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P38272

- SHE3_YEAST

UniProt

P38272 - SHE3_YEAST

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Protein

SWI5-dependent HO expression protein 3

Gene

SHE3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Required for the delivery of cortical endoplasmic reticulum into the emerging bud.11 Publications

GO - Molecular functioni

  1. mRNA binding Source: SGD

GO - Biological processi

  1. endoplasmic reticulum inheritance Source: SGD
  2. intracellular mRNA localization Source: SGD
  3. mating type switching Source: SGD
  4. mRNA transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29085-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
SWI5-dependent HO expression protein 3
Gene namesi
Name:SHE3
Ordered Locus Names:YBR130C
ORF Names:YBR1005
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR130c.
SGDiS000000334. SHE3.

Subcellular locationi

Endoplasmic reticulum membrane 2 Publications; Peripheral membrane protein 2 Publications

GO - Cellular componenti

  1. cellular bud tip Source: SGD
  2. endoplasmic reticulum Source: UniProtKB-KW
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi343 – 3431S → E: Prevents correct localization of ASH1 mRNA; when associated with E-361. 1 Publication
Mutagenesisi348 – 3481S → E: Prevents correct localization of ASH1 mRNA. 1 Publication
Mutagenesisi361 – 3611S → E: Prevents correct localization of ASH1 mRNA; when associated with E-343. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 425425SWI5-dependent HO expression protein 3PRO_0000202490Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei343 – 3431Phosphoserine1 Publication
Modified residuei394 – 3941Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38272.
PaxDbiP38272.
PeptideAtlasiP38272.
PRIDEiP38272.

Expressioni

Gene expression databases

GenevestigatoriP38272.

Interactioni

Subunit structurei

Interacts with SHE2 and MYO4.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MYO4P324927EBI-21600,EBI-11681
SHE2P360683EBI-21600,EBI-26866

Protein-protein interaction databases

BioGridi32831. 63 interactions.
DIPiDIP-2037N.
IntActiP38272. 17 interactions.
MINTiMINT-572712.
STRINGi4932.YBR130C.

Structurei

Secondary structure

1
425
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi44 – 7128
Helixi74 – 13461

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LL7X-ray2.31A/B/C/D/E/F/G/H42-137[»]
4LL8X-ray3.58B/E81-311[»]
ProteinModelPortaliP38272.
SMRiP38272. Positions 43-196.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili68 – 197130Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi377 – 3815Poly-Ser

Sequence similaritiesi

Belongs to the SHE3 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG130615.
InParanoidiP38272.
OMAiFMANLES.
OrthoDBiEOG7J44J8.

Sequencei

Sequence statusi: Complete.

P38272-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDQDNTQTS SSKLAPHHNI FMANLESSPT KDRNTSSQNA SSSRVIESLH
60 70 80 90 100
DQIDMLTKTN LQLTTQSQNL LSKLELAQSK ESKLLENLNL LKNENENLNS
110 120 130 140 150
IFERKNKKLK ELEKDYSELS NRYNEQKEKM DQLSKLAKNS SAIEQSCSEK
160 170 180 190 200
LQNMEVNYNS LLESQNLYRD HYSDEISKLN EKIGLLELEL SNQNLNYGSD
210 220 230 240 250
TSSNSDIELN LNKFNDSVKD LKSLETEKDS KLSKIITHSL DELNLQSWLN
260 270 280 290 300
LYQTNENLIS TFAEKMDLKD VLKRNDEKIS NKGAVVQTLK KNVQTQVESN
310 320 330 340 350
NADALSSNNA QDMLPIKMVK LRKTPNTNDS SSNGNSSNNK RRSFYTASPL
360 370 380 390 400
LSSGSIPKSA SPVLPGVKRT ASVRKPSSSS SKTNVTHNND PSTSPTISVP
410 420
PGVTRTVSST HKKKGNSMVV HGAQS
Length:425
Mass (Da):47,417
Last modified:October 1, 1994 - v1
Checksum:i590404D2E140BB7A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75891 Genomic DNA. Translation: CAA53489.1.
Z35999 Genomic DNA. Translation: CAA85087.1.
BK006936 Genomic DNA. Translation: DAA07247.1.
PIRiS45999.
RefSeqiNP_009688.3. NM_001178478.3.

Genome annotation databases

EnsemblFungiiYBR130C; YBR130C; YBR130C.
GeneIDi852427.
KEGGisce:YBR130C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75891 Genomic DNA. Translation: CAA53489.1 .
Z35999 Genomic DNA. Translation: CAA85087.1 .
BK006936 Genomic DNA. Translation: DAA07247.1 .
PIRi S45999.
RefSeqi NP_009688.3. NM_001178478.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4LL7 X-ray 2.31 A/B/C/D/E/F/G/H 42-137 [» ]
4LL8 X-ray 3.58 B/E 81-311 [» ]
ProteinModelPortali P38272.
SMRi P38272. Positions 43-196.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32831. 63 interactions.
DIPi DIP-2037N.
IntActi P38272. 17 interactions.
MINTi MINT-572712.
STRINGi 4932.YBR130C.

