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P38334

- TRS20_YEAST

UniProt

P38334 - TRS20_YEAST

Protein

Trafficking protein particle complex subunit 20

Gene

TRS20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation.4 Publications

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct

    GO - Biological processi

    1. autophagy Source: UniProtKB-KW
    2. ER to Golgi vesicle-mediated transport Source: SGD

    Keywords - Biological processi

    Autophagy, ER-Golgi transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29179-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trafficking protein particle complex subunit 20
    Short name:
    TRAPP subunit 20
    Alternative name(s):
    Transport protein particle 20 kDa subunit
    Gene namesi
    Name:TRS20
    Ordered Locus Names:YBR254C
    ORF Names:YBR1722
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR254c.
    SGDiS000000458. TRS20.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-SubCell
    2. pre-autophagosomal structure Source: UniProtKB-SubCell
    3. TRAPPIII protein complex Source: SGD
    4. TRAPPII protein complex Source: SGD
    5. TRAPPI protein complex Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 175175Trafficking protein particle complex subunit 20PRO_0000211568Add
    BLAST

    Proteomic databases

    MaxQBiP38334.
    PaxDbiP38334.

    Expressioni

    Gene expression databases

    GenevestigatoriP38334.

    Interactioni

    Subunit structurei

    Part of the multisubunit TRAPP (transport protein particle) I complex composed of BET3, BET5, TRS20, TRS23, TRS31 and TRS33. Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS85, TRS120 and TRS130. Part of the multisubunit TRAPP (transport protein particle) III complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33 and TRS85.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-19468,EBI-19468
    BET3P3614911EBI-19468,EBI-3567
    TRS130Q036603EBI-19468,EBI-19461
    TRS31Q033375EBI-19468,EBI-38770
    TRS85P469445EBI-19468,EBI-19492

    Protein-protein interaction databases

    BioGridi32949. 128 interactions.
    DIPiDIP-1711N.
    IntActiP38334. 21 interactions.
    MINTiMINT-407365.
    STRINGi4932.YBR254C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38334.
    SMRiP38334. Positions 4-173.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG5603.
    GeneTreeiENSGT00510000047168.
    HOGENOMiHOG000214139.
    OMAiGHNDNPI.
    OrthoDBiEOG722JN6.

    Family and domain databases

    InterProiIPR011012. Longin-like_dom.
    IPR006722. Sedlin.
    [Graphical view]
    PANTHERiPTHR12403. PTHR12403. 1 hit.
    PfamiPF04628. Sedlin_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF64356. SSF64356. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P38334-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPQYFAIIGK KDNPVYEIEF TNAENPQGFP QDLKELNPFI LHASLDIVED    50
    LQWQINPTSQ LNGNGGNGSN GGGGFLRSRA VNNTDNCYLG KVDHFYGLAI 100
    TAYISYSGMK FVMIHGNSAN SSVVIDDNNM RSFYQEVHEL YVKTLMNPFY 150
    KITDPIRSPA FDSRVRTLAR KHLSK 175
    Length:175
    Mass (Da):19,700
    Last modified:October 1, 1994 - v1
    Checksum:i82285CAEAC2735D9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70529 Genomic DNA. Translation: CAA49918.1.
    Z36123 Genomic DNA. Translation: CAA85217.1.
    BK006936 Genomic DNA. Translation: DAA07370.1.
    PIRiS32957.
    RefSeqiNP_009813.1. NM_001178602.1.

    Genome annotation databases

    EnsemblFungiiYBR254C; YBR254C; YBR254C.
    GeneIDi852556.
    KEGGisce:YBR254C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70529 Genomic DNA. Translation: CAA49918.1 .
    Z36123 Genomic DNA. Translation: CAA85217.1 .
    BK006936 Genomic DNA. Translation: DAA07370.1 .
    PIRi S32957.
    RefSeqi NP_009813.1. NM_001178602.1.

    3D structure databases

    ProteinModelPortali P38334.
    SMRi P38334. Positions 4-173.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32949. 128 interactions.
    DIPi DIP-1711N.
    IntActi P38334. 21 interactions.
    MINTi MINT-407365.
    STRINGi 4932.YBR254C.

    Proteomic databases

    MaxQBi P38334.
    PaxDbi P38334.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR254C ; YBR254C ; YBR254C .
    GeneIDi 852556.
    KEGGi sce:YBR254C.

    Organism-specific databases

    CYGDi YBR254c.
    SGDi S000000458. TRS20.

    Phylogenomic databases

    eggNOGi COG5603.
    GeneTreei ENSGT00510000047168.
    HOGENOMi HOG000214139.
    OMAi GHNDNPI.
    OrthoDBi EOG722JN6.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29179-MONOMER.

    Miscellaneous databases

    NextBioi 971655.
    PROi P38334.

    Gene expression databases

    Genevestigatori P38334.

    Family and domain databases

    InterProi IPR011012. Longin-like_dom.
    IPR006722. Sedlin.
    [Graphical view ]
    PANTHERi PTHR12403. PTHR12403. 1 hit.
    Pfami PF04628. Sedlin_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF64356. SSF64356. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The complete sequence of a 19,482 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
      Doignon F., Biteau N., Crouzet M., Aigle M.
      Yeast 9:189-199(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion."
      Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.
      EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE TRAPP II COMPLEX.
    5. "TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport."
      Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S.
      Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, FUNCTION OF THE TRAPP I COMPLEX, IDENTIFICATION IN THE TRAPP I COMPLEX, SUBCELLULAR LOCATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Molecular architecture of the TRAPPII complex and implications for vesicle tethering."
      Yip C.K., Berscheminski J., Walz T.
      Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
    8. Cited for: IDENTIFICATION IN THE TRAPP III COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE TRAPP III COMPLEX.

    Entry informationi

    Entry nameiTRS20_YEAST
    AccessioniPrimary (citable) accession number: P38334
    Secondary accession number(s): D6VQQ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1200 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3