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P38334

- TRS20_YEAST

UniProt

P38334 - TRS20_YEAST

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Protein

Trafficking protein particle complex subunit 20

Gene

TRS20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation.4 Publications

GO - Molecular functioni

  1. identical protein binding Source: IntAct

GO - Biological processi

  1. autophagy Source: UniProtKB-KW
  2. ER to Golgi vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, ER-Golgi transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29179-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Trafficking protein particle complex subunit 20
Short name:
TRAPP subunit 20
Alternative name(s):
Transport protein particle 20 kDa subunit
Gene namesi
Name:TRS20
Ordered Locus Names:YBR254C
ORF Names:YBR1722
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR254c.
SGDiS000000458. TRS20.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. TRAPPIII protein complex Source: SGD
  3. TRAPPII protein complex Source: SGD
  4. TRAPPI protein complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 175175Trafficking protein particle complex subunit 20PRO_0000211568Add
BLAST

Proteomic databases

MaxQBiP38334.
PaxDbiP38334.

Expressioni

Gene expression databases

GenevestigatoriP38334.

Interactioni

Subunit structurei

Part of the multisubunit TRAPP (transport protein particle) I complex composed of BET3, BET5, TRS20, TRS23, TRS31 and TRS33. Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS85, TRS120 and TRS130. Part of the multisubunit TRAPP (transport protein particle) III complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33 and TRS85.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-19468,EBI-19468
BET3P3614911EBI-19468,EBI-3567
TRS130Q036603EBI-19468,EBI-19461
TRS31Q033375EBI-19468,EBI-38770
TRS85P469445EBI-19468,EBI-19492

Protein-protein interaction databases

BioGridi32949. 129 interactions.
DIPiDIP-1711N.
IntActiP38334. 21 interactions.
MINTiMINT-407365.
STRINGi4932.YBR254C.

Structurei

3D structure databases

ProteinModelPortaliP38334.
SMRiP38334. Positions 4-173.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5603.
GeneTreeiENSGT00510000047168.
HOGENOMiHOG000214139.
InParanoidiP38334.
OMAiGHNDNPI.
OrthoDBiEOG722JN6.

Family and domain databases

InterProiIPR011012. Longin-like_dom.
IPR006722. Sedlin.
[Graphical view]
PANTHERiPTHR12403. PTHR12403. 1 hit.
PfamiPF04628. Sedlin_N. 1 hit.
[Graphical view]
SUPFAMiSSF64356. SSF64356. 2 hits.

Sequencei

Sequence statusi: Complete.

P38334-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPQYFAIIGK KDNPVYEIEF TNAENPQGFP QDLKELNPFI LHASLDIVED
60 70 80 90 100
LQWQINPTSQ LNGNGGNGSN GGGGFLRSRA VNNTDNCYLG KVDHFYGLAI
110 120 130 140 150
TAYISYSGMK FVMIHGNSAN SSVVIDDNNM RSFYQEVHEL YVKTLMNPFY
160 170
KITDPIRSPA FDSRVRTLAR KHLSK
Length:175
Mass (Da):19,700
Last modified:October 1, 1994 - v1
Checksum:i82285CAEAC2735D9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X70529 Genomic DNA. Translation: CAA49918.1.
Z36123 Genomic DNA. Translation: CAA85217.1.
BK006936 Genomic DNA. Translation: DAA07370.1.
PIRiS32957.
RefSeqiNP_009813.1. NM_001178602.1.

Genome annotation databases

EnsemblFungiiYBR254C; YBR254C; YBR254C.
GeneIDi852556.
KEGGisce:YBR254C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X70529 Genomic DNA. Translation: CAA49918.1 .
Z36123 Genomic DNA. Translation: CAA85217.1 .
BK006936 Genomic DNA. Translation: DAA07370.1 .
PIRi S32957.
RefSeqi NP_009813.1. NM_001178602.1.

3D structure databases

ProteinModelPortali P38334.
SMRi P38334. Positions 4-173.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32949. 129 interactions.
DIPi DIP-1711N.
IntActi P38334. 21 interactions.
MINTi MINT-407365.
STRINGi 4932.YBR254C.

Proteomic databases

MaxQBi P38334.
PaxDbi P38334.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR254C ; YBR254C ; YBR254C .
GeneIDi 852556.
KEGGi sce:YBR254C.

Organism-specific databases

CYGDi YBR254c.
SGDi S000000458. TRS20.

Phylogenomic databases

eggNOGi COG5603.
GeneTreei ENSGT00510000047168.
HOGENOMi HOG000214139.
InParanoidi P38334.
OMAi GHNDNPI.
OrthoDBi EOG722JN6.

Enzyme and pathway databases

BioCyci YEAST:G3O-29179-MONOMER.

Miscellaneous databases

NextBioi 971655.
PROi P38334.

Gene expression databases

Genevestigatori P38334.

Family and domain databases

InterProi IPR011012. Longin-like_dom.
IPR006722. Sedlin.
[Graphical view ]
PANTHERi PTHR12403. PTHR12403. 1 hit.
Pfami PF04628. Sedlin_N. 1 hit.
[Graphical view ]
SUPFAMi SSF64356. SSF64356. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete sequence of a 19,482 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
    Doignon F., Biteau N., Crouzet M., Aigle M.
    Yeast 9:189-199(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion."
    Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.
    EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE TRAPP II COMPLEX.
  5. "TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport."
    Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S.
    Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, FUNCTION OF THE TRAPP I COMPLEX, IDENTIFICATION IN THE TRAPP I COMPLEX, SUBCELLULAR LOCATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Molecular architecture of the TRAPPII complex and implications for vesicle tethering."
    Yip C.K., Berscheminski J., Walz T.
    Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
  8. Cited for: IDENTIFICATION IN THE TRAPP III COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE TRAPP III COMPLEX.

Entry informationi

Entry nameiTRS20_YEAST
AccessioniPrimary (citable) accession number: P38334
Secondary accession number(s): D6VQQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3