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Protein

Probable tubulin--tyrosine ligase PBY1

Gene

PBY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probable P-body-associated tubulin--tyrosine ligase.By similarity1 Publication

Catalytic activityi

ATP + detyrosinated alpha-tubulin + L-tyrosine = alpha-tubulin + ADP + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

Magnesium, Potassium

Enzyme and pathway databases

BioCyciYEAST:G3O-29058-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tubulin--tyrosine ligase PBY1 (EC:6.3.2.25)
Alternative name(s):
P-body-associated protein 1
Gene namesi
Name:PBY1
Ordered Locus Names:YBR094W
ORF Names:YBR0821
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR094W.
SGDiS000000298. PBY1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic mRNA processing body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 753753Probable tubulin--tyrosine ligase PBY1PRO_0000212448Add
BLAST

Proteomic databases

MaxQBiP38254.

PTM databases

iPTMnetiP38254.

Interactioni

Protein-protein interaction databases

BioGridi32800. 13 interactions.
DIPiDIP-1585N.
IntActiP38254. 5 interactions.
MINTiMINT-409689.

Structurei

3D structure databases

ProteinModelPortaliP38254.
SMRiP38254. Positions 108-215, 476-727.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini343 – 734392TTLPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the tubulin--tyrosine ligase family.Curated
Contains 1 TTL domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP38254.
OMAiKEWWILK.
OrthoDBiEOG092C2FY4.

Family and domain databases

Gene3Di3.40.1210.10. 2 hits.
InterProiIPR002828. SurE-like_Pase/nucleotidase.
IPR027746. TTL.
IPR004344. TTL/TTLL_fam.
[Graphical view]
PANTHERiPTHR12241:SF1. PTHR12241:SF1. 2 hits.
PfamiPF01975. SurE. 1 hit.
PF03133. TTL. 1 hit.
[Graphical view]
SUPFAMiSSF64167. SSF64167. 2 hits.
TIGRFAMsiTIGR00087. surE. 1 hit.
PROSITEiPS51221. TTL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38254-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLITNDDG PLSDQFSPYI RPFIQHIKRN YPEWKITVCV PHVQKSWVGK
60 70 80 90 100
AHLAGKNLTA QFIYSKVDAE DNTFWGPFIQ PQIRSENSKL PYVLNAEIPK
110 120 130 140 150
DTIEWILIDG TPASCANIGL HLLSNEPFDL VLSGPNVGRN TSAAYITSSG
160 170 180 190 200
TVGGAMESVI TGNTKAIAIS WAYFNGLKNV SPLLMEKASK RSLDVIKHLV
210 220 230 240 250
KNWDPKTDLY SINIPLVESL SDDTKVYYAP IWENRWIPIF NGPHINLENS
260 270 280 290 300
FAEIEDGNES SSISFNWAPK FGAHKDSIHY MDEYKDRTVL TDAEVIESEM
310 320 330 340 350
ISVTPMKATF KGVNHLLGEL KLTEEENNLS KTNNLIVVSI DPMEYIYKPL
360 370 380 390 400
THALKKYLPQ VEIVSNLPEF DNGGCEKEMK VFHYGDYEQL DMDKLMELPN
410 420 430 440 450
NYFTNSYIYR KALIRKHFLS HTIQTYTAKN PESILKKAYL ESFTIDLDYA
460 470 480 490 500
EFLDDALDEN WELRQELENE SQDKWWIVKP SMSDKGQGIR VFKTIEDLQA
510 520 530 540 550
IFDSFDDEDS EAEESGNDDD ADDVNGEFMD NNKVNISQLR HFIIQEYLTN
560 570 580 590 600
PLLLASMDNR KFHIRCYVVC RGDLQVFVYD RMLALFAAKP FVPLDPYAYS
610 620 630 640 650
VTDLKDLECH LTNTCLQSKK KDKDSSVLEF DSIEEIPNER KSNIKEQIHS
660 670 680 690 700
ITNDVFLAAV NVNRLNFQPL PNAFETYGVD FLIDSNYEVK LLEINAFPDF
710 720 730 740 750
KQTGKDLKNL IDELFDDTVK YCVTPIFNEN RNKTDDETDP NFVKVIDYTS

NGW
Length:753
Mass (Da):86,353
Last modified:July 27, 2011 - v2
Checksum:i925C4E4CAB6679E9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti352 – 3554HALK → TPE in CAA49508 (Ref. 4) Curated
Sequence conflicti450 – 4501A → R in CAA55599 (PubMed:7900426).Curated
Sequence conflicti450 – 4501A → R in CAA85047 (PubMed:7813418).Curated
Sequence conflicti563 – 5631H → R in CAA49508 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78993 Genomic DNA. Translation: CAA55599.1.
Z35963 Genomic DNA. Translation: CAA85047.1.
X69881 Genomic DNA. Translation: CAA49508.1.
BK006936 Genomic DNA. Translation: DAA07215.2.
PIRiS48261.
RefSeqiNP_009652.2. NM_001178442.2.

Genome annotation databases

EnsemblFungiiYBR094W; YBR094W; YBR094W.
GeneIDi852391.
KEGGisce:YBR094W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78993 Genomic DNA. Translation: CAA55599.1.
Z35963 Genomic DNA. Translation: CAA85047.1.
X69881 Genomic DNA. Translation: CAA49508.1.
BK006936 Genomic DNA. Translation: DAA07215.2.
PIRiS48261.
RefSeqiNP_009652.2. NM_001178442.2.

3D structure databases

ProteinModelPortaliP38254.
SMRiP38254. Positions 108-215, 476-727.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32800. 13 interactions.
DIPiDIP-1585N.
IntActiP38254. 5 interactions.
MINTiMINT-409689.

PTM databases

iPTMnetiP38254.

Proteomic databases

MaxQBiP38254.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR094W; YBR094W; YBR094W.
GeneIDi852391.
KEGGisce:YBR094W.

Organism-specific databases

EuPathDBiFungiDB:YBR094W.
SGDiS000000298. PBY1.

Phylogenomic databases

InParanoidiP38254.
OMAiKEWWILK.
OrthoDBiEOG092C2FY4.

Enzyme and pathway databases

BioCyciYEAST:G3O-29058-MONOMER.

Miscellaneous databases

PROiP38254.

Family and domain databases

Gene3Di3.40.1210.10. 2 hits.
InterProiIPR002828. SurE-like_Pase/nucleotidase.
IPR027746. TTL.
IPR004344. TTL/TTLL_fam.
[Graphical view]
PANTHERiPTHR12241:SF1. PTHR12241:SF1. 2 hits.
PfamiPF01975. SurE. 1 hit.
PF03133. TTL. 1 hit.
[Graphical view]
SUPFAMiSSF64167. SSF64167. 2 hits.
TIGRFAMsiTIGR00087. surE. 1 hit.
PROSITEiPS51221. TTL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTTL_YEAST
AccessioniPrimary (citable) accession number: P38254
Secondary accession number(s): D6VQ95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1770 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.