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P38315 (YBP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
YAP1-binding protein 1
Alternative name(s):
Activator of YAP1
Gene names
Name:YBP1
Ordered Locus Names:YBR216C
ORF Names:YBR1505
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length674 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in oxidative stress response and redox homeostasis. Required for hydrogen peroxide-induced oxidation and nuclear localization (activation) of YAP1. Functions probably in concert with HYP1/GPX3, the actual YAP1 modifying enzyme. YBP1 is not required for HYP1/GPX3-independent, diamide-induced oxidation of YAP1. Ref.3 Ref.4

Subunit structure

Interacts with YAP1. Forms a peroxide stress induced complex with YAP1 in the cytoplasm. Systematic proteome-wide 2-hybrid interaction studies suggest that YAP1, HYR1/GPX3, and YBP1 all interact with the nuclear pore complex subunit NUP116, which is involved in nucleocytoplasmic transport. Ref.3

Subcellular location

Cytoplasm Ref.5.

Miscellaneous

Present with 3100 molecules/cell in log phase SD medium.

Sequence similarities

To yeast YBP2.

Caution

YBP1 encoded by the widely used laboratory strain W303-1a is only partially functional, probably due to four amino acid substitutions.

Ontologies

Keywords
   Cellular componentCytoplasm
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to oxidative stress

Inferred from direct assay Ref.4. Source: SGD

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction Ref.3PubMed 11805826PubMed 16429126PubMed 18467557. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

YAP1P198804EBI-20985,EBI-31265

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 674674YAP1-binding protein 1
PRO_0000066150

Regions

Compositional bias613 – 6164Poly-Leu

Natural variations

Natural variant71I → L in strain: W303-1a.
Natural variant3281F → V in strain: W303-1a.
Natural variant3431K → E in strain: W303-1a.
Natural variant5701N → D in strain: W303-1a.

Sequences

Sequence LengthMass (Da)Tools
P38315 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 31F2B200CE34B32D

FASTA67477,741
        10         20         30         40         50         60 
MEPIDDILFE VTDAFKTQKE DLLELVTLID IYGEQVNQEG SYEEKTRFIE TLNTLLEDNP 

        70         80         90        100        110        120 
STTGEIGWDL PKGLLKFLSK DNVDVNGRLG TNMIVQGVMK CFYAISIQGE PKKCLITGLE 

       130        140        150        160        170        180 
LLSSLCSKDF SKSDQQNKED FVDKKANTLP PEGVIENSSN RKDFPSYGES KSSNEFFLKL 

       190        200        210        220        230        240 
KSYILFEFIG ASLKRISTLF PSKYLGAAVS TIEKFVYSHA DTFEDALFLL RRVYTFCRNY 

       250        260        270        280        290        300 
IPPDPPKDIQ LNEDFTREMF DKVVEEESEL QVRLLRRLCT FGISTPIKTV TTNADVKYYC 

       310        320        330        340        350        360 
ALNQQKFELS AYYTEYLELF CRYYQMAFSL DVDIEGEFQN VIKECRIIYK SVPQEISAVN 

       370        380        390        400        410        420 
DEAKLVLERM VYKLAYTFEV QKAAKEKNVG LDYNGVILFS GIHYLETNQH LVKEMNITDA 

       430        440        450        460        470        480 
IYLYLRFTTP SLYSKVYYNV AVESVSRYWL WYAITTEPLE DVKKELKNLS VFVTKTLLHV 

       490        500        510        520        530        540 
LLQKNCIQVN QQLRMITFTL LTRLLCLIPE KVAFEFILDV LKTSPLPLAK TSVLCVFKDL 

       550        560        570        580        590        600 
SRRRISTKDN DSETDLIVEK LSKLKVNDSN KAQQSNIRHY IQLDSSKMKA VHDCCLQTIQ 

       610        620        630        640        650        660 
DSFTADAKKS DILLLLTYLN IFIVLKKTWD EDLLKIVCSK IDSNLKSVEP DKLPKYKEIV 

       670 
DKNESLNDYF TGIK 

« Hide

References

« Hide 'large scale' references
[1]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"A comprehensive two-hybrid analysis to explore the yeast protein interactome."
Ito T., Chiba T., Ozawa R., Yoshida M., Hattori M., Sakaki Y.
Proc. Natl. Acad. Sci. U.S.A. 98:4569-4574(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NUP116.
[4]"Ybp1 is required for the hydrogen peroxide-induced oxidation of the Yap1 transcription factor."
Veal E.A., Ross S.J., Malakasi P., Peacock E., Morgan B.A.
J. Biol. Chem. 278:30896-30904(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, OXIDATIVE STRESS RESPONSE.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z36085 Genomic DNA. Translation: CAA85180.1.
BK006936 Genomic DNA. Translation: DAA07332.1.
PIRS46092.
RefSeqNP_009775.3. NM_001178564.3.

3D structure databases

ProteinModelPortalP38315.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32913. 20 interactions.
DIPDIP-4499N.
IntActP38315. 17 interactions.
MINTMINT-505614.
STRING4932.YBR216C.

Proteomic databases

MaxQBP38315.
PaxDbP38315.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR216C; YBR216C; YBR216C.
GeneID852517.
KEGGsce:YBR216C.

Organism-specific databases

CYGDYBR216c.
SGDS000000420. YBP1.

Phylogenomic databases

eggNOGNOG47496.
GeneTreeENSGT00530000068379.
HOGENOMHOG000141987.
OMAYEIGWDL.
OrthoDBEOG7NKKTX.

Enzyme and pathway databases

BioCycYEAST:G3O-29153-MONOMER.

Gene expression databases

GenevestigatorP38315.

Family and domain databases

InterProIPR013877. YAP-bd/ALF4/Glomulin.
[Graphical view]
PfamPF08568. Kinetochor_Ybp2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio971547.

Entry information

Entry nameYBP1_YEAST
AccessionPrimary (citable) accession number: P38315
Secondary accession number(s): D6VQL2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 11, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD