL SCYESVECLF
110 120 130 140 150
NALRDLDLLP TQTLCCAGGA VPKLFRGLSG HELNLGMDIN YKTTLNVAHQ
160 170 180 190 200
IALAEQTKEH HLIIFSSATA LYPFVGYSQY APAKAAIKSL VAILRQELTN
210 220 230 240 250
FRISCVYPGN FESEGFTVEQ LTKPEITKLI EGPSDAIPCK QACDIIAKSL
260 270 280 290 300
ARGDDDVFTD FVGWMIMGMD LGLTAKKSRF VPLQWIFGVL SNILVVPFYM
310 320
VGCSWYIRKW FRENDGKKAN
Length:320
Mass (Da):35,973
Last modified:July 27, 2011 - v2
Checksum:iC5F251B4099E6242
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti255 – 2551D → E in CAA49930 (PubMed:8465606).Curated
Sequence conflicti255 – 2551D → E in CAA85228 (PubMed:7813418).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70529 Genomic DNA. Translation: CAA49930.1.
Z36134 Genomic DNA. Translation: CAA85228.1.
BK006936 Genomic DNA. Translation: DAA07382.2.
PIRiS32966.
RefSeqiNP_009824.2. NM_001178613.2.

Genome annotation databases

EnsemblFungiiYBR265W; YBR265W; YBR265W.
GeneIDi852568.
KEGGisce:YBR265W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70529 Genomic DNA. Translation: CAA49930.1.
Z36134 Genomic DNA. Translation: CAA85228.1.
BK006936 Genomic DNA. Translation: DAA07382.2.
PIRiS32966.
RefSeqiNP_009824.2. NM_001178613.2.

3D structure databases

ProteinModelPortaliP38342.
SMRiP38342. Positions 7-209.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32961. 68 interactions.
DIPiDIP-4781N.
IntActiP38342. 4 interactions.
MINTiMINT-488282.

Proteomic databases

MaxQBiP38342.
PeptideAtlasiP38342.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR265W; YBR265W; YBR265W.
GeneIDi852568.
KEGGisce:YBR265W.

Organism-specific databases

EuPathDBiFungiDB:YBR265W.
SGDiS000000469. TSC10.

Phylogenomic databases

HOGENOMiHOG000248715.
InParanoidiP38342.
KOiK04708.
OMAiPLANWIV.
OrthoDBiEOG7R2BWK.

Enzyme and pathway databases

UniPathwayiUPA00222.
BioCyciMetaCyc:YBR265W-MONOMER.
YEAST:YBR265W-MONOMER.

Miscellaneous databases

NextBioi971691.
PROiP38342.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PfamiPF00106. adh_short. 2 hits.
[Graphical view]
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete sequence of a 19,482 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
    Doignon F., Biteau N., Crouzet M., Aigle M.
    Yeast 9:189-199(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 255.
    Strain: ATCC 204508 / S288c.
  4. "The Saccharomyces cerevisiae TSC10/YBR265w gene encoding 3-ketosphinganine reductase is identified in a screen for temperature-sensitive suppressors of the Ca2+-sensitive csg2Delta mutant."
    Beeler T., Bacikova D., Gable K., Hopkins L., Johnson C., Slife H., Dunn T.
    J. Biol. Chem. 273:30688-30694(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTSC10_YEAST
AccessioniPrimary (citable) accession number: P38342
Secondary accession number(s): D6VQR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.