P38270 (ATG14_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Autophagy-related protein 14

Alternative name(s):
Cytoplasm to vacuole targeting protein 12

Gene names

Name:	ATG14
Synonyms:	APG14, CVT12
Ordered Locus Names:	YBR128C
ORF Names:	YBR1003

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Ref.2 Ref.7 Ref.8 Ref.9 Ref.10
Subunit structure	Component of the autophagy-specific VPS34 PI3-kinase complex I composed of VPS15, VPS30, VPS34 and ATG14.
Subcellular location	Membrane; Peripheral membrane protein Ref.2 Ref.10.
Sequence similarities	Belongs to the ATG14 family.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Membrane
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CVT pathway Inferred from mutant phenotype PubMed 8901576. Source: SGD ER-associated protein catabolic process Inferred from mutant phenotype PubMed 16267277. Source: SGD macroautophagy Inferred from mutant phenotype Ref.10Ref.2. Source: SGD peroxisome degradation Inferred from mutant phenotype PubMed 21121900. Source: SGD phosphatidylinositol-3-phosphate biosynthetic process Inferred from physical interaction PubMed 16421251. Source: GOC piecemeal microautophagy of nucleus Inferred from mutant phenotype PubMed 18701704. Source: SGD positive regulation of autophagy Inferred from electronic annotation. Source: InterPro
Cellular_component	extrinsic to membrane Inferred from direct assay Ref.2. Source: SGD fungal-type vacuole membrane Inferred from direct assay PubMed 16421251. Source: SGD phosphatidylinositol 3-kinase complex I Inferred from direct assay Ref.10. Source: SGD pre-autophagosomal structure Inferred from direct assay PubMed 16421251. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
VPS30	Q02948	7	EBI-2699,EBI-2670

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Autophagy-related protein 14		PRO_0000212453
Regions
	Coiled coil	25 – 156	132	Potential

Sequences

	Sequence		Length	Mass (Da)
	P38270 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 202039BC7EC8BB16	FASTA	344	40,454
	10 20 30 40 50 60 MHCPICHHRA HVVYCAHCIN TSPSLLLKLK LDLILLKDEN KELNGKVEQI LNEAMNYDQL 70 80 90 100 110 120 DIKRMEKKKD PLMNSLMKLD VLRMKKNNNL IRHRIEQLNE RIYSKRNHIS ELKVEIDNYK 130 140 150 160 170 180 CYKVGTGTDK LREQVEISDA KNKLAQVSKI CESARDYKLN LLNNWFVIQK LQDNFQIPFA 190 200 210 220 230 240 IAFQPLISLK NFRILPLAIT NDSINIMWKY ISFFSDILMI KLPYTNKICE QPMFEFSDSI 250 260 270 280 290 300 QTVVQRLIKL IINILQICRH LKLVPSTPMD IPWLLDQYDV DGLFYNMVKR NKMKCRSVSL 310 320 330 340 YWTFGMLYSM VLDNMNNPQR GHPARRTAPP PTVTGPHDRW YVVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of 29.7 kb from the right arm of chromosome II reveals 13 complete open reading frames, of which ten correspond to new genes." Becam A.-M., Cullin C., Grzybowska E., Lacroute F., Nasr F., Ozier-Kalogeropoulos O., Palucha A., Slonimski P.P., Zagulski M., Herbert C.J. Yeast 10:S1-S11(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast, Saccharomyces cerevisiae." Kametaka S., Okano T., Ohsumi M., Ohsumi Y. J. Biol. Chem. 273:22284-22291(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VPS30.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"A conserved gene encoding the 57-kDa subunit of the yeast vacuolar H+-ATPase." Nelson H., Mandiyan S., Nelson N. J. Biol. Chem. 264:1775-1778(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 207-344.
[7]	"Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae." Tsukada M., Ohsumi Y. FEBS Lett. 333:169-174(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway." Harding T.M., Hefner-Gravink A., Thumm M., Klionsky D.J. J. Biol. Chem. 271:17621-17624(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation." Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y. EMBO J. 20:5971-5981(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae." Kihara A., Noda T., Ishihara N., Ohsumi Y. J. Cell Biol. 152:519-530(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VPS15; VPS30 AND VPS34.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X75891 Genomic DNA. Translation: CAA53487.1. Z35997 Genomic DNA. Translation: CAA85085.1. AB011071 Genomic DNA. Translation: BAA32103.1. AY693167 Genomic DNA. Translation: AAT93186.1. J04450 Genomic DNA. Translation: AAA66889.1. BK006936 Genomic DNA. Translation: DAA07245.1.
PIR	S45997.
RefSeq	NP_009686.1. NM_001178476.1.
3D structure databases
ProteinModelPortal	P38270.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2741N.
IntAct	P38270. 6 interactions.
MINT	MINT-486362.
STRING	4932.YBR128C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR128C; YBR128C; YBR128C.
GeneID	852425.
KEGG	sce:YBR128C.
Organism-specific databases
CYGD	YBR128c.
SGD	S000000332. ATG14.
Phylogenomic databases
eggNOG	NOG39057.
HOGENOM	HOG000034132.
KO	K08335.
OMA	NDSINIM.
OrthoDB	EOG48D44Z.
Gene expression databases
Genevestigator	P38270.
GermOnline	YBR128C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR023261. Autophagy-related_protein_14. IPR018791. UV_resistance/autophagy_Atg14. [Graphical view]
Pfam	PF10186. Atg14. 1 hit. [Graphical view]
PRINTS	PR02030. AUTOPHGYRP14.
ProDom	PD871282. Autophagy-related_protein_14. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other
NextBio	971297.

Entry information

Entry name

ATG14_YEAST

Accession

Primary (citable) accession number: P38270
Secondary accession number(s): D6VQC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program