P38340 (NTM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha N-terminal protein methyltransferase 1
EC=2.1.1.244

Alternative name(s):
Translation associated element 1
X-Pro-Lys N-terminal protein methyltransferase 1
Short name=NTM1

Gene names

Name:	TAE1
Synonyms:	NTM1
Ordered Locus Names:	YBR261C
ORF Names:	YBR1729

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Responsible for the N-terminal methylation of the ribosomal proteins RPL12A, RPL12B, RPS25A and RPS25B. Ref.7
Catalytic activity	3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein]. 2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein].
Subcellular location	Cytoplasm Ref.4.
Disruption phenotype	Defects in both translation efficiency and fidelity. Ref.6
Miscellaneous	Present with 3060 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the methyltransferase superfamily. NTM1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-terminal peptidyl-proline dimethylation Inferred from direct assay Ref.7. Source: SGD cytoplasmic translation Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	cytosol Inferred from direct assay Ref.7. Source: SGD
Molecular_function	N-terminal protein N-methyltransferase activity Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	Alpha N-terminal protein methyltransferase 1		PRO_0000119292
Regions
	Region	123 – 124	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	71	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	76	1	S-adenosyl-L-methionine By similarity
	Binding site	139	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38340 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 66699F37B0013088	FASTA	232	26,068
	10 20 30 40 50 60 MDVPADSHIK YEDAIDYWTD VDATVDGVLG GYGEGTVVPT MDVLGSNNFL RKLKSRMLPQ 70 80 90 100 110 120 ENNVKYAVDI GAGIGRVSKT MLHKHAAKID LVEPVKPFIE QMHVELAELK DKGQIGQIYE 130 140 150 160 170 180 VGMQDWTPDA GKYWLIWCQW CVGHLPDAEL VAFLKRCIVG LQPNGTIVVK ENNTPTDTDD 190 200 210 220 230 FDETDSSVTR SDAKFRQIFE EAGLKLIASE RQRGLPRELY PVRMYALKPM PN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequence of a 19,482 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae." Doignon F., Biteau N., Crouzet M., Aigle M. Yeast 9:189-199(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The reference genome sequence of Saccharomyces cerevisiae: Then and now." Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M. G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Chemical-genetic profile analysis in yeast suggests that a previously uncharacterized open reading frame, YBR261C, affects protein synthesis." Alamgir M., Eroukova V., Jessulat M., Xu J., Golshani A. BMC Genomics 9:583-583(2008) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[7]	"Identification of protein N-terminal methyltransferases in yeast and humans." Webb K.J., Lipson R.S., Al-Hadid Q., Whitelegge J.P., Clarke S.G. Biochemistry 49:5225-5235(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X70529 Genomic DNA. Translation: CAA49926.1. Z36130 Genomic DNA. Translation: CAA85224.1. BK006936 Genomic DNA. Translation: DAA07378.1.
PIR	S32963.
RefSeq	NP_009820.1. NM_001178609.1.
3D structure databases
ProteinModelPortal	P38340.
SMR	P38340. Positions 11-228.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	32957. 79 interactions.
DIP	DIP-4951N.
IntAct	P38340. 42 interactions.
MINT	MINT-508128.
STRING	4932.YBR261C.
Proteomic databases
MaxQB	P38340.
PaxDb	P38340.
PeptideAtlas	P38340.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR261C; YBR261C; YBR261C.
GeneID	852564.
KEGG	sce:YBR261C.
Organism-specific databases
CYGD	YBR261c.
SGD	S000000465. YBR261C.
Phylogenomic databases
eggNOG	NOG278169.
GeneTree	ENSGT00390000008371.
KO	K16219.
OMA	WCQWCVG.
OrthoDB	EOG7W6WX8.
Enzyme and pathway databases
BioCyc	YEAST:G3O-29185-MONOMER.
Gene expression databases
Genevestigator	P38340.
Family and domain databases
Gene3D	3.40.50.150. 1 hit.
InterPro	IPR008576. DUF858_MeTrfase_lik. IPR029063. SAM-dependent_MTases-like. [Graphical view]
PANTHER	PTHR12753. PTHR12753. 1 hit.
Pfam	PF05891. Methyltransf_PK. 1 hit. [Graphical view]
PIRSF	PIRSF016958. DUF858_MeTrfase_lik. 1 hit.
SUPFAM	SSF53335. SSF53335. 1 hit.
ProtoNet	Search...
Other
NextBio	971679.

Entry information

Entry name

NTM1_YEAST

Accession

Primary (citable) accession number: P38340
Secondary accession number(s): D6VQQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 11, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program