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P38295

- MCFS2_YEAST

UniProt

P38295 - MCFS2_YEAST

Protein

Medium-chain fatty acid ethyl ester synthase/esterase 2

Gene

EHT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Displays enzymatic activity both for medium-chain fatty acid (MCFA) ethyl ester synthesis and hydrolysis (esterase activity). MCFA are toxic for yeast and this enzyme could thus be involved in their detoxification by esterification.1 Publication

    Catalytic activityi

    Acetyl-CoA + an alcohol = CoA + an acetyl ester.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei247 – 2471Charge relay systemBy similarity
    Active sitei395 – 3951Charge relay systemBy similarity
    Active sitei423 – 4231Charge relay systemBy similarity

    GO - Molecular functioni

    1. alcohol O-acetyltransferase activity Source: UniProtKB-EC
    2. alcohol O-butanoyltransferase activity Source: SGD
    3. short-chain carboxylesterase activity Source: SGD

    GO - Biological processi

    1. cellular lipid metabolic process Source: SGD
    2. medium-chain fatty acid biosynthetic process Source: SGD
    3. medium-chain fatty acid catabolic process Source: SGD

    Keywords - Molecular functioni

    Acyltransferase, Hydrolase, Serine esterase, Transferase

    Enzyme and pathway databases

    BioCyciYEAST:YBR177C-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Medium-chain fatty acid ethyl ester synthase/esterase 2
    Alternative name(s):
    Alcohol O-acetyltransferase (EC:2.3.1.84, EC:3.1.1.-)
    Ethanol hexanoyl transferase 1
    Gene namesi
    Name:EHT1
    Ordered Locus Names:YBR177C
    ORF Names:YBR1239
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR177c.
    SGDiS000000381. EHT1.

    Subcellular locationi

    GO - Cellular componenti

    1. lipid particle Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 451451Medium-chain fatty acid ethyl ester synthase/esterase 2PRO_0000212454Add
    BLAST

    Proteomic databases

    MaxQBiP38295.
    PaxDbiP38295.
    PeptideAtlasiP38295.

    Expressioni

    Gene expression databases

    GenevestigatoriP38295.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself1EBI-20890,EBI-20890
    ACF2Q121681EBI-20890,EBI-32973
    AGP1P253761EBI-20890,EBI-2357
    ALO1P547831EBI-20890,EBI-2519
    ARO8P530901EBI-20890,EBI-2042933
    AYR1P404711EBI-20890,EBI-25213
    CYS4P325821EBI-20890,EBI-4167
    EMC2P471331EBI-20890,EBI-25568
    ERG11P106141EBI-20890,EBI-5127
    ERG7P386041EBI-20890,EBI-6572
    FAT1P382251EBI-20890,EBI-6819
    GDI1P399581EBI-20890,EBI-7517
    IPP1P008171EBI-20890,EBI-9338
    KES1P358441EBI-20890,EBI-9648
    PHO84P252971EBI-20890,EBI-13320
    PMP2P409751EBI-20890,EBI-2043041
    PMP3P872841EBI-20890,EBI-13555
    PPZ1P265701EBI-20890,EBI-13807
    RER2P351961EBI-20890,EBI-14943
    RSP5P399401EBI-20890,EBI-16219
    RTN1Q049471EBI-20890,EBI-38020
    SAC1P323681EBI-20890,EBI-16210
    SNA3P143591EBI-20890,EBI-26122
    SPF1P399861EBI-20890,EBI-3128
    TGL1P341631EBI-20890,EBI-19177
    TOM20P351801EBI-20890,EBI-12522
    UBP1P250371EBI-20890,EBI-19819
    VMA8P326101EBI-20890,EBI-20264
    YBL039W-BP0C2681EBI-20890,EBI-2044092
    YEH1Q078041EBI-20890,EBI-36254
    YET1P357231EBI-20890,EBI-26730
    YHR003CP387561EBI-20890,EBI-24431
    YJU3P283211EBI-20890,EBI-26790

    Protein-protein interaction databases

    BioGridi32876. 61 interactions.
    IntActiP38295. 23 interactions.
    MINTiMINT-660335.
    STRINGi4932.YBR177C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38295.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0429.
    GeneTreeiENSGT00530000062981.
    HOGENOMiHOG000248589.
    KOiK07019.
    OMAiGCCRTKI.
    OrthoDBiEOG7X9GHP.

