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P38298 (YPC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alkaline ceramidase YPC1

EC=3.5.1.-
Gene names
Name:YPC1
Ordered Locus Names:YBR183W
ORF Names:YBR1305
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes phytoceramide and also dihydroceramide into phytosphingosine or dihydrosphingosine. Prefers phytoceramide. Has also reverse hydrolysis activity, catalyzing synthesis of phytoceramide and dihydroceramide from palmitic acid and phytosphingosine or dihydrosphingosine. Is not responsible for the breakdown of unsaturated ceramide.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the alkaline ceramidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 316316Alkaline ceramidase YPC1
PRO_0000212466

Regions

Topological domain1 – 6868Cytoplasmic Potential
Transmembrane69 – 8921Helical; Potential
Topological domain90 – 934Lumenal Potential
Transmembrane94 – 11421Helical; Potential
Topological domain115 – 13521Cytoplasmic Potential
Transmembrane136 – 15621Helical; Potential
Topological domain157 – 1604Lumenal Potential
Transmembrane161 – 18121Helical; Potential
Topological domain182 – 19514Cytoplasmic Potential
Transmembrane196 – 21621Helical; Potential
Topological domain217 – 22812Lumenal Potential
Transmembrane229 – 24921Helical; Potential
Topological domain250 – 31667Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P38298 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 90DD942A4522508F

FASTA31636,420
        10         20         30         40         50         60 
MGIFRWNYPE SSVPGVWGET TSTIDWCEEN YVVSPYIAEW SNTLTNSVFI LSAIYTTYSA 

        70         80         90        100        110        120 
YKNKLEKRFL LIGFGYGLVG VGSWLFHMTL KYRFQLLDEL PMIYAMCIPT WSLVCEAKEA 

       130        140        150        160        170        180 
LLNGDNHKKV PLFEQIFIGV IIGLAVTTAS ILYVIYKNVD IHQILFGVQI VVVAATAGSL 

       190        200        210        220        230        240 
TYRYVHDPLA KRNLKASMAL GAILFLSGYI SWLLDIHYCS FWVHVRRSIL ALPLGVLLEP 

       250        260        270        280        290        300 
HGWWHILTGM GIYFYIVSLE HLRVITLNVS CNYQFIWRWK VFPELIWKGR KPSTRYSLEL 

       310 
FGPYVEDQSI EVKKEK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of an alkaline ceramidase from Saccharomyces cerevisiae. An enzyme with reverse (CoA-independent) ceramide synthase activity."
Mao C., Xu R., Bielawska A., Obeid L.M.
J. Biol. Chem. 275:6876-6884(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]"A 12.5 kb fragment of the yeast chromosome II contains two adjacent genes encoding ribosomal proteins and six putative new genes, one of which encodes a putative transcriptional factor."
Demolis N., Jacquet M., Mallet L.
Yeast 10:1511-1525(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF191745 Genomic DNA. Translation: AAF43604.1.
U02073 Genomic DNA. Translation: AAB60277.1.
Z36052 Genomic DNA. Translation: CAA85144.1.
BK006936 Genomic DNA. Translation: DAA07298.1.
PIRS46055.
RefSeqNP_009742.1. NM_001178531.1.

3D structure databases

ProteinModelPortalP38298.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32881. 67 interactions.
DIPDIP-7405N.
IntActP38298. 30 interactions.
MINTMINT-1354145.
STRING4932.YBR183W.

Proteomic databases

PaxDbP38298.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR183W; YBR183W; YBR183W.
GeneID852481.
KEGGsce:YBR183W.

Organism-specific databases

CYGDYBR183w.
SGDS000000387. YPC1.

Phylogenomic databases

eggNOGNOG250726.
GeneTreeENSGT00730000111189.
HOGENOMHOG000192011.
KOK05848.
OMACEAKEAL.
OrthoDBEOG76DV3Q.

Enzyme and pathway databases

BioCycMetaCyc:YBR183W-MONOMER.
YEAST:YBR183W-MONOMER.

Gene expression databases

GenevestigatorP38298.

Family and domain databases

InterProIPR008901. Ceramidase.
[Graphical view]
PfamPF05875. Ceramidase. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio971453.
PROP38298.

Entry information

Entry nameYPC1_YEAST
AccessionPrimary (citable) accession number: P38298
Secondary accession number(s): D6VQH8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 14, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families