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Protein

Vacuolar cation-chloride cotransporter 1

Gene

VHC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the coordinated symport of chloride with potassium ions across the vacuolar membrane. Involved in vacuolar osmoregulation.1 Publication

GO - Molecular functioni

  • potassium:chloride symporter activity Source: SGD
  • potassium ion symporter activity Source: GO_Central

GO - Biological processi

  • calcium ion transport Source: UniProtKB-KW
  • potassium ion homeostasis Source: SGD
  • vacuolar transmembrane transport Source: SGD

Keywordsi

Biological processCalcium transport, Ion transport, Potassium transport, Transport
LigandCalcium, Potassium

Enzyme and pathway databases

BioCyciYEAST:G3O-29166-MONOMER.
ReactomeiR-SCE-426117. Cation-coupled Chloride cotransporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar cation-chloride cotransporter 11 Publication
Alternative name(s):
Vacuolar homolog of CCC family protein 11 Publication
Gene namesi
Name:VHC11 Publication
Ordered Locus Names:YBR235WImported
ORF Names:YBR1601
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR235W.
SGDiS000000439. VHC1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 62Cytoplasmic1 PublicationAdd BLAST62
Transmembranei63 – 83Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini84 – 85Vacuolar1 Publication2
Transmembranei86 – 106Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini107 – 145Cytoplasmic1 PublicationAdd BLAST39
Transmembranei146 – 166Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini167 – 193Vacuolar1 PublicationAdd BLAST27
Transmembranei194 – 214Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini215 – 221Cytoplasmic1 Publication7
Transmembranei222 – 242Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini243 – 283Vacuolar1 PublicationAdd BLAST41
Transmembranei284 – 304Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini305 – 317Cytoplasmic1 PublicationAdd BLAST13
Transmembranei318 – 338Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini339 – 360Vacuolar1 PublicationAdd BLAST22
Transmembranei361 – 381Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini382 – 393Cytoplasmic1 PublicationAdd BLAST12
Transmembranei394 – 414Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini415 – 430Vacuolar1 PublicationAdd BLAST16
Transmembranei431 – 451Helical; Name=10Sequence analysisAdd BLAST21
Topological domaini452 – 462Cytoplasmic1 PublicationAdd BLAST11
Transmembranei463 – 482Helical; Name=11Sequence analysisAdd BLAST20
Topological domaini483 – 487Vacuolar1 Publication5
Transmembranei488 – 506Helical; Name=12Sequence analysisAdd BLAST19
Topological domaini507 – 1120Cytoplasmic1 PublicationAdd BLAST614

GO - Cellular componenti

  • integral component of vacuolar membrane Source: SGD
  • vacuole-mitochondrion membrane contact site Source: SGD

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

Affects vacuolar morphology and decreases survival upon hyperosmotic stress.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002025201 – 1120Vacuolar cation-chloride cotransporter 1Add BLAST1120

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphoserineCombined sources1
Modified residuei654PhosphoserineCombined sources1
Modified residuei915PhosphoserineCombined sources1
Modified residuei918PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38329.
PRIDEiP38329.

PTM databases

iPTMnetiP38329.

Interactioni

Protein-protein interaction databases

BioGridi32930. 74 interactors.
DIPiDIP-2969N.
MINTiMINT-2493986.
STRINGi4932.YBR235W.

Structurei

3D structure databases

ProteinModelPortaliP38329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SLC12A transporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119053.
HOGENOMiHOG000188913.
InParanoidiP38329.
KOiK14429.
OMAiDLWPIQM.
OrthoDBiEOG092C0G1J.

Family and domain databases

InterProiView protein in InterPro
IPR004841. AA-permease/SLC12A_dom.
IPR018491. SLC12_C.
PfamiView protein in Pfam
PF00324. AA_permease. 1 hit.
PF03522. SLC12. 1 hit.

Sequencei

Sequence statusi: Complete.

