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Protein

SIR4-interacting protein SIF2

Gene

SIF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Antagonizes telomeric silencing in yeast. May recruit SIR4 to non-telomeric sites or repression.

GO - Molecular functioni

GO - Biological processi

  • histone deacetylation Source: SGD
  • negative regulation of chromatin silencing at telomere Source: SGD
  • negative regulation of meiotic nuclear division Source: SGD
  • positive regulation of stress-activated MAPK cascade Source: SGD
  • regulation of transcription, DNA-templated Source: SGD
  • regulation of transcription from RNA polymerase II promoter Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Enzyme and pathway databases

BioCyciYEAST:G3O-29065-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
SIR4-interacting protein SIF2
Gene namesi
Name:SIF2
Synonyms:EMB1
Ordered Locus Names:YBR103W
ORF Names:YBR0832
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR103W.
SGDiS000000307. SIF2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
  • Set3 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000512161 – 535SIR4-interacting protein SIF2Add BLAST535

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei137PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38262.
PRIDEiP38262.

PTM databases

iPTMnetiP38262.

Interactioni

Subunit structurei

Homotetramer. Interacts with SIR4 N-terminal domain. Interacts with a complex composed of SIN3 and RPD3. Identified in the Set3C complex with HOS2, HST1, SNT1, CPR1, HOS4/YIL112W and SET3.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HOS4P404804EBI-17136,EBI-8492
HST1P536852EBI-17136,EBI-8691

Protein-protein interaction databases

BioGridi32807. 221 interactors.
DIPiDIP-1377N.
IntActiP38262. 20 interactors.
MINTiMINT-391284.

Structurei

Secondary structure

1535
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi152 – 156Combined sources5
Beta strandi161 – 165Combined sources5
Beta strandi172 – 177Combined sources6
Beta strandi181 – 189Combined sources9
Beta strandi198 – 207Combined sources10
Beta strandi223 – 228Combined sources6
Beta strandi232 – 239Combined sources8
Beta strandi244 – 248Combined sources5
Beta strandi253 – 257Combined sources5
Beta strandi264 – 269Combined sources6
Beta strandi273 – 280Combined sources8
Beta strandi285 – 289Combined sources5
Turni290 – 293Combined sources4
Beta strandi294 – 299Combined sources6
Beta strandi325 – 328Combined sources4
Beta strandi331 – 335Combined sources5
Helixi337 – 339Combined sources3
Beta strandi341 – 345Combined sources5
Beta strandi352 – 355Combined sources4
Beta strandi362 – 368Combined sources7
Turni369 – 372Combined sources4
Beta strandi373 – 378Combined sources6
Beta strandi383 – 386Combined sources4
Beta strandi388 – 392Combined sources5
Beta strandi394 – 397Combined sources4
Beta strandi404 – 410Combined sources7
Turni411 – 413Combined sources3
Beta strandi414 – 419Combined sources6
Beta strandi422 – 428Combined sources7
Turni429 – 432Combined sources4
Beta strandi433 – 439Combined sources7
Beta strandi445 – 450Combined sources6
Beta strandi454 – 461Combined sources8
Beta strandi466 – 470Combined sources5
Helixi472 – 475Combined sources4
Beta strandi496 – 500Combined sources5
Beta strandi509 – 514Combined sources6
Beta strandi518 – 528Combined sources11
Beta strandi531 – 534Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R5MX-ray1.55A113-535[»]
ProteinModelPortaliP38262.
SMRiP38262.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38262.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 36LisHPROSITE-ProRule annotationAdd BLAST33
Repeati155 – 186WD 1Add BLAST32
Repeati218 – 248WD 2Add BLAST31
Repeati259 – 289WD 3Add BLAST31
Repeati316 – 345WD 4Add BLAST30
Repeati357 – 387WD 5Add BLAST31
Repeati399 – 428WD 6Add BLAST30
Repeati440 – 470WD 7Add BLAST31
Repeati503 – 534WD 8Add BLAST32

