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Protein

25S rRNA (adenine(2142)-N(1))-methyltransferase

Gene

BMT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N1 position of adenine 2142 in 25S rRNA. N(1)-methyladenine(2142) in 25S rRNA is present in helix 65, a region that accounts for most of the intersubunit surface of the large subunit.UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + adenine(2142) in 25S rRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(2142) in 25S rRNA.UniRule annotation1 Publication

GO - Molecular functioni

  • rRNA (adenine) methyltransferase activity Source: SGD
  • S-adenosylmethionine-dependent methyltransferase activity Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-29095-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
25S rRNA (adenine(2142)-N(1))-methyltransferaseUniRule annotation (EC:2.1.1.286UniRule annotation)
Alternative name(s):
Base MethylTransferase of 25S RNAUniRule annotation
Gene namesi
Name:BMT2UniRule annotation
Ordered Locus Names:YBR141C
ORF Names:YBR1118
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR141C.
SGDiS000000345. BMT2.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Loss of N(1)-methyladenine(2142) in 25S rRNA. Confers anisomycin and peroxide sensitivity to the cells as well as slight defects in ribosome subunit joining.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi180G → R: Abolishes methyltransferase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002024941 – 33725S rRNA (adenine(2142)-N(1))-methyltransferaseAdd BLAST337

Proteomic databases

MaxQBiP38278.

Interactioni

Protein-protein interaction databases

BioGridi32841. 93 interactors.
DIPiDIP-1766N.
IntActiP38278. 2 interactors.
MINTiMINT-407203.

Structurei

3D structure databases

ProteinModelPortaliP38278.
SMRiP38278.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the BMT2 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000171862.
InParanoidiP38278.
KOiK18849.
OMAiIIRRFHL.
OrthoDBiEOG092C3SND.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03044. BMT2. 1 hit.
InterProiIPR021867. Bmt2.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF11968. Bmt2. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

P38278-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSRKSKSIT GKRKQVGSNV TRVIKPQKTR RIIRRFHHLI NKRQSICKFL
60 70 80 90 100
CLKENLDDSN EEKNDKIIRL SIKGNVRLGK YYEDGKSQSF NDAMESQLLR
110 120 130 140 150
LHSLIKNESK SKDTSDLAVM YTLLGYIMNQ INKLGGLETY QIASQNGQLK
160 170 180 190 200
ERGGDTSKLL EKWIRSSFEN CPGAVALEIG SLSSGNRISR CALFRNVVRI
210 220 230 240 250
DLEEHEGVIK QDFMERPLPR NENDKFDLIS CSLVLNFVKN HRDRGAMCHR
260 270 280 290 300
MVKFLKPQGY IFIVLPQACV THSRYCDKTL LQNLLGSIGL IMLNSHQSNK
310 320 330
LYYCLYQLQV VPPQPSSFSK RIKVNDGPGL NNFGITL
Length:337
Mass (Da):38,550
Last modified:July 27, 2011 - v2
Checksum:iCB0309828FABF3BE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti312P → S in CAA85099 (PubMed:7754712).Curated1
Sequence conflicti312P → S in CAA55538 (PubMed:7813418).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36010 Genomic DNA. Translation: CAA85099.1.
X78937 Genomic DNA. Translation: CAA55538.1.
BK006936 Genomic DNA. Translation: DAA07257.2.
PIRiS46010.
RefSeqiNP_009699.2. NM_001178489.2.

Genome annotation databases

EnsemblFungiiYBR141C; YBR141C; YBR141C.
GeneIDi852438.
KEGGisce:YBR141C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36010 Genomic DNA. Translation: CAA85099.1.
X78937 Genomic DNA. Translation: CAA55538.1.
BK006936 Genomic DNA. Translation: DAA07257.2.
PIRiS46010.
RefSeqiNP_009699.2. NM_001178489.2.

3D structure databases

ProteinModelPortaliP38278.
SMRiP38278.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32841. 93 interactors.
DIPiDIP-1766N.
IntActiP38278. 2 interactors.
MINTiMINT-407203.

Proteomic databases

MaxQBiP38278.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR141C; YBR141C; YBR141C.
GeneIDi852438.
KEGGisce:YBR141C.

Organism-specific databases

EuPathDBiFungiDB:YBR141C.
SGDiS000000345. BMT2.

Phylogenomic databases

HOGENOMiHOG000171862.
InParanoidiP38278.
KOiK18849.
OMAiIIRRFHL.
OrthoDBiEOG092C3SND.

Enzyme and pathway databases

BioCyciYEAST:G3O-29095-MONOMER.

Miscellaneous databases

PROiP38278.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03044. BMT2. 1 hit.
InterProiIPR021867. Bmt2.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF11968. Bmt2. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBMT2_YEAST
AccessioniPrimary (citable) accession number: P38278
Secondary accession number(s): D6VQD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 623 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.