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Protein

Alpha N-terminal protein methyltransferase 1

Gene

TAE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Responsible for the N-terminal methylation of the ribosomal proteins RPL12A, RPL12B, RPS25A and RPS25B.1 Publication

Catalytic activityi

3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein].
2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein].

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei76 – 761S-adenosyl-L-methionineBy similarity
Binding sitei139 – 1391S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

GO - Molecular functioni

  1. N-terminal protein N-methyltransferase activity Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
  2. N-terminal peptidyl-proline dimethylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-29185-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha N-terminal protein methyltransferase 1 (EC:2.1.1.244)
Alternative name(s):
Translation associated element 1
X-Pro-Lys N-terminal protein methyltransferase 1
Short name:
NTM1
Gene namesi
Name:TAE1
Synonyms:NTM1
Ordered Locus Names:YBR261C
ORF Names:YBR1729
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome II

Organism-specific databases

CYGDiYBR261c.
EuPathDBiFungiDB:YBR261C.
SGDiS000000465. TAE1.

Subcellular locationi

  1. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Defects in both translation efficiency and fidelity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232Alpha N-terminal protein methyltransferase 1PRO_0000119292Add
BLAST

Proteomic databases

MaxQBiP38340.
PaxDbiP38340.
PeptideAtlasiP38340.

Expressioni

Gene expression databases

GenevestigatoriP38340.

Interactioni

Protein-protein interaction databases

BioGridi32957. 80 interactions.
DIPiDIP-4951N.
IntActiP38340. 42 interactions.
MINTiMINT-508128.
STRINGi4932.YBR261C.

Structurei

3D structure databases

ProteinModelPortaliP38340.
SMRiP38340. Positions 11-228.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1242S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG278169.
GeneTreeiENSGT00390000008371.
InParanoidiP38340.
KOiK16219.
OMAiGHLTDDH.
OrthoDBiEOG7W6WX8.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR008576. MeTrfase_NTM1.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR12753. PTHR12753. 1 hit.
PfamiPF05891. Methyltransf_PK. 1 hit.
[Graphical view]
PIRSFiPIRSF016958. DUF858_MeTrfase_lik. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

P38340-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVPADSHIK YEDAIDYWTD VDATVDGVLG GYGEGTVVPT MDVLGSNNFL
60 70 80 90 100
RKLKSRMLPQ ENNVKYAVDI GAGIGRVSKT MLHKHAAKID LVEPVKPFIE
110 120 130 140 150
QMHVELAELK DKGQIGQIYE VGMQDWTPDA GKYWLIWCQW CVGHLPDAEL
160 170 180 190 200
VAFLKRCIVG LQPNGTIVVK ENNTPTDTDD FDETDSSVTR SDAKFRQIFE
210 220 230
EAGLKLIASE RQRGLPRELY PVRMYALKPM PN
Length:232
Mass (Da):26,068
Last modified:October 1, 1994 - v1
Checksum:i66699F37B0013088
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70529 Genomic DNA. Translation: CAA49926.1.
Z36130 Genomic DNA. Translation: CAA85224.1.
BK006936 Genomic DNA. Translation: DAA07378.1.
PIRiS32963.
RefSeqiNP_009820.1. NM_001178609.1.

Genome annotation databases

EnsemblFungiiYBR261C; YBR261C; YBR261C.
GeneIDi852564.
KEGGisce:YBR261C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70529 Genomic DNA. Translation: CAA49926.1.
Z36130 Genomic DNA. Translation: CAA85224.1.
BK006936 Genomic DNA. Translation: DAA07378.1.
PIRiS32963.
RefSeqiNP_009820.1. NM_001178609.1.

3D structure databases

ProteinModelPortaliP38340.
SMRiP38340. Positions 11-228.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32957. 80 interactions.
DIPiDIP-4951N.
IntActiP38340. 42 interactions.
MINTiMINT-508128.
STRINGi4932.YBR261C.

Proteomic databases

MaxQBiP38340.
PaxDbiP38340.
PeptideAtlasiP38340.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR261C; YBR261C; YBR261C.
GeneIDi852564.
KEGGisce:YBR261C.

Organism-specific databases

CYGDiYBR261c.
EuPathDBiFungiDB:YBR261C.
SGDiS000000465. TAE1.

Phylogenomic databases

eggNOGiNOG278169.
GeneTreeiENSGT00390000008371.
InParanoidiP38340.
KOiK16219.
OMAiGHLTDDH.
OrthoDBiEOG7W6WX8.

Enzyme and pathway databases

BioCyciYEAST:G3O-29185-MONOMER.

Miscellaneous databases

NextBioi971679.
PROiP38340.

Gene expression databases

GenevestigatoriP38340.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR008576. MeTrfase_NTM1.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR12753. PTHR12753. 1 hit.
PfamiPF05891. Methyltransf_PK. 1 hit.
[Graphical view]
PIRSFiPIRSF016958. DUF858_MeTrfase_lik. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete sequence of a 19,482 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
    Doignon F., Biteau N., Crouzet M., Aigle M.
    Yeast 9:189-199(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Chemical-genetic profile analysis in yeast suggests that a previously uncharacterized open reading frame, YBR261C, affects protein synthesis."
    Alamgir M., Eroukova V., Jessulat M., Xu J., Golshani A.
    BMC Genomics 9:583-583(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "Identification of protein N-terminal methyltransferases in yeast and humans."
    Webb K.J., Lipson R.S., Al-Hadid Q., Whitelegge J.P., Clarke S.G.
    Biochemistry 49:5225-5235(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNTM1_YEAST
AccessioniPrimary (citable) accession number: P38340
Secondary accession number(s): D6VQQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 29, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3060 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.