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P38250

- IST2_YEAST

UniProt

P38250 - IST2_YEAST

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Protein
Increased sodium tolerance protein 2
Gene
IST2, YBR086C, YBR0809
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May be involved in ion homeostasis together with BTN1 or BTN2.1 Publication

GO - Molecular functioni

  1. lipid binding Source: SGD
  2. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. endoplasmic reticulum membrane organization Source: SGD
  2. protein localization to plasma membrane Source: SGD
  3. regulation of phosphatidylinositol dephosphorylation Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29053-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Increased sodium tolerance protein 2
Gene namesi
Name:IST2
Ordered Locus Names:YBR086C
ORF Names:YBR0809
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

SGDiS000000290. IST2.

Subcellular locationi

Cell membrane; Multi-pass membrane protein
Note: Correct localization requires BTN2. Localizes to the mother cell in small budded cells and to the bud in medium and large budded cells. Transported to the bud tip by an actomyosin based process. Compartmentalization maintained by a septin mediated membrane diffusion barrier at the mother-bud neck.2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 121121Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei122 – 14221Helical; Reviewed prediction
Add
BLAST
Topological domaini143 – 15311Extracellular Reviewed prediction
Add
BLAST
Transmembranei154 – 17421Helical; Reviewed prediction
Add
BLAST
Topological domaini175 – 21743Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei218 – 23821Helical; Reviewed prediction
Add
BLAST
Topological domaini239 – 25315Extracellular Reviewed prediction
Add
BLAST
Transmembranei254 – 27421Helical; Reviewed prediction
Add
BLAST
Topological domaini275 – 30228Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei303 – 32321Helical; Reviewed prediction
Add
BLAST
Topological domaini324 – 447124Extracellular Reviewed prediction
Add
BLAST
Transmembranei448 – 46821Helical; Reviewed prediction
Add
BLAST
Topological domaini469 – 50537Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei506 – 52621Helical; Reviewed prediction
Add
BLAST
Topological domaini527 – 56337Extracellular Reviewed prediction
Add
BLAST
Transmembranei564 – 58421Helical; Reviewed prediction
Add
BLAST
Topological domaini585 – 946362Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cellular bud membrane Source: SGD
  2. cortical endoplasmic reticulum Source: SGD
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 946946Increased sodium tolerance protein 2
PRO_0000084261Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei638 – 6381Phosphoserine1 Publication
Modified residuei701 – 7011Phosphothreonine2 Publications
Modified residuei704 – 7041Phosphoserine2 Publications
Modified residuei720 – 7201Phosphoserine2 Publications
Modified residuei726 – 7261Phosphothreonine3 Publications
Modified residuei729 – 7291Phosphoserine2 Publications
Modified residuei730 – 7301Phosphotyrosine1 Publication
Modified residuei757 – 7571Phosphoserine3 Publications
Modified residuei793 – 7931Phosphoserine2 Publications
Modified residuei844 – 8441Phosphoserine1 Publication
Modified residuei847 – 8471Phosphoserine1 Publication
Modified residuei850 – 8501Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38250.
PaxDbiP38250.
PeptideAtlasiP38250.
PRIDEiP38250.

Expressioni

Gene expression databases

GenevestigatoriP38250.

Interactioni

Subunit structurei

Interacts with BTN2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BTN2P532862EBI-21520,EBI-3796

Protein-protein interaction databases

BioGridi32792. 57 interactions.
DIPiDIP-6714N.
IntActiP38250. 8 interactions.
MINTiMINT-662530.
STRINGi4932.YBR086C.

Structurei

3D structure databases

ProteinModelPortaliP38250.

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG307842.
OMAiMFSTIWP.
OrthoDBiEOG7FFN2W.

Family and domain databases

InterProiIPR007632. Anoctamin.
[Graphical view]
PANTHERiPTHR12308. PTHR12308. 1 hit.
PfamiPF04547. Anoctamin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38250-1 [UniParc]FASTAAdd to Basket

