Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P38340 (NTM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha N-terminal protein methyltransferase 1

EC=2.1.1.244
Alternative name(s):
Translation associated element 1
X-Pro-Lys N-terminal protein methyltransferase 1
Short name=NTM1
Gene names
Name:TAE1
Synonyms:NTM1
Ordered Locus Names:YBR261C
ORF Names:YBR1729
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length232 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Responsible for the N-terminal methylation of the ribosomal proteins RPL12A, RPL12B, RPS25A and RPS25B. Ref.7

Catalytic activity

3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein].

2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein].

Subcellular location

Cytoplasm Ref.4.

Disruption phenotype

Defects in both translation efficiency and fidelity. Ref.6

Miscellaneous

Present with 3060 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the methyltransferase superfamily. NTM1 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 232232Alpha N-terminal protein methyltransferase 1
PRO_0000119292

Regions

Region123 – 1242S-adenosyl-L-methionine binding By similarity

Sites

Binding site711S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site761S-adenosyl-L-methionine By similarity
Binding site1391S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
P38340 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 66699F37B0013088

FASTA23226,068
        10         20         30         40         50         60 
MDVPADSHIK YEDAIDYWTD VDATVDGVLG GYGEGTVVPT MDVLGSNNFL RKLKSRMLPQ 

        70         80         90        100        110        120 
ENNVKYAVDI GAGIGRVSKT MLHKHAAKID LVEPVKPFIE QMHVELAELK DKGQIGQIYE 

       130        140        150        160        170        180 
VGMQDWTPDA GKYWLIWCQW CVGHLPDAEL VAFLKRCIVG LQPNGTIVVK ENNTPTDTDD 

       190        200        210        220        230 
FDETDSSVTR SDAKFRQIFE EAGLKLIASE RQRGLPRELY PVRMYALKPM PN 

« Hide

References

« Hide 'large scale' references
[1]"The complete sequence of a 19,482 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
Doignon F., Biteau N., Crouzet M., Aigle M.
Yeast 9:189-199(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Chemical-genetic profile analysis in yeast suggests that a previously uncharacterized open reading frame, YBR261C, affects protein synthesis."
Alamgir M., Eroukova V., Jessulat M., Xu J., Golshani A.
BMC Genomics 9:583-583(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[7]"Identification of protein N-terminal methyltransferases in yeast and humans."
Webb K.J., Lipson R.S., Al-Hadid Q., Whitelegge J.P., Clarke S.G.
Biochemistry 49:5225-5235(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X70529 Genomic DNA. Translation: CAA49926.1.
Z36130 Genomic DNA. Translation: CAA85224.1.
BK006936 Genomic DNA. Translation: DAA07378.1.
PIRS32963.
RefSeqNP_009820.1. NM_001178609.1.

3D structure databases

ProteinModelPortalP38340.
SMRP38340. Positions 11-228.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32957. 79 interactions.
DIPDIP-4951N.
IntActP38340. 42 interactions.
MINTMINT-508128.
STRING4932.YBR261C.

Proteomic databases

MaxQBP38340.
PaxDbP38340.
PeptideAtlasP38340.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR261C; YBR261C; YBR261C.
GeneID852564.
KEGGsce:YBR261C.

Organism-specific databases

CYGDYBR261c.
SGDS000000465. YBR261C.

Phylogenomic databases

eggNOGNOG278169.
GeneTreeENSGT00390000008371.
KOK16219.
OMAWCQWCVG.
OrthoDBEOG7W6WX8.

Enzyme and pathway databases

BioCycYEAST:G3O-29185-MONOMER.

Gene expression databases

GenevestigatorP38340.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR008576. DUF858_MeTrfase_lik.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR12753. PTHR12753. 1 hit.
PfamPF05891. Methyltransf_PK. 1 hit.
[Graphical view]
PIRSFPIRSF016958. DUF858_MeTrfase_lik. 1 hit.
SUPFAMSSF53335. SSF53335. 1 hit.
ProtoNetSearch...

Other

NextBio971679.

Entry information

Entry nameNTM1_YEAST
AccessionPrimary (citable) accession number: P38340
Secondary accession number(s): D6VQQ8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 11, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families