Proteomic databases

MaxQBi P38272.
PaxDbi P38272.
PeptideAtlasi P38272.
PRIDEi P38272.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR130C ; YBR130C ; YBR130C .
GeneIDi 852427.
KEGGi sce:YBR130C.

Organism-specific databases

CYGDi YBR130c.
SGDi S000000334. SHE3.

Phylogenomic databases

eggNOGi NOG130615.
InParanoidi P38272.
OMAi FMANLES.
OrthoDBi EOG7J44J8.

Enzyme and pathway databases

BioCyci YEAST:G3O-29085-MONOMER.

Miscellaneous databases

NextBioi 971303.

Gene expression databases

Genevestigatori P38272.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of 29.7 kb from the right arm of chromosome II reveals 13 complete open reading frames, of which ten correspond to new genes."
    Becam A.-M., Cullin C., Grzybowska E., Lacroute F., Nasr F., Ozier-Kalogeropoulos O., Palucha A., Slonimski P.P., Zagulski M., Herbert C.J.
    Yeast 10:S1-S11(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: FUNCTION.
  5. "Localization and anchoring of mRNA in budding yeast."
    Beach D.L., Salmon E.D., Bloom K.
    Curr. Biol. 9:569-578(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Association of the class V myosin Myo4p with a localised messenger RNA in budding yeast depends on She proteins."
    Munchow S., Sauter C., Jansen R.P.
    J. Cell Sci. 112:1511-1518(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "She2p, a novel RNA-binding protein tethers ASH1 mRNA to the Myo4p myosin motor via She3p."
    Bohl F., Kruse C., Frank A., Ferring D., Jansen R.P.
    EMBO J. 19:5514-5524(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SHE2 AND MYO4.
  8. "She2p is a novel RNA-binding protein that recruits the Myo4p-She3p complex to ASH1 mRNA."
    Long R.M., Gu W., Lorimer E., Singer R.H., Chartrand P.
    EMBO J. 19:6592-6601(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, RNA-BINDING, INTERACTION WITH SHE2.
  9. "The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p."
    Takizawa P.A., Vale R.D.
    Proc. Natl. Acad. Sci. U.S.A. 97:5273-5278(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA-BINDING, INTERACTION WITH MYO4.
  10. "Ribonucleoprotein-dependent localization of the yeast class V myosin Myo4p."
    Kruse C., Jaedicke A., Beaudouin J., Bohl F., Ferring D., Guttler T., Ellenberg J., Jansen R.P.
    J. Cell Biol. 159:971-982(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Myo4p and She3p are required for cortical ER inheritance in Saccharomyces cerevisiae."
    Estrada P., Kim J., Coleman J., Walker L., Dunn B., Takizawa P., Novick P., Ferro-Novick S.
    J. Cell Biol. 163:1255-1266(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  13. "RNA-protein interactions promote asymmetric sorting of the ASH1 mRNA ribonucleoprotein complex."
    Gonsalvez G.B., Lehmann K.A., Ho D.K., Stanitsa E.S., Williamson J.R., Long R.M.
    RNA 9:1383-1399(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SHE2.
  14. "ASH1 mRNA anchoring requires reorganization of the Myo4p-She3p-She2p transport complex."
    Gonsalvez G.B., Little J.L., Long R.M.
    J. Biol. Chem. 279:46286-46294(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. "Coordination of endoplasmic reticulum and mRNA localization to the yeast bud."
    Schmid M., Jaedicke A., Du T.G., Jansen R.P.
    Curr. Biol. 16:1538-1543(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  16. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-343, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  17. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  18. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-394, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  19. "She3p possesses a novel activity required for ASH1 mRNA localization in Saccharomyces cerevisiae."
    Landers S.M., Gallas M.R., Little J., Long R.M.
    Eukaryot. Cell 8:1072-1083(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, MUTAGENESIS OF SER-343; SER-348 AND SER-361.
  20. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-394, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  21. "The structure of the Myo4p globular tail and its function in ASH1 mRNA localization."
    Heuck A., Fetka I., Brewer D.N., Huls D., Munson M., Jansen R.P., Niessing D.
    J. Cell Biol. 189:497-510(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MYO4.

Entry informationi

Entry nameiSHE3_YEAST
AccessioniPrimary (citable) accession number: P38272
Secondary accession number(s): D6VQC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 29, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1010 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3