TAE1

Functionⁱ

Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Responsible for the N-terminal methylation of the ribosomal proteins RPL12A, RPL12B, RPS25A and RPS25B.1 Publication

Catalytic activityⁱ

3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein].

2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein].

Sites

Feature key	Position(s)	Length	Description
Binding siteⁱ	71 – 71	1	S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding siteⁱ	76 – 76	1	S-adenosyl-L-methionineBy similarity
Binding siteⁱ	139 – 139	1	S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

GO - Molecular functionⁱ

N-terminal protein N-methyltransferase activity
Source: SGD
Inferred from direct assayⁱ
- 20481588

GO - Biological processⁱ

cytoplasmic translation
Source: SGD
Inferred from mutant phenotypeⁱ
- 19055778
N-terminal peptidyl-proline dimethylation
Source: SGD
Inferred from direct assayⁱ
- 20481588

Complete GO annotation...

Keywords - Molecular functionⁱ

Methyltransferase, Transferase

Keywords - Ligandⁱ

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29185-MONOMER.

BioCycⁱ

YEAST:G3O-29185-MONOMER.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Alpha N-terminal protein methyltransferase 1 (EC:2.1.1.244) Alternative name(s): Translation associated element 1 X-Pro-Lys N-terminal protein methyltransferase 1 Short name: NTM1
Gene namesⁱ	Name:TAE1 Synonyms:NTM1 Ordered Locus Names:YBR261C ORF Names:YBR1729
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome II

Organism-specific databases

EuPathDBⁱ	FungiDB:YBR261C.
SGDⁱ	S000000465. TAE1.

Subcellular locationⁱ

Cytoplasm
1 Publication
Manual assertion based on experiment inⁱ
- Ref.4
  "Global analysis of protein localization in budding yeast."
  Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
  Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
  Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

GO - Cellular componentⁱ

cytosol
Source: SGD
Inferred from direct assayⁱ
- 20481588

Complete GO annotation...

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Defects in both translation efficiency and fidelity.1 Publication

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Chainⁱ	1 – 232	232	Alpha N-terminal protein methyltransferase 1		PRO_0000119292	Add BLAST

Proteomic databases

MaxQBⁱ	P38340.

MaxQBⁱ

P38340.

PTM databases

iPTMnetⁱ	P38340.

iPTMnetⁱ

P38340.

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	32957. 79 interactions.
DIPⁱ	DIP-4951N.
IntActⁱ	P38340. 42 interactions.
MINTⁱ	MINT-508128.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P38340.
SMRⁱ	P38340. Positions 11-228.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Regionⁱ	123 – 124	2	S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesⁱ

Belongs to the methyltransferase superfamily. NTM1 family.Curated

Phylogenomic databases

GeneTreeⁱ	ENSGT00390000008371.
InParanoidⁱ	P38340.
KOⁱ	K16219.
OMAⁱ	ELYPVRM.
OrthoDBⁱ	EOG092C2UR7.

Family and domain databases

Gene3Dⁱ	3.40.50.150. 1 hit.
InterProⁱ	IPR008576. MeTrfase_NTM1. IPR029063. SAM-dependent_MTases. [Graphical view]
PANTHERⁱ	PTHR12753. PTHR12753. 1 hit.
Pfamⁱ	PF05891. Methyltransf_PK. 1 hit. [Graphical view]
PIRSFⁱ	PIRSF016958. DUF858_MeTrfase_lik. 1 hit.
SUPFAMⁱ	SSF53335. SSF53335. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P38340-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDVPADSHIK YEDAIDYWTD VDATVDGVLG GYGEGTVVPT MDVLGSNNFL 
        60         70         80         90        100
RKLKSRMLPQ ENNVKYAVDI GAGIGRVSKT MLHKHAAKID LVEPVKPFIE 
       110        120        130        140        150
QMHVELAELK DKGQIGQIYE VGMQDWTPDA GKYWLIWCQW CVGHLPDAEL 
       160        170        180        190        200
VAFLKRCIVG LQPNGTIVVK ENNTPTDTDD FDETDSSVTR SDAKFRQIFE 
       210        220        230 
EAGLKLIASE RQRGLPRELY PVRMYALKPM PN

Length:232

Mass (Da):26,068

Last modified:October 1, 1994 - v1

Checksum:ⁱ66699F37B0013088

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X70529 Genomic DNA. Translation: CAA49926.1. Z36130 Genomic DNA. Translation: CAA85224.1. BK006936 Genomic DNA. Translation: DAA07378.1.
PIRⁱ	S32963.
RefSeqⁱ	NP_009820.1. NM_001178609.1.

