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P38260

- FES1_YEAST

UniProt

P38260 - FES1_YEAST

Protein

Hsp70 nucleotide exchange factor FES1

Gene

FES1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Involved in protein translation, propagation of [PSI+] prions, and polyamine tolerance. Functions as a nucleotide exchange factor (NEF), which accelerates the release of ADP, for the cytosolic Hsp70 chaperone SSA1 and the ribosome-associated Hsp70 chaperone SSB1. Required for fully efficient Hsp70-mediated folding of proteins.10 Publications

    GO - Molecular functioni

    1. adenyl-nucleotide exchange factor activity Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process Source: SGD
    2. cytoplasmic translation Source: SGD
    3. regulation of catalytic activity Source: GOC
    4. regulation of translation Source: UniProtKB-KW

    Keywords - Biological processi

    Translation regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29063-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hsp70 nucleotide exchange factor FES1
    Alternative name(s):
    Factor exchange for SSA1 protein 1
    Gene namesi
    Name:FES1
    Ordered Locus Names:YBR101C
    ORF Names:YBR0830
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR101c.
    SGDiS000000305. FES1.

    Subcellular locationi

    Cytoplasm 2 Publications

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. cytosolic ribosome Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 290290Hsp70 nucleotide exchange factor FES1PRO_0000202485Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei12 – 121Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38260.
    PaxDbiP38260.
    PeptideAtlasiP38260.

    Expressioni

    Gene expression databases

    GenevestigatoriP38260.

    Interactioni

    Subunit structurei

    Interacts with the Hsp70 chaperones SSA1 and SSB1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SSA1P105913EBI-21563,EBI-8591

    Protein-protein interaction databases

    BioGridi32805. 19 interactions.
    DIPiDIP-4918N.
    IntActiP38260. 14 interactions.
    MINTiMINT-478258.
    STRINGi4932.YBR101C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38260.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati13 – 5745ARM 1Add
    BLAST
    Repeati76 – 11641ARM 2Add
    BLAST
    Repeati120 – 16142ARM 3Add
    BLAST
    Repeati164 – 20542ARM 4Add
    BLAST
    Repeati211 – 25141ARM 5Add
    BLAST
    Repeati253 – 29038ARM 6Add
    BLAST

    Sequence similaritiesi

    Belongs to the FES1 family.Curated
    Contains 6 ARM repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG310365.
    HOGENOMiHOG000000726.
    OMAiFEMLIEN.
    OrthoDBiEOG776T0T.

    Family and domain databases

    Gene3Di1.25.10.10. 1 hit.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR013918. Nucleotide_exch_fac_Fes1.
    [Graphical view]
    PfamiPF08609. Fes1. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P38260-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKLLQWSIA NSQGDKEAMA RAGQPDPKLL QQLFGGGGPD DPTLMKESMA    50
    VIMNPEVDLE TKLVAFDNFE MLIENLDNAN NIENLKLWEP LLDVLVQTKD 100
    EELRAAALSI IGTAVQNNLD SQNNFMKYDN GLRSLIEIAS DKTKPLDVRT 150
    KAFYALSNLI RNHKDISEKF FKLNGLDCIA PVLSDNTAKP KLKMRAIALL 200
    TAYLSSVKID ENIISVLRKD GVIESTIECL SDESNLNIID RVLSFLSHLI 250
    SSGIKFNEQE LHKLNEGYKH IEPLKDRLNE DDYLAVKYVL 290
    Length:290
    Mass (Da):32,604
    Last modified:October 1, 1994 - v1
    Checksum:i4A50D069625BA500
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78993 Genomic DNA. Translation: CAA55604.1.
    Z35970 Genomic DNA. Translation: CAA85056.1.
    AY693000 Genomic DNA. Translation: AAT93019.1.
    BK006936 Genomic DNA. Translation: DAA07220.1.
    PIRiS48266.
    RefSeqiNP_009659.3. NM_001178449.3.

