Iron transporter FTH1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38310 (FTH1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Iron transporter FTH1

Gene names

Name:	FTH1
Ordered Locus Names:	YBR207W
ORF Names:	YBR1446

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	High affinity iron transporter probably involved in transport of intravacuolar stores of iron. Ref.1
Subunit structure	Interacts with FET5. Ref.1
Subcellular location	Vacuole membrane; Multi-pass membrane protein Ref.1 Ref.5.
Miscellaneous	Present with 486 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Iron transport Transport
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix
Ligand	Iron
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	endocytosis Inferred from mutant phenotype. Source: SGD high-affinity iron ion transport Inferred from physical interaction Ref.1. Source: SGD iron ion homeostasis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	fungal-type vacuole membrane Inferred from direct assay Ref.1 Ref.5. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion transmembrane transporter activity Traceable author statement Ref.1. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FET5	P43561	7	EBI-20959,EBI-6891

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 465

465

Iron transporter FTH1

PRO_0000159650

Regions

Topological domain

1 – 11

Vacuolar Potential

Transmembrane

12 – 32

Helical; Potential

Topological domain

33 – 135

103

Cytoplasmic Potential

Transmembrane

136 – 156

Helical; Potential

Topological domain

157 – 170

Vacuolar Potential

Transmembrane

171 – 191

Helical; Potential

Topological domain

192 – 289

Cytoplasmic Potential

Transmembrane

290 – 310

Helical; Potential

Topological domain

311 – 358

Vacuolar Potential

Transmembrane

359 – 379

Helical; Potential

Topological domain

380 – 465

Cytoplasmic Potential

Amino acid modifications

Modified residue

285

Phosphoserine Ref.9

Modified residue

287

Phosphoserine Ref.9

Modified residue

449

Phosphoserine Ref.8 Ref.10

Modified residue

453

Phosphoserine Ref.10

Experimental info

Sequence conflict

E → K in CAA79694. Ref.2

Sequence conflict

E → K in CAA85171. Ref.3

Sequence conflict

G → D in CAA79694. Ref.2

Sequence conflict

G → D in CAA85171. Ref.3

Sequence conflict

228

E → Q in CAA79694. Ref.2

Sequence conflict

228

E → Q in CAA85171. Ref.3

Sequence conflict

249 – 250

VF → YS in CAA79694. Ref.2

Sequence conflict

249 – 250

VF → YS in CAA85171. Ref.3

Sequence conflict

334

G → E in CAA79694. Ref.2

Sequence conflict

334

G → E in CAA85171. Ref.3

Sequence conflict

389 – 390

IC → KY in CAA79694. Ref.2

Sequence conflict

389 – 390

IC → KY in CAA85171. Ref.3

Sequence conflict

399 – 401

EKY → GKC in CAA79694. Ref.2

Sequence conflict

399 – 401

EKY → GKC in CAA85171. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

P38310 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 7B4E620ABC39A053
Show »

FASTA

465

51,420

        10         20         30         40         50         60 
MAFEDYFSFQ IFFIFLRESL EIVVIVSILL TIVKQGLSVE DDSPFEGSSS SAGLPSPNTN 

        70         80         90        100        110        120 
TNADSTTAFL QAGPSDGNAI GTSATAANNK SRPLNVEEEE EIYEYSNELR DQDRESDEHT 

       130        140        150        160        170        180 
ADNVKLYQKL KIQILAGGAF GLLLCMLIGG AFVSIFYHIG TDLWTLSEHY YEGVLSLVAS 

       190        200        210        220        230        240 
VIISVMGLFF LRMGKLREKF RVKLASIIYS KDNNLLGNKT QKGVKFSEKY SFFILPFITT 

       250        260        270        280        290        300 
LREGLEAVVF IGGIGIDQPL SSIPLSMVLA TAISTVFGIF FFRYSSSLSL KICLVVATCF 

       310        320        330        340        350        360 
LYLIAAGLFS KGVWQLELQD YVNKCNGQDM SEVGNGPGSY DISRSVWHVN CCNGEKDGGW 

       370        380        390        400        410        420 
MIFTAIFGWT NSATVGSVIS YNAYWLVLIC ALKLLMIEEK YGYIPYLPIS WQKKRIMKRL 

       430        440        450        460 
SIAKASLDLK HHTSELNSST SEPDSQRRSK DSSVPLIIDS SGSAN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The iron transporter Fth1p forms a complex with the Fet5 iron oxidase and resides on the vacuolar membrane." Urbanowski J.L., Piper R.C. J. Biol. Chem. 274:38061-38070(1999) [PubMed: 10608875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH FET5. Strain: ATCC 96099 / S288c / SEY6210.
[2]	"A 12.8 kb segment, on the right arm of chromosome II from Saccharomyces cerevisiae including part of the DUR1,2 gene, contains five putative new genes." Bussereau F., Mallet L., Gaillon L., Jacquet M. Yeast 9:797-806(1993) [PubMed: 8368014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 18; 36; 228; 249-250; 334; 389-390 AND 399-401. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-449, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-285 AND SER-287, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-449 AND SER-453, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF177330 Genomic DNA. Translation: AAD53168.1. Z21487 Genomic DNA. Translation: CAA79694.1. Z36076 Genomic DNA. Translation: CAA85171.1. BK006936 Genomic DNA. Translation: DAA07324.2.
PIR	S34929.
RefSeq	NP_009766.2. NM_001178555.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4165N.
IntAct	P38310. 50 interactions.
MINT	MINT-532026.
STRING	P38310.
Protein family/group databases
TCDB	9.A.10.1.4. iron/lead transporter (ILT) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	852506.
KEGG	sce:YBR207W.
NMPDR	fig\|4932.3.peg.477.
Organism-specific databases
CYGD	YBR207w.
SGD	S000000411. FTH1.
Phylogenomic databases
eggNOG	fuNOG05641.
GeneTree	EFGT00050000006630.
HOGENOM	HBG325002.
OMA	SEHYYEG.
OrthoDB	EOG479JGX.
Gene expression databases
ArrayExpress	P38310.
Genevestigator	P38310.
GermOnline	YBR207W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004923. FTR1. [Graphical view]
Pfam	PF03239. FTR1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971519.

Entry information

Entry name

FTH1_YEAST

Accession

Primary (citable) accession number: P38310
Secondary accession number(s): D6VQK4, Q9URQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	July 27, 2011
Last modified:	January 25, 2012
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents