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Protein

Alpha N-terminal protein methyltransferase 1

Gene

TAE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Responsible for the N-terminal methylation of the ribosomal proteins RPL12A, RPL12B, RPS25A and RPS25B.1 Publication

Miscellaneous

Present with 3060 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein].
2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein].

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei76S-adenosyl-L-methionineBy similarity1
Binding sitei139S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • N-terminal protein N-methyltransferase activity Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • N-terminal peptidyl-proline dimethylation Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-29185-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha N-terminal protein methyltransferase 1 (EC:2.1.1.244)
Alternative name(s):
Translation associated element 1
X-Pro-Lys N-terminal protein methyltransferase 1
Short name:
NTM1
Gene namesi
Name:TAE1
Synonyms:NTM1
Ordered Locus Names:YBR261C
ORF Names:YBR1729
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR261C.
SGDiS000000465. TAE1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Defects in both translation efficiency and fidelity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192921 – 232Alpha N-terminal protein methyltransferase 1Add BLAST232

Proteomic databases

MaxQBiP38340.
PRIDEiP38340.

PTM databases

iPTMnetiP38340.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ABI3Q9P2A43EBI-21116,EBI-742038From Homo sapiens.
ACTBP607093EBI-21116,EBI-353944From Homo sapiens.
ACTG1P632613EBI-21116,EBI-351292From Homo sapiens.
AGTRAPQ6RW13-23EBI-21116,EBI-11522760From a different organism.
AIMP2Q131553EBI-21116,EBI-745226From Homo sapiens.
BLMHQ138673EBI-21116,EBI-718504From Homo sapiens.
C1orf50Q9BV193EBI-21116,EBI-2874661From Homo sapiens.
CBSP355203EBI-21116,EBI-740135From Homo sapiens.
CEP44Q9C0F13EBI-21116,EBI-744115From Homo sapiens.
CEP55Q53EZ43EBI-21116,EBI-747776From Homo sapiens.
CEP70Q8NHQ13EBI-21116,EBI-739624From Homo sapiens.
CEP70Q8NHQ1-33EBI-21116,EBI-11526150From a different organism.
CEP76Q8TAP63EBI-21116,EBI-742887From Homo sapiens.
CFL2Q9Y2813EBI-21116,EBI-351218From Homo sapiens.
CMTM5Q96DZ9-23EBI-21116,EBI-11522780From a different organism.
COILP384323EBI-21116,EBI-945751From Homo sapiens.
DAZAP2Q150383EBI-21116,EBI-724310From Homo sapiens.
DMRTB1Q96MA13EBI-21116,EBI-954466From Homo sapiens.
DNAJA3Q96EY1-33EBI-21116,EBI-11526226From a different organism.
DNPEPQ9ULA03EBI-21116,EBI-748356From Homo sapiens.
DTX2Q86UW93EBI-21116,EBI-740376From Homo sapiens.
EFHC2Q5JST63EBI-21116,EBI-2349927From Homo sapiens.
ERVK3-1Q96A103EBI-21116,EBI-10486892From Homo sapiens.
FAM118AQ9NWS63EBI-21116,EBI-8638992From Homo sapiens.
FATE1Q969F03EBI-21116,EBI-743099From Homo sapiens.
FBF1A0A0R4J2E43EBI-21116,EBI-11525602From Homo sapiens.
FSBPO95073-23EBI-21116,EBI-10696047From a different organism.
FTLP027923EBI-21116,EBI-713279From Homo sapiens.
GMDSO605473EBI-21116,EBI-746373From Homo sapiens.
GNMTQ147493EBI-21116,EBI-744239From Homo sapiens.
GSTA4O152173EBI-21116,EBI-752440From Homo sapiens.
HNRNPCP079103EBI-21116,EBI-357966From Homo sapiens.
HNRNPCP07910-23EBI-21116,EBI-5280084From a different organism.
HOMER3Q9NSC53EBI-21116,EBI-748420From Homo sapiens.
HPRT1P004923EBI-21116,EBI-748210From Homo sapiens.
HSD17B14Q9BPX13EBI-21116,EBI-742664From Homo sapiens.
HSPA9P386463EBI-21116,EBI-354932From Homo sapiens.
IMPDH2P122683EBI-21116,EBI-353389From Homo sapiens.
JAKMIP2Q96AA83EBI-21116,EBI-752007From Homo sapiens.