P38329-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSRFYQIPG THRPSSAISS SNESSSLLSA RRISQTYFNY QATPECQKVS
60 70 80 90 100
SKYDPDNPNK DKLGTYDGVF VPTALNVLSI LMFLRFGFIL GQLGIICTIG
110 120 130 140 150
LLLLSYTINL LTTLSISAIS TNGTVRGGGA YYMISRSLGP EFGGSIGLVF
160 170 180 190 200
FLGQVFNAGM NAVGIIEPLL YNLGYSAQGE PPAALGELLP RGHWHEFTYA
210 220 230 240 250
TVILFLCFSV AFVGSQTVSR AGNILFLVLA ASIFSIPLSA LIRSPFTEGG
260 270 280 290 300
ISYTGPSWQT FHDNLLPHLT KGAAGSLLKG KETFNDLFGV FFPATAGIFA
310 320 330 340 350
GAGMSSELRK PSKSIPKGTL WGLLFTFICY AVVVFSMGCS IPRRSLYDEV
360 370 380 390 400
QIIQTISSVQ WVIFMGEMAT SLFSIIVGML GAAYVLEAIA KDNIIPGLEI
410 420 430 440 450
FAHSPLYSLI FTWILTQLCL FSDVNKIATF ITMTFLMTFV VMNLACFLLG
460 470 480 490 500
ISSAPNFRPS FKYFNRYTTA IGALLSVVAM LIVDGISASV LFLAMILLFL
510 520 530 540 550
FIHYFSPPKS WGDVSQSLIY HQVRKYLLRL RQDNIKYWRP QILLFVDNPR
560 570 580 590 600
TSWNLIRFCN HLKKGGLYIL GHVAVTADFP KQLNELKTQQ KAWMKIRDMA
610 620 630 640 650
AIKAFVQVGT GPSLIWGIRN VFIGSGLGGM KPNITVVGFF DLESYRKHIP
660 670 680 690 700
QSRSQNNLQK QVEIKATVPR STCSDVKINV PLPTDECKNE TKVNVQQWVQ
710 720 730 740 750
IVEDLSLMQS NIAIAHGFKN LEIPNKRDSC FPKKTIDLYP IQMCGKVEAK
760 770 780 790 800
GDQPAAITTN FDTYTLILQL AAILVTVPEW KRTHSLRVIL FVEQEYHRTN
810 820 830 840 850
ETQRMKKLLQ VLRIDAEVLV VSLDQFRVYN TIVKGDPIVF DYVNSKLADN
860 870 880 890 900
EWWKDLVEAR DTLKPKRRFS TIEPQTIAKQ FTQSRKYTSG VQKLGVSFTM
910 920 930 940 950
NTRMPTNRID TPCESEDSDL DTDLTSIRDA FSASTNISVG KDLTTKSKTG
960 970 980 990 1000
SDRTNLLVKN LQSDVSTQSL RPVFSSNTLP RTRVVEDGTG EQPTLIPIAE
1010 1020 1030 1040 1050
PDLSNGNGTG SGIGNGNKLK KPVLPELSPC CSKDSLVTAM QNLGFNDLPS
1060 1070 1080 1090 1100
TAQHLVLNDV MTQMSKSSDL IFSTLPVPAL GTHEDHDASL QYVEDLDIWL
1110 1120
EGLPPCMLIN SQTMTVTTAL
Length:1,120
Mass (Da):123,999
Last modified:October 1, 1994 - v1
Checksum:i299C99C0CBC3E5CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36104 Genomic DNA. Translation: CAA85198.1.
BK006936 Genomic DNA. Translation: DAA07351.1.
PIRiS46111.
RefSeqiNP_009794.3. NM_001178583.3.

Genome annotation databases

EnsemblFungiiYBR235W; YBR235W; YBR235W.
GeneIDi852537.
KEGGisce:YBR235W.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiVHC1_YEAST
AccessioniPrimary (citable) accession number: P38329
Secondary accession number(s): D6VQN1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 5, 2017
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names