Exocyst complex component EXO84 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38261 (EXO84_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 106. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Exocyst complex component EXO84

Alternative name(s):
Exocyst complex protein of 84 kDa
U1 SNP1-associating protein 3

Gene names

Name:	EXO84
Synonyms:	USA3
Ordered Locus Names:	YBR102C
ORF Names:	YBR0831

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	753 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Plays a role in both the assembly of the exocyst and the polarization of this complex to specific sites of the plasma membrane for exocytosis and to the budding site. Also involved in assembly of the spliceosome. Ref.5 Ref.6 Ref.9
Subunit structure	Component of the exocyst complex composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84. Interacts also with SNP1. Ref.5 Ref.6
Subcellular location	Cytoplasmic vesicle › secretory vesicle. Bud. Bud neck. Note: Cell periphery, bud and bud neck. The polarization of EXO84 requires actin cables. Ref.5 Ref.7 Ref.8 Ref.9
Sequence similarities	Belongs to the EXO84 family.

Ontologies

Keywords
Biological process	Exocytosis Protein transport Transport
Cellular component	Cytoplasmic vesicle
Domain	Coiled coil
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to plasma membrane transport Inferred from mutant phenotype Ref.5 Ref.9. Source: SGD exocyst assembly Inferred from mutant phenotype Ref.9. Source: SGD exocyst localization Inferred from mutant phenotype Ref.9. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW spliceosomal complex assembly Inferred from mutant phenotype Ref.6. Source: SGD vesicle tethering involved in exocytosis Inferred by curator Ref.5. Source: SGD
Cellular_component	cellular bud neck Inferred from direct assay Ref.5. Source: SGD cellular bud tip Inferred from direct assay Ref.5. Source: SGD exocyst Inferred from direct assay Ref.5. Source: SGD incipient cellular bud site Inferred from direct assay Ref.5. Source: SGD mating projection tip Inferred from direct assay PubMed 19053807. Source: SGD transport vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
SEC10	Q06245	6	EBI-21567,EBI-16504
SEC15	P22224	3	EBI-21567,EBI-16543
SEC5	P89102	6	EBI-21567,EBI-16865
SEC8	P32855	5	EBI-21567,EBI-16896
SNP1	Q00916	2	EBI-21567,EBI-724

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 753

753

Exocyst complex component EXO84

PRO_0000118987

Regions

Coiled coil

227 – 279

Potential

Coiled coil

531 – 585

Potential

Amino acid modifications

Modified residue

Phosphothreonine Ref.11

Modified residue

Phosphoserine Ref.11

Modified residue

130

Phosphoserine Ref.11 Ref.12

Modified residue

134

Phosphoserine Ref.11

Modified residue

139

Phosphoserine Ref.12

Modified residue

143

Phosphoserine Ref.12

Modified residue

470

Phosphoserine Ref.10

Modified residue

482

Phosphoserine Ref.10

Modified residue

490

Phosphoserine Ref.12

Modified residue

716

Phosphoserine Ref.12

Cross-link

219

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.4

Secondary structure

753

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P38261 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 1BCDE834466C3536
Show »

FASTA

753

85,538

        10         20         30         40         50         60 
MVEFSLKKAR NNWKHVKKSA SSPAKQKTPP SPAKPKQKTK KNPYSDLKDP ATSYTLPTIN 

        70         80         90        100        110        120 
ARERSRVATS MQRRLSIHNT NYAPPTLDYS MPLPDMPNMI VPNDNVDSSH NNSSFTTENE 

       130        140        150        160        170        180 
SVSSKGPSNS LNLSTADLSL NDSSYNKVPA RSAMRNTVNP SGSNDPFNNS TSLRKMLANP 

       190        200        210        220        230        240 
HFNAKDFVHD KLGNASAITI DKFTSNLTDL SIQVQEEVKL NINKSYNEIM TVNNDLNVAM 

       250        260        270        280        290        300 
LELKRVRANI NDLNEVLDQC TKIAEKRLQL QDQIDQERQG NFNNVESHSN SPALLPPLKA 