KCTD4Q8WVF53EBI-21116,EBI-741463From Homo sapiens.
KHDRBS2Q5VWX13EBI-21116,EBI-742808From Homo sapiens.
KHDRBS3O755253EBI-21116,EBI-722504From Homo sapiens.
KLHL2O951983EBI-21116,EBI-746999From Homo sapiens.
LDHBP071953EBI-21116,EBI-358748From Homo sapiens.
MAPK1IP1LQ8NDC03EBI-21116,EBI-741424From Homo sapiens.
MX1P205913EBI-21116,EBI-929476From Homo sapiens.
MYD88Q998363EBI-21116,EBI-447677From Homo sapiens.
NECAB2Q7Z6G3-23EBI-21116,EBI-10172876From a different organism.
NFYAP23511-23EBI-21116,EBI-11061759From a different organism.
NME1P155313EBI-21116,EBI-741141From Homo sapiens.
NMNAT1Q9HAN93EBI-21116,EBI-3917542From Homo sapiens.
PHC2Q8IXK0-53EBI-21116,EBI-11527347From a different organism.
PLSCR3Q9NRY63EBI-21116,EBI-750734From Homo sapiens.
PPCDCQ96CD23EBI-21116,EBI-724333From Homo sapiens.
PRPS1P608913EBI-21116,EBI-749195From Homo sapiens.
PSMC6P623333EBI-21116,EBI-357669From Homo sapiens.
PSME3P612893EBI-21116,EBI-355546From Homo sapiens.
PTSQ033933EBI-21116,EBI-712344From Homo sapiens.
PUF60Q9UHX1-23EBI-21116,EBI-11529177From a different organism.
PUF60Q9UHX1-53EBI-21116,EBI-11526420From a different organism.
PUF60Q9UHX1-63EBI-21116,EBI-11085298From a different organism.
PYCR3A0A0A0MQS13EBI-21116,EBI-11528247From Homo sapiens.
QKIQ96PU83EBI-21116,EBI-945792From Homo sapiens.
RABAC1Q9UI143EBI-21116,EBI-712367From Homo sapiens.
RBMXP381593EBI-21116,EBI-743526From Homo sapiens.
RBMY1JQ154153EBI-21116,EBI-8642021From Homo sapiens.
RBPMSQ93062-33EBI-21116,EBI-740343From a different organism.
RCBTB2O951993EBI-21116,EBI-742404From Homo sapiens.
REEP6Q96HR93EBI-21116,EBI-750345From Homo sapiens.
RNF40O751503EBI-21116,EBI-744408From Homo sapiens.
RNF5Q999423EBI-21116,EBI-348482From Homo sapiens.
RPIAP492473EBI-21116,EBI-744831From Homo sapiens.
RTN4Q9NQC3-33EBI-21116,EBI-11526335From a different organism.
SAMD3Q8N6K7-23EBI-21116,EBI-11528848From a different organism.
SF3B4Q154273EBI-21116,EBI-348469From Homo sapiens.
SH3GLB1Q9Y3713EBI-21116,EBI-2623095From Homo sapiens.
SIAH1Q8IUQ4-23EBI-21116,EBI-11522811From a different organism.
SMN2Q166373EBI-21116,EBI-395421From Homo sapiens.
SMN2Q16637-33EBI-21116,EBI-395447From a different organism.
SOD2A0A0C4DFU23EBI-21116,EBI-11528264From Homo sapiens.
SPERTQ8NA61-23EBI-21116,EBI-11524851From a different organism.
SPOPO437913EBI-21116,EBI-743549From Homo sapiens.
SSSCA1O602323EBI-21116,EBI-741415From Homo sapiens.
SSX2IPQ9Y2D83EBI-21116,EBI-2212028From Homo sapiens.
STAC3Q96MF23EBI-21116,EBI-745680From Homo sapiens.
SYCE1Q8N0S23EBI-21116,EBI-6872807From Homo sapiens.
TDO2P487753EBI-21116,EBI-743494From Homo sapiens.
TFCP2Q128003EBI-21116,EBI-717422From Homo sapiens.
THAP1Q9NVV93EBI-21116,EBI-741515From Homo sapiens.
TMEM239Q8WW34-23EBI-21116,EBI-11528917From a different organism.
TNIP1Q150253EBI-21116,EBI-357849From Homo sapiens.
TRAF4Q9BUZ43EBI-21116,EBI-3650647From Homo sapiens.
TRAF5O004633EBI-21116,EBI-523498From Homo sapiens.
TRIM32Q130493EBI-21116,EBI-742790From Homo sapiens.
TRIM38O006353EBI-21116,EBI-2130415From Homo sapiens.
TRIM55Q9BYV6-23EBI-21116,EBI-11522718From a different organism.
TRIM69Q86WT6-23EBI-21116,EBI-11525489From a different organism.
UBE2IP632793EBI-21116,EBI-80168From Homo sapiens.
UBXN11Q5T124-63EBI-21116,EBI-11524408From a different organism.
UGP2E7EUC73EBI-21116,EBI-11528286From Homo sapiens.
UPP2O95045-23EBI-21116,EBI-11528386From a different organism.
XIAPP981703EBI-21116,EBI-517127From Homo sapiens.
ZBED1O960063EBI-21116,EBI-740037From Homo sapiens.
ZBTB26Q9HCK03EBI-21116,EBI-3918996From Homo sapiens.
ZBTB8AQ96BR93EBI-21116,EBI-742740From Homo sapiens.
ZNF212Q9UDV63EBI-21116,EBI-1640204From Homo sapiens.

