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Protein

Increased sodium tolerance protein 2

Gene

IST2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in ion homeostasis together with BTN1 or BTN2.1 Publication

GO - Molecular functioni

  • lipid binding Source: SGD

GO - Biological processi

  • endoplasmic reticulum membrane organization Source: SGD
  • protein localization to plasma membrane Source: SGD
  • regulation of phosphatidylinositol dephosphorylation Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29053-MONOMER.

Protein family/group databases

TCDBi1.A.17.1.19. the calcium-dependent chloride channel (ca-clc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Increased sodium tolerance protein 2
Gene namesi
Name:IST2
Ordered Locus Names:YBR086C
ORF Names:YBR0809
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR086C.
SGDiS000000290. IST2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 121CytoplasmicSequence analysisAdd BLAST121
Transmembranei122 – 142HelicalSequence analysisAdd BLAST21
Topological domaini143 – 153ExtracellularSequence analysisAdd BLAST11
Transmembranei154 – 174HelicalSequence analysisAdd BLAST21
Topological domaini175 – 217CytoplasmicSequence analysisAdd BLAST43
Transmembranei218 – 238HelicalSequence analysisAdd BLAST21
Topological domaini239 – 253ExtracellularSequence analysisAdd BLAST15
Transmembranei254 – 274HelicalSequence analysisAdd BLAST21
Topological domaini275 – 302CytoplasmicSequence analysisAdd BLAST28
Transmembranei303 – 323HelicalSequence analysisAdd BLAST21
Topological domaini324 – 447ExtracellularSequence analysisAdd BLAST124
Transmembranei448 – 468HelicalSequence analysisAdd BLAST21
Topological domaini469 – 505CytoplasmicSequence analysisAdd BLAST37
Transmembranei506 – 526HelicalSequence analysisAdd BLAST21
Topological domaini527 – 563ExtracellularSequence analysisAdd BLAST37
Transmembranei564 – 584HelicalSequence analysisAdd BLAST21
Topological domaini585 – 946CytoplasmicSequence analysisAdd BLAST362

GO - Cellular componenti

  • cellular bud membrane Source: SGD
  • cortical endoplasmic reticulum Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000842611 – 946Increased sodium tolerance protein 2Add BLAST946

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei638PhosphoserineCombined sources1
Modified residuei701PhosphothreonineCombined sources1
Modified residuei704PhosphoserineCombined sources1
Modified residuei720PhosphoserineCombined sources1
Modified residuei726PhosphothreonineCombined sources1
Modified residuei729PhosphoserineCombined sources1
Modified residuei730PhosphotyrosineCombined sources1
Modified residuei757PhosphoserineCombined sources1
Modified residuei793PhosphoserineCombined sources1
Modified residuei844PhosphoserineCombined sources1
Modified residuei847PhosphoserineCombined sources1
Modified residuei850PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38250.
PRIDEiP38250.

PTM databases

iPTMnetiP38250.

Interactioni

Subunit structurei

Interacts with BTN2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BTN2P532862EBI-21520,EBI-3796

Protein-protein interaction databases

BioGridi32792. 59 interactors.
DIPiDIP-6714N.
IntActiP38250. 8 interactors.
MINTiMINT-662530.

Structurei

3D structure databases

ProteinModelPortaliP38250.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiP38250.
KOiK19327.
OMAiMFSTIWP.
OrthoDBiEOG092C0ICW.

