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P38250

- IST2_YEAST

UniProt

P38250 - IST2_YEAST

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Protein

Increased sodium tolerance protein 2

Gene

IST2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be involved in ion homeostasis together with BTN1 or BTN2.1 Publication

GO - Molecular functioni

  1. lipid binding Source: SGD

GO - Biological processi

  1. endoplasmic reticulum membrane organization Source: SGD
  2. protein localization to plasma membrane Source: SGD
  3. regulation of phosphatidylinositol dephosphorylation Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29053-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Increased sodium tolerance protein 2
Gene namesi
Name:IST2
Ordered Locus Names:YBR086C
ORF Names:YBR0809
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

SGDiS000000290. IST2.

Subcellular locationi

Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications
Note: Correct localization requires BTN2. Localizes to the mother cell in small budded cells and to the bud in medium and large budded cells. Transported to the bud tip by an actomyosin based process. Compartmentalization maintained by a septin mediated membrane diffusion barrier at the mother-bud neck.

GO - Cellular componenti

  1. cellular bud membrane Source: SGD
  2. cortical endoplasmic reticulum Source: SGD
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 946946Increased sodium tolerance protein 2PRO_0000084261Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei638 – 6381Phosphoserine1 Publication
Modified residuei701 – 7011Phosphothreonine2 Publications
Modified residuei704 – 7041Phosphoserine2 Publications
Modified residuei720 – 7201Phosphoserine2 Publications
Modified residuei726 – 7261Phosphothreonine3 Publications
Modified residuei729 – 7291Phosphoserine2 Publications
Modified residuei730 – 7301Phosphotyrosine1 Publication
Modified residuei757 – 7571Phosphoserine3 Publications
Modified residuei793 – 7931Phosphoserine2 Publications
Modified residuei844 – 8441Phosphoserine1 Publication
Modified residuei847 – 8471Phosphoserine1 Publication
Modified residuei850 – 8501Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38250.
PaxDbiP38250.
PeptideAtlasiP38250.
PRIDEiP38250.

Expressioni

Gene expression databases

GenevestigatoriP38250.

Interactioni

Subunit structurei

Interacts with BTN2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BTN2P532862EBI-21520,EBI-3796

Protein-protein interaction databases

BioGridi32792. 57 interactions.
DIPiDIP-6714N.
IntActiP38250. 8 interactions.
MINTiMINT-662530.
STRINGi4932.YBR086C.

Structurei

3D structure databases

ProteinModelPortaliP38250.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 121121CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini143 – 15311ExtracellularSequence AnalysisAdd
BLAST
Topological domaini175 – 21743CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini239 – 25315ExtracellularSequence AnalysisAdd
BLAST
Topological domaini275 – 30228CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini324 – 447124ExtracellularSequence AnalysisAdd
BLAST
Topological domaini469 – 50537CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini527 – 56337ExtracellularSequence AnalysisAdd
BLAST
Topological domaini585 – 946362CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei122 – 14221HelicalSequence AnalysisAdd
BLAST
Transmembranei154 – 17421HelicalSequence AnalysisAdd
BLAST
Transmembranei218 – 23821HelicalSequence AnalysisAdd
BLAST
Transmembranei254 – 27421HelicalSequence AnalysisAdd
BLAST
Transmembranei303 – 32321HelicalSequence AnalysisAdd
BLAST
Transmembranei448 – 46821HelicalSequence AnalysisAdd
BLAST
Transmembranei506 – 52621HelicalSequence AnalysisAdd
BLAST
Transmembranei564 – 58421HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG307842.
InParanoidiP38250.
OMAiMFSTIWP.
OrthoDBiEOG7FFN2W.