       310        320        330        340        350        360 
GQNGNLMRRD RSSVLILEKF WDTELDQLFK NVEGAQKFIN STKGRHILMN SANWMELNTT 

       370        380        390        400        410        420 
TGKPLQMVQI FILNDLVLIA DKSRDKQNDF IVSQCYPLKD VTVTQEEFST KRLLFKFSNS 

       430        440        450        460        470        480 
NSSLYECRDA DECSRLLDVI RKAKDDLCDI FHVEEENSKR IRESFRYLQS TQQTPGRENN 

       490        500        510        520        530        540 
RSPNKNKRRS MGGSITPGRN VTGAMDQYLL QNLTLSMHSR PRSRDMSSTA QRLKFLDEGV 

       550        560        570        580        590        600 
EEIDIELARL RFESAVETLL DIESQLEDLS ERISDEELML LNLISLKIEQ RREAISSKLS 

       610        620        630        640        650        660 
QSILSSNEIV HLKSGTENMI KLGLPEQALD LFLQNRSNFI QDLILQIGSV DNPTNYLTQL 

       670        680        690        700        710        720 
AVIRFQTIKK TVEDFQDIFK ELGAKISSIL VDWCSDEVDN HFKLIDKQLL NDEMLSPGSI 

       730        740        750 
KSSRKQIDGL KAVGLDFVYK LDEFIKKNSD KIR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 70 kb region on the right arm of yeast chromosome II." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 213-224, MASS SPECTROMETRY, UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-219. Strain: SUB592.
[5]	"Exo84p is an exocyst protein essential for secretion." Guo W., Grant A., Novick P. J. Biol. Chem. 274:23558-23564(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SEC5; SEC8 AND SEC10, SUBCELLULAR LOCATION.
[6]	"New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing." Awasthi S., Palmer R., Castro M., Mobarak C.D., Ruby S.W. J. Biol. Chem. 276:31004-31015(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SNP1.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p." Boyd C., Hughes T., Pypaert M., Novick P. J. Cell Biol. 167:889-901(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[9]	"The critical role of EXO84P in the organization and polarized localization of the exocyst complex." Zhang X., Zajac A., Zhang J., Wang P., Li M., Murray J., TerBush D.R., Guo W. J. Biol. Chem. 280:20356-20364(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-470 AND SER-482, MASS SPECTROMETRY.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-28; SER-31; SER-130 AND SER-134, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130; SER-139; SER-143; SER-490 AND SER-716, MASS SPECTROMETRY.
[13]	"The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif." Dong G., Hutagalung A.H., Fu C., Novick P., Reinisch K.M. Nat. Struct. Mol. Biol. 12:1094-1100(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 523-753.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X78993 Genomic DNA. Translation: CAA55605.1.
Z35971 Genomic DNA. Translation: CAA85057.1.
BK006936 Genomic DNA. Translation: DAA07221.1.

PIR

S48267.

RefSeq

NP_009660.1. NM_001178450.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2D2S	X-ray	2.85	A	523-753	[»]

ProteinModelPortal

P38261.

SMR

P38261. Positions 525-753.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2732N.

IntAct

P38261. 18 interactions.

MINT

MINT-617970.

STRING

4932.YBR102C.

Proteomic databases

PaxDb

P38261.

PeptideAtlas

P38261.

PRIDE

P38261.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YBR102C; YBR102C; YBR102C.

GeneID

852398.

KEGG

sce:YBR102C.

Organism-specific databases

CYGD

YBR102c.

SGD

S000000306. EXO84.

Phylogenomic databases

eggNOG

NOG321873.

HOGENOM

HOG000000727.

OMA

NINQNQK.

OrthoDB

EOG4B01ZC.

Gene expression databases

Genevestigator

P38261.

GermOnline

YBR102C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR016159. Cullin_repeat-like_dom.
IPR014812. Vps51.
[Graphical view]

Pfam

PF08700. Vps51. 1 hit.
[Graphical view]

SUPFAM

SSF74788. Cullin_repeat-like. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P38261.

NextBio

971228.

Entry information

Entry name

EXO84_YEAST

Accession

Primary (citable) accession number: P38261
Secondary accession number(s): D6VQA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents