UniProtKB - P38340 (NTM1_YEAST)
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Protein
Alpha N-terminal protein methyltransferase 1
Gene
TAE1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Responsible for the N-terminal methylation of the ribosomal proteins RPL12A, RPL12B, RPS25A and RPS25B.1 Publication
Miscellaneous
Present with 3060 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein].
2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein].
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 71 | S-adenosyl-L-methionine; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 76 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 139 | S-adenosyl-L-methionine; via carbonyl oxygenBy similarity | 1 |
GO - Molecular functioni
- N-terminal protein N-methyltransferase activity Source: SGD
GO - Biological processi
- cytoplasmic translation Source: SGD
- N-terminal peptidyl-proline dimethylation Source: SGD
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | YEAST:G3O-29185-MONOMER. |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha N-terminal protein methyltransferase 1 (EC:2.1.1.244)Alternative name(s): Translation associated element 1 X-Pro-Lys N-terminal protein methyltransferase 1 Short name: NTM1 |
Gene namesi | Name:TAE1 Synonyms:NTM1 Ordered Locus Names:YBR261C ORF Names:YBR1729 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YBR261C. |
SGDi | S000000465. TAE1. |
Pathology & Biotechi
Disruption phenotypei
Defects in both translation efficiency and fidelity.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119292 | 1 – 232 | Alpha N-terminal protein methyltransferase 1Add BLAST | 232 |
Proteomic databases
MaxQBi | P38340. |
PaxDbi | P38340. |
PRIDEi | P38340. |
PTM databases
iPTMneti | P38340. |
Interactioni
Binary interactionsi
Protein-protein interaction databases
BioGridi | 32957. 111 interactors. |
DIPi | DIP-4951N. |
IntActi | P38340. 150 interactors. |
STRINGi | 4932.YBR261C. |
Structurei
3D structure databases
ProteinModelPortali | P38340. |
SMRi | P38340. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 123 – 124 | S-adenosyl-L-methionine bindingBy similarity | 2 |
Sequence similaritiesi
Phylogenomic databases
GeneTreei | ENSGT00390000008371. |
InParanoidi | P38340. |
KOi | K16219. |
OMAi | PVYMIAC. |
OrthoDBi | EOG092C2UR7. |
Family and domain databases
InterProi | View protein in InterPro IPR008576. MeTrfase_NTM1. IPR029063. SAM-dependent_MTases. |
PANTHERi | PTHR12753. PTHR12753. 1 hit. |
Pfami | View protein in Pfam PF05891. Methyltransf_PK. 1 hit. |
PIRSFi | PIRSF016958. DUF858_MeTrfase_lik. 1 hit. |
SUPFAMi | SSF53335. SSF53335. 1 hit. |
i Sequence
Sequence statusi: Complete.
P38340-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDVPADSHIK YEDAIDYWTD VDATVDGVLG GYGEGTVVPT MDVLGSNNFL
60 70 80 90 100
RKLKSRMLPQ ENNVKYAVDI GAGIGRVSKT MLHKHAAKID LVEPVKPFIE
110 120 130 140 150
QMHVELAELK DKGQIGQIYE VGMQDWTPDA GKYWLIWCQW CVGHLPDAEL
160 170 180 190 200
VAFLKRCIVG LQPNGTIVVK ENNTPTDTDD FDETDSSVTR SDAKFRQIFE
210 220 230
EAGLKLIASE RQRGLPRELY PVRMYALKPM PN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X70529 Genomic DNA. Translation: CAA49926.1. Z36130 Genomic DNA. Translation: CAA85224.1. BK006936 Genomic DNA. Translation: DAA07378.1. |
PIRi | S32963. |
RefSeqi | NP_009820.1. NM_001178609.1. |
Genome annotation databases
EnsemblFungii | YBR261C; YBR261C; YBR261C. |
GeneIDi | 852564. |
KEGGi | sce:YBR261C. |
Similar proteinsi
Entry informationi
Entry namei | NTM1_YEAST | |
Accessioni | P38340Primary (citable) accession number: P38340 Secondary accession number(s): D6VQQ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | March 28, 2018 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |