P38283 (SLI15_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inner centromere protein-related protein SLI15
Short name=INCENP-related protein SLI15

Gene names

Name:	SLI15
Ordered Locus Names:	YBR156C
ORF Names:	YBR1206

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	698 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for high-fidelity chromosome segregation during the later part of each cell cycle in association with the protein kinase IPL1. Stimulates IPL1 kinase activity and facilitates its association with the mitotic spindle. Has a role in attaching the kinetochores to the microtubules and ensuring that sister kinetochores connect to opposite poles. Ref.5 Ref.6 Ref.7
Subcellular location	Nucleus. Cytoplasm › cytoskeleton › spindle. Chromosome › centromere › kinetochore. Note: Associates with the mitotic spindle and the kinetochore. Ref.5 Ref.6
Post-translational modification	Phosphorylated by serine/threonine protein kinase IPL1. Ref.6
Miscellaneous	Present with 319 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the INCENP family.

Ontologies

Keywords
Biological process	Chromosome partition
Cellular component	Centromere Chromosome Cytoplasm Cytoskeleton Kinetochore Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chromosome segregation Traceable author statement Ref.6. Source: SGD protein phosphorylation Inferred from direct assay PubMed 17504936. Source: SGD regulation of cytokinesis Inferred from mutant phenotype PubMed 16615892. Source: SGD
Cellular_component	chromosome passenger complex Inferred from direct assay PubMed 19158380. Source: SGD condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW kinetochore microtubule Inferred from direct assay Ref.6. Source: SGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell spindle midzone Inferred from direct assay PubMed 12566427. Source: SGD
Molecular_function	protein kinase activator activity Inferred from physical interaction Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC14	Q00684	2	EBI-20842,EBI-4192
CLB2	P24869	2	EBI-20842,EBI-4515
DAM1	P53267	2	EBI-20842,EBI-23268
IPL1	P38991	3	EBI-20842,EBI-9319

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 698	698	Inner centromere protein-related protein SLI15		PRO_0000071956
Amino acid modifications
	Modified residue	97	1	Phosphoserine Ref.10
	Modified residue	144	1	Phosphoserine Ref.10
	Modified residue	233	1	Phosphoserine Ref.9
	Modified residue	268	1	Phosphoserine Ref.9
	Modified residue	309	1	Phosphothreonine Ref.10
	Modified residue	329	1	Phosphoserine Ref.10
	Modified residue	489	1	Phosphoserine Ref.10
	Modified residue	554	1	Phosphoserine Ref.9
	Modified residue	649	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P38283 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 827AC2AB884BA7D1	FASTA	698	79,186
	10 20 30 40 50 60 MDWAIKAARK KTQRKPGSTR SIIETLDDLN NLTTDAHSEI NQRLYESSEW LRNNVYMNTL 70 80 90 100 110 120 KYEDKKMEES LISPENTHNK MDVEFPKMKG EYELSNSQND AAKDVTKTPR NGLHNDKSIT 130 140 150 160 170 180 PKSLRRKEVT EGMNRFSIHD TNKSPVEPLN SVKVDANESE KSSPWSPYKV EKVLRESSKT 190 200 210 220 230 240 SESPINTKRF DNQTWAAKEE MENEPILQAL KKAESVKVKP PPNSGIARSQ RRSNMFVPLP 250 260 270 280 290 300 NKDPLIIQHI PPTKSSGSIP KVRTVKESPI AFKKKSTINS PAIRAVENSD TAGSTKASSV 310 320 330 340 350 360 FDRLSSIPTK SFENKISRGN VGHKYSSSSI DLTGSPMKKV SQKFKSINST DTDMQEALRD 370 380 390 400 410 420 IFSVKNKITK NNSPKGKNSR KSSIPRFDKT SLKLTTHKKL AIIAEQKKKS KHSSDVHKTG 430 440 450 460 470 480 SRPHSISPTK ISVDSSSPSK EVKNYYQSPV RGYLRPTKAS ISPNKNKNLT TSQTPHRLKI 490 500 510 520 530 540 KEKTLRKLSP NIADISKPES RKSKNYRLTN LQLLPPAEAE RDDLKKKFDK RLSGIMRSQQ 550 560 570 580 590 600 EHHRRKQEKQ KRMSHLEQDL KKQTSFSNDY KDIRLKESLA PFDNHVRDTI NKNTAFSTDN 610 620 630 640 650 660 ILATINTVDH REIIGNVTPK IASVNDSLPE INTDSEDEAS VTLAAWAKSP YLQEQLIRQQ 670 680 690 DINPQTIFGP IPPLHTDEIF PNPRLNRLKP RQIVPKRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a 4.8 kb fragment of Saccharomyces cerevisiae chromosome II including three essential open reading frames." Baur A., Schaaff-Gerstenschlaeger I., Boles E., Miosga T., Rose M., Zimmermann F.K. Yeast 9:289-293(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Sequence and functional analysis of a 7.2 kb DNA fragment containing four open reading frames located between RPB5 and CDC28 on the right arm of chromosome II." Rose M., Kiesau P., Proft M., Entian K.-D. Yeast 11:865-871(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Sli15 associates with the ipl1 protein kinase to promote proper chromosome segregation in Saccharomyces cerevisiae." Kim J.-H., Kang J.-S., Chan C.S.M. J. Cell Biol. 145:1381-1394(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Functional cooperation of Dam1, Ipl1, and the inner centromere protein (INCENP)-related protein Sli15 during chromosome segregation." Kang J.-S., Cheeseman I.M., Kallstrom G., Velmurugan S., Barnes G., Chan C.S.M. J. Cell Biol. 155:763-774(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
[7]	"Evidence that the Ipl1-Sli15 (Aurora kinase-INCENP) complex promotes chromosome bi-orientation by altering kinetochore-spindle pole connections." Tanaka T.U., Rachidi N., Janke C., Pereira G., Galova M., Schiebel E., Stark M.J.R., Nasmyth K. Cell 108:317-329(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-233; SER-268 AND SER-554, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97; SER-144; THR-309; SER-329; SER-489 AND SER-649, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71329 Genomic DNA. No translation available. S59774 Genomic DNA. Translation: AAC60557.1. Z36025 Genomic DNA. Translation: CAA85115.1. BK006936 Genomic DNA. Translation: DAA07271.1.
PIR	S46027.
RefSeq	NP_009714.3. NM_001178504.3.
3D structure databases
ProteinModelPortal	P38283.
SMR	P38283. Positions 642-681.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5807N.
IntAct	P38283. 6 interactions.
MINT	MINT-2787312.
STRING	4932.YBR156C.
Proteomic databases
PaxDb	P38283.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR156C; YBR156C; YBR156C.
GeneID	852453.
KEGG	sce:YBR156C. sce:YBR160W.
Organism-specific databases
CYGD	YBR156c.
SGD	S000000360. SLI15.
Phylogenomic databases
eggNOG	NOG12793.
KO	K04563.
OMA	LHIDEIF.
OrthoDB	EOG40ZV6V.
Gene expression databases
Genevestigator	P38283.
GermOnline	YBR156C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005635. Inner_centromere_prot_ARK-bd. [Graphical view]
Pfam	PF03941. INCENP_ARK-bind. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971375.

Entry information

Entry name

SLI15_YEAST

Accession

Primary (citable) accession number: P38283
Secondary accession number(s): D6VQF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program