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P38261

- EXO84_YEAST

UniProt

P38261 - EXO84_YEAST

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Protein
Exocyst complex component EXO84
Gene
EXO84, USA3, YBR102C, YBR0831
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Plays a role in both the assembly of the exocyst and the polarization of this complex to specific sites of the plasma membrane for exocytosis and to the budding site. Also involved in assembly of the spliceosome.3 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. Golgi to plasma membrane transport Source: SGD
  2. exocyst assembly Source: SGD
  3. exocyst localization Source: SGD
  4. exocytosis Source: SGD
  5. protein transport Source: UniProtKB-KW
  6. spliceosomal complex assembly Source: SGD
  7. vesicle tethering involved in exocytosis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29064-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Exocyst complex component EXO84
Alternative name(s):
Exocyst complex protein of 84 kDa
U1 SNP1-associating protein 3
Gene namesi
Name:EXO84
Synonyms:USA3
Ordered Locus Names:YBR102C
ORF Names:YBR0831
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR102c.
SGDiS000000306. EXO84.

Subcellular locationi

Cytoplasmic vesiclesecretory vesicle. Bud. Bud neck
Note: Cell periphery, bud and bud neck. The polarization of EXO84 requires actin cables.4 Publications

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cellular bud tip Source: SGD
  3. exocyst Source: SGD
  4. incipient cellular bud site Source: SGD
  5. transport vesicle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 753753Exocyst complex component EXO84
PRO_0000118987Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581Phosphothreonine1 Publication
Modified residuei139 – 1391Phosphoserine1 Publication
Cross-linki219 – 219Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei482 – 4821Phosphoserine1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP38261.
PaxDbiP38261.
PeptideAtlasiP38261.
PRIDEiP38261.

Expressioni

Gene expression databases

GenevestigatoriP38261.

Interactioni

Subunit structurei

Component of the exocyst complex composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84. Interacts also with SNP1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SEC10Q062456EBI-21567,EBI-16504
SEC15P222243EBI-21567,EBI-16543
SEC5P891026EBI-21567,EBI-16865
SEC8P328555EBI-21567,EBI-16896
SNP1Q009162EBI-21567,EBI-724

Protein-protein interaction databases

BioGridi32806. 58 interactions.
DIPiDIP-2732N.
IntActiP38261. 19 interactions.
MINTiMINT-617970.
STRINGi4932.YBR102C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi528 – 54821
Helixi552 – 56817
Helixi579 – 60426
Helixi609 – 62113
Helixi625 – 64521
Helixi653 – 67826
Helixi684 – 70825
Helixi717 – 72812
Helixi729 – 7335
Helixi738 – 7469
Helixi747 – 7515

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D2SX-ray2.85A523-753[»]
ProteinModelPortaliP38261.
SMRiP38261. Positions 525-753.

Miscellaneous databases

EvolutionaryTraceiP38261.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili227 – 27953 Reviewed prediction
Add
BLAST
Coiled coili531 – 58555 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the EXO84 family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG321873.
HOGENOMiHOG000000727.
OMAiVRANIND.
OrthoDBiEOG7CG77P.

Family and domain databases

InterProiIPR016159. Cullin_repeat-like_dom.
[Graphical view]
SUPFAMiSSF74788. SSF74788. 1 hit.

Sequencei

Sequence statusi: Complete.

