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Protein

Inner kinetochore subunit AME1

Gene

AME1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore COMA complex, which connects centromere-associated proteins and the outer kinetochore. COMA interacts with other inner kinetochore proteins to form the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.1 Publication

Miscellaneous

Present with 1630 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • attachment of spindle microtubules to kinetochore Source: SGD
  • cell division Source: UniProtKB-KW
  • meiotic cell cycle Source: UniProtKB-KW
  • protein localization to kinetochore Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Meiosis, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-29148-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Inner kinetochore subunit AME1Curated
Alternative name(s):
Associated with microtubules and essential protein 1
CENP-U homolog1 Publication
Constitutive centromere-associated network protein AME1Curated
Gene namesi
Name:AME1
Synonyms:ARP100
Ordered Locus Names:YBR211C
ORF Names:YBR1458
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR211C
SGDiS000000415 AME1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000645781 – 324Inner kinetochore subunit AME1Add BLAST324

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei41PhosphoserineCombined sources1
Modified residuei45PhosphoserineCombined sources1
Modified residuei53PhosphoserineCombined sources1
Modified residuei59PhosphoserineCombined sources1
Modified residuei101PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38313
PaxDbiP38313
PRIDEiP38313

PTM databases

iPTMnetiP38313

Interactioni

Subunit structurei

Component of the heterotetrameric kinetochore subcomplex COMA, which consists of AME1, CTF19, MCM21 and OKP1 (PubMed:14633972). The COMA subcomplex is part of a larger constitutive centromere-associated network (CCAN) (also known as central kinetochore CTF19 complex in yeast), which is composed of at least AME1, CHL4, CNN1, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, MHF1, MHF2, MIF2, NKP1, NKP2, OKP1 and WIP1 (PubMed:12408861, PubMed:22561346). COMA binds the centromeric nucleosome-binding protein MIF2, and to the outer kinetochore MIND subcomplex, probably via AME1. AME1 interacts directly with OKP1 and an NKP1-NKP2 dimer (By similarity).By similarity3 Publications

Protein-protein interaction databases

BioGridi32908, 286 interactors
DIPiDIP-1588N
IntActiP38313, 9 interactors
MINTiP38313
STRINGi4932.YBR211C

Structurei

3D structure databases

ProteinModelPortaliP38313
SMRiP38313
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili199 – 263Sequence analysisAdd BLAST65

Sequence similaritiesi

Belongs to the CENP-U/AME1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000033919
InParanoidiP38313
KOiK11556
OMAiELCYQLK
OrthoDBiEOG092C36MJ

Sequencei

Sequence statusi: Complete.

P38313-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRDTKLAFR LRGSHSRRTD DIDDDVIVFK TPNAVYREEN SPIQSPVQPI
60 70 80 90 100
LSSPKLANSF EFPITTNNVN AQDRHEHGYQ PLDAEDYPMI DSENKSLISE
110 120 130 140 150
SPQNVRNDED LTTRYNFDDI PIRQLSSSIT SVTTIDVLSS LFINLFENDL
160 170 180 190 200
IPQALKDFNK SDDDQFRKLL YKLDLRLFQT ISDQMTRDLK DILDINVSNN
210 220 230 240 250
ELCYQLKQVL ARKEDLNQQI ISVRNEIQEL KAGKDWHDLQ NEQAKLNDKV
260 270 280 290 300
KLNKRLNDLT STLLGKYEGD RKIMSQDSED DSIRDDSNIL DIAHFVDLMD
310 320
PYNGLLKKIN KINENLSNEL QPSL
Length:324
Mass (Da):37,462
Last modified:October 1, 1994 - v1
Checksum:iC53CB31A7B42F6D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36080 Genomic DNA Translation: CAA85175.1
AY692799 Genomic DNA Translation: AAT92818.1
BK006936 Genomic DNA Translation: DAA07327.1
PIRiS46085
RefSeqiNP_009770.3, NM_001178559.3

Genome annotation databases

EnsemblFungiiYBR211C; YBR211C; YBR211C
GeneIDi852512
KEGGisce:YBR211C

Similar proteinsi

Entry informationi

Entry nameiCENPU_YEAST
AccessioniPrimary (citable) accession number: P38313
Secondary accession number(s): D6VQK7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health