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P38285 (AMN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Antagonist of mitotic exit network protein 1
Alternative name(s):
Chromosome stability protein 13
Increased copper-sensitivity protein 4
Gene names
Name:AMN1
Synonyms:CST13, ICS4
Ordered Locus Names:YBR158W
ORF Names:YBR1208
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length549 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Negative regulator of the mitotic exit network (MEN), required for multiple cell cycle checkpoints. Acts in the daughter cell to inhibit the mitotic exit pathway once MEN has executed its function. Through its binding ability to TEM1, interferes with the TEM1-CDC5 association, required for CDC5 kinase activation and MEN activation. Required for daughter cell separation and chromosome stability. Involved in copper sensitivity. Ref.4 Ref.5 Ref.7 Ref.10 Ref.11

Subunit structure

Interacts with TEM1. Ref.7

Subcellular location

Cytoplasm. Nucleus Ref.7 Ref.8.

Induction

Expressed in daughter cells after execution of mitotic exit. Expression is controlled by the ACE2 and SWI5 transcription factors. Ref.6 Ref.7

Miscellaneous

Present with 2020 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the AMN1 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TEM1P389876EBI-20853,EBI-19113

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 549549Antagonist of mitotic exit network protein 1
PRO_0000202500

Sequences

Sequence LengthMass (Da)Tools
P38285 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: EA1EC675D6A84F20

FASTA54962,686
        10         20         30         40         50         60 
MKLERVSSNG SFKRGRDIQS LESPCTRPLK KMSPSPSFTS LKMEKPFKDI VRKYGGHLHQ 

        70         80         90        100        110        120 
SSYNPGSSKV ELVRPDLSLK TDQSFLQSSV QTTPNKKSCN EYLSTPEATP LKNTATENAW 

       130        140        150        160        170        180 
ATSRVVSASS LSIVTPTEIK NILVDEFSEL KLGQPLTAQH QRSHAVFEIP EIVENIIKMI 

       190        200        210        220        230        240 
VSLESANIPK ERPCLRRNPQ SYEHSLLMYK DEERAKKAWS AAQQLRDPPL VGHKEKKQGA 

       250        260        270        280        290        300 
LFSCMMVNRL WLNVTRPFLF KSLHFKSVHN FKEFLRTSQE TTQVMRPSHF ILHKLHQVTQ 

       310        320        330        340        350        360 
PDIERLSRME CQNLKWLEFY VCPRITPPLS WFDNLHKLEK LIIPGNKNID DNFLLRLSQS 

       370        380        390        400        410        420 
IPNLKHLVLR ACDNVSDSGV VCIALNCPKL KTFNIGRHRR GNLITSVSLV ALGKYTQVET 

       430        440        450        460        470        480 
VGFAGCDVDD AGIWEFARLN GKNVERLSLN SCRLLTDYSL PILFALNSFP NLAVLEIRNL 

       490        500        510        520        530        540 
DKITDVRHFV KYNLWKKSLD APILIEACER ITKLIDQEEN RVKRINSLVA LKDMTAWVNA 


DDEIENNVD 

« Hide

References

« Hide 'large scale' references
[1]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach."
Entian K.-D., Schuster T., Hegemann J.H., Becher D., Feldmann H., Gueldener U., Goetz R., Hansen M., Hollenberg C.P., Jansen G., Kramer W., Klein S., Koetter P., Kricke J., Launhardt H., Mannhaupt G., Maierl A., Meyer P. expand/collapse author list , Mewes W., Munder T., Niedenthal R.K., Ramezani Rad M., Roehmer A., Roemer A., Rose M., Schaefer B., Siegler M.-L., Vetter J., Wilhelm N., Wolf K., Zimmermann F.K., Zollner A., Hinnen A.
Mol. Gen. Genet. 262:683-702(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"New yeast genes important for chromosome integrity and segregation identified by dosage effects on genome stability."
Ouspenski I.I., Elledge S.J., Brinkley B.R.
Nucleic Acids Res. 27:3001-3008(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p."
Doolin M.-T., Johnson A.L., Johnston L.H., Butler G.
Mol. Microbiol. 40:422-432(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[7]"Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling."
Wang Y., Shirogane T., Liu D., Harper J.W., Elledge S.J.
Cell 112:697-709(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION, INTERACTION WITH TEM1.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Trans-acting regulatory variation in Saccharomyces cerevisiae and the role of transcription factors."
Yvert G., Brem R.B., Whittle J., Akey J.M., Foss E., Smith E.N., Mackelprang R., Kruglyak L.
Nat. Genet. 35:57-64(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Phosphatase 2A negatively regulates mitotic exit in Saccharomyces cerevisiae."
Wang Y., Ng T.-Y.
Mol. Biol. Cell 17:80-89(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z36027 Genomic DNA. Translation: CAA85117.1.
AY723760 Genomic DNA. Translation: AAU09677.1.
BK006936 Genomic DNA. Translation: DAA07273.1.
PIRS46029.
RefSeqNP_009716.1. NM_001178506.1.

3D structure databases

ProteinModelPortalP38285.
SMRP38285. Positions 330-371, 442-487.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32857. 41 interactions.
DIPDIP-4930N.
IntActP38285. 7 interactions.
MINTMINT-550873.
STRING4932.YBR158W.

Proteomic databases

MaxQBP38285.
PaxDbP38285.
PeptideAtlasP38285.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR158W; YBR158W; YBR158W.
GeneID852455.
KEGGsce:YBR158W.

Organism-specific databases

CYGDYBR158w.
SGDS000000362. AMN1.

Phylogenomic databases

eggNOGNOG275193.
HOGENOMHOG000033921.
KOK15081.
OMAAGCHITD.
OrthoDBEOG78H42W.

Enzyme and pathway databases

BioCycYEAST:G3O-29108-MONOMER.

Gene expression databases

GenevestigatorP38285.

Family and domain databases

ProtoNetSearch...

Other

NextBio971381.

Entry information

Entry nameAMN1_YEAST
AccessionPrimary (citable) accession number: P38285
Secondary accession number(s): D6VQF3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 11, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families