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P38298

- YPC1_YEAST

UniProt

P38298 - YPC1_YEAST

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Protein

Alkaline ceramidase YPC1

Gene
YPC1, YBR183W, YBR1305
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes phytoceramide and also dihydroceramide into phytosphingosine or dihydrosphingosine. Prefers phytoceramide. Has also reverse hydrolysis activity, catalyzing synthesis of phytoceramide and dihydroceramide from palmitic acid and phytosphingosine or dihydrosphingosine. Is not responsible for the breakdown of unsaturated ceramide.

GO - Molecular functioni

  1. phytoceramidase activity Source: SGD
  2. sphingosine N-acyltransferase activity Source: SGD

GO - Biological processi

  1. ceramide biosynthetic process Source: SGD
  2. ceramide catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:YBR183W-MONOMER.
YEAST:YBR183W-MONOMER.
ReactomeiREACT_189220. Sphingolipid de novo biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline ceramidase YPC1 (EC:3.5.1.-)
Gene namesi
Name:YPC1
Ordered Locus Names:YBR183W
ORF Names:YBR1305
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR183w.
SGDiS000000387. YPC1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6868Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei69 – 8921Helical; Reviewed predictionAdd
BLAST
Topological domaini90 – 934Lumenal Reviewed prediction
Transmembranei94 – 11421Helical; Reviewed predictionAdd
BLAST
Topological domaini115 – 13521Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei136 – 15621Helical; Reviewed predictionAdd
BLAST
Topological domaini157 – 1604Lumenal Reviewed prediction
Transmembranei161 – 18121Helical; Reviewed predictionAdd
BLAST
Topological domaini182 – 19514Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei196 – 21621Helical; Reviewed predictionAdd
BLAST
Topological domaini217 – 22812Lumenal Reviewed predictionAdd
BLAST
Transmembranei229 – 24921Helical; Reviewed predictionAdd
BLAST
Topological domaini250 – 31667Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. Golgi membrane Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316Alkaline ceramidase YPC1PRO_0000212466Add
BLAST

Proteomic databases

PaxDbiP38298.

Expressioni

Gene expression databases

GenevestigatoriP38298.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
AGP1P253761EBI-2103141,EBI-2357
CHO1P084561EBI-2103141,EBI-14055
ERV29P533371EBI-2103141,EBI-23662
OST1P415431EBI-2103141,EBI-12651
PMP2P409751EBI-2103141,EBI-2043041
RTN1Q049471EBI-2103141,EBI-38020
SAC1P323681EBI-2103141,EBI-16210
SPF1P399861EBI-2103141,EBI-3128
TAF2P232551EBI-2103141,EBI-18862
YHR080CP388001EBI-2103141,EBI-24597

Protein-protein interaction databases

BioGridi32881. 67 interactions.
DIPiDIP-7405N.
IntActiP38298. 30 interactions.
MINTiMINT-1354145.
STRINGi4932.YBR183W.

Structurei

3D structure databases

ProteinModelPortaliP38298.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG250726.
GeneTreeiENSGT00730000111189.
HOGENOMiHOG000192011.
KOiK05848.
OMAiCEAKEAL.
OrthoDBiEOG76DV3Q.

Family and domain databases

InterProiIPR008901. Ceramidase.
[Graphical view]
PfamiPF05875. Ceramidase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38298-1 [UniParc]FASTAAdd to Basket

« Hide

MGIFRWNYPE SSVPGVWGET TSTIDWCEEN YVVSPYIAEW SNTLTNSVFI    50
LSAIYTTYSA YKNKLEKRFL LIGFGYGLVG VGSWLFHMTL KYRFQLLDEL 100
PMIYAMCIPT WSLVCEAKEA LLNGDNHKKV PLFEQIFIGV IIGLAVTTAS 150
ILYVIYKNVD IHQILFGVQI VVVAATAGSL TYRYVHDPLA KRNLKASMAL 200
GAILFLSGYI SWLLDIHYCS FWVHVRRSIL ALPLGVLLEP HGWWHILTGM 250
GIYFYIVSLE HLRVITLNVS CNYQFIWRWK VFPELIWKGR KPSTRYSLEL 300
FGPYVEDQSI EVKKEK 316
Length:316
Mass (Da):36,420
Last modified:October 1, 1994 - v1
Checksum:i90DD942A4522508F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF191745 Genomic DNA. Translation: AAF43604.1.
U02073 Genomic DNA. Translation: AAB60277.1.
Z36052 Genomic DNA. Translation: CAA85144.1.
BK006936 Genomic DNA. Translation: DAA07298.1.
PIRiS46055.
RefSeqiNP_009742.1. NM_001178531.1.

Genome annotation databases

EnsemblFungiiYBR183W; YBR183W; YBR183W.
GeneIDi852481.
KEGGisce:YBR183W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF191745 Genomic DNA. Translation: AAF43604.1 .
U02073 Genomic DNA. Translation: AAB60277.1 .
Z36052 Genomic DNA. Translation: CAA85144.1 .
BK006936 Genomic DNA. Translation: DAA07298.1 .
PIRi S46055.
RefSeqi NP_009742.1. NM_001178531.1.

3D structure databases

ProteinModelPortali P38298.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32881. 67 interactions.
DIPi DIP-7405N.
IntActi P38298. 30 interactions.
MINTi MINT-1354145.
STRINGi 4932.YBR183W.

Proteomic databases

PaxDbi P38298.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR183W ; YBR183W ; YBR183W .
GeneIDi 852481.
KEGGi sce:YBR183W.

Organism-specific databases

CYGDi YBR183w.
SGDi S000000387. YPC1.

Phylogenomic databases

eggNOGi NOG250726.
GeneTreei ENSGT00730000111189.
HOGENOMi HOG000192011.
KOi K05848.
OMAi CEAKEAL.
OrthoDBi EOG76DV3Q.

Enzyme and pathway databases

BioCyci MetaCyc:YBR183W-MONOMER.
YEAST:YBR183W-MONOMER.
Reactomei REACT_189220. Sphingolipid de novo biosynthesis.

Miscellaneous databases

NextBioi 971453.
PROi P38298.

Gene expression databases

Genevestigatori P38298.

Family and domain databases

InterProi IPR008901. Ceramidase.
[Graphical view ]
Pfami PF05875. Ceramidase. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of an alkaline ceramidase from Saccharomyces cerevisiae. An enzyme with reverse (CoA-independent) ceramide synthase activity."
    Mao C., Xu R., Bielawska A., Obeid L.M.
    J. Biol. Chem. 275:6876-6884(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  2. "A 12.5 kb fragment of the yeast chromosome II contains two adjacent genes encoding ribosomal proteins and six putative new genes, one of which encodes a putative transcriptional factor."
    Demolis N., Jacquet M., Mallet L.
    Yeast 10:1511-1525(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiYPC1_YEAST
AccessioniPrimary (citable) accession number: P38298
Secondary accession number(s): D6VQH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 3, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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