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P38304 (MED8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mediator of RNA polymerase II transcription subunit 8
Alternative name(s):
Mediator complex subunit 8
Gene names
Name:MED8
Ordered Locus Names:YBR193C
ORF Names:YBR1403
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length223 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. MED8 binds to the consensus sequence 5'-[AC][AG]GAAAT-3' in both the UAS of SUC2 and the DRS2 of HXK2. Ref.5 Ref.7 Ref.8 Ref.14 Ref.15 Ref.16

Subunit structure

Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. MED8 interacts directly with SRB5/MED18. Interacts also with Hexokinase B (HXK2). Interacts with TBP1. Ref.8 Ref.9 Ref.16 Ref.17 Ref.20

Subcellular location

Nucleus Probable.

Miscellaneous

Present with 937 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the Mediator complex subunit 8 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SOH1P386333EBI-20932,EBI-17658
SRB5P325853EBI-20932,EBI-18032

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 223222Mediator of RNA polymerase II transcription subunit 8
PRO_0000096397

Regions

Region2 – 138137Interaction with TBP1
Coiled coil33 – 5927 Potential

Secondary structure

... 223
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P38304 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 86B2A064293C6B61

FASTA22325,268
        10         20         30         40         50         60 
MSQSTASLVP EGNQGSLQED VSFDFNGVPG QALDAVRMRL AQLTHSLRRI RDEMSKAELP 

        70         80         90        100        110        120 
QWYTLQSQLN VTLSQLVSVT STLQHFQETL DSTVVYPLPK FPTTSHESLV TTLLRKKNIP 

       130        140        150        160        170        180 
EVDEWMKYVR ETSGVTTALL KDEEIEKLLQ QDREITNWAR TTFRNEYGKH DFKNEESLSE 