    Family and domain databases

    Gene3Di3.40.50.1820. 2 hits.
    InterProiIPR012020. AB-Hydro_YheT.
    IPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR000952. UPF0017_hydro-like_CS.
    [Graphical view]
    PfamiPF00561. Abhydrolase_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005211. Ab_hydro_YheT. 1 hit.
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS01133. UPF0017. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38295-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEVSKWPAI NPFHWGYNGT VSHIVGENGS IKLHLKDNKE QVDFDEFANK    50
    YVPTLKNGAQ FKLSPYLFTG ILQTLYLGAA DFSKKFPVFY GREIVKFSDG 100
    GVCTADWLID SWKKDYEFDQ STTSFDKKKF DKDEKATHPE GWPRLQPRTR 150
    YLKDNELEEL REVDLPLVVI LHGLAGGSHE PIIRSLAENL SRSGRFQVVV 200
    LNTRGCARSK ITTRNLFTAY HTMDIREFLQ REKQRHPDRK LYAVGCSFGA 250
    TMLANYLGEE GDKSPLSAAA TLCNPWDLLL SAIRMSQDWW SRTLFSKNIA 300
    QFLTRTVQVN MGELGVPNGS LPDHPPTVKN PSFYMFTPEN LIKAKSFKST 350
    REFDEVYTAP ALGFPNAMEY YKAASSINRV DTIRVPTLVI NSRDDPVVGP 400
    DQPYSIVEKN PRILYCRTDL GGHLAYLDKD NNSWATKAIA EFFTKFDELV 450
    V 451
    Length:451
    Mass (Da):51,255
    Last modified:October 1, 1994 - v1
    Checksum:i48D48ABF4CC97029
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z36046 Genomic DNA. Translation: CAA85138.1.
    BK006936 Genomic DNA. Translation: DAA07292.1.
    PIRiS46048.
    RefSeqiNP_009736.3. NM_001178525.3.

    Genome annotation databases

    EnsemblFungiiYBR177C; YBR177C; YBR177C.
    GeneIDi852476.
    KEGGisce:YBR177C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z36046 Genomic DNA. Translation: CAA85138.1 .
    BK006936 Genomic DNA. Translation: DAA07292.1 .
    PIRi S46048.
    RefSeqi NP_009736.3. NM_001178525.3.

    3D structure databases

    ProteinModelPortali P38295.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32876. 61 interactions.
    IntActi P38295. 23 interactions.
    MINTi MINT-660335.
    STRINGi 4932.YBR177C.

    Proteomic databases

    MaxQBi P38295.
    PaxDbi P38295.
    PeptideAtlasi P38295.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR177C ; YBR177C ; YBR177C .
    GeneIDi 852476.
    KEGGi sce:YBR177C.

    Organism-specific databases

    CYGDi YBR177c.
    SGDi S000000381. EHT1.

    Phylogenomic databases

    eggNOGi COG0429.
    GeneTreei ENSGT00530000062981.
    HOGENOMi HOG000248589.
    KOi K07019.
    OMAi GCCRTKI.
    OrthoDBi EOG7X9GHP.

    Enzyme and pathway databases

    BioCyci YEAST:YBR177C-MONOMER.

    Miscellaneous databases

    NextBioi 971438.
    PROi P38295.

    Gene expression databases

    Genevestigatori P38295.

    Family and domain databases

    Gene3Di 3.40.50.1820. 2 hits.
    InterProi IPR012020. AB-Hydro_YheT.
    IPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR000952. UPF0017_hydro-like_CS.
    [Graphical view ]
    Pfami PF00561. Abhydrolase_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005211. Ab_hydro_YheT. 1 hit.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS01133. UPF0017. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    4. "The Saccharomyces cerevisiae EHT1 and EEB1 genes encode novel enzymes with medium-chain fatty acid ethyl ester synthesis and hydrolysis capacity."
      Saerens S.M.G., Verstrepen K.J., Van Laere S.D., Voet A.R., Van Dijck P., Delvaux F.R., Thevelein J.M.
      J. Biol. Chem. 281:4446-4456(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMCFS2_YEAST
    AccessioniPrimary (citable) accession number: P38295
    Secondary accession number(s): D6VQH2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2550 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3