P38285 (AMN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Antagonist of mitotic exit network protein 1

Alternative name(s):
Chromosome stability protein 13
Increased copper-sensitivity protein 4

Gene names

Name:	AMN1
Synonyms:	CST13, ICS4
Ordered Locus Names:	YBR158W
ORF Names:	YBR1208

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negative regulator of the mitotic exit network (MEN), required for multiple cell cycle checkpoints. Acts in the daughter cell to inhibit the mitotic exit pathway once MEN has executed its function. Through its binding ability to TEM1, interferes with the TEM1-CDC5 association, required for CDC5 kinase activation and MEN activation. Required for daughter cell separation and chromosome stability. Involved in copper sensitivity. Ref.4 Ref.5 Ref.7 Ref.10 Ref.11
Subunit structure	Interacts with TEM1. Ref.7
Subcellular location	Cytoplasm. Nucleus Ref.7 Ref.8.
Induction	Expressed in daughter cells after execution of mitotic exit. Expression is controlled by the ACE2 and SWI5 transcription factors. Ref.6 Ref.7
Miscellaneous	Present with 2020 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the AMN1 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW mitotic cell cycle checkpoint Inferred from mutant phenotype Ref.7. Source: SGD negative regulation of exit from mitosis Inferred from mutant phenotype Ref.7. Source: SGD
Cellular_component	cellular bud Inferred from direct assay Ref.7. Source: SGD cytoplasm Inferred from direct assay Ref.7. Source: SGD nucleus Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TEM1	P38987	6	EBI-20853,EBI-19113

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Antagonist of mitotic exit network protein 1		PRO_0000202500

Sequences

	Sequence		Length	Mass (Da)
	P38285 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: EA1EC675D6A84F20	FASTA	549	62,686
	10 20 30 40 50 60 MKLERVSSNG SFKRGRDIQS LESPCTRPLK KMSPSPSFTS LKMEKPFKDI VRKYGGHLHQ 70 80 90 100 110 120 SSYNPGSSKV ELVRPDLSLK TDQSFLQSSV QTTPNKKSCN EYLSTPEATP LKNTATENAW 130 140 150 160 170 180 ATSRVVSASS LSIVTPTEIK NILVDEFSEL KLGQPLTAQH QRSHAVFEIP EIVENIIKMI 190 200 210 220 230 240 VSLESANIPK ERPCLRRNPQ SYEHSLLMYK DEERAKKAWS AAQQLRDPPL VGHKEKKQGA 250 260 270 280 290 300 LFSCMMVNRL WLNVTRPFLF KSLHFKSVHN FKEFLRTSQE TTQVMRPSHF ILHKLHQVTQ 310 320 330 340 350 360 PDIERLSRME CQNLKWLEFY VCPRITPPLS WFDNLHKLEK LIIPGNKNID DNFLLRLSQS 370 380 390 400 410 420 IPNLKHLVLR ACDNVSDSGV VCIALNCPKL KTFNIGRHRR GNLITSVSLV ALGKYTQVET 430 440 450 460 470 480 VGFAGCDVDD AGIWEFARLN GKNVERLSLN SCRLLTDYSL PILFALNSFP NLAVLEIRNL 490 500 510 520 530 540 DKITDVRHFV KYNLWKKSLD APILIEACER ITKLIDQEEN RVKRINSLVA LKDMTAWVNA DDEIENNVD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach." Entian K.-D., Schuster T., Hegemann J.H., Becher D., Feldmann H., Gueldener U., Goetz R., Hansen M., Hollenberg C.P., Jansen G., Kramer W., Klein S., Koetter P., Kricke J., Launhardt H., Mannhaupt G., Maierl A., Meyer P. , Mewes W., Munder T., Niedenthal R.K., Ramezani Rad M., Roehmer A., Roemer A., Rose M., Schaefer B., Siegler M.-L., Vetter J., Wilhelm N., Wolf K., Zimmermann F.K., Zollner A., Hinnen A. Mol. Gen. Genet. 262:683-702(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"New yeast genes important for chromosome integrity and segregation identified by dosage effects on genome stability." Ouspenski I.I., Elledge S.J., Brinkley B.R. Nucleic Acids Res. 27:3001-3008(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p." Doolin M.-T., Johnson A.L., Johnston L.H., Butler G. Mol. Microbiol. 40:422-432(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[7]	"Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling." Wang Y., Shirogane T., Liu D., Harper J.W., Elledge S.J. Cell 112:697-709(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION, INTERACTION WITH TEM1.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Trans-acting regulatory variation in Saccharomyces cerevisiae and the role of transcription factors." Yvert G., Brem R.B., Whittle J., Akey J.M., Foss E., Smith E.N., Mackelprang R., Kruglyak L. Nat. Genet. 35:57-64(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"Phosphatase 2A negatively regulates mitotic exit in Saccharomyces cerevisiae." Wang Y., Ng T.-Y. Mol. Biol. Cell 17:80-89(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z36027 Genomic DNA. Translation: CAA85117.1. AY723760 Genomic DNA. Translation: AAU09677.1. BK006936 Genomic DNA. Translation: DAA07273.1.
PIR	S46029.
RefSeq	NP_009716.1. NM_001178506.1.
3D structure databases
ProteinModelPortal	P38285.
SMR	P38285. Positions 309-429.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4930N.
IntAct	P38285. 7 interactions.
MINT	MINT-550873.
STRING	4932.YBR158W.
Proteomic databases
PaxDb	P38285.
PeptideAtlas	P38285.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR158W; YBR158W; YBR158W.
GeneID	852455.
KEGG	sce:YBR158W.
Organism-specific databases
CYGD	YBR158w.
SGD	S000000362. AMN1.
Phylogenomic databases
eggNOG	NOG275193.
HOGENOM	HOG000033921.
KO	K15081.
OMA	AGCHITD.
OrthoDB	EOG40VZZJ.
Gene expression databases
Genevestigator	P38285.
GermOnline	YBR158W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	971381.

Entry information

Entry name

AMN1_YEAST

Accession

Primary (citable) accession number: P38285
Secondary accession number(s): D6VQF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program