Genome annotation databases

EnsemblFungiⁱ	YBR261C; YBR261C; YBR261C.
GeneIDⁱ	852564.
KEGGⁱ	sce:YBR261C.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X70529 Genomic DNA. Translation: CAA49926.1. Z36130 Genomic DNA. Translation: CAA85224.1. BK006936 Genomic DNA. Translation: DAA07378.1.
PIRⁱ	S32963.
RefSeqⁱ	NP_009820.1. NM_001178609.1.

3D structure databases

ProteinModelPortalⁱ	P38340.
SMRⁱ	P38340. Positions 11-228.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	32957. 79 interactions.
DIPⁱ	DIP-4951N.
IntActⁱ	P38340. 42 interactions.
MINTⁱ	MINT-508128.

PTM databases

iPTMnetⁱ	P38340.

iPTMnetⁱ

P38340.

Proteomic databases

MaxQBⁱ	P38340.

MaxQBⁱ

P38340.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YBR261C; YBR261C; YBR261C.
GeneIDⁱ	852564.
KEGGⁱ	sce:YBR261C.

Organism-specific databases

EuPathDBⁱ	FungiDB:YBR261C.
SGDⁱ	S000000465. TAE1.

Phylogenomic databases

GeneTreeⁱ	ENSGT00390000008371.
InParanoidⁱ	P38340.
KOⁱ	K16219.
OMAⁱ	ELYPVRM.
OrthoDBⁱ	EOG092C2UR7.

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29185-MONOMER.

BioCycⁱ

YEAST:G3O-29185-MONOMER.

Miscellaneous databases

PROⁱ	P38340.

PROⁱ

P38340.

Family and domain databases

Gene3Dⁱ	3.40.50.150. 1 hit.
InterProⁱ	IPR008576. MeTrfase_NTM1. IPR029063. SAM-dependent_MTases. [Graphical view]
PANTHERⁱ	PTHR12753. PTHR12753. 1 hit.
Pfamⁱ	PF05891. Methyltransf_PK. 1 hit. [Graphical view]
PIRSFⁱ	PIRSF016958. DUF858_MeTrfase_lik. 1 hit.
SUPFAMⁱ	SSF53335. SSF53335. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ

NTM1_YEAST

Accessionⁱ

Primary (citable) accession number: P38340
Secondary accession number(s): D6VQQ8

Entry historyⁱ

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	September 7, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry statusⁱ

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Miscellaneousⁱ

Miscellaneous

Present with 3060 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P38340 (NTM1_YEAST)

UniProt

P38340 - NTM1_YEAST

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Alpha N-terminal protein methyltransferase 1

Select a section on the left to see content.

<p>Provides any useful information about the protein, mostly biological knowledge.</p><p><a href='../manual/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>Provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.</p><p><a href='../manual/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>Provides information on the location and the topology of the mature protein in the cell.</p><p><a href='../manual/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>Provides information on the disease(s) and phenotype(s) associated with a protein.</p><p><a href='../manual/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>Describes post-translational modifications (PTMs) and/or processing events.</p><p><a href='../manual/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>Provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.</p><p><a href='../manual/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>Provides information on the tertiary and secondary structure of a protein.</p><p><a href='../manual/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>Provides information on sequence similarities with other proteins and the domain(s) present in a protein.</p><p><a href='../manual/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>Provides information about the sequence similarity with other proteins.</p><p><a href='../manual/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>Displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.</p><p><a href='../manual/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>Is used to point to information related to entries and found in data collections other than UniProtKB.</p><p><a href='../manual/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>Provides general information on the entry.</p><p><a href='../manual/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>Contains any relevant information that doesn’t fit in any other defined sections</p><p><a href='../manual/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Miscellaneous

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