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Protein

Medium-chain fatty acid ethyl ester synthase/esterase 2

Gene

EHT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Displays enzymatic activity both for medium-chain fatty acid (MCFA) ethyl ester synthesis and hydrolysis (esterase activity). MCFA are toxic for yeast and this enzyme could thus be involved in their detoxification by esterification.1 Publication

Miscellaneous

Present with 2550 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acetyl-CoA + an alcohol = CoA + an acetyl ester.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei247Charge relay systemBy similarity1
Active sitei395Charge relay systemBy similarity1
Active sitei423Charge relay systemBy similarity1

GO - Molecular functioni

  • acylglycerol lipase activity Source: GO_Central
  • alcohol O-acetyltransferase activity Source: UniProtKB-EC
  • alcohol O-butanoyltransferase activity Source: SGD
  • short-chain carboxylesterase activity Source: SGD

GO - Biological processi

  • cellular lipid metabolic process Source: SGD
  • medium-chain fatty acid biosynthetic process Source: SGD
  • medium-chain fatty acid catabolic process Source: SGD

Keywordsi

Molecular functionAcyltransferase, Hydrolase, Serine esterase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YBR177C-MONOMER.
ReactomeiR-SCE-1483191. Synthesis of PC.

Protein family/group databases

ESTHERiyeast-MCFS2. abh_upf0017.

Names & Taxonomyi

Protein namesi
Recommended name:
Medium-chain fatty acid ethyl ester synthase/esterase 2
Alternative name(s):
Alcohol O-acetyltransferase (EC:2.3.1.84, EC:3.1.1.-)
Ethanol hexanoyl transferase 1
Gene namesi
Name:EHT1
Ordered Locus Names:YBR177C
ORF Names:YBR1239
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR177C.
SGDiS000000381. EHT1.

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002124541 – 451Medium-chain fatty acid ethyl ester synthase/esterase 2Add BLAST451

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki114Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP38295.
PRIDEiP38295.

PTM databases

iPTMnetiP38295.

Interactioni

Protein-protein interaction databases

BioGridi32876. 135 interactors.
IntActiP38295. 23 interactors.
MINTiMINT-660335.
STRINGi4932.YBR177C.

Structurei

3D structure databases

ProteinModelPortaliP38295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini166 – 430AB hydrolase-1Sequence analysisAdd BLAST265

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000062981.
HOGENOMiHOG000248589.
InParanoidiP38295.
KOiK07019.
OMAiGCCRTKI.
OrthoDBiEOG092C2YV9.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiView protein in InterPro
IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000952. AB_hydrolase_4_CS.
IPR012020. ABHD4.
PfamiView protein in Pfam
PF00561. Abhydrolase_1. 1 hit.
PIRSFiPIRSF005211. Ab_hydro_YheT. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiView protein in PROSITE
PS01133. UPF0017. 1 hit.

Sequencei

Sequence statusi: Complete.

P38295-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEVSKWPAI NPFHWGYNGT VSHIVGENGS IKLHLKDNKE QVDFDEFANK
60 70 80 90 100
YVPTLKNGAQ FKLSPYLFTG ILQTLYLGAA DFSKKFPVFY GREIVKFSDG
110 120 130 140 150
GVCTADWLID SWKKDYEFDQ STTSFDKKKF DKDEKATHPE GWPRLQPRTR
160 170 180 190 200
YLKDNELEEL REVDLPLVVI LHGLAGGSHE PIIRSLAENL SRSGRFQVVV
210 220 230 240 250
LNTRGCARSK ITTRNLFTAY HTMDIREFLQ REKQRHPDRK LYAVGCSFGA
260 270 280 290 300
TMLANYLGEE GDKSPLSAAA TLCNPWDLLL SAIRMSQDWW SRTLFSKNIA
310 320 330 340 350
QFLTRTVQVN MGELGVPNGS LPDHPPTVKN PSFYMFTPEN LIKAKSFKST
360 370 380 390 400
REFDEVYTAP ALGFPNAMEY YKAASSINRV DTIRVPTLVI NSRDDPVVGP
410 420 430 440 450
DQPYSIVEKN PRILYCRTDL GGHLAYLDKD NNSWATKAIA EFFTKFDELV

V
Length:451
Mass (Da):51,255
Last modified:October 1, 1994 - v1
Checksum:i48D48ABF4CC97029
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36046 Genomic DNA. Translation: CAA85138.1.
BK006936 Genomic DNA. Translation: DAA07292.1.
PIRiS46048.
RefSeqiNP_009736.3. NM_001178525.3.

Genome annotation databases

EnsemblFungiiYBR177C; YBR177C; YBR177C.
GeneIDi852476.
KEGGisce:YBR177C.

Similar proteinsi

Entry informationi

Entry nameiMCFS2_YEAST
AccessioniPrimary (citable) accession number: P38295
Secondary accession number(s): D6VQH2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 25, 2017
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names