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P38261 (EXO84_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exocyst complex component EXO84
Alternative name(s):
Exocyst complex protein of 84 kDa
U1 SNP1-associating protein 3
Gene names
Name:EXO84
Synonyms:USA3
Ordered Locus Names:YBR102C
ORF Names:YBR0831
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length753 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Plays a role in both the assembly of the exocyst and the polarization of this complex to specific sites of the plasma membrane for exocytosis and to the budding site. Also involved in assembly of the spliceosome. Ref.4 Ref.5 Ref.9

Subunit structure

Component of the exocyst complex composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84. Interacts also with SNP1. Ref.4 Ref.5

Subcellular location

Cytoplasmic vesiclesecretory vesicle. Bud. Bud neck. Note: Cell periphery, bud and bud neck. The polarization of EXO84 requires actin cables. Ref.4 Ref.6 Ref.8 Ref.9

Sequence similarities

Belongs to the EXO84 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 753753Exocyst complex component EXO84
PRO_0000118987

Regions

Coiled coil227 – 27953 Potential
Coiled coil531 – 58555 Potential

Amino acid modifications

Modified residue581Phosphothreonine Ref.12
Modified residue1391Phosphoserine Ref.11
Modified residue4821Phosphoserine Ref.10
Cross-link219Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.7

Secondary structure

..................... 753
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P38261 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 1BCDE834466C3536

FASTA75385,538
        10         20         30         40         50         60 
MVEFSLKKAR NNWKHVKKSA SSPAKQKTPP SPAKPKQKTK KNPYSDLKDP ATSYTLPTIN 

        70         80         90        100        110        120 
ARERSRVATS MQRRLSIHNT NYAPPTLDYS MPLPDMPNMI VPNDNVDSSH NNSSFTTENE 

       130        140        150        160        170        180 
SVSSKGPSNS LNLSTADLSL NDSSYNKVPA RSAMRNTVNP SGSNDPFNNS TSLRKMLANP 

       190        200        210        220        230        240 
HFNAKDFVHD KLGNASAITI DKFTSNLTDL SIQVQEEVKL NINKSYNEIM TVNNDLNVAM 

       250        260        270        280        290        300 
LELKRVRANI NDLNEVLDQC TKIAEKRLQL QDQIDQERQG NFNNVESHSN SPALLPPLKA 

       310        320        330        340        350        360 
GQNGNLMRRD RSSVLILEKF WDTELDQLFK NVEGAQKFIN STKGRHILMN SANWMELNTT 

       370        380        390        400        410        420 
TGKPLQMVQI FILNDLVLIA DKSRDKQNDF IVSQCYPLKD VTVTQEEFST KRLLFKFSNS 

       430        440        450        460        470        480 
NSSLYECRDA DECSRLLDVI RKAKDDLCDI FHVEEENSKR IRESFRYLQS TQQTPGRENN 

       490        500        510        520        530        540 
RSPNKNKRRS MGGSITPGRN VTGAMDQYLL QNLTLSMHSR PRSRDMSSTA QRLKFLDEGV 

       550        560        570        580        590        600 
EEIDIELARL RFESAVETLL DIESQLEDLS ERISDEELML LNLISLKIEQ RREAISSKLS 

       610        620        630        640        650        660 
QSILSSNEIV HLKSGTENMI KLGLPEQALD LFLQNRSNFI QDLILQIGSV DNPTNYLTQL 

       670        680        690        700        710        720 
AVIRFQTIKK TVEDFQDIFK ELGAKISSIL VDWCSDEVDN HFKLIDKQLL NDEMLSPGSI 

       730        740        750 
KSSRKQIDGL KAVGLDFVYK LDEFIKKNSD KIR 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of a 70 kb region on the right arm of yeast chromosome II."
Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Exo84p is an exocyst protein essential for secretion."
Guo W., Grant A., Novick P.
J. Biol. Chem. 274:23558-23564(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SEC5; SEC8 AND SEC10, SUBCELLULAR LOCATION.
[5]"New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing."
Awasthi S., Palmer R., Castro M., Mobarak C.D., Ruby S.W.
J. Biol. Chem. 276:31004-31015(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SNP1.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"A proteomics approach to understanding protein ubiquitination."
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.
Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-219.
Strain: SUB592.
[8]"Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p."
Boyd C., Hughes T., Pypaert M., Novick P.
J. Cell Biol. 167:889-901(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"The critical role of EXO84P in the organization and polarized localization of the exocyst complex."
Zhang X., Zajac A., Zhang J., Wang P., Li M., Murray J., TerBush D.R., Guo W.
J. Biol. Chem. 280:20356-20364(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-482, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-58, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif."
Dong G., Hutagalung A.H., Fu C., Novick P., Reinisch K.M.
Nat. Struct. Mol. Biol. 12:1094-1100(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 523-753.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X78993 Genomic DNA. Translation: CAA55605.1.
Z35971 Genomic DNA. Translation: CAA85057.1.
BK006936 Genomic DNA. Translation: DAA07221.1.
PIRS48267.
RefSeqNP_009660.1. NM_001178450.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2D2SX-ray2.85A523-753[»]
ProteinModelPortalP38261.
SMRP38261. Positions 525-753.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32806. 58 interactions.
DIPDIP-2732N.
IntActP38261. 19 interactions.
MINTMINT-617970.
STRING4932.YBR102C.

Proteomic databases

MaxQBP38261.
PaxDbP38261.
PeptideAtlasP38261.
PRIDEP38261.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR102C; YBR102C; YBR102C.
GeneID852398.
KEGGsce:YBR102C.

Organism-specific databases

CYGDYBR102c.
SGDS000000306. EXO84.

Phylogenomic databases

eggNOGNOG321873.
HOGENOMHOG000000727.
OMAVRANIND.
OrthoDBEOG7CG77P.

Enzyme and pathway databases

BioCycYEAST:G3O-29064-MONOMER.

Gene expression databases

GenevestigatorP38261.

Family and domain databases

InterProIPR016159. Cullin_repeat-like_dom.
[Graphical view]
SUPFAMSSF74788. SSF74788. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP38261.
NextBio971228.
PROP38261.

Entry information

Entry nameEXO84_YEAST
AccessionPrimary (citable) accession number: P38261
Secondary accession number(s): D6VQA1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 11, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references