    Genome annotation databases

    EnsemblFungiiYBR101C; YBR101C; YBR101C.
    GeneIDi852397.
    KEGGisce:YBR101C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78993 Genomic DNA. Translation: CAA55604.1 .
    Z35970 Genomic DNA. Translation: CAA85056.1 .
    AY693000 Genomic DNA. Translation: AAT93019.1 .
    BK006936 Genomic DNA. Translation: DAA07220.1 .
    PIRi S48266.
    RefSeqi NP_009659.3. NM_001178449.3.

    3D structure databases

    ProteinModelPortali P38260.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32805. 19 interactions.
    DIPi DIP-4918N.
    IntActi P38260. 14 interactions.
    MINTi MINT-478258.
    STRINGi 4932.YBR101C.

    Chemistry

    ChEMBLi CHEMBL6102.

    Proteomic databases

    MaxQBi P38260.
    PaxDbi P38260.
    PeptideAtlasi P38260.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR101C ; YBR101C ; YBR101C .
    GeneIDi 852397.
    KEGGi sce:YBR101C.

    Organism-specific databases

    CYGDi YBR101c.
    SGDi S000000305. FES1.

    Phylogenomic databases

    eggNOGi NOG310365.
    HOGENOMi HOG000000726.
    OMAi FEMLIEN.
    OrthoDBi EOG776T0T.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29063-MONOMER.

    Miscellaneous databases

    NextBioi 971225.

    Gene expression databases

    Genevestigatori P38260.

    Family and domain databases

    Gene3Di 1.25.10.10. 1 hit.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR013918. Nucleotide_exch_fac_Fes1.
    [Graphical view ]
    Pfami PF08609. Fes1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
      Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
      Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "HspBP1, a homologue of the yeast Fes1 and Sls1 proteins, is an Hsc70 nucleotide exchange factor."
      Kabani M., McLellan C., Raynes D.A., Guerriero V., Brodsky J.L.
      FEBS Lett. 531:339-342(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Nucleotide exchange factor for the yeast Hsp70 molecular chaperone Ssa1p."
      Kabani M., Beckerich J.-M., Brodsky J.L.
      Mol. Cell. Biol. 22:4677-4689(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SSA1.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Propagation of Saccharomyces cerevisiae [PSI+] prion is impaired by factors that regulate Hsp70 substrate binding."
      Jones G., Song Y., Chung S., Masison D.C.
      Mol. Cell. Biol. 24:3928-3937(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Distinct but overlapping functions of Hsp70, Hsp90, and an Hsp70 nucleotide exchange factor during protein biogenesis in yeast."
      Ahner A., Whyte F.M., Brodsky J.L.
      Arch. Biochem. Biophys. 435:32-41(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Mechanism of polyamine tolerance in yeast: novel regulators and insights."
      Porat Z., Wender N., Erez O., Kahana C.
      Cell. Mol. Life Sci. 62:3106-3116(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "AGP2 encodes the major permease for high affinity polyamine import in Saccharomyces cerevisiae."
      Aouida M., Leduc A., Poulin R., Ramotar D.
      J. Biol. Chem. 280:24267-24276(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. "Regulation of Hsp70 function by HspBP1: structural analysis reveals an alternate mechanism for Hsp70 nucleotide exchange."
      Shomura Y., Dragovic Z., Chang H.-C., Tzvetkov N., Young J.C., Brodsky J.L., Guerriero V. Jr., Hartl F.U., Bracher A.
      Mol. Cell 17:367-379(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "Chaperone network in the yeast cytosol: Hsp110 is revealed as an Hsp70 nucleotide exchange factor."
      Raviol H., Sadlish H., Rodriguez F., Mayer M.P., Bukau B.
      EMBO J. 25:2510-2518(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    15. "Molecular chaperones of the Hsp110 family act as nucleotide exchange factors of Hsp70s."
      Dragovic Z., Broadley S.A., Shomura Y., Bracher A., Hartl F.U.
      EMBO J. 25:2519-2528(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    16. "Fes1p acts as a nucleotide exchange factor for the ribosome-associated molecular chaperone Ssb1p."
      Dragovic Z., Shomura Y., Tzvetkov N., Hartl F.U., Bracher A.
      Biol. Chem. 387:1593-1600(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SSB1.
    17. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiFES1_YEAST
    AccessioniPrimary (citable) accession number: P38260
    Secondary accession number(s): D6VQA0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 13100 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3