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P38298

- YPC1_YEAST

UniProt

P38298 - YPC1_YEAST

Protein

Alkaline ceramidase YPC1

Gene

YPC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Hydrolyzes phytoceramide and also dihydroceramide into phytosphingosine or dihydrosphingosine. Prefers phytoceramide. Has also reverse hydrolysis activity, catalyzing synthesis of phytoceramide and dihydroceramide from palmitic acid and phytosphingosine or dihydrosphingosine. Is not responsible for the breakdown of unsaturated ceramide.

    GO - Molecular functioni

    1. phytoceramidase activity Source: SGD
    2. sphingosine N-acyltransferase activity Source: SGD

    GO - Biological processi

    1. ceramide biosynthetic process Source: SGD
    2. ceramide catabolic process Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciMetaCyc:YBR183W-MONOMER.
    YEAST:YBR183W-MONOMER.
    ReactomeiREACT_189220. Sphingolipid de novo biosynthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline ceramidase YPC1 (EC:3.5.1.-)
    Gene namesi
    Name:YPC1
    Ordered Locus Names:YBR183W
    ORF Names:YBR1305
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR183w.
    SGDiS000000387. YPC1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. Golgi membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 316316Alkaline ceramidase YPC1PRO_0000212466Add
    BLAST

    Proteomic databases

    PaxDbiP38298.

    Expressioni

    Gene expression databases

    GenevestigatoriP38298.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AGP1P253761EBI-2103141,EBI-2357
    CHO1P084561EBI-2103141,EBI-14055
    ERV29P533371EBI-2103141,EBI-23662
    OST1P415431EBI-2103141,EBI-12651
    PMP2P409751EBI-2103141,EBI-2043041
    RTN1Q049471EBI-2103141,EBI-38020
    SAC1P323681EBI-2103141,EBI-16210
    SPF1P399861EBI-2103141,EBI-3128
    TAF2P232551EBI-2103141,EBI-18862
    YHR080CP388001EBI-2103141,EBI-24597

    Protein-protein interaction databases

    BioGridi32881. 67 interactions.
    DIPiDIP-7405N.
    IntActiP38298. 30 interactions.
    MINTiMINT-1354145.
    STRINGi4932.YBR183W.

    Structurei

    3D structure databases

    ProteinModelPortaliP38298.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6868CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini90 – 934LumenalSequence Analysis
    Topological domaini115 – 13521CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini157 – 1604LumenalSequence Analysis
    Topological domaini182 – 19514CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini217 – 22812LumenalSequence AnalysisAdd
    BLAST
    Topological domaini250 – 31667CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei69 – 8921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei94 – 11421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei136 – 15621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei161 – 18121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei196 – 21621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei229 – 24921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline ceramidase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG250726.
    GeneTreeiENSGT00730000111189.
    HOGENOMiHOG000192011.
    KOiK05848.
    OMAiCEAKEAL.
    OrthoDBiEOG76DV3Q.

    Family and domain databases

    InterProiIPR008901. Ceramidase.
    [Graphical view]
    PfamiPF05875. Ceramidase. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38298-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGIFRWNYPE SSVPGVWGET TSTIDWCEEN YVVSPYIAEW SNTLTNSVFI    50
    LSAIYTTYSA YKNKLEKRFL LIGFGYGLVG VGSWLFHMTL KYRFQLLDEL 100
    PMIYAMCIPT WSLVCEAKEA LLNGDNHKKV PLFEQIFIGV IIGLAVTTAS 150
    ILYVIYKNVD IHQILFGVQI VVVAATAGSL TYRYVHDPLA KRNLKASMAL 200
    GAILFLSGYI SWLLDIHYCS FWVHVRRSIL ALPLGVLLEP HGWWHILTGM 250
    GIYFYIVSLE HLRVITLNVS CNYQFIWRWK VFPELIWKGR KPSTRYSLEL 300
    FGPYVEDQSI EVKKEK 316
    Length:316
    Mass (Da):36,420
    Last modified:October 1, 1994 - v1
    Checksum:i90DD942A4522508F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF191745 Genomic DNA. Translation: AAF43604.1.
    U02073 Genomic DNA. Translation: AAB60277.1.
    Z36052 Genomic DNA. Translation: CAA85144.1.
    BK006936 Genomic DNA. Translation: DAA07298.1.
    PIRiS46055.
    RefSeqiNP_009742.1. NM_001178531.1.

    Genome annotation databases

    EnsemblFungiiYBR183W; YBR183W; YBR183W.
    GeneIDi852481.
    KEGGisce:YBR183W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF191745 Genomic DNA. Translation: AAF43604.1 .
    U02073 Genomic DNA. Translation: AAB60277.1 .
    Z36052 Genomic DNA. Translation: CAA85144.1 .
    BK006936 Genomic DNA. Translation: DAA07298.1 .
    PIRi S46055.
    RefSeqi NP_009742.1. NM_001178531.1.

    3D structure databases

    ProteinModelPortali P38298.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32881. 67 interactions.
    DIPi DIP-7405N.
    IntActi P38298. 30 interactions.
    MINTi MINT-1354145.
    STRINGi 4932.YBR183W.

    Proteomic databases

    PaxDbi P38298.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR183W ; YBR183W ; YBR183W .
    GeneIDi 852481.
    KEGGi sce:YBR183W.

    Organism-specific databases

    CYGDi YBR183w.
    SGDi S000000387. YPC1.

    Phylogenomic databases

    eggNOGi NOG250726.
    GeneTreei ENSGT00730000111189.
    HOGENOMi HOG000192011.
    KOi K05848.
    OMAi CEAKEAL.
    OrthoDBi EOG76DV3Q.

    Enzyme and pathway databases

    BioCyci MetaCyc:YBR183W-MONOMER.
    YEAST:YBR183W-MONOMER.
    Reactomei REACT_189220. Sphingolipid de novo biosynthesis.

    Miscellaneous databases

    NextBioi 971453.
    PROi P38298.

    Gene expression databases

    Genevestigatori P38298.

    Family and domain databases

    InterProi IPR008901. Ceramidase.
    [Graphical view ]
    Pfami PF05875. Ceramidase. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of an alkaline ceramidase from Saccharomyces cerevisiae. An enzyme with reverse (CoA-independent) ceramide synthase activity."
      Mao C., Xu R., Bielawska A., Obeid L.M.
      J. Biol. Chem. 275:6876-6884(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    2. "A 12.5 kb fragment of the yeast chromosome II contains two adjacent genes encoding ribosomal proteins and six putative new genes, one of which encodes a putative transcriptional factor."
      Demolis N., Jacquet M., Mallet L.
      Yeast 10:1511-1525(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.

    Entry informationi

    Entry nameiYPC1_YEAST
    AccessioniPrimary (citable) accession number: P38298
    Secondary accession number(s): D6VQH8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3