Domaini

The LisH domain mediates tetramerization and interaction with SNT1.1 Publication

Sequence similaritiesi

Contains 1 LisH domain.PROSITE-ProRule annotation
Contains 8 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00860000133748.
HOGENOMiHOG000111162.
InParanoidiP38262.
KOiK04508.
OMAiNENATHF.
OrthoDBiEOG092C28XH.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR006594. LisH.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08513. LisH. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00667. LisH. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50896. LISH. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38262-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSITSEELNY LIWRYCQEMG HEVSALALQD ETRVLEFDEK YKEHIPLGTL
60 70 80 90 100
VNLVQRGILY TESELMVDSK GDISALNEHH LSEDFNLVQA LQIDKEKFPE
110 120 130 140 150
ISSEGRFTLE TNSESNKAGE DGASTVERET QEDDTNSIDS SDDLDGFVKI
160 170 180 190 200
LKEIVKLDNI VSSTWNPLDE SILAYGEKNS VARLARIVET DQEGKKYWKL
210 220 230 240 250
TIIAELRHPF ALSASSGKTT NQVTCLAWSH DGNSIVTGVE NGELRLWNKT
260 270 280 290 300
GALLNVLNFH RAPIVSVKWN KDGTHIISMD VENVTILWNV ISGTVMQHFE
310 320 330 340 350
LKETGGSSIN AENHSGDGSL GVDVEWVDDD KFVIPGPKGA IFVYQITEKT
360 370 380 390 400
PTGKLIGHHG PISVLEFNDT NKLLLSASDD GTLRIWHGGN GNSQNCFYGH
410 420 430 440 450
SQSIVSASWV GDDKVISCSM DGSVRLWSLK QNTLLALSIV DGVPIFAGRI
460 470 480 490 500
SQDGQKYAVA FMDGQVNVYD LKKLNSKSRS LYGNRDGILN PLPIPLYASY
510 520 530
QSSQDNDYIF DLSWNCAGNK ISVAYSLQEG SVVAI
Length:535
Mass (Da):59,161
Last modified:October 25, 2004 - v2
Checksum:i25C0AEA2CCEF1B07
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti396C → S in CAA55606 (PubMed:7900426).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78993 Genomic DNA. Translation: CAA55606.1.
Z35972 Genomic DNA. Translation: CAA85058.1.
AY693053 Genomic DNA. Translation: AAT93072.1.
BK006936 Genomic DNA. Translation: DAA07222.1.
PIRiS48268.
RefSeqiNP_009661.1. NM_001178451.1.

Genome annotation databases

EnsemblFungiiYBR103W; YBR103W; YBR103W.
GeneIDi852399.
KEGGisce:YBR103W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78993 Genomic DNA. Translation: CAA55606.1.
Z35972 Genomic DNA. Translation: CAA85058.1.
AY693053 Genomic DNA. Translation: AAT93072.1.
BK006936 Genomic DNA. Translation: DAA07222.1.
PIRiS48268.
RefSeqiNP_009661.1. NM_001178451.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R5MX-ray1.55A113-535[»]
ProteinModelPortaliP38262.
SMRiP38262.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32807. 221 interactors.
DIPiDIP-1377N.
IntActiP38262. 20 interactors.
MINTiMINT-391284.

PTM databases

iPTMnetiP38262.

Proteomic databases

MaxQBiP38262.
PRIDEiP38262.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR103W; YBR103W; YBR103W.
GeneIDi852399.
KEGGisce:YBR103W.

Organism-specific databases

EuPathDBiFungiDB:YBR103W.
SGDiS000000307. SIF2.

Phylogenomic databases

GeneTreeiENSGT00860000133748.
HOGENOMiHOG000111162.
InParanoidiP38262.
KOiK04508.
OMAiNENATHF.
OrthoDBiEOG092C28XH.

Enzyme and pathway databases

BioCyciYEAST:G3O-29065-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP38262.
PROiP38262.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR006594. LisH.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08513. LisH. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00667. LisH. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50896. LISH. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSIF2_YEAST
AccessioniPrimary (citable) accession number: P38262
Secondary accession number(s): D6VQA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 25, 2004
Last modified: November 30, 2016
This is version 161 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1620 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.