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P38250 (IST2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Increased sodium tolerance protein 2
Gene names
Name:IST2
Ordered Locus Names:YBR086C
ORF Names:YBR0809
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length946 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in ion homeostasis together with BTN1 or BTN2. Ref.4

Subunit structure

Interacts with BTN2. Ref.4

Subcellular location

Cell membrane; Multi-pass membrane protein. Note: Correct localization requires BTN2. Localizes to the mother cell in small budded cells and to the bud in medium and large budded cells. Transported to the bud tip by an actomyosin based process. Compartmentalization maintained by a septin mediated membrane diffusion barrier at the mother-bud neck. Ref.4 Ref.5

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BTN2P532862EBI-21520,EBI-3796

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 946946Increased sodium tolerance protein 2
PRO_0000084261

Regions

Topological domain1 – 121121Cytoplasmic Potential
Transmembrane122 – 14221Helical; Potential
Topological domain143 – 15311Extracellular Potential
Transmembrane154 – 17421Helical; Potential
Topological domain175 – 21743Cytoplasmic Potential
Transmembrane218 – 23821Helical; Potential
Topological domain239 – 25315Extracellular Potential
Transmembrane254 – 27421Helical; Potential
Topological domain275 – 30228Cytoplasmic Potential
Transmembrane303 – 32321Helical; Potential
Topological domain324 – 447124Extracellular Potential
Transmembrane448 – 46821Helical; Potential
Topological domain469 – 50537Cytoplasmic Potential
Transmembrane506 – 52621Helical; Potential
Topological domain527 – 56337Extracellular Potential
Transmembrane564 – 58421Helical; Potential
Topological domain585 – 946362Cytoplasmic Potential

Amino acid modifications

Modified residue6381Phosphoserine Ref.9
Modified residue7011Phosphothreonine Ref.8 Ref.11
Modified residue7041Phosphoserine Ref.8 Ref.11
Modified residue7201Phosphoserine Ref.8 Ref.11
Modified residue7261Phosphothreonine Ref.8 Ref.10 Ref.11
Modified residue7291Phosphoserine Ref.8 Ref.11
Modified residue7301Phosphotyrosine Ref.11
Modified residue7571Phosphoserine Ref.8 Ref.9 Ref.10
Modified residue7931Phosphoserine Ref.10 Ref.11
Modified residue8441Phosphoserine Ref.11
Modified residue8471Phosphoserine Ref.11
Modified residue8501Phosphothreonine Ref.11

Experimental info

Sequence conflict2431I → Y in CAA55593. Ref.1
Sequence conflict2431I → Y in CAA85034. Ref.2
Sequence conflict2431I → Y in CAA85037. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P38250 [UniParc].

Last modified November 2, 2010. Version 2.
Checksum: 79E90CA4DF89585F

FASTA946105,854
        10         20         30         40         50         60 
MSQTITSLDP NCVIVFNKTS SANEKSLNVE FKRLNIHSII EPGHDLQTSY AFIRIHQDNA 

        70         80         90        100        110        120 
KPLFSFLQNL DFIESIIPYH DTELSDDLHK LISISKSKIL EAPKQYELYN LSNLTNNPKQ 

       130        140        150        160        170        180 
SLYFAFLQNY IKWLIPFSFF GLSIRFLSNF TYEFNSTYSL FAILWTLSFT AFWLYKYEPF 

       190        200        210        220        230        240 
WSDRLSKYSS FSTIEFLQDK QKAQKKASSV IMLKKCCFIP VALLFGAILL SFQLYCFALE 

       250        260        270        280        290        300 
IFIKQIYNGP MISILSFLPT ILICTFTPVL TVIYNKYFVE PMTKWENHSS VVNAKKSKEA 

       310        320        330        340        350        360 
KNFVIIFLSS YVPLLITLFL YLPMGHLLTA EIRTKVFNAF SILARLPTHD SDFIIDTKRY 

       370        380        390        400        410        420 
EDQFFYFIVI NQLIQFSMEN FVPSLVSIAQ QKINGPNPNF VKAESEIGKA QLSSSDMKIW 

