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Protein

Replication factor C subunit 5

Gene

RFC5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of ATP-dependent clamp loader (RFC and RFC-like) complexes for DNA clamps, such as the POL30/PCNA homotrimer and the checkpoint clamp DDC1:MEC3:RAD17 complex. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Component of the replication factor C (RFC or activator 1) complex which loads POL30/PCNA and acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. Component of the RFC-like RAD24-RFC complex which loads the checkpoint clamp DDC1:MEC3:RAD17 complex and is involved in DNA repair pathways. Component of the RFC-like ELG1-RFC complex which appears to have a role in DNA replication, replication fork re-start, recombination and repair.5 Publications

Miscellaneous

Present with 5040 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei5ATP; via carbonyl oxygen1
Binding sitei17ATP1
Binding sitei231ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi43 – 51ATP9

GO - Molecular functioni

GO - Biological processi

  • DNA repair Source: GO_Central
  • leading strand elongation Source: SGD
  • sister chromatid cohesion Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processCell cycle, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29054-MONOMER
ReactomeiR-SCE-110312 Translesion synthesis by REV1
R-SCE-110314 Recognition of DNA damage by PCNA-containing replication complex
R-SCE-110320 Translesion Synthesis by POLH
R-SCE-174411 Polymerase switching on the C-strand of the telomere
R-SCE-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-SCE-5655862 Translesion synthesis by POLK
R-SCE-5656121 Translesion synthesis by POLI
R-SCE-5656169 Termination of translesion DNA synthesis
R-SCE-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-SCE-5696400 Dual Incision in GG-NER
R-SCE-6782135 Dual incision in TC-NER
R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SCE-69091 Polymerase switching

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C subunit 5
Short name:
Replication factor C5
Alternative name(s):
Activator 1 40 kDa subunit
Gene namesi
Name:RFC5
Ordered Locus Names:YBR087W
ORF Names:YBR0810
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR087W
SGDiS000000291 RFC5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001217651 – 354Replication factor C subunit 5Add BLAST354

Proteomic databases

MaxQBiP38251
PaxDbiP38251
PRIDEiP38251

PTM databases

iPTMnetiP38251

Interactioni

Subunit structurei

Replication factor C (RFC) is a heteropentamer of subunits RFC1, RFC2, RFC3, RFC4 and RFC5 and forms a complex with POL30/PCNA in the presence of ATP. Component of the RAD24-RFC complex which consists of RAD24, RFC2, RFC3, RFC4 and RFC5 and associates with the checkpoint clamp DDC1:MEC3:RAD17 complex. Component of the ELG1-RFC complex which consists of ELG1, RFC2, RFC3, RFC4 and RFC5. Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. RFC5 interacts with ECO1.9 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32793, 257 interactors
DIPiDIP-2531N
IntActiP38251, 15 interactors
MINTiP38251
STRINGi4932.YBR087W

Structurei

Secondary structure

1354
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni5 – 8Combined sources4
Helixi13 – 15Combined sources3
Helixi20 – 27Combined sources8
Turni28 – 31Combined sources4
Beta strandi39 – 42Combined sources4
Helixi49 – 54Combined sources6
Helixi56 – 60Combined sources5
Beta strandi91 – 96Combined sources6
Helixi106 – 116Combined sources11
Turni117 – 120Combined sources4
Beta strandi136 – 141Combined sources6
Helixi148 – 160Combined sources13
Turni162 – 164Combined sources3
Beta strandi165 – 172Combined sources8
Helixi179 – 182Combined sources4
Beta strandi185 – 189Combined sources5
Helixi195 – 209Combined sources15
Helixi217 – 226Combined sources10
Helixi230 – 237Combined sources8
Helixi239 – 242Combined sources4
Turni243 – 246Combined sources4
Helixi258 – 272Combined sources15
Helixi276 – 290Combined sources15
Turni291 – 293Combined sources3
Helixi296 – 305Combined sources10
Turni306 – 309Combined sources4
Helixi315 – 333Combined sources19
Helixi338 – 353Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SXJX-ray2.85E1-354[»]
ProteinModelPortaliP38251
SMRiP38251
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38251

Family & Domainsi

Sequence similaritiesi

Belongs to the activator 1 small subunits family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075006
HOGENOMiHOG000224153
InParanoidiP38251
KOiK10756
OMAiLSHCIPP
OrthoDBiEOG092C317A

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR008921 DNA_pol3_clamp-load_cplx_C
IPR027417 P-loop_NTPase
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF48019 SSF48019, 1 hit
SSF52540 SSF52540, 1 hit

Sequencei

Sequence statusi: Complete.

P38251-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLWVDKYRP KSLNALSHNE ELTNFLKSLS DQPRDLPHLL LYGPNGTGKK
60 70 80 90 100
TRCMALLESI FGPGVYRLKI DVRQFVTASN RKLELNVVSS PYHLEITPSD
110 120 130 140 150
MGNNDRIVIQ ELLKEVAQME QVDFQDSKDG LAHRYKCVII NEANSLTKDA
160 170 180 190 200
QAALRRTMEK YSKNIRLIMV CDSMSPIIAP IKSRCLLIRC PAPSDSEIST
210 220 230 240 250
ILSDVVTNER IQLETKDILK RIAQASNGNL RVSLLMLESM ALNNELALKS
260 270 280 290 300
SSPIIKPDWI IVIHKLTRKI VKERSVNSLI ECRAVLYDLL AHCIPANIIL
310 320 330 340 350
KELTFSLLDV ETLNTTNKSS IIEYSSVFDE RLSLGNKAIF HLEGFIAKVM

CCLD
Length:354
Mass (Da):39,942
Last modified:October 1, 1994 - v1
Checksum:i4CD403AF2AFA2961
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26031 Genomic DNA Translation: AAC49065.1
X78993 Genomic DNA Translation: CAA55595.1
Z35956 Genomic DNA Translation: CAA85036.1
AY693173 Genomic DNA Translation: AAT93192.1
BK006936 Genomic DNA Translation: DAA07208.1
PIRiS48257
RefSeqiNP_009644.3, NM_001178435.3

Genome annotation databases

EnsemblFungiiYBR087W; YBR087W; YBR087W
GeneIDi852383
KEGGisce:YBR087W

Similar proteinsi

Entry informationi

Entry nameiRFC5_YEAST
AccessioniPrimary (citable) accession number: P38251
Secondary accession number(s): D6VQ88
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 23, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

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