Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P38261

- EXO84_YEAST

UniProt

P38261 - EXO84_YEAST

Protein

Exocyst complex component EXO84

Gene

EXO84

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Plays a role in both the assembly of the exocyst and the polarization of this complex to specific sites of the plasma membrane for exocytosis and to the budding site. Also involved in assembly of the spliceosome.3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. exocyst assembly Source: SGD
    2. exocyst localization Source: SGD
    3. exocytosis Source: SGD
    4. Golgi to plasma membrane transport Source: SGD
    5. protein transport Source: UniProtKB-KW
    6. spliceosomal complex assembly Source: SGD
    7. vesicle tethering involved in exocytosis Source: SGD

    Keywords - Biological processi

    Exocytosis, Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29064-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exocyst complex component EXO84
    Alternative name(s):
    Exocyst complex protein of 84 kDa
    U1 SNP1-associating protein 3
    Gene namesi
    Name:EXO84
    Synonyms:USA3
    Ordered Locus Names:YBR102C
    ORF Names:YBR0831
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR102c.
    SGDiS000000306. EXO84.

    Subcellular locationi

    Cytoplasmic vesiclesecretory vesicle. Bud. Bud neck
    Note: Cell periphery, bud and bud neck. The polarization of EXO84 requires actin cables.

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. cellular bud tip Source: SGD
    3. exocyst Source: SGD
    4. incipient cellular bud site Source: SGD
    5. transport vesicle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasmic vesicle

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 753753Exocyst complex component EXO84PRO_0000118987Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei58 – 581Phosphothreonine1 Publication
    Modified residuei139 – 1391Phosphoserine1 Publication
    Cross-linki219 – 219Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Modified residuei482 – 4821Phosphoserine1 Publication

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP38261.
    PaxDbiP38261.
    PeptideAtlasiP38261.
    PRIDEiP38261.

    Expressioni

    Gene expression databases

    GenevestigatoriP38261.

    Interactioni

    Subunit structurei

    Component of the exocyst complex composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84. Interacts also with SNP1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SEC10Q062456EBI-21567,EBI-16504
    SEC15P222243EBI-21567,EBI-16543
    SEC5P891026EBI-21567,EBI-16865
    SEC8P328555EBI-21567,EBI-16896
    SNP1Q009162EBI-21567,EBI-724

    Protein-protein interaction databases

    BioGridi32806. 58 interactions.
    DIPiDIP-2732N.
    IntActiP38261. 19 interactions.
    MINTiMINT-617970.
    STRINGi4932.YBR102C.

    Structurei

    Secondary structure

    1
    753
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi528 – 54821
    Helixi552 – 56817
    Helixi579 – 60426
    Helixi609 – 62113
    Helixi625 – 64521
    Helixi653 – 67826
    Helixi684 – 70825
    Helixi717 – 72812
    Helixi729 – 7335
    Helixi738 – 7469
    Helixi747 – 7515

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2D2SX-ray2.85A523-753[»]
    ProteinModelPortaliP38261.
    SMRiP38261. Positions 525-753.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38261.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili227 – 27953Sequence AnalysisAdd
    BLAST
    Coiled coili531 – 58555Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the EXO84 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG321873.
    HOGENOMiHOG000000727.
    OMAiVRANIND.
    OrthoDBiEOG7CG77P.

    Family and domain databases

    InterProiIPR016159. Cullin_repeat-like_dom.
    [Graphical view]
    SUPFAMiSSF74788. SSF74788. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P38261-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVEFSLKKAR NNWKHVKKSA SSPAKQKTPP SPAKPKQKTK KNPYSDLKDP    50
    ATSYTLPTIN ARERSRVATS MQRRLSIHNT NYAPPTLDYS MPLPDMPNMI 100
    VPNDNVDSSH NNSSFTTENE SVSSKGPSNS LNLSTADLSL NDSSYNKVPA 150
    RSAMRNTVNP SGSNDPFNNS TSLRKMLANP HFNAKDFVHD KLGNASAITI 200
    DKFTSNLTDL SIQVQEEVKL NINKSYNEIM TVNNDLNVAM LELKRVRANI 250
    NDLNEVLDQC TKIAEKRLQL QDQIDQERQG NFNNVESHSN SPALLPPLKA 300
    GQNGNLMRRD RSSVLILEKF WDTELDQLFK NVEGAQKFIN STKGRHILMN 350
    SANWMELNTT TGKPLQMVQI FILNDLVLIA DKSRDKQNDF IVSQCYPLKD 400
    VTVTQEEFST KRLLFKFSNS NSSLYECRDA DECSRLLDVI RKAKDDLCDI 450
    FHVEEENSKR IRESFRYLQS TQQTPGRENN RSPNKNKRRS MGGSITPGRN 500
    VTGAMDQYLL QNLTLSMHSR PRSRDMSSTA QRLKFLDEGV EEIDIELARL 550
    RFESAVETLL DIESQLEDLS ERISDEELML LNLISLKIEQ RREAISSKLS 600
    QSILSSNEIV HLKSGTENMI KLGLPEQALD LFLQNRSNFI QDLILQIGSV 650
    DNPTNYLTQL AVIRFQTIKK TVEDFQDIFK ELGAKISSIL VDWCSDEVDN 700
    HFKLIDKQLL NDEMLSPGSI KSSRKQIDGL KAVGLDFVYK LDEFIKKNSD 750
    KIR 753
    Length:753
    Mass (Da):85,538
    Last modified:October 1, 1994 - v1
    Checksum:i1BCDE834466C3536
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78993 Genomic DNA. Translation: CAA55605.1.
    Z35971 Genomic DNA. Translation: CAA85057.1.
    BK006936 Genomic DNA. Translation: DAA07221.1.
    PIRiS48267.
    RefSeqiNP_009660.1. NM_001178450.1.

    Genome annotation databases

    EnsemblFungiiYBR102C; YBR102C; YBR102C.
    GeneIDi852398.
    KEGGisce:YBR102C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78993 Genomic DNA. Translation: CAA55605.1 .
    Z35971 Genomic DNA. Translation: CAA85057.1 .
    BK006936 Genomic DNA. Translation: DAA07221.1 .
    PIRi S48267.
    RefSeqi NP_009660.1. NM_001178450.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2D2S X-ray 2.85 A 523-753 [» ]
    ProteinModelPortali P38261.
    SMRi P38261. Positions 525-753.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32806. 58 interactions.
    DIPi DIP-2732N.
    IntActi P38261. 19 interactions.
    MINTi MINT-617970.
    STRINGi 4932.YBR102C.

    Proteomic databases

    MaxQBi P38261.
    PaxDbi P38261.
    PeptideAtlasi P38261.
    PRIDEi P38261.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR102C ; YBR102C ; YBR102C .
    GeneIDi 852398.
    KEGGi sce:YBR102C.

    Organism-specific databases

    CYGDi YBR102c.
    SGDi S000000306. EXO84.

    Phylogenomic databases

    eggNOGi NOG321873.
    HOGENOMi HOG000000727.
    OMAi VRANIND.
    OrthoDBi EOG7CG77P.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29064-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P38261.
    NextBioi 971228.
    PROi P38261.

    Gene expression databases

    Genevestigatori P38261.

    Family and domain databases

    InterProi IPR016159. Cullin_repeat-like_dom.
    [Graphical view ]
    SUPFAMi SSF74788. SSF74788. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
      Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
      Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Exo84p is an exocyst protein essential for secretion."
      Guo W., Grant A., Novick P.
      J. Biol. Chem. 274:23558-23564(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SEC5; SEC8 AND SEC10, SUBCELLULAR LOCATION.
    5. "New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing."
      Awasthi S., Palmer R., Castro M., Mobarak C.D., Ruby S.W.
      J. Biol. Chem. 276:31004-31015(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SNP1.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-219.
      Strain: SUB592.
    8. "Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p."
      Boyd C., Hughes T., Pypaert M., Novick P.
      J. Cell Biol. 167:889-901(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    9. "The critical role of EXO84P in the organization and polarized localization of the exocyst complex."
      Zhang X., Zajac A., Zhang J., Wang P., Li M., Murray J., TerBush D.R., Guo W.
      J. Biol. Chem. 280:20356-20364(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    10. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-482, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-58, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif."
      Dong G., Hutagalung A.H., Fu C., Novick P., Reinisch K.M.
      Nat. Struct. Mol. Biol. 12:1094-1100(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 523-753.

    Entry informationi

    Entry nameiEXO84_YEAST
    AccessioniPrimary (citable) accession number: P38261
    Secondary accession number(s): D6VQA1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3