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P38250

- IST2_YEAST

UniProt

P38250 - IST2_YEAST

Protein

Increased sodium tolerance protein 2

Gene

IST2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (02 Nov 2010)
      Previous versions | rss
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    Functioni

    May be involved in ion homeostasis together with BTN1 or BTN2.1 Publication

    GO - Molecular functioni

    1. lipid binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. endoplasmic reticulum membrane organization Source: SGD
    2. protein localization to plasma membrane Source: SGD
    3. regulation of phosphatidylinositol dephosphorylation Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29053-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Increased sodium tolerance protein 2
    Gene namesi
    Name:IST2
    Ordered Locus Names:YBR086C
    ORF Names:YBR0809
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    SGDiS000000290. IST2.

    Subcellular locationi

    Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications
    Note: Correct localization requires BTN2. Localizes to the mother cell in small budded cells and to the bud in medium and large budded cells. Transported to the bud tip by an actomyosin based process. Compartmentalization maintained by a septin mediated membrane diffusion barrier at the mother-bud neck.

    GO - Cellular componenti

    1. cellular bud membrane Source: SGD
    2. cortical endoplasmic reticulum Source: SGD
    3. integral component of membrane Source: UniProtKB-KW
    4. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 946946Increased sodium tolerance protein 2PRO_0000084261Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei638 – 6381Phosphoserine1 Publication
    Modified residuei701 – 7011Phosphothreonine2 Publications
    Modified residuei704 – 7041Phosphoserine2 Publications
    Modified residuei720 – 7201Phosphoserine2 Publications
    Modified residuei726 – 7261Phosphothreonine3 Publications
    Modified residuei729 – 7291Phosphoserine2 Publications
    Modified residuei730 – 7301Phosphotyrosine1 Publication
    Modified residuei757 – 7571Phosphoserine3 Publications
    Modified residuei793 – 7931Phosphoserine2 Publications
    Modified residuei844 – 8441Phosphoserine1 Publication
    Modified residuei847 – 8471Phosphoserine1 Publication
    Modified residuei850 – 8501Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38250.
    PaxDbiP38250.
    PeptideAtlasiP38250.
    PRIDEiP38250.

    Expressioni

    Gene expression databases

    GenevestigatoriP38250.

    Interactioni

    Subunit structurei

    Interacts with BTN2.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BTN2P532862EBI-21520,EBI-3796

    Protein-protein interaction databases

    BioGridi32792. 57 interactions.
    DIPiDIP-6714N.
    IntActiP38250. 8 interactions.
    MINTiMINT-662530.
    STRINGi4932.YBR086C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38250.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 121121CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini143 – 15311ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini175 – 21743CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini239 – 25315ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini275 – 30228CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini324 – 447124ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini469 – 50537CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini527 – 56337ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini585 – 946362CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei122 – 14221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei154 – 17421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei218 – 23821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei254 – 27421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei303 – 32321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei448 – 46821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei506 – 52621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei564 – 58421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG307842.
    OMAiMFSTIWP.
    OrthoDBiEOG7FFN2W.

    Family and domain databases

    InterProiIPR007632. Anoctamin.
    [Graphical view]
    PANTHERiPTHR12308. PTHR12308. 1 hit.
    PfamiPF04547. Anoctamin. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38250-1 [UniParc]FASTAAdd to Basket

