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P38260

- FES1_YEAST

UniProt

P38260 - FES1_YEAST

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Protein

Hsp70 nucleotide exchange factor FES1

Gene
FES1, YBR101C, YBR0830
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in protein translation, propagation of [PSI+] prions, and polyamine tolerance. Functions as a nucleotide exchange factor (NEF), which accelerates the release of ADP, for the cytosolic Hsp70 chaperone SSA1 and the ribosome-associated Hsp70 chaperone SSB1. Required for fully efficient Hsp70-mediated folding of proteins.10 Publications

GO - Molecular functioni

  1. adenyl-nucleotide exchange factor activity Source: SGD
  2. protein binding Source: IntAct

GO - Biological processi

  1. cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  2. cytoplasmic translation Source: SGD
  3. regulation of catalytic activity Source: GOC
  4. regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Translation regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29063-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hsp70 nucleotide exchange factor FES1
Alternative name(s):
Factor exchange for SSA1 protein 1
Gene namesi
Name:FES1
Ordered Locus Names:YBR101C
ORF Names:YBR0830
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR101c.
SGDiS000000305. FES1.

Subcellular locationi

Cytoplasm 2 Publications

GO - Cellular componenti

  1. cytosol Source: SGD
  2. cytosolic ribosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 290290Hsp70 nucleotide exchange factor FES1PRO_0000202485Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38260.
PaxDbiP38260.
PeptideAtlasiP38260.

Expressioni

Gene expression databases

GenevestigatoriP38260.

Interactioni

Subunit structurei

Interacts with the Hsp70 chaperones SSA1 and SSB1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SSA1P105913EBI-21563,EBI-8591

Protein-protein interaction databases

BioGridi32805. 19 interactions.
DIPiDIP-4918N.
IntActiP38260. 14 interactions.
MINTiMINT-478258.
STRINGi4932.YBR101C.

Structurei

3D structure databases

ProteinModelPortaliP38260.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati13 – 5745ARM 1Add
BLAST
Repeati76 – 11641ARM 2Add
BLAST
Repeati120 – 16142ARM 3Add
BLAST
Repeati164 – 20542ARM 4Add
BLAST
Repeati211 – 25141ARM 5Add
BLAST
Repeati253 – 29038ARM 6Add
BLAST

Sequence similaritiesi

Belongs to the FES1 family.
Contains 6 ARM repeats.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG310365.
HOGENOMiHOG000000726.
OMAiFEMLIEN.
OrthoDBiEOG776T0T.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013918. Nucleotide_exch_fac_Fes1.
[Graphical view]
PfamiPF08609. Fes1. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

P38260-1 [UniParc]FASTAAdd to Basket

« Hide

MEKLLQWSIA NSQGDKEAMA RAGQPDPKLL QQLFGGGGPD DPTLMKESMA    50
VIMNPEVDLE TKLVAFDNFE MLIENLDNAN NIENLKLWEP LLDVLVQTKD 100
EELRAAALSI IGTAVQNNLD SQNNFMKYDN GLRSLIEIAS DKTKPLDVRT 150
KAFYALSNLI RNHKDISEKF FKLNGLDCIA PVLSDNTAKP KLKMRAIALL 200
TAYLSSVKID ENIISVLRKD GVIESTIECL SDESNLNIID RVLSFLSHLI 250
SSGIKFNEQE LHKLNEGYKH IEPLKDRLNE DDYLAVKYVL 290
Length:290
Mass (Da):32,604
Last modified:October 1, 1994 - v1
Checksum:i4A50D069625BA500
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78993 Genomic DNA. Translation: CAA55604.1.
Z35970 Genomic DNA. Translation: CAA85056.1.
AY693000 Genomic DNA. Translation: AAT93019.1.
BK006936 Genomic DNA. Translation: DAA07220.1.
PIRiS48266.
RefSeqiNP_009659.3. NM_001178449.3.

