Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mannosyl phosphorylinositol ceramide synthase CSH1

Gene

CSH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the synthesis of mannosyl phosphorylinositol ceramide. Catalyzes the addition of mannosyl to phosphorylinositol ceramide.1 Publication

GO - Molecular functioni

  • mannosyltransferase activity Source: SGD
  • transferase activity, transferring glycosyl groups Source: SGD

GO - Biological processi

  • glycosphingolipid biosynthetic process Source: SGD
  • mannosylation Source: GOC
  • sphingolipid biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17702.
YEAST:G3O-29111-MONOMER.

Protein family/group databases

CAZyiGT32. Glycosyltransferase Family 32.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl phosphorylinositol ceramide synthase CSH1 (EC:2.-.-.-)
Alternative name(s):
CSG1/SUR1 homolog 1
Gene namesi
Name:CSH1
Ordered Locus Names:YBR161W
ORF Names:YBR1212
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR161W.
SGDiS000000365. CSH1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2721HelicalSequence analysisAdd
BLAST
Transmembranei274 – 29421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Mannosyl phosphorylinositol ceramide synthase CSH1PRO_0000079395Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei354 – 3541PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PeptideAtlasiP38287.

PTM databases

iPTMnetiP38287.

Interactioni

Subunit structurei

Heterodimer of CSH1 and CSG2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CSG2P352062EBI-20861,EBI-2051140

Protein-protein interaction databases

BioGridi32860. 40 interactions.
DIPiDIP-5144N.
IntActiP38287. 3 interactions.
MINTiMINT-511838.

Structurei

3D structure databases

ProteinModelPortaliP38287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 32 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000007232.
HOGENOMiHOG000200927.
InParanoidiP38287.
OMAiEASHESW.
OrthoDBiEOG7D8665.

Family and domain databases

InterProiIPR007577. GlycoTrfase_DXD_sugar-bd_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF04488. Gly_transf_sug. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

P38287-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKELKILII ANIALLISII HYTFDLLTLC IDDTSKDALT DEQLNPPNGF
60 70 80 90 100
NSTFYESPPQ LIPKIIHQTY KTNDIPEQWV KGRQKCIDLH PDYTYILWTD
110 120 130 140 150
EMSDTFIKQE YPWFLDTFRS YEYPIERADA IRYFILSHYG GIYIDLDDGC
160 170 180 190 200
ERRLDPLLKV PAFLRKTSPT GVSNDVMGSV PRHPFFLKVI KSLKHYKKNW
210 220 230 240 250
YIPYMTIMGS TGPLFISVVW KQYKRWSNTA ENGAVRILQP ADYKMHNNSF
260 270 280 290 300
FSISKGSSWH TGDANFMKTL ENHILSCVVT GFIFGFFILY GEFTFYTWLC
310 320 330 340 350
SGPFNNKRYY IQWLSDKFKL HKWKLTSSYK NKEKRRNPTR HEYNSRGKRL
360 370
RKDSNIPYDS VFLDIEKNHA KFTDLT
Length:376
Mass (Da):44,382
Last modified:October 1, 1994 - v1
Checksum:i34D992039DA0B796
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80224 Genomic DNA. Translation: CAA56510.1.
Z36030 Genomic DNA. Translation: CAA85120.1.
AY692764 Genomic DNA. Translation: AAT92783.1.
BK006936 Genomic DNA. Translation: DAA07276.1.
PIRiS46032.
RefSeqiNP_009719.3. NM_001178509.3.

Genome annotation databases

EnsemblFungiiYBR161W; YBR161W; YBR161W.
GeneIDi852458.
KEGGisce:YBR161W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80224 Genomic DNA. Translation: CAA56510.1.
Z36030 Genomic DNA. Translation: CAA85120.1.
AY692764 Genomic DNA. Translation: AAT92783.1.
BK006936 Genomic DNA. Translation: DAA07276.1.
PIRiS46032.
RefSeqiNP_009719.3. NM_001178509.3.

3D structure databases

ProteinModelPortaliP38287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32860. 40 interactions.
DIPiDIP-5144N.
IntActiP38287. 3 interactions.
MINTiMINT-511838.

Protein family/group databases

CAZyiGT32. Glycosyltransferase Family 32.

PTM databases

iPTMnetiP38287.

Proteomic databases

PeptideAtlasiP38287.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR161W; YBR161W; YBR161W.
GeneIDi852458.
KEGGisce:YBR161W.

Organism-specific databases

EuPathDBiFungiDB:YBR161W.
SGDiS000000365. CSH1.

Phylogenomic databases

GeneTreeiENSGT00390000007232.
HOGENOMiHOG000200927.
InParanoidiP38287.
OMAiEASHESW.
OrthoDBiEOG7D8665.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17702.
YEAST:G3O-29111-MONOMER.

Miscellaneous databases

NextBioi971390.
PROiP38287.

Family and domain databases

InterProiIPR007577. GlycoTrfase_DXD_sugar-bd_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF04488. Gly_transf_sug. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 5.6 kb fragment of chromosome II from Saccharomyces cerevisiae reveals two new open reading frames next to CDC28."
    Baur S., Becker J., Li Z., Niegemann E., Wehner E., Wolter R., Brendel M.
    Yeast 11:455-458(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Csg1p and newly identified Csh1p function in mannosylinositol phosphorylceramide synthesis by interacting with Csg2p."
    Uemura S., Kihara A., Inokuchi J., Igarashi Y.
    J. Biol. Chem. 278:45049-45055(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.

Entry informationi

Entry nameiCSH1_YEAST
AccessioniPrimary (citable) accession number: P38287
Secondary accession number(s): D6VQF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 11, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 195 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.