P38266 (AIM3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Altered inheritance of mitochondria protein 3

Gene names

Name:	AIM3
Ordered Locus Names:	YBR108W
ORF Names:	YBR0901

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	947 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with RVS167. Ref.5
Subcellular location	Membrane raft; Peripheral membrane protein. Note: Localize within detergent-insoluble glycolipid-enriched membranes. Ref.5
Disruption phenotype	Increases frequency of mitochondrial genome loss. Ref.10
Sequence similarities	Belongs to the AIM3 family.
Sequence caution	The sequence CAA55611.1 differs from that shown. Reason: Frameshift at position 841. The sequence CAA85063.1 differs from that shown. Reason: Frameshift at position 841.

Ontologies

Keywords
Cellular component	Membrane
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	actin cortical patch assembly Inferred from genetic interaction. Source: SGD
Cellular component	actin cortical patch Inferred from direct assay. Source: SGD membrane raft Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LSB3	P43603	3	EBI-21584,EBI-22980
RVS167	P39743	5	EBI-21584,EBI-14500
YSC84	P32793	3	EBI-21584,EBI-24460

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 947	947	Altered inheritance of mitochondria protein 3		PRO_0000202486
Regions
	Compositional bias	95 – 398	304	Gln-rich
	Compositional bias	371 – 447	77	Pro-rich
	Compositional bias	862 – 907	46	Pro-rich
Amino acid modifications
	Modified residue	57	1	Phosphoserine Ref.9
	Modified residue	58	1	Phosphoserine Ref.9
	Modified residue	64	1	Phosphoserine Ref.9
	Modified residue	476	1	Phosphoserine Ref.9
	Modified residue	669	1	Phosphoserine Ref.8
	Modified residue	729	1	Phosphothreonine Ref.7 Ref.9
	Modified residue	805	1	Phosphothreonine Ref.6 Ref.8
	Modified residue	826	1	Phosphothreonine Ref.7
	Modified residue	842	1	Phosphoserine Ref.8 Ref.9
	Modified residue	843	1	Phosphoserine Ref.8 Ref.9
	Modified residue	847	1	Phosphoserine Ref.9
	Modified residue	861	1	Phosphothreonine Ref.9
	Modified residue	914	1	Phosphotyrosine Ref.9
	Modified residue	915	1	Phosphoserine Ref.6 Ref.9
	Modified residue	919	1	Phosphoserine Ref.9
Experimental info
	Sequence conflict	515	1	V → A in CAA55611. Ref.1
	Sequence conflict	515	1	V → A in CAA85063. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P38266 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: CD624428B29DA272	FASTA	947	103,863
	10 20 30 40 50 60 MGFWENNKDS ITSGLKSAGK YGYQGTKYVA KTGYKASKKH YNNSKARRER KSGKKNSSDE 70 80 90 100 110 120 EYDSEDEMEY ERKPTDIRSL KDPKSFPPPP LKPGQKTYTG QQQQQMPNGQ ASYAFQGAYQ 130 140 150 160 170 180 GQPGAGSTEQ SQYAQPQYNQ YPQQQLQQGV MPQQQQLQQG VVPQQPPIYG EQVPPYGSNS 190 200 210 220 230 240 NATSYQSLPQ QNQPQNAIPS QVSLNSASQQ STGFVSQNLQ YGTQSSNPAP SPSFQNGLQC 250 260 270 280 290 300 HQQPQYVSHG STNLGQSQFP SGQQQQPTTQ FGQQVLPSPA QPQQQQQGQP LPPPRGQVIL 310 320 330 340 350 360 PAPGEPLSNG FGQQQQQQQQ QQQPLNQNNA LLPQMNVEGV SGMAAVQPVY GQAMSSTTNM 370 380 390 400 410 420 QDSNPSYGAS PMQGQPPVGG QPPVPVRMQP QPPQPMQQGN IYPIEPSLDS TGSTPHFEVT 430 440 450 460 470 480 PFDPDAPAPK PKIDIPTVDV SSLPPPPTHR DRGAVVHQEP APSGKIQPNT TSSAASLPAK 490 500 510 520 530 540 HSRTTTADNE RNSGNKENDE