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P38304

- MED8_YEAST

UniProt

P38304 - MED8_YEAST

Protein

Mediator of RNA polymerase II transcription subunit 8

Gene

MED8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. MED8 binds to the consensus sequence 5'-[AC][AG]GAAAT-3' in both the UAS of SUC2 and the DRS2 of HXK2.6 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
    3. RNA polymerase II transcription corepressor activity Source: SGD
    4. structural molecule activity Source: SGD
    5. TBP-class protein binding Source: SGD

    GO - Biological processi

    1. negative regulation of transcription from RNA polymerase II promoter Source: SGD
    2. positive regulation of transcription from RNA polymerase II promoter Source: SGD

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29135-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 8
    Alternative name(s):
    Mediator complex subunit 8
    Gene namesi
    Name:MED8
    Ordered Locus Names:YBR193C
    ORF Names:YBR1403
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    SGDiS000000397. MED8.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. core mediator complex Source: SGD
    2. mediator complex Source: InterPro

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 223222Mediator of RNA polymerase II transcription subunit 8PRO_0000096397Add
    BLAST

    Proteomic databases

    MaxQBiP38304.
    PaxDbiP38304.
    PeptideAtlasiP38304.

    Expressioni

    Gene expression databases

    GenevestigatoriP38304.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. MED8 interacts directly with SRB5/MED18. Interacts also with Hexokinase B (HXK2). Interacts with TBP1.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SOH1P386333EBI-20932,EBI-17658
    SRB5P325853EBI-20932,EBI-18032

    Protein-protein interaction databases

    BioGridi32890. 174 interactions.
    DIPiDIP-1659N.
    IntActiP38304. 14 interactions.
    MINTiMINT-383805.
    STRINGi4932.YBR193C.

    Structurei

    Secondary structure

    1
    223
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi198 – 20710

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2HZSX-ray2.70I/J/K/L190-210[»]
    3J1Oelectron microscopy16.00J1-223[»]
    3RJ1X-ray4.30C/J/Q1-223[»]
    4GWPX-ray4.20C1-223[»]
    4GWQX-ray4.50C1-223[»]
    ProteinModelPortaliP38304.
    SMRiP38304. Positions 23-214.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38304.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 138137Interaction with TBP1Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili33 – 5927Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 8 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG75044.
    HOGENOMiHOG000111183.
    KOiK15130.
    OMAiFPTTAHE.
    OrthoDBiEOG70W3RN.

    Family and domain databases

    InterProiIPR020178. Mediatior_Med8_fun.
    IPR019364. Mediatior_Med8_fun/met.
    [Graphical view]
    PfamiPF10232. Med8. 1 hit.
    [Graphical view]
    ProDomiPD114219. Mediatior_Med8_fun. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38304-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQSTASLVP EGNQGSLQED VSFDFNGVPG QALDAVRMRL AQLTHSLRRI    50
    RDEMSKAELP QWYTLQSQLN VTLSQLVSVT STLQHFQETL DSTVVYPLPK 100
    FPTTSHESLV TTLLRKKNIP EVDEWMKYVR ETSGVTTALL KDEEIEKLLQ 150
    QDREITNWAR TTFRNEYGKH DFKNEESLSE EHASLLVRDS KPSKPFNVDD 200
    VLKFTFTGEK PIITGSTSTS SSN 223
    Length:223
    Mass (Da):25,268
    Last modified:January 23, 2007 - v3
    Checksum:i86B2A064293C6B61
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z21487 Genomic DNA. Translation: CAA79680.1.
    Z36062 Genomic DNA. Translation: CAA85155.1.
    AY557711 Genomic DNA. Translation: AAS56037.1.
    BK006936 Genomic DNA. Translation: DAA07307.1.
    PIRiS34018.
    RefSeqiNP_009752.3. NM_001178541.3.

    Genome annotation databases

    EnsemblFungiiYBR193C; YBR193C; YBR193C.
    GeneIDi852492.
    KEGGisce:YBR193C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z21487 Genomic DNA. Translation: CAA79680.1 .
    Z36062 Genomic DNA. Translation: CAA85155.1 .
    AY557711 Genomic DNA. Translation: AAS56037.1 .
    BK006936 Genomic DNA. Translation: DAA07307.1 .
    PIRi S34018.
    RefSeqi NP_009752.3. NM_001178541.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2HZS X-ray 2.70 I/J/K/L 190-210 [» ]
    3J1O electron microscopy 16.00 J 1-223 [» ]
    3RJ1 X-ray 4.30 C/J/Q 1-223 [» ]
    4GWP X-ray 4.20 C 1-223 [» ]
    4GWQ X-ray 4.50 C 1-223 [» ]
    ProteinModelPortali P38304.
    SMRi P38304. Positions 23-214.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32890. 174 interactions.
    DIPi DIP-1659N.
    IntActi P38304. 14 interactions.
    MINTi MINT-383805.
    STRINGi 4932.YBR193C.

