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P38295

- MCFS2_YEAST

UniProt

P38295 - MCFS2_YEAST

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Protein
Medium-chain fatty acid ethyl ester synthase/esterase 2
Gene
EHT1, YBR177C, YBR1239
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Displays enzymatic activity both for medium-chain fatty acid (MCFA) ethyl ester synthesis and hydrolysis (esterase activity). MCFA are toxic for yeast and this enzyme could thus be involved in their detoxification by esterification.1 Publication

Catalytic activityi

Acetyl-CoA + an alcohol = CoA + an acetyl ester.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei247 – 2471Charge relay system By similarity
Active sitei395 – 3951Charge relay system By similarity
Active sitei423 – 4231Charge relay system By similarity

GO - Molecular functioni

  1. alcohol O-acetyltransferase activity Source: UniProtKB-EC
  2. alcohol O-butanoyltransferase activity Source: SGD
  3. short-chain carboxylesterase activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. cellular lipid metabolic process Source: SGD
  2. medium-chain fatty acid biosynthetic process Source: SGD
  3. medium-chain fatty acid catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Hydrolase, Serine esterase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YBR177C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Medium-chain fatty acid ethyl ester synthase/esterase 2
Alternative name(s):
Alcohol O-acetyltransferase (EC:2.3.1.84, EC:3.1.1.-)
Ethanol hexanoyl transferase 1
Gene namesi
Name:EHT1
Ordered Locus Names:YBR177C
ORF Names:YBR1239
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR177c.
SGDiS000000381. EHT1.

Subcellular locationi

GO - Cellular componenti

  1. lipid particle Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 451451Medium-chain fatty acid ethyl ester synthase/esterase 2
PRO_0000212454Add
BLAST

Proteomic databases

MaxQBiP38295.
PaxDbiP38295.
PeptideAtlasiP38295.

Expressioni

Gene expression databases

GenevestigatoriP38295.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself1EBI-20890,EBI-20890
ACF2Q121681EBI-20890,EBI-32973
AGP1P253761EBI-20890,EBI-2357
ALO1P547831EBI-20890,EBI-2519
ARO8P530901EBI-20890,EBI-2042933
AYR1P404711EBI-20890,EBI-25213
CYS4P325821EBI-20890,EBI-4167
EMC2P471331EBI-20890,EBI-25568
ERG11P106141EBI-20890,EBI-5127
ERG7P386041EBI-20890,EBI-6572
FAT1P382251EBI-20890,EBI-6819
GDI1P399581EBI-20890,EBI-7517
IPP1P008171EBI-20890,EBI-9338
KES1P358441EBI-20890,EBI-9648
PHO84P252971EBI-20890,EBI-13320
PMP2P409751EBI-20890,EBI-2043041
PMP3P872841EBI-20890,EBI-13555
PPZ1P265701EBI-20890,EBI-13807
RER2P351961EBI-20890,EBI-14943
RSP5P399401EBI-20890,EBI-16219
RTN1Q049471EBI-20890,EBI-38020
SAC1P323681EBI-20890,EBI-16210
SNA3P143591EBI-20890,EBI-26122
SPF1P399861EBI-20890,EBI-3128
TGL1P341631EBI-20890,EBI-19177
TOM20P351801EBI-20890,EBI-12522
UBP1P250371EBI-20890,EBI-19819
VMA8P326101EBI-20890,EBI-20264
YBL039W-BP0C2681EBI-20890,EBI-2044092
YEH1Q078041EBI-20890,EBI-36254
YET1P357231EBI-20890,EBI-26730
YHR003CP387561EBI-20890,EBI-24431
YJU3P283211EBI-20890,EBI-26790

Protein-protein interaction databases

BioGridi32876. 61 interactions.
IntActiP38295. 23 interactions.
MINTiMINT-660335.
STRINGi4932.YBR177C.

