P38334 (TRS20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trafficking protein particle complex subunit 20
Short name=TRAPP subunit 20

Alternative name(s):
Transport protein particle 20 kDa subunit

Gene names

Name:	TRS20
Ordered Locus Names:	YBR254C
ORF Names:	YBR1722

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. Ref.4 Ref.5 Ref.7 Ref.8
Subunit structure	Part of the multisubunit TRAPP (transport protein particle) I complex composed of BET3, BET5, TRS20, TRS23, TRS31 and TRS33. Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS85, TRS120 and TRS130. Part of the multisubunit TRAPP (transport protein particle) III complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33 and TRS85. Ref.4 Ref.5 Ref.7 Ref.8
Subcellular location	Golgi apparatus › cis-Golgi network. Endoplasmic reticulum. Preautophagosomal structure Ref.5 Ref.8.
Miscellaneous	Present with 1200 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the TRAPP small subunits family. Sedlin subfamily.

Ontologies

Keywords
Biological process	Autophagy ER-Golgi transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from mutant phenotype Ref.5. Source: SGD autophagy Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	TRAPP complex Inferred from direct assay PubMed 10727015Ref.4. Source: SGD endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell pre-autophagosomal structure Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BET3	P36149	11	EBI-19468,EBI-3567
TRS31	Q03337	5	EBI-19468,EBI-38770

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 175	175	Trafficking protein particle complex subunit 20		PRO_0000211568

Sequences

	Sequence		Length	Mass (Da)
	P38334 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 82285CAEAC2735D9	FASTA	175	19,700
	10 20 30 40 50 60 MPQYFAIIGK KDNPVYEIEF TNAENPQGFP QDLKELNPFI LHASLDIVED LQWQINPTSQ 70 80 90 100 110 120 LNGNGGNGSN GGGGFLRSRA VNNTDNCYLG KVDHFYGLAI TAYISYSGMK FVMIHGNSAN 130 140 150 160 170 SSVVIDDNNM RSFYQEVHEL YVKTLMNPFY KITDPIRSPA FDSRVRTLAR KHLSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequence of a 19,482 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae." Doignon F., Biteau N., Crouzet M., Aigle M. Yeast 9:189-199(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion." Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S. EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE TRAPP II COMPLEX.
[5]	"TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport." Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S. Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, FUNCTION OF THE TRAPP I COMPLEX, IDENTIFICATION IN THE TRAPP I COMPLEX, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Molecular architecture of the TRAPPII complex and implications for vesicle tethering." Yip C.K., Berscheminski J., Walz T. Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
[8]	"Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy." Lynch-Day M.A., Bhandari D., Menon S., Huang J., Cai H., Bartholomew C.R., Brumell J.H., Ferro-Novick S., Klionsky D.J. Proc. Natl. Acad. Sci. U.S.A. 107:7811-7816(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TRAPP III COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE TRAPP III COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X70529 Genomic DNA. Translation: CAA49918.1. Z36123 Genomic DNA. Translation: CAA85217.1. BK006936 Genomic DNA. Translation: DAA07370.1.
PIR	S32957.
RefSeq	NP_009813.1. NM_001178602.1.
3D structure databases
ProteinModelPortal	P38334.
SMR	P38334. Positions 4-173.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1711N.
IntAct	P38334. 20 interactions.
MINT	MINT-407365.
STRING	4932.YBR254C.
Proteomic databases
PaxDb	P38334.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR254C; YBR254C; YBR254C.
GeneID	852556.
KEGG	sce:YBR254C.
Organism-specific databases
CYGD	YBR254c.
SGD	S000000458. TRS20.
Phylogenomic databases
eggNOG	COG5603.
GeneTree	ENSGT00510000047168.
HOGENOM	HOG000214139.
OMA	VGHNDNP.
OrthoDB	EOG479JHG.
Enzyme and pathway databases
BioCyc	YEAST:G3O-29179-MONOMER.
Gene expression databases
Genevestigator	P38334.
GermOnline	YBR254C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011012. Longin-like_dom. IPR006722. Sedlin. [Graphical view]
PANTHER	PTHR12403. PTHR12403. 1 hit.
Pfam	PF04628. Sedlin_N. 1 hit. [Graphical view]
SUPFAM	SSF64356. Longin_like. 1 hit.
ProtoNet	Search...
Other
NextBio	971655.

Entry information

Entry name

TRS20_YEAST

Accession

Primary (citable) accession number: P38334
Secondary accession number(s): D6VQQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 29, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program