P38315 (YBP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
YAP1-binding protein 1

Alternative name(s):
Activator of YAP1

Gene names

Name:	YBP1
Ordered Locus Names:	YBR216C
ORF Names:	YBR1505

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	674 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Invoved in oxidative stress response and redox homeostasis. Required for hydrogen peroxide-induced oxidation and nuclear localization (activation) of YAP1. Functions probably in concert with HYP1/GPX3, the actual YAP1 modifying enzyme. YBP1 is not required for HYP1/GPX3-independent, diamide-induced oxidation of YAP1. Ref.3 Ref.4
Subunit structure	Interacts with YAP1. Forms a peroxide stress induced complex with YAP1 in the cytoplasm. Systematic proteome-wide 2-hybrid interaction studies suggest that YAP1, HYR1/GPX3, and YBP1 all interact with the nuclear pore complex subunit NUP116, which is involved in nucleocytoplasmic transport. Ref.3
Subcellular location	Cytoplasm Ref.5.
Miscellaneous	Present with 3100 molecules/cell in log phase SD medium.
Sequence similarities	To yeast YBP2.
Caution	YBP1 encoded by the widely used laboratory strain W303-1a is only partially functional, probably due to four amino acid substitutions.

Ontologies

Keywords
Cellular component	Cytoplasm
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to oxidative stress Inferred from direct assay Ref.4. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YAP1	P19880	4	EBI-20985,EBI-31265

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 674	674	YAP1-binding protein 1		PRO_0000066150
Regions
	Compositional bias	613 – 616	4	Poly-Leu
Natural variations
	Natural variant	7	1	I → L in strain: W303-1a.
	Natural variant	328	1	F → V in strain: W303-1a.
	Natural variant	343	1	K → E in strain: W303-1a.
	Natural variant	570	1	N → D in strain: W303-1a.

Sequences

	Sequence		Length	Mass (Da)
	P38315 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 31F2B200CE34B32D	FASTA	674	77,741
	10 20 30 40 50 60 MEPIDDILFE VTDAFKTQKE DLLELVTLID IYGEQVNQEG SYEEKTRFIE TLNTLLEDNP 70 80 90 100 110 120 STTGEIGWDL PKGLLKFLSK DNVDVNGRLG TNMIVQGVMK CFYAISIQGE PKKCLITGLE 130 140 150 160 170 180 LLSSLCSKDF SKSDQQNKED FVDKKANTLP PEGVIENSSN RKDFPSYGES KSSNEFFLKL 190 200 210 220 230 240 KSYILFEFIG ASLKRISTLF PSKYLGAAVS TIEKFVYSHA DTFEDALFLL RRVYTFCRNY 250 260 270 280 290 300 IPPDPPKDIQ LNEDFTREMF DKVVEEESEL QVRLLRRLCT FGISTPIKTV TTNADVKYYC 310 320 330 340 350 360 ALNQQKFELS AYYTEYLELF CRYYQMAFSL DVDIEGEFQN VIKECRIIYK SVPQEISAVN 370 380 390 400 410 420 DEAKLVLERM VYKLAYTFEV QKAAKEKNVG LDYNGVILFS GIHYLETNQH LVKEMNITDA 430 440 450 460 470 480 IYLYLRFTTP SLYSKVYYNV AVESVSRYWL WYAITTEPLE DVKKELKNLS VFVTKTLLHV 490 500 510 520 530 540 LLQKNCIQVN QQLRMITFTL LTRLLCLIPE KVAFEFILDV LKTSPLPLAK TSVLCVFKDL 550 560 570 580 590 600 SRRRISTKDN DSETDLIVEK LSKLKVNDSN KAQQSNIRHY IQLDSSKMKA VHDCCLQTIQ 610 620 630 640 650 660 DSFTADAKKS DILLLLTYLN IFIVLKKTWD EDLLKIVCSK IDSNLKSVEP DKLPKYKEIV 670 DKNESLNDYF TGIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A comprehensive two-hybrid analysis to explore the yeast protein interactome." Ito T., Chiba T., Ozawa R., Yoshida M., Hattori M., Sakaki Y. Proc. Natl. Acad. Sci. U.S.A. 98:4569-4574(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH NUP116.
[4]	"Ybp1 is required for the hydrogen peroxide-induced oxidation of the Yap1 transcription factor." Veal E.A., Ross S.J., Malakasi P., Peacock E., Morgan B.A. J. Biol. Chem. 278:30896-30904(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, OXIDATIVE STRESS RESPONSE.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z36085 Genomic DNA. Translation: CAA85180.1. BK006936 Genomic DNA. Translation: DAA07332.1.
PIR	S46092.
RefSeq	NP_009775.3. NM_001178564.3.
3D structure databases
ProteinModelPortal	P38315.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4499N.
IntAct	P38315. 17 interactions.
MINT	MINT-505614.
STRING	4932.YBR216C.
Proteomic databases
PaxDb	P38315.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR216C; YBR216C; YBR216C.
GeneID	852517.
KEGG	sce:YBR216C. sce:YBR220C.
Organism-specific databases
CYGD	YBR216c.
SGD	S000000420. YBP1.
Phylogenomic databases
eggNOG	NOG47496.
GeneTree	ENSGT00530000068379.
HOGENOM	HOG000141987.
OMA	YEIGWDL.
OrthoDB	EOG4RR9SB.
Gene expression databases
Genevestigator	P38315.
GermOnline	YBR216C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013877. YAP-bd/Alf4/glomulin. [Graphical view]
Pfam	PF08568. Kinetochor_Ybp2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971547.

Entry information

Entry name

YBP1_YEAST

Accession

Primary (citable) accession number: P38315
Secondary accession number(s): D6VQL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program