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Protein

GCR1-dependent translation factor 1

Gene

GDT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in ribosome biogenesis. Has also been proposed to play a role in calcium homeostasis (PubMed:23569283).1 Publication

GO - Biological processi

  • cellular calcium ion homeostasis Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29130-MONOMER.

Protein family/group databases

TCDBi2.A.106.2.3. the ca(2+):h(+) antiporter-2 (caca2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
GCR1-dependent translation factor 1
Gene namesi
Name:GDT1
Ordered Locus Names:YBR187W
ORF Names:YBR1310
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR187W.
SGDiS000000391. GDT1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 33CytoplasmicSequence analysis
Transmembranei4 – 2421HelicalSequence analysisAdd
BLAST
Topological domaini25 – 4521VacuolarSequence analysisAdd
BLAST
Transmembranei46 – 6621HelicalSequence analysisAdd
BLAST
Topological domaini67 – 715CytoplasmicSequence analysis
Transmembranei72 – 9221HelicalSequence analysisAdd
BLAST
Topological domaini93 – 10412VacuolarSequence analysisAdd
BLAST
Transmembranei105 – 12521HelicalSequence analysisAdd
BLAST
Topological domaini126 – 18358CytoplasmicSequence analysisAdd
BLAST
Transmembranei184 – 20421HelicalSequence analysisAdd
BLAST
Topological domaini205 – 22218VacuolarSequence analysisAdd
BLAST
Transmembranei223 – 24321HelicalSequence analysisAdd
BLAST
Topological domaini244 – 25512CytoplasmicSequence analysisAdd
BLAST
Transmembranei256 – 27621HelicalSequence analysisAdd
BLAST
Topological domaini277 – 2804VacuolarSequence analysis

GO - Cellular componenti

  • cis-Golgi network Source: SGD
  • fungal-type vacuole Source: SGD
  • Golgi apparatus Source: UniProtKB
  • Golgi medial cisterna Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi71 – 711R → C or H: Loss of function in calcium homeostasis. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 280280GCR1-dependent translation factor 1PRO_0000212467Add
BLAST

Proteomic databases

MaxQBiP38301.

Expressioni

Inductioni

Expressin is GCR1-dependent. Promoter is also bound by the RAP1 transcrition factor.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi32885. 19 interactions.
DIPiDIP-3804N.
IntActiP38301. 2 interactions.
MINTiMINT-474384.

Structurei

3D structure databases

ProteinModelPortaliP38301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GDT1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000005261.
InParanoidiP38301.
OMAiAGSEYWF.
OrthoDBiEOG783N5X.

Family and domain databases

InterProiIPR001727. Gdt1.
[Graphical view]
PANTHERiPTHR12608. PTHR12608. 1 hit.
PfamiPF01169. UPF0016. 2 hits.
[Graphical view]
PROSITEiPS01214. UPF0016. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38301-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNMIKKASL IALLPLFTAA AAAATDAETS MESGSSSHLK SFLMSVSMIG
60 70 80 90 100
LSEIGDKTFL IAALMAMRHK RVLVFSAAAT SLAIMTILSG VVGHSAVAFL
110 120 130 140 150
SERYTAFFAG ILFLVFGYKL TMEGLEMSKD AGVEEEMAEV EEEIAIKDMN
160 170 180 190 200
QDMDDVEKGG DTAYDKQLKN ASIGKKIVHR IRELASFMFS PVWVQIFLMV
210 220 230 240 250
FLGELGDRSQ ISIIAMATDS DYWYVIAGAV IGHAICSGLA VVGGKLLATR
260 270 280
ISIRTITLAS SLLFFIFALM YIYQAFTTQD
Length:280
Mass (Da):30,341
Last modified:October 1, 1994 - v1
Checksum:i34CDAE95E13169FE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti158 – 1581K → E in AAS56893 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02073 Genomic DNA. Translation: AAB60282.1.
Z36056 Genomic DNA. Translation: CAA85148.1.
Z36057 Genomic DNA. Translation: CAA85150.1.
AY558567 Genomic DNA. Translation: AAS56893.1.
BK006936 Genomic DNA. Translation: DAA07302.1.
PIRiS46059.
RefSeqiNP_009746.1. NM_001178535.1.

Genome annotation databases

EnsemblFungiiYBR187W; YBR187W; YBR187W.
GeneIDi852485.
KEGGisce:YBR187W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02073 Genomic DNA. Translation: AAB60282.1.
Z36056 Genomic DNA. Translation: CAA85148.1.
Z36057 Genomic DNA. Translation: CAA85150.1.
AY558567 Genomic DNA. Translation: AAS56893.1.
BK006936 Genomic DNA. Translation: DAA07302.1.
PIRiS46059.
RefSeqiNP_009746.1. NM_001178535.1.

3D structure databases

ProteinModelPortaliP38301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32885. 19 interactions.
DIPiDIP-3804N.
IntActiP38301. 2 interactions.
MINTiMINT-474384.

Protein family/group databases

TCDBi2.A.106.2.3. the ca(2+):h(+) antiporter-2 (caca2) family.

Proteomic databases

MaxQBiP38301.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR187W; YBR187W; YBR187W.
GeneIDi852485.
KEGGisce:YBR187W.

Organism-specific databases

EuPathDBiFungiDB:YBR187W.
SGDiS000000391. GDT1.

Phylogenomic databases

GeneTreeiENSGT00390000005261.
InParanoidiP38301.
OMAiAGSEYWF.
OrthoDBiEOG783N5X.

Enzyme and pathway databases

BioCyciYEAST:G3O-29130-MONOMER.

Miscellaneous databases

PROiP38301.

Family and domain databases

InterProiIPR001727. Gdt1.
[Graphical view]
PANTHERiPTHR12608. PTHR12608. 1 hit.
PfamiPF01169. UPF0016. 2 hits.
[Graphical view]
PROSITEiPS01214. UPF0016. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A 12.5 kb fragment of the yeast chromosome II contains two adjacent genes encoding ribosomal proteins and six putative new genes, one of which encodes a putative transcriptional factor."
    Demolis N., Jacquet M., Mallet L.
    Yeast 10:1511-1525(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  8. "The transcription factor Gcr1 stimulates cell growth by participating in nutrient-responsive gene expression on a global level."
    Barbara K.E., Haley T.M., Willis K.A., Santangelo G.M.
    Mol. Genet. Genomics 277:171-188(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  9. "Impact of disease-causing mutations on TMEM165 subcellular localization, a recently identified protein involved in CDG-II."
    Rosnoblet C., Legrand D., Demaegd D., Hacine-Gherbi H., de Bettignies G., Bammens R., Borrego C., Duvet S., Morsomme P., Matthijs G., Foulquier F.
    Hum. Mol. Genet. 22:2914-2928(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ARG-71.
  10. "Newly characterized Golgi-localized family of proteins is involved in calcium and pH homeostasis in yeast and human cells."
    Demaegd D., Foulquier F., Colinet A.S., Gremillon L., Legrand D., Mariot P., Peiter E., Van Schaftingen E., Matthijs G., Morsomme P.
    Proc. Natl. Acad. Sci. U.S.A. 110:6859-6864(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CALCIUM HOMEOSTASIS, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGDT1_YEAST
AccessioniPrimary (citable) accession number: P38301
Secondary accession number(s): D6VQI2, E9PAD4, Q6Q545
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 6, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2170 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.