       190        200        210        220 
EHASLLVRDS KPSKPFNVDD VLKFTFTGEK PIITGSTSTS SSN 

« Hide

References

« Hide 'large scale' references
[1]"RIM2, MSI1 and PGI1 are located within an 8 kb segment of Saccharomyces cerevisiae chromosome II, which also contains the putative ribosomal gene L21 and a new putative essential gene with a leucine zipper motif."
Demolis N., Mallet L., Bussereau F., Jacquet M.
Yeast 9:645-659(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Med8, a subunit of the mediator CTD complex of RNA polymerase II, directly binds to regulatory elements of SUC2 and HXK2 genes."
Chaves R.S., Herrero P., Moreno F.
Biochem. Biophys. Res. Commun. 254:345-350(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-11, FUNCTION.
[6]"The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain."
Myers L.C., Gustafsson C.M., Bushnell D.A., Lui M., Erdjument-Bromage H., Tempst P., Kornberg R.D.
Genes Dev. 12:45-54(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF MEDIATOR COMPLEX.
[7]"Analysis by atomic force microscopy of Med8 binding to cis-acting regulatory elements of the SUC2 and HXK2 genes of Saccharomyces cerevisiae."
Moreno-Herrero F., Herrero P., Colchero J., Baro A.M., Moreno F.
FEBS Lett. 459:427-432(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING.
[8]"The structural and functional organization of the yeast mediator complex."
Kang J.S., Kim S.H., Hwang M.S., Han S.J., Lee Y.C., Kim Y.-J.
J. Biol. Chem. 276:42003-42010(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SRB5, FUNCTION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[9]"Mediator factor Med8p interacts with the hexokinase 2: implication in the glucose signalling pathway of Saccharomyces cerevisiae."
de la Cera T., Herrero P., Moreno-Herrero F., Chaves R.S., Moreno F.
J. Mol. Biol. 319:703-714(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HXK2.
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II."
Bourbon H.-M., Aguilera A., Ansari A.Z., Asturias F.J., Berk A.J., Bjoerklund S., Blackwell T.K., Borggrefe T., Carey M., Carlson M., Conaway J.W., Conaway R.C., Emmons S.W., Fondell J.D., Freedman L.P., Fukasawa T., Gustafsson C.M., Han M. expand/collapse author list , He X., Herman P.K., Hinnebusch A.G., Holmberg S., Holstege F.C.P., Jaehning J.A., Kim Y.-J., Kuras L., Leutz A., Lis J.T., Meisterernest M., Naeaer A.M., Nasmyth K., Parvin J.D., Ptashne M., Reinberg D., Ronne H., Sadowski I., Sakurai H., Sipiczki M., Sternberg P.W., Stillman D.J., Strich R., Struhl K., Svejstrup J.Q., Tuck S., Winston F., Roeder R.G., Kornberg R.D.
Mol. Cell 14:553-557(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
[12]"A high resolution protein interaction map of the yeast Mediator complex."
Guglielmi B., van Berkum N.L., Klapholz B., Bijma T., Boube M., Boschiero C., Bourbon H.-M., Holstege F.C.P., Werner M.
Nucleic Acids Res. 32:5379-5391(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
[13]"Preponderance of free mediator in the yeast Saccharomyces cerevisiae."
Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J.
J. Biol. Chem. 280:31200-31207(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
[14]"Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
Nair D., Kim Y., Myers L.C.
J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[15]"Mediator as a general transcription factor."
Takagi Y., Kornberg R.D.
J. Biol. Chem. 281:80-89(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[16]"Head module control of mediator interactions."
Takagi Y., Calero G., Komori H., Brown J.A., Ehrensberger A.H., Hudmon A., Asturias F.J., Kornberg R.D.
Mol. Cell 23:355-364(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SRB4, FUNCTION OF THE MEDIATOR COMPLEX HEAD MODULE, ELECTRON MICROSCOPY OF THE MEDIATOR COMPLEX HEAD MODULE, INTERACTION OF THE MEDIATOR COMPLEX HEAD MODULE WITH RNA POLYMERASE II AND TFIIF.
[17]"Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex."
Baidoobonso S.M., Guidi B.W., Myers L.C.
J. Biol. Chem. 282:5551-5559(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SRB5, CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[18]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[19]"Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction."
Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J.
Mol. Cell 10:409-415(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.
[20]"Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20."
Lariviere L., Geiger S., Hoeppner S., Roether S., Straesser K., Cramer P.
Nat. Struct. Mol. Biol. 13:895-901(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY STRUCTURE (2.7 ANGSTROMS) OF 190-210 IN COMPLEX WITH SRB2 AND SRB5, INTERACTION WITH TBP1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z21487 Genomic DNA. Translation: CAA79680.1.
Z36062 Genomic DNA. Translation: CAA85155.1.
AY557711 Genomic DNA. Translation: AAS56037.1.
BK006936 Genomic DNA. Translation: DAA07307.1.
PIRS34018.
RefSeqNP_009752.3. NM_001178541.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2HZSX-ray2.70I/J/K/L190-210[»]
3J1Oelectron microscopy16.00J1-223[»]
3RJ1X-ray4.30C/J/Q1-223[»]
4GWPX-ray4.20C1-223[»]
4GWQX-ray4.50C1-223[»]
ProteinModelPortalP38304.
SMRP38304. Positions 23-214.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32890. 174 interactions.
DIPDIP-1659N.
IntActP38304. 14 interactions.
MINTMINT-383805.
STRING4932.YBR193C.

Proteomic databases

MaxQBP38304.
PaxDbP38304.
PeptideAtlasP38304.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR193C; YBR193C; YBR193C.
GeneID852492.
KEGGsce:YBR193C.

Organism-specific databases

SGDS000000397. MED8.

Phylogenomic databases

eggNOGNOG75044.
HOGENOMHOG000111183.
KOK15130.
OMAFPTTAHE.
OrthoDBEOG70W3RN.

Enzyme and pathway databases

BioCycYEAST:G3O-29135-MONOMER.

Gene expression databases

GenevestigatorP38304.

Family and domain databases

InterProIPR020178. Mediatior_Med8_fun.
IPR019364. Mediatior_Med8_fun/met.
[Graphical view]
PfamPF10232. Med8. 1 hit.
[Graphical view]
ProDomPD114219. Mediatior_Med8_fun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Other

EvolutionaryTraceP38304.
NextBio971480.

Entry information

Entry nameMED8_YEAST
AccessionPrimary (citable) accession number: P38304
Secondary accession number(s): D6VQI7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references