Hsp70 nucleotide exchange factor FES1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38260 (FES1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Hsp70 nucleotide exchange factor FES1

Alternative name(s):
Factor exchange for SSA1 protein 1

Gene names

Name:	FES1
Ordered Locus Names:	YBR101C
ORF Names:	YBR0830

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in protein translation, propagation of [PSI+] prions, and polyamine tolerance. Functions as a nucleotide exchange factor (NEF), which accelerates the release of ADP, for the cytosolic Hsp70 chaperone SSA1 and the ribosome-associated Hsp70 chaperone SSB1. Required for fully efficient Hsp70-mediated folding of proteins. Ref.5 Ref.6 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16
Subunit structure	Interacts with the Hsp70 chaperones SSA1 and SSB1. Ref.6 Ref.16
Subcellular location	Cytoplasm Ref.6 Ref.7.
Miscellaneous	Present with 13100 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the FES1 family. Contains 6 ARM repeats.

Ontologies

Keywords
Biological process	Translation regulation
Cellular component	Cytoplasm
Domain	Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Inferred from mutant phenotype Ref.6. Source: SGD regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosolic ribosome Inferred from physical interaction Ref.6. Source: SGD
Molecular_function	adenyl-nucleotide exchange factor activity Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
ARO8	P53090	1	EBI-21563,EBI-2042933
CYS4	P32582	1	EBI-21563,EBI-4167
GLN1	P32288	1	EBI-21563,EBI-7665
VMA4	P22203	1	EBI-21563,EBI-20268
YCG1	Q06680	1	EBI-21563,EBI-4799

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 290

290

Hsp70 nucleotide exchange factor FES1

PRO_0000202485

Regions

Repeat

13 – 57

ARM 1

Repeat

76 – 116

ARM 2

Repeat

120 – 161

ARM 3

Repeat

164 – 205

ARM 4

Repeat

211 – 251

ARM 5

Repeat

253 – 290

ARM 6

Amino acid modifications

Modified residue

Phosphoserine Ref.17

Sequences

Sequence

Length

Mass (Da)

Tools

P38260 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 4A50D069625BA500
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FASTA

290

32,604

        10         20         30         40         50         60 
MEKLLQWSIA NSQGDKEAMA RAGQPDPKLL QQLFGGGGPD DPTLMKESMA VIMNPEVDLE 

        70         80         90        100        110        120 
TKLVAFDNFE MLIENLDNAN NIENLKLWEP LLDVLVQTKD EELRAAALSI IGTAVQNNLD 

       130        140        150        160        170        180 
SQNNFMKYDN GLRSLIEIAS DKTKPLDVRT KAFYALSNLI RNHKDISEKF FKLNGLDCIA 

       190        200        210        220        230        240 
PVLSDNTAKP KLKMRAIALL TAYLSSVKID ENIISVLRKD GVIESTIECL SDESNLNIID 

       250        260        270        280        290 
RVLSFLSHLI SSGIKFNEQE LHKLNEGYKH IEPLKDRLNE DDYLAVKYVL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 70 kb region on the right arm of yeast chromosome II." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"HspBP1, a homologue of the yeast Fes1 and Sls1 proteins, is an Hsc70 nucleotide exchange factor." Kabani M., McLellan C., Raynes D.A., Guerriero V., Brodsky J.L. FEBS Lett. 531:339-342(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Nucleotide exchange factor for the yeast Hsp70 molecular chaperone Ssa1p." Kabani M., Beckerich J.-M., Brodsky J.L. Mol. Cell. Biol. 22:4677-4689(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SSA1.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Propagation of Saccharomyces cerevisiae [PSI+] prion is impaired by factors that regulate Hsp70 substrate binding." Jones G., Song Y., Chung S., Masison D.C. Mol. Cell. Biol. 24:3928-3937(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Distinct but overlapping functions of Hsp70, Hsp90, and an Hsp70 nucleotide exchange factor during protein biogenesis in yeast." Ahner A., Whyte F.M., Brodsky J.L. Arch. Biochem. Biophys. 435:32-41(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"Mechanism of polyamine tolerance in yeast: novel regulators and insights." Porat Z., Wender N., Erez O., Kahana C. Cell. Mol. Life Sci. 62:3106-3116(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"AGP2 encodes the major permease for high affinity polyamine import in Saccharomyces cerevisiae." Aouida M., Leduc A., Poulin R., Ramotar D. J. Biol. Chem. 280:24267-24276(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[13]	"Regulation of Hsp70 function by HspBP1: structural analysis reveals an alternate mechanism for Hsp70 nucleotide exchange." Shomura Y., Dragovic Z., Chang H.-C., Tzvetkov N., Young J.C., Brodsky J.L., Guerriero V. Jr., Hartl F.U., Bracher A. Mol. Cell 17:367-379(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[14]	"Chaperone network in the yeast cytosol: Hsp110 is revealed as an Hsp70 nucleotide exchange factor." Raviol H., Sadlish H., Rodriguez F., Mayer M.P., Bukau B. EMBO J. 25:2510-2518(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[15]	"Molecular chaperones of the Hsp110 family act as nucleotide exchange factors of Hsp70s." Dragovic Z., Broadley S.A., Shomura Y., Bracher A., Hartl F.U. EMBO J. 25:2519-2528(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[16]	"Fes1p acts as a nucleotide exchange factor for the ribosome-associated molecular chaperone Ssb1p." Dragovic Z., Shomura Y., Tzvetkov N., Hartl F.U., Bracher A. Biol. Chem. 387:1593-1600(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SSB1.
[17]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78993 Genomic DNA. Translation: CAA55604.1. Z35970 Genomic DNA. Translation: CAA85056.1. AY693000 Genomic DNA. Translation: AAT93019.1. BK006936 Genomic DNA. Translation: DAA07220.1.
PIR	S48266.
RefSeq	NP_009659.3. NM_001178449.3.
3D structure databases
ProteinModelPortal	P38260.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4918N.
IntAct	P38260. 14 interactions.
MINT	MINT-478258.
STRING	4932.YBR101C.
Proteomic databases
PaxDb	P38260.
PeptideAtlas	P38260.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR101C; YBR101C; YBR101C.
GeneID	852397.
KEGG	sce:YBR101C. sce:YBR104W.
Organism-specific databases
CYGD	YBR101c.
SGD	S000000305. FES1.
Phylogenomic databases
eggNOG	NOG310365.
GeneTree	ENSGT00560000078421.
HOGENOM	HOG000000726.
KO	K15109.
OMA	FEMLIEN.
OrthoDB	EOG470XT2.
Gene expression databases
Genevestigator	P38260.
GermOnline	YBR101C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.25.10.10. 1 hit.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR013918. Nucleotide_exch_fac_Fes1. [Graphical view]
Pfam	PF08609. Fes1. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50176. ARM_REPEAT. False negative. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL6102.
NextBio	971225.

Entry information

Entry name

FES1_YEAST

Accession

Primary (citable) accession number: P38260
Secondary accession number(s): D6VQA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents