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Protein

25S rRNA (adenine(2142)-N(1))-methyltransferase

Gene

BMT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N1 position of adenine 2142 in 25S rRNA. N1-methyladenine(2142) in 25S rRNA is present in helix 65, a region that accounts for most of the intersubunit surface of the large subunit.UniRule annotation1 Publication

Miscellaneous

Present with 623 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + adenine(2142) in 25S rRNA = S-adenosyl-L-homocysteine + N1-methyladenine(2142) in 25S rRNA.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei180S-adenosyl-L-methionineUniRule annotation1
Binding sitei201S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

  • rRNA (adenine) methyltransferase activity Source: SGD
  • S-adenosylmethionine-dependent methyltransferase activity Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-29095-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
25S rRNA (adenine(2142)-N(1))-methyltransferaseUniRule annotation (EC:2.1.1.286UniRule annotation)
Alternative name(s):
Base MethylTransferase of 25S RNAUniRule annotation
Gene namesi
Name:BMT2UniRule annotation
Ordered Locus Names:YBR141C
ORF Names:YBR1118
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR141C
SGDiS000000345 BMT2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Loss of N1-methyladenine(2142) in 25S rRNA. Confers anisomycin and peroxide sensitivity to the cells as well as slight defects in ribosome subunit joining.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi180G → R: Abolishes methyltransferase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002024941 – 33725S rRNA (adenine(2142)-N(1))-methyltransferaseAdd BLAST337

Proteomic databases

MaxQBiP38278
PaxDbiP38278
PRIDEiP38278

Interactioni

Protein-protein interaction databases

BioGridi32841, 186 interactors
DIPiDIP-1766N
IntActiP38278, 2 interactors
MINTiP38278
STRINGi4932.YBR141C

Structurei

3D structure databases

ProteinModelPortaliP38278
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the BMT2 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000171862
InParanoidiP38278
KOiK18849
OMAiGGKMAYW
OrthoDBiEOG092C3SND

Family and domain databases

HAMAPiMF_03044 BMT2, 1 hit
InterProiView protein in InterPro
IPR021867 Bmt2/SAMTOR
IPR029063 SAM-dependent_MTases
PANTHERiPTHR21008 PTHR21008, 1 hit
PfamiView protein in Pfam
PF11968 Bmt2, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

P38278-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSRKSKSIT GKRKQVGSNV TRVIKPQKTR RIIRRFHHLI NKRQSICKFL
60 70 80 90 100
CLKENLDDSN EEKNDKIIRL SIKGNVRLGK YYEDGKSQSF NDAMESQLLR
110 120 130 140 150
LHSLIKNESK SKDTSDLAVM YTLLGYIMNQ INKLGGLETY QIASQNGQLK
160 170 180 190 200
ERGGDTSKLL EKWIRSSFEN CPGAVALEIG SLSSGNRISR CALFRNVVRI
210 220 230 240 250
DLEEHEGVIK QDFMERPLPR NENDKFDLIS CSLVLNFVKN HRDRGAMCHR
260 270 280 290 300
MVKFLKPQGY IFIVLPQACV THSRYCDKTL LQNLLGSIGL IMLNSHQSNK
310 320 330
LYYCLYQLQV VPPQPSSFSK RIKVNDGPGL NNFGITL
Length:337
Mass (Da):38,550
Last modified:July 27, 2011 - v2
Checksum:iCB0309828FABF3BE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti312P → S in CAA85099 (PubMed:7754712).Curated1
Sequence conflicti312P → S in CAA55538 (PubMed:7813418).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36010 Genomic DNA Translation: CAA85099.1
X78937 Genomic DNA Translation: CAA55538.1
BK006936 Genomic DNA Translation: DAA07257.2
PIRiS46010
RefSeqiNP_009699.2, NM_001178489.2

Genome annotation databases

EnsemblFungiiYBR141C; YBR141C; YBR141C
GeneIDi852438
KEGGisce:YBR141C

Similar proteinsi

Entry informationi

Entry nameiBMT2_YEAST
AccessioniPrimary (citable) accession number: P38278
Secondary accession number(s): D6VQD7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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