Protein-protein interaction databases

BioGridi32957. 109 interactors.
DIPiDIP-4951N.
IntActiP38340. 150 interactors.
MINTiMINT-508128.
STRINGi4932.YBR261C.

Structurei

3D structure databases

ProteinModelPortaliP38340.
SMRiP38340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni123 – 124S-adenosyl-L-methionine bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000008371.
InParanoidiP38340.
KOiK16219.
OMAiPVYMIAC.
OrthoDBiEOG092C2UR7.

Family and domain databases

InterProiView protein in InterPro
IPR008576. MeTrfase_NTM1.
IPR029063. SAM-dependent_MTases.
PANTHERiPTHR12753. PTHR12753. 1 hit.
PfamiView protein in Pfam
PF05891. Methyltransf_PK. 1 hit.
PIRSFiPIRSF016958. DUF858_MeTrfase_lik. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

P38340-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVPADSHIK YEDAIDYWTD VDATVDGVLG GYGEGTVVPT MDVLGSNNFL
60 70 80 90 100
RKLKSRMLPQ ENNVKYAVDI GAGIGRVSKT MLHKHAAKID LVEPVKPFIE
110 120 130 140 150
QMHVELAELK DKGQIGQIYE VGMQDWTPDA GKYWLIWCQW CVGHLPDAEL
160 170 180 190 200
VAFLKRCIVG LQPNGTIVVK ENNTPTDTDD FDETDSSVTR SDAKFRQIFE
210 220 230
EAGLKLIASE RQRGLPRELY PVRMYALKPM PN
Length:232
Mass (Da):26,068
Last modified:October 1, 1994 - v1
Checksum:i66699F37B0013088
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70529 Genomic DNA. Translation: CAA49926.1.
Z36130 Genomic DNA. Translation: CAA85224.1.
BK006936 Genomic DNA. Translation: DAA07378.1.
PIRiS32963.
RefSeqiNP_009820.1. NM_001178609.1.

Genome annotation databases

EnsemblFungiiYBR261C; YBR261C; YBR261C.
GeneIDi852564.
KEGGisce:YBR261C.

Similar proteinsi

Entry informationi

Entry nameiNTM1_YEAST
AccessioniPrimary (citable) accession number: P38340
Secondary accession number(s): D6VQQ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 27, 2017
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names