STSKSSILGH YDVDVNIMPP PKPFRHGLDS VPSEHTTKNA 550 560 570 580 590 600 PERAVPILPP RNNVEPPPPP SRGNFERTES VLSTNAANVQ EDPISNFLPP PKPFRHTETK 610 620 630 640 650 660 QNQNSKASPV EMKGEVLPGH PSEEDRNVEP SLVPQSKPQS QSQFRRAHME TQPIQNFQPP 670 680 690 700 710 720 PKPFRRSQSS NSSDSSYTID GPEANHGRGR GRIAKHHDGD EYNPKSENST ENGRLGDAPN 730 740 750 760 770 780 SFIRKRAPTP PAPSRSEKLH EGTITSEVDS SKDANKYEKS IPPVTSSIQA QQSTKKAPPP 790 800 810 820 830 840 VVKPKPRNFS LKANEYPKEL TREATGQDEV LNSITNELSH IKLRKTNVNL EKLGGSKKVK 850 860 870 880 890 900 DSSPVPSDLD EKYVSASGSI TPPRPPPSRS SPKKVPPVVP KKNDNLKKKP PVVPKKKPLL 910 920 930 940 KSLEPRPIEM ERAYSGDISA ADDNLNPFER YKRNVVPQED DRLHKLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 70 kb region on the right arm of yeast chromosome II." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 10:1363-1381(1994) [PubMed: 7900426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 515. Strain: ATCC 204508 / S288c.
[4]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFT.
[5]	"Characterizing the sphingolipid signaling pathway that remediates defects associated with loss of the yeast amphiphysin-like orthologs, Rvs161p and Rvs167p." Germann M., Swain E., Bergman L., Nickels J.T. Jr. J. Biol. Chem. 280:4270-4278(2005) [PubMed: 15561700] [Abstract] Cited for: INTERACTION WITH RVS167, SUBCELLULAR LOCATION.
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-805 AND SER-915, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-729 AND THR-826, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-669; THR-805; SER-842 AND SER-843, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57; SER-58; SER-64; SER-476; THR-729; SER-842; SER-843; SER-847; THR-861; TYR-914; SER-915 AND SER-919, MASS SPECTROMETRY.
[10]	"Computationally driven, quantitative experiments discover genes required for mitochondrial biogenesis." Hess D.C., Myers C.L., Huttenhower C., Hibbs M.A., Hayes A.P., Paw J., Clore J.J., Mendoza R.M., Luis B.S., Nislow C., Giaever G., Costanzo M., Troyanskaya O.G., Caudy A.A. PLoS Genet. 5:E1000407-E1000407(2009) [PubMed: 19300474] [Abstract] Cited for: DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78993 Genomic DNA. Translation: CAA55611.1. Frameshift. Z35977 Genomic DNA. Translation: CAA85063.1. Frameshift. BK006936 Genomic DNA. Translation: DAA07227.2.
PIR	S48273.
RefSeq	NP_009666.3. NM_001178456.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1602N.
IntAct	P38266. 6 interactions.
MINT	MINT-385431.
STRING	P38266.
Proteomic databases
PeptideAtlas	P38266.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	852405.
KEGG	sce:YBR108W.
NMPDR	fig|4932.3.peg.365.
Organism-specific databases
CYGD	YBR108w.
SGD	S000000312. AIM3.
Phylogenomic databases
eggNOG	fuNOG11984.
OMA	GECWDLA.
OrthoDB	EOG4B0205.
Gene expression databases
ArrayExpress	P38266.
Genevestigator	P38266.
GermOnline	YBR108W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	971246.

Entry information

Entry name

AIM3_YEAST

Accession

Primary (citable) accession number: P38266
Secondary accession number(s): D6VQA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	July 27, 2011
Last modified:	January 25, 2012
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program