    Proteomic databases

    MaxQBi P38304.
    PaxDbi P38304.
    PeptideAtlasi P38304.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR193C ; YBR193C ; YBR193C .
    GeneIDi 852492.
    KEGGi sce:YBR193C.

    Organism-specific databases

    SGDi S000000397. MED8.

    Phylogenomic databases

    eggNOGi NOG75044.
    HOGENOMi HOG000111183.
    KOi K15130.
    OMAi FPTTAHE.
    OrthoDBi EOG70W3RN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29135-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P38304.
    NextBioi 971480.

    Gene expression databases

    Genevestigatori P38304.

    Family and domain databases

    InterProi IPR020178. Mediatior_Med8_fun.
    IPR019364. Mediatior_Med8_fun/met.
    [Graphical view ]
    Pfami PF10232. Med8. 1 hit.
    [Graphical view ]
    ProDomi PD114219. Mediatior_Med8_fun. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "RIM2, MSI1 and PGI1 are located within an 8 kb segment of Saccharomyces cerevisiae chromosome II, which also contains the putative ribosomal gene L21 and a new putative essential gene with a leucine zipper motif."
      Demolis N., Mallet L., Bussereau F., Jacquet M.
      Yeast 9:645-659(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Med8, a subunit of the mediator CTD complex of RNA polymerase II, directly binds to regulatory elements of SUC2 and HXK2 genes."
      Chaves R.S., Herrero P., Moreno F.
      Biochem. Biophys. Res. Commun. 254:345-350(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-11, FUNCTION.
    6. "The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain."
      Myers L.C., Gustafsson C.M., Bushnell D.A., Lui M., Erdjument-Bromage H., Tempst P., Kornberg R.D.
      Genes Dev. 12:45-54(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF MEDIATOR COMPLEX.
    7. "Analysis by atomic force microscopy of Med8 binding to cis-acting regulatory elements of the SUC2 and HXK2 genes of Saccharomyces cerevisiae."
      Moreno-Herrero F., Herrero P., Colchero J., Baro A.M., Moreno F.
      FEBS Lett. 459:427-432(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING.
    8. "The structural and functional organization of the yeast mediator complex."
      Kang J.S., Kim S.H., Hwang M.S., Han S.J., Lee Y.C., Kim Y.-J.
      J. Biol. Chem. 276:42003-42010(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SRB5, FUNCTION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    9. "Mediator factor Med8p interacts with the hexokinase 2: implication in the glucose signalling pathway of Saccharomyces cerevisiae."
      de la Cera T., Herrero P., Moreno-Herrero F., Chaves R.S., Moreno F.
      J. Mol. Biol. 319:703-714(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HXK2.
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. Cited for: NOMENCLATURE.
    12. Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
    13. "Preponderance of free mediator in the yeast Saccharomyces cerevisiae."
      Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J.
      J. Biol. Chem. 280:31200-31207(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
    14. "Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
      Nair D., Kim Y., Myers L.C.
      J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
    15. "Mediator as a general transcription factor."
      Takagi Y., Kornberg R.D.
      J. Biol. Chem. 281:80-89(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
    16. Cited for: INTERACTION WITH SRB4, FUNCTION OF THE MEDIATOR COMPLEX HEAD MODULE, ELECTRON MICROSCOPY OF THE MEDIATOR COMPLEX HEAD MODULE, INTERACTION OF THE MEDIATOR COMPLEX HEAD MODULE WITH RNA POLYMERASE II AND TFIIF.
    17. "Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex."
      Baidoobonso S.M., Guidi B.W., Myers L.C.
      J. Biol. Chem. 282:5551-5559(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SRB5, CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    18. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    19. "Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction."
      Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J.
      Mol. Cell 10:409-415(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.
    20. "Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20."
      Lariviere L., Geiger S., Hoeppner S., Roether S., Straesser K., Cramer P.
      Nat. Struct. Mol. Biol. 13:895-901(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY STRUCTURE (2.7 ANGSTROMS) OF 190-210 IN COMPLEX WITH SRB2 AND SRB5, INTERACTION WITH TBP1.

    Entry informationi

    Entry nameiMED8_YEAST
    AccessioniPrimary (citable) accession number: P38304
    Secondary accession number(s): D6VQI7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 120 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 937 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3