« Hide

MSQTITSLDP NCVIVFNKTS SANEKSLNVE FKRLNIHSII EPGHDLQTSY    50
AFIRIHQDNA KPLFSFLQNL DFIESIIPYH DTELSDDLHK LISISKSKIL 100
EAPKQYELYN LSNLTNNPKQ SLYFAFLQNY IKWLIPFSFF GLSIRFLSNF 150
TYEFNSTYSL FAILWTLSFT AFWLYKYEPF WSDRLSKYSS FSTIEFLQDK 200
QKAQKKASSV IMLKKCCFIP VALLFGAILL SFQLYCFALE IFIKQIYNGP 250
MISILSFLPT ILICTFTPVL TVIYNKYFVE PMTKWENHSS VVNAKKSKEA 300
KNFVIIFLSS YVPLLITLFL YLPMGHLLTA EIRTKVFNAF SILARLPTHD 350
SDFIIDTKRY EDQFFYFIVI NQLIQFSMEN FVPSLVSIAQ QKINGPNPNF 400
VKAESEIGKA QLSSSDMKIW SKVKSYQTDP WGATFDLDAN FKKLLLQFGY 450
LVMFSTIWPL APFICLIVNL IVYQVDLRKA VLYSKPEYFP FPIYDKPSSV 500
SNTQKLTVGL WNSVLVMFSI LGCVITATLT YMYQSCNIPG VGAHTSIHTN 550
KAWYLANPIN HSWINIVLYA VFIEHVSVAI FFLFSSILKS SHDDVANGIV 600
PKHVVNVQNP PKQEVFEKIP SPEFNSNNEK ELVQRKGSAN EKLHQELGEK 650
QPASSANGYE AHAATHANND PSSLSSASSP SLSSSSSSSK TGVVKAVDND 700
TAGSAGKKPL ATESTEKRNS LVKVPTVGSY GVAGATLPET IPTSKNYYLR 750
FDEDGKSIRD AKSSAESSNA TNNNTLGTES KLLPDGDAVD ALSRKIDQIP 800
KIAVTGGENN ENTQAKDDAA TKTPLIKDAN IKPVVNAAVN DNQSKVSVAT 850
EQTKKTEVST KNGPSRSIST KETKDSARPS NNNTTTTTTT DATQPHHHHH 900
HHRHRDAGVK NVTNNSKTTE SSSSSSAAKE KPKHKKGLLH KLKKKL 946
Length:946
Mass (Da):105,854
Last modified:November 2, 2010 - v2
Checksum:i79E90CA4DF89585F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti243 – 2431I → Y in CAA55593. 1 Publication
Sequence conflicti243 – 2431I → Y in CAA85034. 1 Publication
Sequence conflicti243 – 2431I → Y in CAA85037. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78993 Genomic DNA. Translation: CAA55593.1.
Z35955 Genomic DNA. Translation: CAA85034.1.
Z35956 Genomic DNA. Translation: CAA85037.1.
BK006936 Genomic DNA. Translation: DAA07207.1.
PIRiS48255.
RefSeqiNP_009643.2. NM_001178434.1.

Genome annotation databases

EnsemblFungiiYBR086C; YBR086C; YBR086C.
GeneIDi852382.
KEGGisce:YBR086C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78993 Genomic DNA. Translation: CAA55593.1 .
Z35955 Genomic DNA. Translation: CAA85034.1 .
Z35956 Genomic DNA. Translation: CAA85037.1 .
BK006936 Genomic DNA. Translation: DAA07207.1 .
PIRi S48255.
RefSeqi NP_009643.2. NM_001178434.1.

3D structure databases

ProteinModelPortali P38250.
ModBasei Search...

Protein-protein interaction databases

BioGridi 32792. 57 interactions.
DIPi DIP-6714N.
IntActi P38250. 8 interactions.
MINTi MINT-662530.
STRINGi 4932.YBR086C.

Proteomic databases

MaxQBi P38250.
PaxDbi P38250.
PeptideAtlasi P38250.
PRIDEi P38250.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR086C ; YBR086C ; YBR086C .
GeneIDi 852382.
KEGGi sce:YBR086C.

Organism-specific databases

SGDi S000000290. IST2.

Phylogenomic databases

eggNOGi NOG307842.
OMAi MFSTIWP.
OrthoDBi EOG7FFN2W.

Enzyme and pathway databases

BioCyci YEAST:G3O-29053-MONOMER.

Miscellaneous databases

NextBioi 971186.

Gene expression databases

Genevestigatori P38250.

Family and domain databases

InterProi IPR007632. Anoctamin.
[Graphical view ]
PANTHERi PTHR12308. PTHR12308. 1 hit.
Pfami PF04547. Anoctamin. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
    Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
    Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 243.
    Strain: ATCC 204508 / S288c.
  4. "Interaction among Btn1p, Btn2p, and Ist2p reveals potential interplay among the vacuole, amino acid levels, and ion homeostasis in the yeast Saccharomyces cerevisiae."
    Kim Y., Chattopadhyay S., Locke S., Pearce D.A.
    Eukaryot. Cell 4:281-288(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BTN2, SUBCELLULAR LOCATION.
  5. "Plasma membrane compartmentalization in yeast by messenger RNA transport and a septin diffusion barrier."
    Takizawa P.A., DeRisi J.L., Wilhelm J.E., Vale R.D.
    Science 290:341-344(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-701; SER-704; SER-720; THR-726; SER-729 AND SER-757, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-638 AND SER-757, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-726; SER-757 AND SER-793, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-701; SER-704; SER-720; THR-726; SER-729; TYR-730; SER-793; SER-844; SER-847 AND THR-850, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIST2_YEAST
AccessioniPrimary (citable) accession number: P38250
Secondary accession number(s): D6VQ87, P89499
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 2, 2010
Last modified: September 3, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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