Family and domain databases

InterProiIPR007632. Anoctamin.
[Graphical view]
PANTHERiPTHR12308. PTHR12308. 1 hit.
PfamiPF04547. Anoctamin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38250 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQTITSLDP NCVIVFNKTS SANEKSLNVE FKRLNIHSII EPGHDLQTSY
60 70 80 90 100
AFIRIHQDNA KPLFSFLQNL DFIESIIPYH DTELSDDLHK LISISKSKIL
110 120 130 140 150
EAPKQYELYN LSNLTNNPKQ SLYFAFLQNY IKWLIPFSFF GLSIRFLSNF
160 170 180 190 200
TYEFNSTYSL FAILWTLSFT AFWLYKYEPF WSDRLSKYSS FSTIEFLQDK
210 220 230 240 250
QKAQKKASSV IMLKKCCFIP VALLFGAILL SFQLYCFALE IFIKQIYNGP
260 270 280 290 300
MISILSFLPT ILICTFTPVL TVIYNKYFVE PMTKWENHSS VVNAKKSKEA
310 320 330 340 350
KNFVIIFLSS YVPLLITLFL YLPMGHLLTA EIRTKVFNAF SILARLPTHD
360 370 380 390 400
SDFIIDTKRY EDQFFYFIVI NQLIQFSMEN FVPSLVSIAQ QKINGPNPNF
410 420 430 440 450
VKAESEIGKA QLSSSDMKIW SKVKSYQTDP WGATFDLDAN FKKLLLQFGY
460 470 480 490 500
LVMFSTIWPL APFICLIVNL IVYQVDLRKA VLYSKPEYFP FPIYDKPSSV
510 520 530 540 550
SNTQKLTVGL WNSVLVMFSI LGCVITATLT YMYQSCNIPG VGAHTSIHTN
560 570 580 590 600
KAWYLANPIN HSWINIVLYA VFIEHVSVAI FFLFSSILKS SHDDVANGIV
610 620 630 640 650
PKHVVNVQNP PKQEVFEKIP SPEFNSNNEK ELVQRKGSAN EKLHQELGEK
660 670 680 690 700
QPASSANGYE AHAATHANND PSSLSSASSP SLSSSSSSSK TGVVKAVDND
710 720 730 740 750
TAGSAGKKPL ATESTEKRNS LVKVPTVGSY GVAGATLPET IPTSKNYYLR
760 770 780 790 800
FDEDGKSIRD AKSSAESSNA TNNNTLGTES KLLPDGDAVD ALSRKIDQIP
810 820 830 840 850
KIAVTGGENN ENTQAKDDAA TKTPLIKDAN IKPVVNAAVN DNQSKVSVAT
860 870 880 890 900
EQTKKTEVST KNGPSRSIST KETKDSARPS NNNTTTTTTT DATQPHHHHH
910 920 930 940
HHRHRDAGVK NVTNNSKTTE SSSSSSAAKE KPKHKKGLLH KLKKKL
Length:946
Mass (Da):105,854
Last modified:November 2, 2010 - v2
Checksum:i79E90CA4DF89585F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti243 – 2431I → Y in CAA55593. (PubMed:7900426)Curated
Sequence conflicti243 – 2431I → Y in CAA85034. (PubMed:7813418)Curated
Sequence conflicti243 – 2431I → Y in CAA85037. (PubMed:7813418)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78993 Genomic DNA. Translation: CAA55593.1.
Z35955 Genomic DNA. Translation: CAA85034.1.
Z35956 Genomic DNA. Translation: CAA85037.1.
BK006936 Genomic DNA. Translation: DAA07207.1.
PIRiS48255.
RefSeqiNP_009643.2. NM_001178434.1.

Genome annotation databases

EnsemblFungiiYBR086C; YBR086C; YBR086C.
GeneIDi852382.
KEGGisce:YBR086C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78993 Genomic DNA. Translation: CAA55593.1 .
Z35955 Genomic DNA. Translation: CAA85034.1 .
Z35956 Genomic DNA. Translation: CAA85037.1 .
BK006936 Genomic DNA. Translation: DAA07207.1 .
PIRi S48255.
RefSeqi NP_009643.2. NM_001178434.1.

3D structure databases

ProteinModelPortali P38250.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32792. 57 interactions.
DIPi DIP-6714N.
IntActi P38250. 8 interactions.
MINTi MINT-662530.
STRINGi 4932.YBR086C.

Proteomic databases

MaxQBi P38250.
PaxDbi P38250.
PeptideAtlasi P38250.
PRIDEi P38250.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR086C ; YBR086C ; YBR086C .
GeneIDi 852382.
KEGGi sce:YBR086C.

Organism-specific databases

SGDi S000000290. IST2.

Phylogenomic databases

eggNOGi NOG307842.
InParanoidi P38250.
OMAi MFSTIWP.
OrthoDBi EOG7FFN2W.

Enzyme and pathway databases

BioCyci YEAST:G3O-29053-MONOMER.

Miscellaneous databases

NextBioi 971186.

Gene expression databases

Genevestigatori P38250.

Family and domain databases

InterProi IPR007632. Anoctamin.
[Graphical view ]
PANTHERi PTHR12308. PTHR12308. 1 hit.
Pfami PF04547. Anoctamin. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
    Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
    Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 243.
    Strain: ATCC 204508 / S288c.
  4. "Interaction among Btn1p, Btn2p, and Ist2p reveals potential interplay among the vacuole, amino acid levels, and ion homeostasis in the yeast Saccharomyces cerevisiae."
    Kim Y., Chattopadhyay S., Locke S., Pearce D.A.
    Eukaryot. Cell 4:281-288(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BTN2, SUBCELLULAR LOCATION.
  5. "Plasma membrane compartmentalization in yeast by messenger RNA transport and a septin diffusion barrier."
    Takizawa P.A., DeRisi J.L., Wilhelm J.E., Vale R.D.
    Science 290:341-344(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-701; SER-704; SER-720; THR-726; SER-729 AND SER-757, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-638 AND SER-757, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-726; SER-757 AND SER-793, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-701; SER-704; SER-720; THR-726; SER-729; TYR-730; SER-793; SER-844; SER-847 AND THR-850, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIST2_YEAST
AccessioniPrimary (citable) accession number: P38250
Secondary accession number(s): D6VQ87, P89499
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 2, 2010
Last modified: October 29, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3