P38261-1 [UniParc]FASTAAdd to Basket

« Hide

MVEFSLKKAR NNWKHVKKSA SSPAKQKTPP SPAKPKQKTK KNPYSDLKDP    50
ATSYTLPTIN ARERSRVATS MQRRLSIHNT NYAPPTLDYS MPLPDMPNMI 100
VPNDNVDSSH NNSSFTTENE SVSSKGPSNS LNLSTADLSL NDSSYNKVPA 150
RSAMRNTVNP SGSNDPFNNS TSLRKMLANP HFNAKDFVHD KLGNASAITI 200
DKFTSNLTDL SIQVQEEVKL NINKSYNEIM TVNNDLNVAM LELKRVRANI 250
NDLNEVLDQC TKIAEKRLQL QDQIDQERQG NFNNVESHSN SPALLPPLKA 300
GQNGNLMRRD RSSVLILEKF WDTELDQLFK NVEGAQKFIN STKGRHILMN 350
SANWMELNTT TGKPLQMVQI FILNDLVLIA DKSRDKQNDF IVSQCYPLKD 400
VTVTQEEFST KRLLFKFSNS NSSLYECRDA DECSRLLDVI RKAKDDLCDI 450
FHVEEENSKR IRESFRYLQS TQQTPGRENN RSPNKNKRRS MGGSITPGRN 500
VTGAMDQYLL QNLTLSMHSR PRSRDMSSTA QRLKFLDEGV EEIDIELARL 550
RFESAVETLL DIESQLEDLS ERISDEELML LNLISLKIEQ RREAISSKLS 600
QSILSSNEIV HLKSGTENMI KLGLPEQALD LFLQNRSNFI QDLILQIGSV 650
DNPTNYLTQL AVIRFQTIKK TVEDFQDIFK ELGAKISSIL VDWCSDEVDN 700
HFKLIDKQLL NDEMLSPGSI KSSRKQIDGL KAVGLDFVYK LDEFIKKNSD 750
KIR 753
Length:753
Mass (Da):85,538
Last modified:October 1, 1994 - v1
Checksum:i1BCDE834466C3536
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78993 Genomic DNA. Translation: CAA55605.1.
Z35971 Genomic DNA. Translation: CAA85057.1.
BK006936 Genomic DNA. Translation: DAA07221.1.
PIRiS48267.
RefSeqiNP_009660.1. NM_001178450.1.

Genome annotation databases

EnsemblFungiiYBR102C; YBR102C; YBR102C.
GeneIDi852398.
KEGGisce:YBR102C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78993 Genomic DNA. Translation: CAA55605.1 .
Z35971 Genomic DNA. Translation: CAA85057.1 .
BK006936 Genomic DNA. Translation: DAA07221.1 .
PIRi S48267.
RefSeqi NP_009660.1. NM_001178450.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2D2S X-ray 2.85 A 523-753 [» ]
ProteinModelPortali P38261.
SMRi P38261. Positions 525-753.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32806. 58 interactions.
DIPi DIP-2732N.
IntActi P38261. 19 interactions.
MINTi MINT-617970.
STRINGi 4932.YBR102C.

Proteomic databases

MaxQBi P38261.
PaxDbi P38261.
PeptideAtlasi P38261.
PRIDEi P38261.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR102C ; YBR102C ; YBR102C .
GeneIDi 852398.
KEGGi sce:YBR102C.

Organism-specific databases

CYGDi YBR102c.
SGDi S000000306. EXO84.

Phylogenomic databases

eggNOGi NOG321873.
HOGENOMi HOG000000727.
OMAi VRANIND.
OrthoDBi EOG7CG77P.

Enzyme and pathway databases

BioCyci YEAST:G3O-29064-MONOMER.

Miscellaneous databases

EvolutionaryTracei P38261.
NextBioi 971228.
PROi P38261.

Gene expression databases

Genevestigatori P38261.

Family and domain databases

InterProi IPR016159. Cullin_repeat-like_dom.
[Graphical view ]
SUPFAMi SSF74788. SSF74788. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
    Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
    Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Exo84p is an exocyst protein essential for secretion."
    Guo W., Grant A., Novick P.
    J. Biol. Chem. 274:23558-23564(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SEC5; SEC8 AND SEC10, SUBCELLULAR LOCATION.
  5. "New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing."
    Awasthi S., Palmer R., Castro M., Mobarak C.D., Ruby S.W.
    J. Biol. Chem. 276:31004-31015(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SNP1.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-219.
    Strain: SUB592.
  8. "Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p."
    Boyd C., Hughes T., Pypaert M., Novick P.
    J. Cell Biol. 167:889-901(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. "The critical role of EXO84P in the organization and polarized localization of the exocyst complex."
    Zhang X., Zajac A., Zhang J., Wang P., Li M., Murray J., TerBush D.R., Guo W.
    J. Biol. Chem. 280:20356-20364(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  10. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-482, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-58, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif."
    Dong G., Hutagalung A.H., Fu C., Novick P., Reinisch K.M.
    Nat. Struct. Mol. Biol. 12:1094-1100(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 523-753.

Entry informationi

Entry nameiEXO84_YEAST
AccessioniPrimary (citable) accession number: P38261
Secondary accession number(s): D6VQA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 11, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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