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    MSQTITSLDP NCVIVFNKTS SANEKSLNVE FKRLNIHSII EPGHDLQTSY    50
    AFIRIHQDNA KPLFSFLQNL DFIESIIPYH DTELSDDLHK LISISKSKIL 100
    EAPKQYELYN LSNLTNNPKQ SLYFAFLQNY IKWLIPFSFF GLSIRFLSNF 150
    TYEFNSTYSL FAILWTLSFT AFWLYKYEPF WSDRLSKYSS FSTIEFLQDK 200
    QKAQKKASSV IMLKKCCFIP VALLFGAILL SFQLYCFALE IFIKQIYNGP 250
    MISILSFLPT ILICTFTPVL TVIYNKYFVE PMTKWENHSS VVNAKKSKEA 300
    KNFVIIFLSS YVPLLITLFL YLPMGHLLTA EIRTKVFNAF SILARLPTHD 350
    SDFIIDTKRY EDQFFYFIVI NQLIQFSMEN FVPSLVSIAQ QKINGPNPNF 400
    VKAESEIGKA QLSSSDMKIW SKVKSYQTDP WGATFDLDAN FKKLLLQFGY 450
    LVMFSTIWPL APFICLIVNL IVYQVDLRKA VLYSKPEYFP FPIYDKPSSV 500
    SNTQKLTVGL WNSVLVMFSI LGCVITATLT YMYQSCNIPG VGAHTSIHTN 550
    KAWYLANPIN HSWINIVLYA VFIEHVSVAI FFLFSSILKS SHDDVANGIV 600
    PKHVVNVQNP PKQEVFEKIP SPEFNSNNEK ELVQRKGSAN EKLHQELGEK 650
    QPASSANGYE AHAATHANND PSSLSSASSP SLSSSSSSSK TGVVKAVDND 700
    TAGSAGKKPL ATESTEKRNS LVKVPTVGSY GVAGATLPET IPTSKNYYLR 750
    FDEDGKSIRD AKSSAESSNA TNNNTLGTES KLLPDGDAVD ALSRKIDQIP 800
    KIAVTGGENN ENTQAKDDAA TKTPLIKDAN IKPVVNAAVN DNQSKVSVAT 850
    EQTKKTEVST KNGPSRSIST KETKDSARPS NNNTTTTTTT DATQPHHHHH 900
    HHRHRDAGVK NVTNNSKTTE SSSSSSAAKE KPKHKKGLLH KLKKKL 946
    Length:946
    Mass (Da):105,854
    Last modified:November 2, 2010 - v2
    Checksum:i79E90CA4DF89585F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti243 – 2431I → Y in CAA55593. (PubMed:7900426)Curated
    Sequence conflicti243 – 2431I → Y in CAA85034. (PubMed:7813418)Curated
    Sequence conflicti243 – 2431I → Y in CAA85037. (PubMed:7813418)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78993 Genomic DNA. Translation: CAA55593.1.
    Z35955 Genomic DNA. Translation: CAA85034.1.
    Z35956 Genomic DNA. Translation: CAA85037.1.
    BK006936 Genomic DNA. Translation: DAA07207.1.
    PIRiS48255.
    RefSeqiNP_009643.2. NM_001178434.1.

    Genome annotation databases

    EnsemblFungiiYBR086C; YBR086C; YBR086C.
    GeneIDi852382.
    KEGGisce:YBR086C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78993 Genomic DNA. Translation: CAA55593.1 .
    Z35955 Genomic DNA. Translation: CAA85034.1 .
    Z35956 Genomic DNA. Translation: CAA85037.1 .
    BK006936 Genomic DNA. Translation: DAA07207.1 .
    PIRi S48255.
    RefSeqi NP_009643.2. NM_001178434.1.

    3D structure databases

    ProteinModelPortali P38250.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32792. 57 interactions.
    DIPi DIP-6714N.
    IntActi P38250. 8 interactions.
    MINTi MINT-662530.
    STRINGi 4932.YBR086C.

    Proteomic databases

    MaxQBi P38250.
    PaxDbi P38250.
    PeptideAtlasi P38250.
    PRIDEi P38250.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR086C ; YBR086C ; YBR086C .
    GeneIDi 852382.
    KEGGi sce:YBR086C.

    Organism-specific databases

    SGDi S000000290. IST2.

    Phylogenomic databases

    eggNOGi NOG307842.
    OMAi MFSTIWP.
    OrthoDBi EOG7FFN2W.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29053-MONOMER.

    Miscellaneous databases

    NextBioi 971186.

    Gene expression databases

    Genevestigatori P38250.

    Family and domain databases

    InterProi IPR007632. Anoctamin.
    [Graphical view ]
    PANTHERi PTHR12308. PTHR12308. 1 hit.
    Pfami PF04547. Anoctamin. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
      Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
      Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 243.
      Strain: ATCC 204508 / S288c.
    4. "Interaction among Btn1p, Btn2p, and Ist2p reveals potential interplay among the vacuole, amino acid levels, and ion homeostasis in the yeast Saccharomyces cerevisiae."
      Kim Y., Chattopadhyay S., Locke S., Pearce D.A.
      Eukaryot. Cell 4:281-288(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH BTN2, SUBCELLULAR LOCATION.
    5. "Plasma membrane compartmentalization in yeast by messenger RNA transport and a septin diffusion barrier."
      Takizawa P.A., DeRisi J.L., Wilhelm J.E., Vale R.D.
      Science 290:341-344(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-701; SER-704; SER-720; THR-726; SER-729 AND SER-757, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-638 AND SER-757, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-726; SER-757 AND SER-793, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-701; SER-704; SER-720; THR-726; SER-729; TYR-730; SER-793; SER-844; SER-847 AND THR-850, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiIST2_YEAST
    AccessioniPrimary (citable) accession number: P38250
    Secondary accession number(s): D6VQ87, P89499
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: November 2, 2010
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3