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P38285

- AMN1_YEAST

UniProt

P38285 - AMN1_YEAST

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Protein

Antagonist of mitotic exit network protein 1

Gene

AMN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Negative regulator of the mitotic exit network (MEN), required for multiple cell cycle checkpoints. Acts in the daughter cell to inhibit the mitotic exit pathway once MEN has executed its function. Through its binding ability to TEM1, interferes with the TEM1-CDC5 association, required for CDC5 kinase activation and MEN activation. Required for daughter cell separation and chromosome stability. Involved in copper sensitivity.5 Publications

GO - Biological processi

  1. mitotic cell cycle checkpoint Source: SGD
  2. mitotic nuclear division Source: UniProtKB-KW
  3. negative regulation of exit from mitosis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-29108-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Antagonist of mitotic exit network protein 1
Alternative name(s):
Chromosome stability protein 13
Increased copper-sensitivity protein 4
Gene namesi
Name:AMN1
Synonyms:CST13, ICS4
Ordered Locus Names:YBR158W
ORF Names:YBR1208
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR158w.
SGDiS000000362. AMN1.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud Source: SGD
  2. cytoplasm Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 549549Antagonist of mitotic exit network protein 1PRO_0000202500Add
BLAST

Proteomic databases

MaxQBiP38285.
PaxDbiP38285.
PeptideAtlasiP38285.

Expressioni

Inductioni

Expressed in daughter cells after execution of mitotic exit. Expression is controlled by the ACE2 and SWI5 transcription factors.2 Publications

Gene expression databases

GenevestigatoriP38285.

Interactioni

Subunit structurei

Interacts with TEM1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TEM1P389876EBI-20853,EBI-19113

Protein-protein interaction databases

BioGridi32857. 42 interactions.
DIPiDIP-4930N.
IntActiP38285. 7 interactions.
MINTiMINT-550873.
STRINGi4932.YBR158W.

Structurei

3D structure databases

ProteinModelPortaliP38285.
SMRiP38285. Positions 338-383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AMN1 family.Curated

Phylogenomic databases

eggNOGiNOG275193.
HOGENOMiHOG000033921.
InParanoidiP38285.
KOiK15081.
OMAiAGCHITD.
OrthoDBiEOG78H42W.

Sequencei

Sequence statusi: Complete.

P38285-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLERVSSNG SFKRGRDIQS LESPCTRPLK KMSPSPSFTS LKMEKPFKDI
60 70 80 90 100
VRKYGGHLHQ SSYNPGSSKV ELVRPDLSLK TDQSFLQSSV QTTPNKKSCN
110 120 130 140 150
EYLSTPEATP LKNTATENAW ATSRVVSASS LSIVTPTEIK NILVDEFSEL
160 170 180 190 200
KLGQPLTAQH QRSHAVFEIP EIVENIIKMI VSLESANIPK ERPCLRRNPQ
210 220 230 240 250
SYEHSLLMYK DEERAKKAWS AAQQLRDPPL VGHKEKKQGA LFSCMMVNRL
260 270 280 290 300
WLNVTRPFLF KSLHFKSVHN FKEFLRTSQE TTQVMRPSHF ILHKLHQVTQ
310 320 330 340 350
PDIERLSRME CQNLKWLEFY VCPRITPPLS WFDNLHKLEK LIIPGNKNID
360 370 380 390 400
DNFLLRLSQS IPNLKHLVLR ACDNVSDSGV VCIALNCPKL KTFNIGRHRR
410 420 430 440 450
GNLITSVSLV ALGKYTQVET VGFAGCDVDD AGIWEFARLN GKNVERLSLN
460 470 480 490 500
SCRLLTDYSL PILFALNSFP NLAVLEIRNL DKITDVRHFV KYNLWKKSLD
510 520 530 540
APILIEACER ITKLIDQEEN RVKRINSLVA LKDMTAWVNA DDEIENNVD
Length:549
Mass (Da):62,686
Last modified:October 1, 1994 - v1
Checksum:iEA1EC675D6A84F20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36027 Genomic DNA. Translation: CAA85117.1.
AY723760 Genomic DNA. Translation: AAU09677.1.
BK006936 Genomic DNA. Translation: DAA07273.1.
PIRiS46029.
RefSeqiNP_009716.1. NM_001178506.1.

Genome annotation databases

EnsemblFungiiYBR158W; YBR158W; YBR158W.
GeneIDi852455.
KEGGisce:YBR158W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36027 Genomic DNA. Translation: CAA85117.1 .
AY723760 Genomic DNA. Translation: AAU09677.1 .
BK006936 Genomic DNA. Translation: DAA07273.1 .
PIRi S46029.
RefSeqi NP_009716.1. NM_001178506.1.

3D structure databases

ProteinModelPortali P38285.
SMRi P38285. Positions 338-383.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32857. 42 interactions.
DIPi DIP-4930N.
IntActi P38285. 7 interactions.
MINTi MINT-550873.
STRINGi 4932.YBR158W.

Proteomic databases

MaxQBi P38285.
PaxDbi P38285.
PeptideAtlasi P38285.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR158W ; YBR158W ; YBR158W .
GeneIDi 852455.
KEGGi sce:YBR158W.

Organism-specific databases

CYGDi YBR158w.
SGDi S000000362. AMN1.

Phylogenomic databases

eggNOGi NOG275193.
HOGENOMi HOG000033921.
InParanoidi P38285.
KOi K15081.
OMAi AGCHITD.
OrthoDBi EOG78H42W.

Enzyme and pathway databases

BioCyci YEAST:G3O-29108-MONOMER.

Miscellaneous databases

NextBioi 971381.

Gene expression databases

Genevestigatori P38285.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: FUNCTION.
  5. "New yeast genes important for chromosome integrity and segregation identified by dosage effects on genome stability."
    Ouspenski I.I., Elledge S.J., Brinkley B.R.
    Nucleic Acids Res. 27:3001-3008(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p."
    Doolin M.-T., Johnson A.L., Johnston L.H., Butler G.
    Mol. Microbiol. 40:422-432(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling."
    Wang Y., Shirogane T., Liu D., Harper J.W., Elledge S.J.
    Cell 112:697-709(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION, INTERACTION WITH TEM1.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Trans-acting regulatory variation in Saccharomyces cerevisiae and the role of transcription factors."
    Yvert G., Brem R.B., Whittle J., Akey J.M., Foss E., Smith E.N., Mackelprang R., Kruglyak L.
    Nat. Genet. 35:57-64(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Phosphatase 2A negatively regulates mitotic exit in Saccharomyces cerevisiae."
    Wang Y., Ng T.-Y.
    Mol. Biol. Cell 17:80-89(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiAMN1_YEAST
AccessioniPrimary (citable) accession number: P38285
Secondary accession number(s): D6VQF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 26, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2020 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3