Structurei

3D structure databases

ProteinModelPortaliP38295.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0429.
GeneTreeiENSGT00530000062981.
HOGENOMiHOG000248589.
KOiK07019.
OMAiGCCRTKI.
OrthoDBiEOG7X9GHP.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR012020. AB-Hydro_YheT.
IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000952. UPF0017_hydro-like_CS.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005211. Ab_hydro_YheT. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS01133. UPF0017. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38295-1 [UniParc]FASTAAdd to Basket

« Hide

MSEVSKWPAI NPFHWGYNGT VSHIVGENGS IKLHLKDNKE QVDFDEFANK    50
YVPTLKNGAQ FKLSPYLFTG ILQTLYLGAA DFSKKFPVFY GREIVKFSDG 100
GVCTADWLID SWKKDYEFDQ STTSFDKKKF DKDEKATHPE GWPRLQPRTR 150
YLKDNELEEL REVDLPLVVI LHGLAGGSHE PIIRSLAENL SRSGRFQVVV 200
LNTRGCARSK ITTRNLFTAY HTMDIREFLQ REKQRHPDRK LYAVGCSFGA 250
TMLANYLGEE GDKSPLSAAA TLCNPWDLLL SAIRMSQDWW SRTLFSKNIA 300
QFLTRTVQVN MGELGVPNGS LPDHPPTVKN PSFYMFTPEN LIKAKSFKST 350
REFDEVYTAP ALGFPNAMEY YKAASSINRV DTIRVPTLVI NSRDDPVVGP 400
DQPYSIVEKN PRILYCRTDL GGHLAYLDKD NNSWATKAIA EFFTKFDELV 450
V 451
Length:451
Mass (Da):51,255
Last modified:October 1, 1994 - v1
Checksum:i48D48ABF4CC97029
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z36046 Genomic DNA. Translation: CAA85138.1.
BK006936 Genomic DNA. Translation: DAA07292.1.
PIRiS46048.
RefSeqiNP_009736.3. NM_001178525.3.

Genome annotation databases

EnsemblFungiiYBR177C; YBR177C; YBR177C.
GeneIDi852476.
KEGGisce:YBR177C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z36046 Genomic DNA. Translation: CAA85138.1 .
BK006936 Genomic DNA. Translation: DAA07292.1 .
PIRi S46048.
RefSeqi NP_009736.3. NM_001178525.3.

3D structure databases

ProteinModelPortali P38295.
ModBasei Search...

Protein-protein interaction databases

BioGridi 32876. 61 interactions.
IntActi P38295. 23 interactions.
MINTi MINT-660335.
STRINGi 4932.YBR177C.

Proteomic databases

MaxQBi P38295.
PaxDbi P38295.
PeptideAtlasi P38295.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR177C ; YBR177C ; YBR177C .
GeneIDi 852476.
KEGGi sce:YBR177C.

Organism-specific databases

CYGDi YBR177c.
SGDi S000000381. EHT1.

Phylogenomic databases

eggNOGi COG0429.
GeneTreei ENSGT00530000062981.
HOGENOMi HOG000248589.
KOi K07019.
OMAi GCCRTKI.
OrthoDBi EOG7X9GHP.

Enzyme and pathway databases

BioCyci YEAST:YBR177C-MONOMER.

Miscellaneous databases

NextBioi 971438.
PROi P38295.

Gene expression databases

Genevestigatori P38295.

Family and domain databases

Gene3Di 3.40.50.1820. 2 hits.
InterProi IPR012020. AB-Hydro_YheT.
IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000952. UPF0017_hydro-like_CS.
[Graphical view ]
Pfami PF00561. Abhydrolase_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005211. Ab_hydro_YheT. 1 hit.
SUPFAMi SSF53474. SSF53474. 1 hit.
PROSITEi PS01133. UPF0017. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  4. "The Saccharomyces cerevisiae EHT1 and EEB1 genes encode novel enzymes with medium-chain fatty acid ethyl ester synthesis and hydrolysis capacity."
    Saerens S.M.G., Verstrepen K.J., Van Laere S.D., Voet A.R., Van Dijck P., Delvaux F.R., Thevelein J.M.
    J. Biol. Chem. 281:4446-4456(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMCFS2_YEAST
AccessioniPrimary (citable) accession number: P38295
Secondary accession number(s): D6VQH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 9, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2550 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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