Family and domain databases

InterProiIPR007632. Anoctamin.
[Graphical view]
PANTHERiPTHR12308. PTHR12308. 3 hits.
PfamiPF04547. Anoctamin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38250-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQTITSLDP NCVIVFNKTS SANEKSLNVE FKRLNIHSII EPGHDLQTSY
60 70 80 90 100
AFIRIHQDNA KPLFSFLQNL DFIESIIPYH DTELSDDLHK LISISKSKIL
110 120 130 140 150
EAPKQYELYN LSNLTNNPKQ SLYFAFLQNY IKWLIPFSFF GLSIRFLSNF
160 170 180 190 200
TYEFNSTYSL FAILWTLSFT AFWLYKYEPF WSDRLSKYSS FSTIEFLQDK
210 220 230 240 250
QKAQKKASSV IMLKKCCFIP VALLFGAILL SFQLYCFALE IFIKQIYNGP
260 270 280 290 300
MISILSFLPT ILICTFTPVL TVIYNKYFVE PMTKWENHSS VVNAKKSKEA
310 320 330 340 350
KNFVIIFLSS YVPLLITLFL YLPMGHLLTA EIRTKVFNAF SILARLPTHD
360 370 380 390 400
SDFIIDTKRY EDQFFYFIVI NQLIQFSMEN FVPSLVSIAQ QKINGPNPNF
410 420 430 440 450
VKAESEIGKA QLSSSDMKIW SKVKSYQTDP WGATFDLDAN FKKLLLQFGY
460 470 480 490 500
LVMFSTIWPL APFICLIVNL IVYQVDLRKA VLYSKPEYFP FPIYDKPSSV
510 520 530 540 550
SNTQKLTVGL WNSVLVMFSI LGCVITATLT YMYQSCNIPG VGAHTSIHTN
560 570 580 590 600
KAWYLANPIN HSWINIVLYA VFIEHVSVAI FFLFSSILKS SHDDVANGIV
610 620 630 640 650
PKHVVNVQNP PKQEVFEKIP SPEFNSNNEK ELVQRKGSAN EKLHQELGEK
660 670 680 690 700
QPASSANGYE AHAATHANND PSSLSSASSP SLSSSSSSSK TGVVKAVDND
710 720 730 740 750
TAGSAGKKPL ATESTEKRNS LVKVPTVGSY GVAGATLPET IPTSKNYYLR
760 770 780 790 800
FDEDGKSIRD AKSSAESSNA TNNNTLGTES KLLPDGDAVD ALSRKIDQIP
810 820 830 840 850
KIAVTGGENN ENTQAKDDAA TKTPLIKDAN IKPVVNAAVN DNQSKVSVAT
860 870 880 890 900
EQTKKTEVST KNGPSRSIST KETKDSARPS NNNTTTTTTT DATQPHHHHH
910 920 930 940
HHRHRDAGVK NVTNNSKTTE SSSSSSAAKE KPKHKKGLLH KLKKKL
Length:946
Mass (Da):105,854
Last modified:November 2, 2010 - v2
Checksum:i79E90CA4DF89585F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti243I → Y in CAA55593 (PubMed:7900426).Curated1
Sequence conflicti243I → Y in CAA85034 (PubMed:7813418).Curated1
Sequence conflicti243I → Y in CAA85037 (PubMed:7813418).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78993 Genomic DNA. Translation: CAA55593.1.
Z35955 Genomic DNA. Translation: CAA85034.1.
Z35956 Genomic DNA. Translation: CAA85037.1.
BK006936 Genomic DNA. Translation: DAA07207.1.
PIRiS48255.
RefSeqiNP_009643.2. NM_001178434.1.

Genome annotation databases

EnsemblFungiiYBR086C; YBR086C; YBR086C.
GeneIDi852382.
KEGGisce:YBR086C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78993 Genomic DNA. Translation: CAA55593.1.
Z35955 Genomic DNA. Translation: CAA85034.1.
Z35956 Genomic DNA. Translation: CAA85037.1.
BK006936 Genomic DNA. Translation: DAA07207.1.
PIRiS48255.
RefSeqiNP_009643.2. NM_001178434.1.

3D structure databases

ProteinModelPortaliP38250.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32792. 59 interactors.
DIPiDIP-6714N.
IntActiP38250. 8 interactors.
MINTiMINT-662530.

Protein family/group databases

TCDBi1.A.17.1.19. the calcium-dependent chloride channel (ca-clc) family.

PTM databases

iPTMnetiP38250.

Proteomic databases

MaxQBiP38250.
PRIDEiP38250.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR086C; YBR086C; YBR086C.
GeneIDi852382.
KEGGisce:YBR086C.

Organism-specific databases

EuPathDBiFungiDB:YBR086C.
SGDiS000000290. IST2.

Phylogenomic databases

InParanoidiP38250.
KOiK19327.
OMAiMFSTIWP.
OrthoDBiEOG092C0ICW.

Enzyme and pathway databases

BioCyciYEAST:G3O-29053-MONOMER.

Miscellaneous databases

PROiP38250.

Family and domain databases

InterProiIPR007632. Anoctamin.
[Graphical view]
PANTHERiPTHR12308. PTHR12308. 3 hits.
PfamiPF04547. Anoctamin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIST2_YEAST
AccessioniPrimary (citable) accession number: P38250
Secondary accession number(s): D6VQ87, P89499
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 2, 2010
Last modified: November 2, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.