P38254 (TTL_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tubulin--tyrosine ligase PBY1
EC=6.3.2.25

Alternative name(s):
P-body-associated protein 1

Gene names

Name:	PBY1
Ordered Locus Names:	YBR094W
ORF Names:	YBR0821

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	753 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable P-body-associated tubuline--tyrosine ligase By similarity. Ref.7
Catalytic activity	ATP + detyrosinated alpha-tubulin + L-tyrosine = alpha-tubulin + ADP + phosphate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Subcellular location	Cytoplasm. Cytoplasm › P-body Ref.5 Ref.7.
Miscellaneous	Present with 1770 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the tubulin--tyrosine ligase family. Contains 1 TTL domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Potassium
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasmic mRNA processing body Inferred from direct assay Ref.7. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro hydrolase activity Inferred from electronic annotation. Source: InterPro tubulin-tyrosine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DCP1	Q12517	3	EBI-21533,EBI-38519

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 753	753	Probable tubulin--tyrosine ligase PBY1		PRO_0000212448
Regions
	Domain	343 – 734	392	TTL
Amino acid modifications
	Modified residue	600	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	352 – 355	4	HALK → TPE in CAA49508. Ref.4
	Sequence conflict	450	1	A → R in CAA55599. Ref.1
	Sequence conflict	450	1	A → R in CAA85047. Ref.2
	Sequence conflict	563	1	H → R in CAA49508. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P38254 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 925C4E4CAB6679E9	FASTA	753	86,353
	10 20 30 40 50 60 MRVLITNDDG PLSDQFSPYI RPFIQHIKRN YPEWKITVCV PHVQKSWVGK AHLAGKNLTA 70 80 90 100 110 120 QFIYSKVDAE DNTFWGPFIQ PQIRSENSKL PYVLNAEIPK DTIEWILIDG TPASCANIGL 130 140 150 160 170 180 HLLSNEPFDL VLSGPNVGRN TSAAYITSSG TVGGAMESVI TGNTKAIAIS WAYFNGLKNV 190 200 210 220 230 240 SPLLMEKASK RSLDVIKHLV KNWDPKTDLY SINIPLVESL SDDTKVYYAP IWENRWIPIF 250 260 270 280 290 300 NGPHINLENS FAEIEDGNES SSISFNWAPK FGAHKDSIHY MDEYKDRTVL TDAEVIESEM 310 320 330 340 350 360 ISVTPMKATF KGVNHLLGEL KLTEEENNLS KTNNLIVVSI DPMEYIYKPL THALKKYLPQ 370 380 390 400 410 420 VEIVSNLPEF DNGGCEKEMK VFHYGDYEQL DMDKLMELPN NYFTNSYIYR KALIRKHFLS 430 440 450 460 470 480 HTIQTYTAKN PESILKKAYL ESFTIDLDYA EFLDDALDEN WELRQELENE SQDKWWIVKP 490 500 510 520 530 540 SMSDKGQGIR VFKTIEDLQA IFDSFDDEDS EAEESGNDDD ADDVNGEFMD NNKVNISQLR 550 560 570 580 590 600 HFIIQEYLTN PLLLASMDNR KFHIRCYVVC RGDLQVFVYD RMLALFAAKP FVPLDPYAYS 610 620 630 640 650 660 VTDLKDLECH LTNTCLQSKK KDKDSSVLEF DSIEEIPNER KSNIKEQIHS ITNDVFLAAV 670 680 690 700 710 720 NVNRLNFQPL PNAFETYGVD FLIDSNYEVK LLEINAFPDF KQTGKDLKNL IDELFDDTVK 730 740 750 YCVTPIFNEN RNKTDDETDP NFVKVIDYTS NGW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 70 kb region on the right arm of yeast chromosome II." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 10:1363-1381(1994) [PubMed: 7900426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 450. Strain: ATCC 204508 / S288c.
[4]	Dekker P.J.T., Hoekert W., van Oosterum K., Grivell L.A. Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 167-753. Strain: ATCC 26109 / X2180.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Microtubule disruption stimulates P-body formation." Sweet T.J., Boyer B., Hu W., Baker K.E., Coller J. RNA 13:493-502(2007) [PubMed: 17307817] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-600, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78993 Genomic DNA. Translation: CAA55599.1. Z35963 Genomic DNA. Translation: CAA85047.1. X69881 Genomic DNA. Translation: CAA49508.1. BK006936 Genomic DNA. Translation: DAA07215.2.
PIR	S48261.
RefSeq	NP_009652.2. NM_001178442.2.
3D structure databases
ProteinModelPortal	P38254.
SMR	P38254. Positions 2-317, 476-505, 672-699.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1585N.
IntAct	P38254. 8 interactions.
MINT	MINT-409689.
STRING	P38254.
Proteomic databases
PeptideAtlas	P38254.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	852391.
KEGG	sce:YBR094W.
NMPDR	fig|4932.3.peg.351.
Organism-specific databases
CYGD	YBR094w.
SGD	S000000298. PBY1.
Phylogenomic databases
eggNOG	fuNOG04394.
GeneTree	EFGT00050000003168.
HOGENOM	HBG324223.
OMA	DFKQTGD.
OrthoDB	EOG4HMNJK.
Gene expression databases
ArrayExpress	P38254.
Genevestigator	P38254.
GermOnline	YBR094W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013816. ATP_grasp_subdomain_2. IPR002828. SurE-like_Pase/nucleotidase. IPR004344. Tub_tyr_ligase. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.1210.10. SurE-like_Pase/nucleotidase. 2 hits.
PANTHER	PTHR12241. Tub_tyr_ligase. 1 hit.
Pfam	PF01975. SurE. 1 hit. PF03133. TTL. 1 hit. [Graphical view]
SUPFAM	SSF64167. SurE-like_Pase/nucleotidase. 1 hit.
TIGRFAMs	TIGR00087. SurE. 1 hit.
PROSITE	PS51221. TTL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971210.

Entry information

Entry name

TTL_YEAST

Accession

Primary (citable) accession number: P38254
Secondary accession number(s): D6VQ95

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	July 27, 2011
Last modified:	December 14, 2011
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program