Genome annotation databases

EnsemblFungiiYBR101C; YBR101C; YBR101C.
GeneIDi852397.
KEGGisce:YBR101C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78993 Genomic DNA. Translation: CAA55604.1 .
Z35970 Genomic DNA. Translation: CAA85056.1 .
AY693000 Genomic DNA. Translation: AAT93019.1 .
BK006936 Genomic DNA. Translation: DAA07220.1 .
PIRi S48266.
RefSeqi NP_009659.3. NM_001178449.3.

3D structure databases

ProteinModelPortali P38260.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32805. 19 interactions.
DIPi DIP-4918N.
IntActi P38260. 14 interactions.
MINTi MINT-478258.
STRINGi 4932.YBR101C.

Chemistry

ChEMBLi CHEMBL6102.

Proteomic databases

MaxQBi P38260.
PaxDbi P38260.
PeptideAtlasi P38260.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR101C ; YBR101C ; YBR101C .
GeneIDi 852397.
KEGGi sce:YBR101C.

Organism-specific databases

CYGDi YBR101c.
SGDi S000000305. FES1.

Phylogenomic databases

eggNOGi NOG310365.
HOGENOMi HOG000000726.
OMAi FEMLIEN.
OrthoDBi EOG776T0T.

Enzyme and pathway databases

BioCyci YEAST:G3O-29063-MONOMER.

Miscellaneous databases

NextBioi 971225.

Gene expression databases

Genevestigatori P38260.

Family and domain databases

Gene3Di 1.25.10.10. 1 hit.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013918. Nucleotide_exch_fac_Fes1.
[Graphical view ]
Pfami PF08609. Fes1. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
    Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
    Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "HspBP1, a homologue of the yeast Fes1 and Sls1 proteins, is an Hsc70 nucleotide exchange factor."
    Kabani M., McLellan C., Raynes D.A., Guerriero V., Brodsky J.L.
    FEBS Lett. 531:339-342(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Nucleotide exchange factor for the yeast Hsp70 molecular chaperone Ssa1p."
    Kabani M., Beckerich J.-M., Brodsky J.L.
    Mol. Cell. Biol. 22:4677-4689(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SSA1.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Propagation of Saccharomyces cerevisiae [PSI+] prion is impaired by factors that regulate Hsp70 substrate binding."
    Jones G., Song Y., Chung S., Masison D.C.
    Mol. Cell. Biol. 24:3928-3937(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Distinct but overlapping functions of Hsp70, Hsp90, and an Hsp70 nucleotide exchange factor during protein biogenesis in yeast."
    Ahner A., Whyte F.M., Brodsky J.L.
    Arch. Biochem. Biophys. 435:32-41(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Mechanism of polyamine tolerance in yeast: novel regulators and insights."
    Porat Z., Wender N., Erez O., Kahana C.
    Cell. Mol. Life Sci. 62:3106-3116(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "AGP2 encodes the major permease for high affinity polyamine import in Saccharomyces cerevisiae."
    Aouida M., Leduc A., Poulin R., Ramotar D.
    J. Biol. Chem. 280:24267-24276(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Regulation of Hsp70 function by HspBP1: structural analysis reveals an alternate mechanism for Hsp70 nucleotide exchange."
    Shomura Y., Dragovic Z., Chang H.-C., Tzvetkov N., Young J.C., Brodsky J.L., Guerriero V. Jr., Hartl F.U., Bracher A.
    Mol. Cell 17:367-379(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Chaperone network in the yeast cytosol: Hsp110 is revealed as an Hsp70 nucleotide exchange factor."
    Raviol H., Sadlish H., Rodriguez F., Mayer M.P., Bukau B.
    EMBO J. 25:2510-2518(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. "Molecular chaperones of the Hsp110 family act as nucleotide exchange factors of Hsp70s."
    Dragovic Z., Broadley S.A., Shomura Y., Bracher A., Hartl F.U.
    EMBO J. 25:2519-2528(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  16. "Fes1p acts as a nucleotide exchange factor for the ribosome-associated molecular chaperone Ssb1p."
    Dragovic Z., Shomura Y., Tzvetkov N., Hartl F.U., Bracher A.
    Biol. Chem. 387:1593-1600(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SSB1.
  17. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFES1_YEAST
AccessioniPrimary (citable) accession number: P38260
Secondary accession number(s): D6VQA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 11, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 13100 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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