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P38313

- AME1_YEAST

UniProt

P38313 - AME1_YEAST

Protein

Central kinetochore subunit AME1

Gene

AME1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.

    GO - Biological processi

    1. attachment of spindle microtubules to kinetochore Source: SGD
    2. meiotic nuclear division Source: UniProtKB-KW
    3. mitotic nuclear division Source: UniProtKB-KW
    4. protein localization to kinetochore Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Meiosis, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29148-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Central kinetochore subunit AME1
    Alternative name(s):
    Associated with microtubules and essential protein 1
    Gene namesi
    Name:AME1
    Synonyms:ARP100
    Ordered Locus Names:YBR211C
    ORF Names:YBR1458
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR211c.
    SGDiS000000415. AME1.

    Subcellular locationi

    Nucleus 1 Publication. Chromosomecentromerekinetochore 1 Publication
    Note: Associated with kinetochores.

    GO - Cellular componenti

    1. COMA complex Source: SGD
    2. kinetochore Source: SGD
    3. spindle pole body Source: SGD

    Keywords - Cellular componenti

    Centromere, Chromosome, Kinetochore, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 324324Central kinetochore subunit AME1PRO_0000064578Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei41 – 411Phosphoserine3 Publications
    Modified residuei45 – 451Phosphoserine2 Publications
    Modified residuei53 – 531Phosphoserine1 Publication
    Modified residuei59 – 591Phosphoserine1 Publication
    Modified residuei101 – 1011Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38313.
    PaxDbiP38313.

    Expressioni

    Gene expression databases

    GenevestigatoriP38313.

    Interactioni

    Subunit structurei

    Component of the heterotetrameric kinetochore subcomplex COMA, which consists of AME1, CTF19, MCM21 and OKP1. The COMA subcomplex is part of a larger central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1.

    Protein-protein interaction databases

    BioGridi32908. 101 interactions.
    DIPiDIP-1588N.
    IntActiP38313. 9 interactions.
    MINTiMINT-385691.
    STRINGi4932.YBR211C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38313.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili199 – 26365Sequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG39832.
    HOGENOMiHOG000033919.
    KOiK11556.
    OMAiELCYQLK.
    OrthoDBiEOG70PC7P.

    Sequencei

    Sequence statusi: Complete.

    P38313-1 [UniParc]FASTAAdd to Basket

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    MDRDTKLAFR LRGSHSRRTD DIDDDVIVFK TPNAVYREEN SPIQSPVQPI    50
    LSSPKLANSF EFPITTNNVN AQDRHEHGYQ PLDAEDYPMI DSENKSLISE 100
    SPQNVRNDED LTTRYNFDDI PIRQLSSSIT SVTTIDVLSS LFINLFENDL 150
    IPQALKDFNK SDDDQFRKLL YKLDLRLFQT ISDQMTRDLK DILDINVSNN 200
    ELCYQLKQVL ARKEDLNQQI ISVRNEIQEL KAGKDWHDLQ NEQAKLNDKV 250
    KLNKRLNDLT STLLGKYEGD RKIMSQDSED DSIRDDSNIL DIAHFVDLMD 300
    PYNGLLKKIN KINENLSNEL QPSL 324
    Length:324
    Mass (Da):37,462
    Last modified:October 1, 1994 - v1
    Checksum:iC53CB31A7B42F6D5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z36080 Genomic DNA. Translation: CAA85175.1.
    AY692799 Genomic DNA. Translation: AAT92818.1.
    BK006936 Genomic DNA. Translation: DAA07327.1.
    PIRiS46085.
    RefSeqiNP_009770.3. NM_001178559.3.

    Genome annotation databases

    EnsemblFungiiYBR211C; YBR211C; YBR211C.
    GeneIDi852512.
    KEGGisce:YBR211C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z36080 Genomic DNA. Translation: CAA85175.1 .
    AY692799 Genomic DNA. Translation: AAT92818.1 .
    BK006936 Genomic DNA. Translation: DAA07327.1 .
    PIRi S46085.
    RefSeqi NP_009770.3. NM_001178559.3.

    3D structure databases

    ProteinModelPortali P38313.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32908. 101 interactions.
    DIPi DIP-1588N.
    IntActi P38313. 9 interactions.
    MINTi MINT-385691.
    STRINGi 4932.YBR211C.

    Proteomic databases

    MaxQBi P38313.
    PaxDbi P38313.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR211C ; YBR211C ; YBR211C .
    GeneIDi 852512.
    KEGGi sce:YBR211C.

    Organism-specific databases

    CYGDi YBR211c.
    SGDi S000000415. AME1.

    Phylogenomic databases

    eggNOGi NOG39832.
    HOGENOMi HOG000033919.
    KOi K11556.
    OMAi ELCYQLK.
    OrthoDBi EOG70PC7P.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29148-MONOMER.

    Miscellaneous databases

    NextBioi 971532.

    Gene expression databases

    Genevestigatori P38313.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p."
      Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S., Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G.
      Cell 111:163-172(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF CTF19 COMPLEX.
    5. "Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes."
      De Wulf P., McAinsh A.D., Sorger P.K.
      Genes Dev. 17:2902-2921(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF COMA COMPLEX.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41 AND SER-45, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41; SER-59 AND SER-101, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41; SER-45 AND SER-53, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAME1_YEAST
    AccessioniPrimary (citable) accession number: P38313
    Secondary accession number(s): D6VQK7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1630 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3