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Protein

Antagonist of mitotic exit network protein 1

Gene

AMN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Negative regulator of the mitotic exit network (MEN), required for multiple cell cycle checkpoints. Acts in the daughter cell to inhibit the mitotic exit pathway once MEN has executed its function. Through its binding ability to TEM1, interferes with the TEM1-CDC5 association, required for CDC5 kinase activation and MEN activation. Required for daughter cell separation and chromosome stability. Involved in copper sensitivity.5 Publications

Miscellaneous

Present with 2020 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic cell cycle checkpoint Source: SGD
  • negative regulation of exit from mitosis Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-29108-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Antagonist of mitotic exit network protein 1
Alternative name(s):
Chromosome stability protein 13
Increased copper-sensitivity protein 4
Gene namesi
Name:AMN1
Synonyms:CST13, ICS4
Ordered Locus Names:YBR158W
ORF Names:YBR1208
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR158W
SGDiS000000362 AMN1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002025001 – 549Antagonist of mitotic exit network protein 1Add BLAST549

Proteomic databases

MaxQBiP38285
PaxDbiP38285
PRIDEiP38285

Expressioni

Inductioni

Expressed in daughter cells after execution of mitotic exit. Expression is controlled by the ACE2 and SWI5 transcription factors.2 Publications

Interactioni

Subunit structurei

Interacts with TEM1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TEM1P389876EBI-20853,EBI-19113

Protein-protein interaction databases

BioGridi32857, 71 interactors
DIPiDIP-4930N
IntActiP38285, 8 interactors
STRINGi4932.YBR158W

Structurei

3D structure databases

ProteinModelPortaliP38285
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AMN1 family.Curated

Phylogenomic databases

HOGENOMiHOG000033921
InParanoidiP38285
KOiK15081
OMAiAGCHITD
OrthoDBiEOG092C493Q

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR006553 Leu-rich_rpt_Cys-con_subtyp
IPR032675 LRR_dom_sf
SMARTiView protein in SMART
SM00367 LRR_CC, 5 hits

Sequencei

Sequence statusi: Complete.

P38285-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLERVSSNG SFKRGRDIQS LESPCTRPLK KMSPSPSFTS LKMEKPFKDI
60 70 80 90 100
VRKYGGHLHQ SSYNPGSSKV ELVRPDLSLK TDQSFLQSSV QTTPNKKSCN
110 120 130 140 150
EYLSTPEATP LKNTATENAW ATSRVVSASS LSIVTPTEIK NILVDEFSEL
160 170 180 190 200
KLGQPLTAQH QRSHAVFEIP EIVENIIKMI VSLESANIPK ERPCLRRNPQ
210 220 230 240 250
SYEHSLLMYK DEERAKKAWS AAQQLRDPPL VGHKEKKQGA LFSCMMVNRL
260 270 280 290 300
WLNVTRPFLF KSLHFKSVHN FKEFLRTSQE TTQVMRPSHF ILHKLHQVTQ
310 320 330 340 350
PDIERLSRME CQNLKWLEFY VCPRITPPLS WFDNLHKLEK LIIPGNKNID
360 370 380 390 400
DNFLLRLSQS IPNLKHLVLR ACDNVSDSGV VCIALNCPKL KTFNIGRHRR
410 420 430 440 450
GNLITSVSLV ALGKYTQVET VGFAGCDVDD AGIWEFARLN GKNVERLSLN
460 470 480 490 500
SCRLLTDYSL PILFALNSFP NLAVLEIRNL DKITDVRHFV KYNLWKKSLD
510 520 530 540
APILIEACER ITKLIDQEEN RVKRINSLVA LKDMTAWVNA DDEIENNVD
Length:549
Mass (Da):62,686
Last modified:October 1, 1994 - v1
Checksum:iEA1EC675D6A84F20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36027 Genomic DNA Translation: CAA85117.1
AY723760 Genomic DNA Translation: AAU09677.1
BK006936 Genomic DNA Translation: DAA07273.1
PIRiS46029
RefSeqiNP_009716.1, NM_001178506.1

Genome annotation databases

EnsemblFungiiYBR158W; YBR158W; YBR158W
GeneIDi852455
KEGGisce:YBR158W

Similar proteinsi

Entry informationi

Entry nameiAMN1_YEAST
AccessioniPrimary (citable) accession number: P38285
Secondary accession number(s): D6VQF3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 23, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names
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Main funding by: National Institutes of Health