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P38340

- NTM1_YEAST

UniProt

P38340 - NTM1_YEAST

Protein

Alpha N-terminal protein methyltransferase 1

Gene

TAE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Responsible for the N-terminal methylation of the ribosomal proteins RPL12A, RPL12B, RPS25A and RPS25B.1 Publication

    Catalytic activityi

    3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein].
    2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein].

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei71 – 711S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
    Binding sitei76 – 761S-adenosyl-L-methionineBy similarity
    Binding sitei139 – 1391S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. N-terminal protein N-methyltransferase activity Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. N-terminal peptidyl-proline dimethylation Source: SGD

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29185-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha N-terminal protein methyltransferase 1 (EC:2.1.1.244)
    Alternative name(s):
    Translation associated element 1
    X-Pro-Lys N-terminal protein methyltransferase 1
    Short name:
    NTM1
    Gene namesi
    Name:TAE1
    Synonyms:NTM1
    Ordered Locus Names:YBR261C
    ORF Names:YBR1729
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR261c.
    SGDiS000000465. TAE1.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosol Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Defects in both translation efficiency and fidelity.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 232232Alpha N-terminal protein methyltransferase 1PRO_0000119292Add
    BLAST

    Proteomic databases

    MaxQBiP38340.
    PaxDbiP38340.
    PeptideAtlasiP38340.

    Expressioni

    Gene expression databases

    GenevestigatoriP38340.

    Interactioni

    Protein-protein interaction databases

    BioGridi32957. 79 interactions.
    DIPiDIP-4951N.
    IntActiP38340. 42 interactions.
    MINTiMINT-508128.
    STRINGi4932.YBR261C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38340.
    SMRiP38340. Positions 11-228.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni123 – 1242S-adenosyl-L-methionine bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG278169.
    GeneTreeiENSGT00390000008371.
    KOiK16219.
    OMAiWCQWCVG.
    OrthoDBiEOG7W6WX8.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR008576. DUF858_MeTrfase_lik.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR12753. PTHR12753. 1 hit.
    PfamiPF05891. Methyltransf_PK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016958. DUF858_MeTrfase_lik. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P38340-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDVPADSHIK YEDAIDYWTD VDATVDGVLG GYGEGTVVPT MDVLGSNNFL    50
    RKLKSRMLPQ ENNVKYAVDI GAGIGRVSKT MLHKHAAKID LVEPVKPFIE 100
    QMHVELAELK DKGQIGQIYE VGMQDWTPDA GKYWLIWCQW CVGHLPDAEL 150
    VAFLKRCIVG LQPNGTIVVK ENNTPTDTDD FDETDSSVTR SDAKFRQIFE 200
    EAGLKLIASE RQRGLPRELY PVRMYALKPM PN 232
    Length:232
    Mass (Da):26,068
    Last modified:October 1, 1994 - v1
    Checksum:i66699F37B0013088
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70529 Genomic DNA. Translation: CAA49926.1.
    Z36130 Genomic DNA. Translation: CAA85224.1.
    BK006936 Genomic DNA. Translation: DAA07378.1.
    PIRiS32963.
    RefSeqiNP_009820.1. NM_001178609.1.

    Genome annotation databases

    EnsemblFungiiYBR261C; YBR261C; YBR261C.
    GeneIDi852564.
    KEGGisce:YBR261C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70529 Genomic DNA. Translation: CAA49926.1 .
    Z36130 Genomic DNA. Translation: CAA85224.1 .
    BK006936 Genomic DNA. Translation: DAA07378.1 .
    PIRi S32963.
    RefSeqi NP_009820.1. NM_001178609.1.

    3D structure databases

    ProteinModelPortali P38340.
    SMRi P38340. Positions 11-228.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32957. 79 interactions.
    DIPi DIP-4951N.
    IntActi P38340. 42 interactions.
    MINTi MINT-508128.
    STRINGi 4932.YBR261C.

    Proteomic databases

    MaxQBi P38340.
    PaxDbi P38340.
    PeptideAtlasi P38340.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR261C ; YBR261C ; YBR261C .
    GeneIDi 852564.
    KEGGi sce:YBR261C.

    Organism-specific databases

    CYGDi YBR261c.
    SGDi S000000465. TAE1.

    Phylogenomic databases

    eggNOGi NOG278169.
    GeneTreei ENSGT00390000008371.
    KOi K16219.
    OMAi WCQWCVG.
    OrthoDBi EOG7W6WX8.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29185-MONOMER.

    Miscellaneous databases

    NextBioi 971679.

    Gene expression databases

    Genevestigatori P38340.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR008576. DUF858_MeTrfase_lik.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR12753. PTHR12753. 1 hit.
    Pfami PF05891. Methyltransf_PK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016958. DUF858_MeTrfase_lik. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete sequence of a 19,482 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
      Doignon F., Biteau N., Crouzet M., Aigle M.
      Yeast 9:189-199(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Chemical-genetic profile analysis in yeast suggests that a previously uncharacterized open reading frame, YBR261C, affects protein synthesis."
      Alamgir M., Eroukova V., Jessulat M., Xu J., Golshani A.
      BMC Genomics 9:583-583(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    7. "Identification of protein N-terminal methyltransferases in yeast and humans."
      Webb K.J., Lipson R.S., Al-Hadid Q., Whitelegge J.P., Clarke S.G.
      Biochemistry 49:5225-5235(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiNTM1_YEAST
    AccessioniPrimary (citable) accession number: P38340
    Secondary accession number(s): D6VQQ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3060 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3