P38255 (RXT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional regulatory protein RXT2

Gene names

Name:	RXT2
Synonyms:	RAF60
Ordered Locus Names:	YBR095C
ORF Names:	YBR0822

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the RPD3C(L) histone deacetylase complex (HDAC) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Ref.10
Subunit structure	Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, UME1 and UME6.
Subcellular location	Nucleus Ref.7.
Miscellaneous	Present with 1630 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the RXT2 family.
Sequence caution	The sequence AAT92704.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA49507.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA55600.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA85048.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Chromatin regulator Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	conjugation with cellular fusion Inferred from mutant phenotype. Source: SGD histone deacetylation Inferred from mutant phenotype Ref.10. Source: GOC invasive growth in response to glucose limitation Inferred from mutant phenotype. Source: SGD negative regulation of chromatin silencing at rDNA Inferred from mutant phenotype Ref.11. Source: SGD negative regulation of chromatin silencing at silent mating-type cassette Inferred from mutant phenotype Ref.11. Source: SGD negative regulation of chromatin silencing at telomere Inferred from mutant phenotype Ref.11Ref.9. Source: SGD negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype Ref.10. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Rpd3L complex Inferred from direct assay Ref.11Ref.9. Source: SGD Rpd3L-Expanded complex Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RPD3	P32561	6	EBI-21537,EBI-15864

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Transcriptional regulatory protein RXT2		PRO_0000202482
Amino acid modifications
	Modified residue	249	1	Phosphoserine Ref.12

Sequences

	Sequence		Length	Mass (Da)
	P38255 [UniParc]. Last modified June 13, 2006. Version 2. Checksum: A78CCBC603E2316C	FASTA	430	48,629
	10 20 30 40 50 60 MTIRSSMKNN AELESKSVLA NESNIISTFT RRIIKEKSGN YQVLKRSLDG KLIYPEATGI 70 80 90 100 110 120 SSNRGNKLLQ RSEVVTRRDL NNSKPMIEQT VFYNGSEHRL LQTNIVTDSR RKRIKFTPDI 130 140 150 160 170 180 NVEPVLVGDE NDIDGSEKED ENITDEYYGE EDDDDLSKLV NVKEILTPIL SLGDIINHKT 190 200 210 220 230 240 ISRTFSSPIL KNLALQIILM IEKEQMSVVR YSQFLEVFLG DHPEPIYESN LNLPSYNHNL 250 260 270 280 290 300 TLPEDRGASD EDDINNKNNI NEVNSNSLST EAGHINNGME EFGEEDPFFA LPRLEQSNAL 310 320 330 340 350 360 LSLLPSSSGS ASISTLTAAE QQQLNEEIES ARQLSQIALQ RNKEFIRNLQ KIRKSVIKAN 370 380 390 400 410 420 RIRGRILNWS REYLGISDDD ITIPVALRVV KRGLISATTN KTTNFEEEIE NTMEDGVVDD 430 NEPDEEANRA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Dekker P.J.T., Hoekert W., van Oosterum K., Grivell L.A. Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26109 / X2180.
[2]	"Analysis of a 70 kb region on the right arm of yeast chromosome II." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 10:1363-1381(1994) [PubMed: 7900426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Stable incorporation of sequence specific repressors Ash1 and Ume6 into the Rpd3L complex." Carrozza M.J., Florens L., Swanson S.K., Shia W.-J., Anderson S., Yates J., Washburn M.P., Workman J.L. Biochim. Biophys. Acta 1731:77-87(2005) [PubMed: 16314178] [Abstract] Cited for: IDENTIFICATION IN THE RPD3 COMPLEX, MASS SPECTROMETRY.
[10]	"Raf60, a novel component of the Rpd3 histone deacetylase complex required for Rpd3 activity in Saccharomyces cerevisiae." Colina A.R., Young D. J. Biol. Chem. 280:42552-42556(2005) [PubMed: 16275642] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE RPD3C(L) COMPLEX, MASS SPECTROMETRY.
[11]	"Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex." Keogh M.-C., Kurdistani S.K., Morris S.A., Ahn S.H., Podolny V., Collins S.R., Schuldiner M., Chin K., Punna T., Thompson N.J., Boone C., Emili A., Weissman J.S., Hughes T.R., Strahl B.D., Grunstein M., Greenblatt J.F., Buratowski S., Krogan N.J. Cell 123:593-605(2005) [PubMed: 16286008] [Abstract] Cited for: IDENTIFICATION IN THE RPD3C(L) COMPLEX, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-249, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69881 Genomic DNA. Translation: CAA49507.1. Different initiation. X78993 Genomic DNA. Translation: CAA55600.1. Different initiation. Z35964 Genomic DNA. Translation: CAA85048.1. Different initiation. AY692685 Genomic DNA. Translation: AAT92704.1. Different initiation. BK006936 Genomic DNA. Translation: DAA07216.1.
PIR	S31313.
RefSeq	NP_009653.2. NM_001178443.1.
3D structure databases
ProteinModelPortal	P38255.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6755N.
IntAct	P38255. 13 interactions.
MINT	MINT-666070.
STRING	P38255.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR095C; YBR095C; YBR095C.
GeneID	852392.
KEGG	sce:YBR095C.
NMPDR	fig|4932.3.peg.352.
Organism-specific databases
CYGD	YBR095c.
SGD	S000000299. RXT2.
Phylogenomic databases
eggNOG	fuNOG08441.
HOGENOM	HBG204154.
OMA	YNGSEHR.
OrthoDB	EOG444PVX.
Gene expression databases
ArrayExpress	P38255.
Genevestigator	P38255.
GermOnline	YBR095C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013904. RXT2_N. [Graphical view]
Pfam	PF08595. RXT2_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971213.

Entry information

Entry name

RXT2_YEAST

Accession

Primary (citable) accession number: P38255
Secondary accession number(s): D6VQ96

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	June 13, 2006
Last modified:	December 14, 2011
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program