       430        440        450        460        470        480 
SKVKSYQTDP WGATFDLDAN FKKLLLQFGY LVMFSTIWPL APFICLIVNL IVYQVDLRKA 

       490        500        510        520        530        540 
VLYSKPEYFP FPIYDKPSSV SNTQKLTVGL WNSVLVMFSI LGCVITATLT YMYQSCNIPG 

       550        560        570        580        590        600 
VGAHTSIHTN KAWYLANPIN HSWINIVLYA VFIEHVSVAI FFLFSSILKS SHDDVANGIV 

       610        620        630        640        650        660 
PKHVVNVQNP PKQEVFEKIP SPEFNSNNEK ELVQRKGSAN EKLHQELGEK QPASSANGYE 

       670        680        690        700        710        720 
AHAATHANND PSSLSSASSP SLSSSSSSSK TGVVKAVDND TAGSAGKKPL ATESTEKRNS 

       730        740        750        760        770        780 
LVKVPTVGSY GVAGATLPET IPTSKNYYLR FDEDGKSIRD AKSSAESSNA TNNNTLGTES 

       790        800        810        820        830        840 
KLLPDGDAVD ALSRKIDQIP KIAVTGGENN ENTQAKDDAA TKTPLIKDAN IKPVVNAAVN 

       850        860        870        880        890        900 
DNQSKVSVAT EQTKKTEVST KNGPSRSIST KETKDSARPS NNNTTTTTTT DATQPHHHHH 

       910        920        930        940 
HHRHRDAGVK NVTNNSKTTE SSSSSSAAKE KPKHKKGLLH KLKKKL 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of a 70 kb region on the right arm of yeast chromosome II."
Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 243.
Strain: ATCC 204508 / S288c.
[4]"Interaction among Btn1p, Btn2p, and Ist2p reveals potential interplay among the vacuole, amino acid levels, and ion homeostasis in the yeast Saccharomyces cerevisiae."
Kim Y., Chattopadhyay S., Locke S., Pearce D.A.
Eukaryot. Cell 4:281-288(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH BTN2, SUBCELLULAR LOCATION.
[5]"Plasma membrane compartmentalization in yeast by messenger RNA transport and a septin diffusion barrier."
Takizawa P.A., DeRisi J.L., Wilhelm J.E., Vale R.D.
Science 290:341-344(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[7]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-701; SER-704; SER-720; THR-726; SER-729 AND SER-757, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-638 AND SER-757, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-726; SER-757 AND SER-793, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-701; SER-704; SER-720; THR-726; SER-729; TYR-730; SER-793; SER-844; SER-847 AND THR-850, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X78993 Genomic DNA. Translation: CAA55593.1.
Z35955 Genomic DNA. Translation: CAA85034.1.
Z35956 Genomic DNA. Translation: CAA85037.1.
BK006936 Genomic DNA. Translation: DAA07207.1.
PIRS48255.
RefSeqNP_009643.2. NM_001178434.1.

3D structure databases

ProteinModelPortalP38250.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32792. 57 interactions.
DIPDIP-6714N.
IntActP38250. 8 interactions.
MINTMINT-662530.
STRING4932.YBR086C.

Proteomic databases

PaxDbP38250.
PeptideAtlasP38250.
PRIDEP38250.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR086C; YBR086C; YBR086C.
GeneID852382.
KEGGsce:YBR086C.

Organism-specific databases

SGDS000000290. IST2.

Phylogenomic databases

eggNOGNOG307842.
OMAMFSTIWP.
OrthoDBEOG7FFN2W.

Enzyme and pathway databases

BioCycYEAST:G3O-29053-MONOMER.

Gene expression databases

GenevestigatorP38250.

Family and domain databases

InterProIPR007632. Anoctamin.
[Graphical view]
PANTHERPTHR12308. PTHR12308. 1 hit.
PfamPF04547. Anoctamin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio971186.

Entry information

Entry nameIST2_YEAST
AccessionPrimary (citable) accession number: P38250
Secondary accession number(s): D6VQ87, P89499
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 2, 2010
Last modified: April 16, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD