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P38285

- AMN1_YEAST

UniProt

P38285 - AMN1_YEAST

Protein

Antagonist of mitotic exit network protein 1

Gene

AMN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Negative regulator of the mitotic exit network (MEN), required for multiple cell cycle checkpoints. Acts in the daughter cell to inhibit the mitotic exit pathway once MEN has executed its function. Through its binding ability to TEM1, interferes with the TEM1-CDC5 association, required for CDC5 kinase activation and MEN activation. Required for daughter cell separation and chromosome stability. Involved in copper sensitivity.5 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. mitotic cell cycle checkpoint Source: SGD
    2. mitotic nuclear division Source: UniProtKB-KW
    3. negative regulation of exit from mitosis Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29108-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Antagonist of mitotic exit network protein 1
    Alternative name(s):
    Chromosome stability protein 13
    Increased copper-sensitivity protein 4
    Gene namesi
    Name:AMN1
    Synonyms:CST13, ICS4
    Ordered Locus Names:YBR158W
    ORF Names:YBR1208
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR158w.
    SGDiS000000362. AMN1.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud Source: SGD
    2. cytoplasm Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 549549Antagonist of mitotic exit network protein 1PRO_0000202500Add
    BLAST

    Proteomic databases

    MaxQBiP38285.
    PaxDbiP38285.
    PeptideAtlasiP38285.

    Expressioni

    Inductioni

    Expressed in daughter cells after execution of mitotic exit. Expression is controlled by the ACE2 and SWI5 transcription factors.2 Publications

    Gene expression databases

    GenevestigatoriP38285.

    Interactioni

    Subunit structurei

    Interacts with TEM1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TEM1P389876EBI-20853,EBI-19113

    Protein-protein interaction databases

    BioGridi32857. 41 interactions.
    DIPiDIP-4930N.
    IntActiP38285. 7 interactions.
    MINTiMINT-550873.
    STRINGi4932.YBR158W.

    Structurei

    3D structure databases

    ProteinModelPortaliP38285.
    SMRiP38285. Positions 330-371, 442-487.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AMN1 family.Curated

    Phylogenomic databases

    eggNOGiNOG275193.
    HOGENOMiHOG000033921.
    KOiK15081.
    OMAiAGCHITD.
    OrthoDBiEOG78H42W.

    Sequencei

    Sequence statusi: Complete.

    P38285-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLERVSSNG SFKRGRDIQS LESPCTRPLK KMSPSPSFTS LKMEKPFKDI    50
    VRKYGGHLHQ SSYNPGSSKV ELVRPDLSLK TDQSFLQSSV QTTPNKKSCN 100
    EYLSTPEATP LKNTATENAW ATSRVVSASS LSIVTPTEIK NILVDEFSEL 150
    KLGQPLTAQH QRSHAVFEIP EIVENIIKMI VSLESANIPK ERPCLRRNPQ 200
    SYEHSLLMYK DEERAKKAWS AAQQLRDPPL VGHKEKKQGA LFSCMMVNRL 250
    WLNVTRPFLF KSLHFKSVHN FKEFLRTSQE TTQVMRPSHF ILHKLHQVTQ 300
    PDIERLSRME CQNLKWLEFY VCPRITPPLS WFDNLHKLEK LIIPGNKNID 350
    DNFLLRLSQS IPNLKHLVLR ACDNVSDSGV VCIALNCPKL KTFNIGRHRR 400
    GNLITSVSLV ALGKYTQVET VGFAGCDVDD AGIWEFARLN GKNVERLSLN 450
    SCRLLTDYSL PILFALNSFP NLAVLEIRNL DKITDVRHFV KYNLWKKSLD 500
    APILIEACER ITKLIDQEEN RVKRINSLVA LKDMTAWVNA DDEIENNVD 549
    Length:549
    Mass (Da):62,686
    Last modified:October 1, 1994 - v1
    Checksum:iEA1EC675D6A84F20
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z36027 Genomic DNA. Translation: CAA85117.1.
    AY723760 Genomic DNA. Translation: AAU09677.1.
    BK006936 Genomic DNA. Translation: DAA07273.1.
    PIRiS46029.
    RefSeqiNP_009716.1. NM_001178506.1.

    Genome annotation databases

    EnsemblFungiiYBR158W; YBR158W; YBR158W.
    GeneIDi852455.
    KEGGisce:YBR158W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z36027 Genomic DNA. Translation: CAA85117.1 .
    AY723760 Genomic DNA. Translation: AAU09677.1 .
    BK006936 Genomic DNA. Translation: DAA07273.1 .
    PIRi S46029.
    RefSeqi NP_009716.1. NM_001178506.1.

    3D structure databases

    ProteinModelPortali P38285.
    SMRi P38285. Positions 330-371, 442-487.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32857. 41 interactions.
    DIPi DIP-4930N.
    IntActi P38285. 7 interactions.
    MINTi MINT-550873.
    STRINGi 4932.YBR158W.

    Proteomic databases

    MaxQBi P38285.
    PaxDbi P38285.
    PeptideAtlasi P38285.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR158W ; YBR158W ; YBR158W .
    GeneIDi 852455.
    KEGGi sce:YBR158W.

    Organism-specific databases

    CYGDi YBR158w.
    SGDi S000000362. AMN1.

    Phylogenomic databases

    eggNOGi NOG275193.
    HOGENOMi HOG000033921.
    KOi K15081.
    OMAi AGCHITD.
    OrthoDBi EOG78H42W.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29108-MONOMER.

    Miscellaneous databases

    NextBioi 971381.

    Gene expression databases

    Genevestigatori P38285.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: FUNCTION.
    5. "New yeast genes important for chromosome integrity and segregation identified by dosage effects on genome stability."
      Ouspenski I.I., Elledge S.J., Brinkley B.R.
      Nucleic Acids Res. 27:3001-3008(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p."
      Doolin M.-T., Johnson A.L., Johnston L.H., Butler G.
      Mol. Microbiol. 40:422-432(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    7. "Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling."
      Wang Y., Shirogane T., Liu D., Harper J.W., Elledge S.J.
      Cell 112:697-709(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION, INTERACTION WITH TEM1.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Trans-acting regulatory variation in Saccharomyces cerevisiae and the role of transcription factors."
      Yvert G., Brem R.B., Whittle J., Akey J.M., Foss E., Smith E.N., Mackelprang R., Kruglyak L.
      Nat. Genet. 35:57-64(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Phosphatase 2A negatively regulates mitotic exit in Saccharomyces cerevisiae."
      Wang Y., Ng T.-Y.
      Mol. Biol. Cell 17:80-89(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiAMN1_YEAST
    AccessioniPrimary (citable) accession number: P38285
    Secondary accession number(s): D6VQF3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2020 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3