Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

SWI5-dependent HO expression protein 3

Gene

SHE3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Required for the delivery of cortical endoplasmic reticulum into the emerging bud.11 Publications

GO - Molecular functioni

  • mRNA binding Source: SGD

GO - Biological processi

  • endoplasmic reticulum inheritance Source: SGD
  • intracellular mRNA localization Source: SGD
  • mating type switching Source: SGD
  • mRNA transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29085-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
SWI5-dependent HO expression protein 3
Gene namesi
Name:SHE3
Ordered Locus Names:YBR130C
ORF Names:YBR1005
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR130C.
SGDiS000000334. SHE3.

Subcellular locationi

GO - Cellular componenti

  • cellular bud tip Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi343S → E: Prevents correct localization of ASH1 mRNA; when associated with E-361. 1 Publication1
Mutagenesisi348S → E: Prevents correct localization of ASH1 mRNA. 1 Publication1
Mutagenesisi361S → E: Prevents correct localization of ASH1 mRNA; when associated with E-343. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002024901 – 425SWI5-dependent HO expression protein 3Add BLAST425

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei343PhosphoserineCombined sources1
Modified residuei394PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38272.
PRIDEiP38272.

PTM databases

iPTMnetiP38272.

Interactioni

Subunit structurei

Interacts with SHE2 and MYO4.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MYO4P324927EBI-21600,EBI-11681
SHE2P360683EBI-21600,EBI-26866

Protein-protein interaction databases

BioGridi32831. 66 interactors.
DIPiDIP-2037N.
IntActiP38272. 18 interactors.
MINTiMINT-572712.

Structurei

Secondary structure

1425
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi44 – 71Combined sources28
Helixi74 – 134Combined sources61

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LL7X-ray2.31A/B/C/D/E/F/G/H42-137[»]
4LL8X-ray3.58B/E81-311[»]
4WNLX-ray2.80E/F/G/H342-374[»]
ProteinModelPortaliP38272.
SMRiP38272.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili68 – 197Sequence analysisAdd BLAST130

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi377 – 381Poly-Ser5

Sequence similaritiesi

Belongs to the SHE3 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

InParanoidiP38272.
KOiK18737.
OMAiSMLNRKT.
OrthoDBiEOG092C5AYK.

Family and domain databases

InterProiIPR031398. She3.
[Graphical view]
PfamiPF17078. SHE3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38272-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDQDNTQTS SSKLAPHHNI FMANLESSPT KDRNTSSQNA SSSRVIESLH
60 70 80 90 100
DQIDMLTKTN LQLTTQSQNL LSKLELAQSK ESKLLENLNL LKNENENLNS
110 120 130 140 150
IFERKNKKLK ELEKDYSELS NRYNEQKEKM DQLSKLAKNS SAIEQSCSEK
160 170 180 190 200
LQNMEVNYNS LLESQNLYRD HYSDEISKLN EKIGLLELEL SNQNLNYGSD
210 220 230 240 250
TSSNSDIELN LNKFNDSVKD LKSLETEKDS KLSKIITHSL DELNLQSWLN
260 270 280 290 300
LYQTNENLIS TFAEKMDLKD VLKRNDEKIS NKGAVVQTLK KNVQTQVESN
310 320 330 340 350
NADALSSNNA QDMLPIKMVK LRKTPNTNDS SSNGNSSNNK RRSFYTASPL
360 370 380 390 400
LSSGSIPKSA SPVLPGVKRT ASVRKPSSSS SKTNVTHNND PSTSPTISVP
410 420
PGVTRTVSST HKKKGNSMVV HGAQS
Length:425
Mass (Da):47,417
Last modified:October 1, 1994 - v1
Checksum:i590404D2E140BB7A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75891 Genomic DNA. Translation: CAA53489.1.
Z35999 Genomic DNA. Translation: CAA85087.1.
BK006936 Genomic DNA. Translation: DAA07247.1.
PIRiS45999.
RefSeqiNP_009688.3. NM_001178478.3.

Genome annotation databases

EnsemblFungiiYBR130C; YBR130C; YBR130C.
GeneIDi852427.
KEGGisce:YBR130C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75891 Genomic DNA. Translation: CAA53489.1.
Z35999 Genomic DNA. Translation: CAA85087.1.
BK006936 Genomic DNA. Translation: DAA07247.1.
PIRiS45999.
RefSeqiNP_009688.3. NM_001178478.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LL7X-ray2.31A/B/C/D/E/F/G/H42-137[»]
4LL8X-ray3.58B/E81-311[»]
4WNLX-ray2.80E/F/G/H342-374[»]
ProteinModelPortaliP38272.
SMRiP38272.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32831. 66 interactors.
DIPiDIP-2037N.
IntActiP38272. 18 interactors.
MINTiMINT-572712.

PTM databases

iPTMnetiP38272.

Proteomic databases

MaxQBiP38272.
PRIDEiP38272.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR130C; YBR130C; YBR130C.
GeneIDi852427.
KEGGisce:YBR130C.

Organism-specific databases

EuPathDBiFungiDB:YBR130C.
SGDiS000000334. SHE3.

Phylogenomic databases

InParanoidiP38272.
KOiK18737.
OMAiSMLNRKT.
OrthoDBiEOG092C5AYK.

Enzyme and pathway databases

BioCyciYEAST:G3O-29085-MONOMER.

Miscellaneous databases

PROiP38272.

Family and domain databases

InterProiIPR031398. She3.
[Graphical view]
PfamiPF17078. SHE3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSHE3_YEAST
AccessioniPrimary (citable) accession number: P38272
Secondary accession number(s): D6VQC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1010 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.