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Protein

Central kinetochore subunit AME1

Gene

AME1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.

GO - Biological processi

  • attachment of spindle microtubules to kinetochore Source: SGD
  • cell division Source: UniProtKB-KW
  • meiotic cell cycle Source: UniProtKB-KW
  • mitotic nuclear division Source: UniProtKB-KW
  • protein localization to kinetochore Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-29148-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Central kinetochore subunit AME1
Alternative name(s):
Associated with microtubules and essential protein 1
Gene namesi
Name:AME1
Synonyms:ARP100
Ordered Locus Names:YBR211C
ORF Names:YBR1458
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR211C.
SGDiS000000415. AME1.

Subcellular locationi

GO - Cellular componenti

  • COMA complex Source: SGD
  • kinetochore Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000645781 – 324Central kinetochore subunit AME1Add BLAST324

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei41PhosphoserineCombined sources1
Modified residuei45PhosphoserineCombined sources1
Modified residuei53PhosphoserineCombined sources1
Modified residuei59PhosphoserineCombined sources1
Modified residuei101PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38313.
PRIDEiP38313.

PTM databases

iPTMnetiP38313.

Interactioni

Subunit structurei

Component of the heterotetrameric kinetochore subcomplex COMA, which consists of AME1, CTF19, MCM21 and OKP1. The COMA subcomplex is part of a larger central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1.

Protein-protein interaction databases

BioGridi32908. 103 interactors.
DIPiDIP-1588N.
IntActiP38313. 9 interactors.
MINTiMINT-385691.

Structurei

3D structure databases

ProteinModelPortaliP38313.
SMRiP38313.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili199 – 263Sequence analysisAdd BLAST65

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000033919.
InParanoidiP38313.
KOiK11556.
OMAiELCYQLK.
OrthoDBiEOG092C36MJ.

Sequencei

Sequence statusi: Complete.

P38313-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRDTKLAFR LRGSHSRRTD DIDDDVIVFK TPNAVYREEN SPIQSPVQPI
60 70 80 90 100
LSSPKLANSF EFPITTNNVN AQDRHEHGYQ PLDAEDYPMI DSENKSLISE
110 120 130 140 150
SPQNVRNDED LTTRYNFDDI PIRQLSSSIT SVTTIDVLSS LFINLFENDL
160 170 180 190 200
IPQALKDFNK SDDDQFRKLL YKLDLRLFQT ISDQMTRDLK DILDINVSNN
210 220 230 240 250
ELCYQLKQVL ARKEDLNQQI ISVRNEIQEL KAGKDWHDLQ NEQAKLNDKV
260 270 280 290 300
KLNKRLNDLT STLLGKYEGD RKIMSQDSED DSIRDDSNIL DIAHFVDLMD
310 320
PYNGLLKKIN KINENLSNEL QPSL
Length:324
Mass (Da):37,462
Last modified:October 1, 1994 - v1
Checksum:iC53CB31A7B42F6D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36080 Genomic DNA. Translation: CAA85175.1.
AY692799 Genomic DNA. Translation: AAT92818.1.
BK006936 Genomic DNA. Translation: DAA07327.1.
PIRiS46085.
RefSeqiNP_009770.3. NM_001178559.3.

Genome annotation databases

EnsemblFungiiYBR211C; YBR211C; YBR211C.
GeneIDi852512.
KEGGisce:YBR211C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36080 Genomic DNA. Translation: CAA85175.1.
AY692799 Genomic DNA. Translation: AAT92818.1.
BK006936 Genomic DNA. Translation: DAA07327.1.
PIRiS46085.
RefSeqiNP_009770.3. NM_001178559.3.

3D structure databases

ProteinModelPortaliP38313.
SMRiP38313.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32908. 103 interactors.
DIPiDIP-1588N.
IntActiP38313. 9 interactors.
MINTiMINT-385691.

PTM databases

iPTMnetiP38313.

Proteomic databases

MaxQBiP38313.
PRIDEiP38313.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR211C; YBR211C; YBR211C.
GeneIDi852512.
KEGGisce:YBR211C.

Organism-specific databases

EuPathDBiFungiDB:YBR211C.
SGDiS000000415. AME1.

Phylogenomic databases

HOGENOMiHOG000033919.
InParanoidiP38313.
KOiK11556.
OMAiELCYQLK.
OrthoDBiEOG092C36MJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-29148-MONOMER.

Miscellaneous databases

PROiP38313.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiAME1_YEAST
AccessioniPrimary (citable) accession number: P38313
Secondary accession number(s): D6VQK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1630 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.