P38289 (EXO5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exonuclease V, mitochondrial
Short name=Exo V
EC=3.1.11.-

Alternative name(s):
Defects in morphology protein 1

Gene names

Name:	EXO5
Synonyms:	DEM1
Ordered Locus Names:	YBR163W
ORF Names:	YBR1215

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Single strand DNA specific 5' exonuclease involved in mitochondrial DNA replication and recombination. Releases dinucleotides as main products of catalysis. Has the capacity to slide across 5'double-stranded DNA or 5'RNA sequences and resumes cutting two nucleotides downstream of the double-stranded-to-single-stranded junction or RNA-to-DNA junction, respectively. Ref.3 Ref.4 Ref.7
Cofactor	Magnesium. Ref.4
Subunit structure	Monomer. Ref.7
Subcellular location	Mitochondrion Ref.5.
Miscellaneous	Present with 1420 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the EXO5 family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.8. Ref.4

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	DNA-binding Magnesium
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial genome maintenance Inferred from mutant phenotype Ref.7. Source: SGD
Cellular component	mitochondrion Inferred from direct assay Ref.5. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW single-stranded DNA specific 5'-3' exodeoxyribonuclease activity Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LSB3	P43603	1	EBI-20869,EBI-22980
RVS167	P39743	1	EBI-20869,EBI-14500
YSC84	P32793	1	EBI-20869,EBI-24460

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 26	26	Mitochondrion Potential
	Chain	27 – 585	559	Exonuclease V, mitochondrial		PRO_0000202501
Experimental info
	Mutagenesis	270	1	D → A: Impairs exonuclease activity. Ref.7
	Mutagenesis	320	1	D → A: Impairs exonuclease activity. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P38289 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 92DBE35B819E0AFB	FASTA	585	67,570
	10 20 30 40 50 60 MLGRALINKY GFLIHPRRFV HLNDKSLDGT FILPSKKNHM YDVPTNDPSG ILNASDIDRI 70 80 90 100 110 120 NNLPFFDNTS PTKETNTKEG ALLSEKLASV KELFGEDPEN PSFINYRFPR GLENPYFDIQ 130 140 150 160 170 180 VNQLKKKRLS VTQLCTTQNW CELRNFYDFY SQNLSNQLLN LKFQVQKGKK IHKSLEDETH 190 200 210 220 230 240 PELNQYKSFT HNFLALTKLS MDIDNDMDAL LDNWFNSINR LVSLFTKGDG HAREIVCHGF 250 260 270 280 290 300 INLEDGKLVE HLLNSDSKTK ENVIISGVID HLTLRNRHNH QVQKGAAHLD TEYQSWGNIL 310 320 330 340 350 360 TNLLSNLKEL KSNNEIVISD IKTRSVPKIP SIESVIESSK LQTMYYKFFF SHLSQDMTQT 370 380 390 400 410 420 YHSFLINAQR RGLDVDAPIN PTKILTFILT NPLFANDVKN LLYGLPINHS AFDNDAKGSN 430 440 450 460 470 480 TFDMTAFNDL LDRGPTSFNV PIEQDEDSSE STKCVSLRDY GHFYTKWKTP LTLKYFAARL 490 500 510 520 530 540 SQIYFIVGNL VSNDLMIEYY YHNDNFHNII FPYDPLKLGT HAHDSAMVWF GGRDMHPIEP 550 560 570 580 TQKNFNTYCK FCDYRHVCSW KNKNELKLID LGKELKKIIL ESSMK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach." Entian K.-D., Schuster T., Hegemann J.H., Becher D., Feldmann H., Gueldener U., Goetz R., Hansen M., Hollenberg C.P., Jansen G., Kramer W., Klein S., Koetter P., Kricke J., Launhardt H., Mannhaupt G., Maierl A., Meyer P. , Mewes W., Munder T., Niedenthal R.K., Ramezani Rad M., Roehmer A., Roemer A., Rose M., Schaefer B., Siegler M.-L., Vetter J., Wilhelm N., Wolf K., Zimmermann F.K., Zollner A., Hinnen A. Mol. Gen. Genet. 262:683-702(1999) [PubMed: 10628851] [Abstract] Cited for: FUNCTION.
[4]	"Exonuclease V from Saccharomyces cerevisiae. A 5'-3'-deoxyribonuclease that produces dinucleotides in a sequential fashion." Burgers P.M., Bauer G.A., Tam L. J. Biol. Chem. 263:8099-8105(1988) [PubMed: 3286646] [Abstract] Cited for: FUNCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Yeast exonuclease 5 is essential for mitochondrial genome maintenance." Burgers P.M., Stith C.M., Yoder B.L., Sparks J.L. Mol. Cell. Biol. 30:1457-1466(2010) [PubMed: 20086101] [Abstract] Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF ASP-270 AND ASP-320.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z36032 Genomic DNA. Translation: CAA85123.1. BK006936 Genomic DNA. Translation: DAA07279.1.
PIR	S46034.
RefSeq	NP_009722.1. NM_001178511.1.
3D structure databases
ProteinModelPortal	P38289.
ModBase	Search...
Protein-protein interaction databases
IntAct	P38289. 3 interactions.
MINT	MINT-1552731.
STRING	P38289.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR163W; YBR163W; YBR163W.
GeneID	852461.
KEGG	sce:YBR163W.
NMPDR	fig|4932.3.peg.428.
Organism-specific databases
CYGD	YBR163w.
SGD	S000000367. EXO5.
Phylogenomic databases
eggNOG	fuNOG05643.
HOGENOM	HBG204182.
OMA	TDVKTRS.
OrthoDB	EOG4M0J9Q.
Gene expression databases
ArrayExpress	P38289.
Genevestigator	P38289.
GermOnline	YBR163W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016610. Defects_morphology_1. IPR019190. Defects_morphology_1-like_mit. [Graphical view]
Pfam	PF09810. Morph_protein1. 2 hits. [Graphical view]
PIRSF	PIRSF013220. UCP013220. 1 hit.
ProtoNet	Search...
Other
NextBio	971399.

Entry information

Entry name

EXO5_YEAST

Accession

Primary (citable) accession number: P38289
Secondary accession number(s): D6VQF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 14, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program