Increased sodium tolerance protein 2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P38250 (IST2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Increased sodium tolerance protein 2

Gene names

Name:	IST2
Ordered Locus Names:	YBR086C
ORF Names:	YBR0809

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	946 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in ion homeostasis together with BTN1 or BTN2. Ref.4
Subunit structure	Interacts with BTN2. Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein. Note: Correct localization requires BTN2. Localizes to the mother cell in small budded cells and to the bud in medium and large budded cells. Transported to the bud tip by an actomyosin based process. Compartmentalization maintained by a septin mediated membrane diffusion barrier at the mother-bud neck. Ref.4 Ref.5

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endoplasmic reticulum membrane organization Inferred from genetic interaction PubMed 23237950. Source: SGD regulation of phosphatidylinositol dephosphorylation Inferred from genetic interaction PubMed 23237950. Source: SGD response to osmotic stress Traceable author statement PubMed 12362983. Source: SGD
Cellular_component	cellular bud membrane Inferred from direct assay Ref.5 PubMed 13679573. Source: SGD cortical endoplasmic reticulum Inferred from direct assay PubMed 23237950. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Molecular_function	lipid binding Inferred from direct assay PubMed 19453974. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BTN2	P53286	2	EBI-21520,EBI-3796

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 946

946

Increased sodium tolerance protein 2

PRO_0000084261

Regions

Topological domain

1 – 121

121

Cytoplasmic Potential

Transmembrane

122 – 142

Helical; Potential

Topological domain

143 – 153

Extracellular Potential

Transmembrane

154 – 174

Helical; Potential

Topological domain

175 – 217

Cytoplasmic Potential

Transmembrane

218 – 238

Helical; Potential

Topological domain

239 – 253

Extracellular Potential

Transmembrane

254 – 274

Helical; Potential

Topological domain

275 – 302

Cytoplasmic Potential

Transmembrane

303 – 323

Helical; Potential

Topological domain

324 – 447

124

Extracellular Potential

Transmembrane

448 – 468

Helical; Potential

Topological domain

469 – 505

Cytoplasmic Potential

Transmembrane

506 – 526

Helical; Potential

Topological domain

527 – 563

Extracellular Potential

Transmembrane

564 – 584

Helical; Potential

Topological domain

585 – 946

362

Cytoplasmic Potential

Amino acid modifications

Modified residue

621

Phosphoserine Ref.12

Modified residue

638

Phosphoserine Ref.10

Modified residue

701

Phosphothreonine Ref.9 Ref.11

Modified residue

704

Phosphoserine Ref.9 Ref.11

Modified residue

714

Phosphoserine Ref.12

Modified residue

720

Phosphoserine Ref.9 Ref.11

Modified residue

726

Phosphothreonine Ref.6 Ref.9 Ref.11 Ref.12

Modified residue

729

Phosphoserine Ref.6 Ref.7 Ref.9 Ref.11 Ref.12

Modified residue

730

Phosphotyrosine Ref.12

Modified residue

757

Phosphoserine Ref.7 Ref.9 Ref.10 Ref.11 Ref.12

Modified residue

767

Phosphoserine Ref.12

Modified residue

768

Phosphoserine Ref.12

Modified residue

793

Phosphoserine Ref.12

Modified residue

823

Phosphothreonine Ref.12

Modified residue

844

Phosphoserine Ref.12

Modified residue

847

Phosphoserine Ref.12

Modified residue

850

Phosphothreonine Ref.11

Modified residue

853

Phosphothreonine Ref.11

Modified residue

856

Phosphothreonine Ref.11

Modified residue

924

Phosphoserine Ref.6

Modified residue

926

Phosphoserine Ref.6

Experimental info

Sequence conflict

243

I → Y in CAA55593. Ref.1

Sequence conflict

243

I → Y in CAA85034. Ref.2

Sequence conflict

243

I → Y in CAA85037. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P38250 [UniParc].

Last modified November 2, 2010. Version 2.
Checksum: 79E90CA4DF89585F
Show »

FASTA

946

105,854

        10         20         30         40         50         60 
MSQTITSLDP NCVIVFNKTS SANEKSLNVE FKRLNIHSII EPGHDLQTSY AFIRIHQDNA 

        70         80         90        100        110        120 
KPLFSFLQNL DFIESIIPYH DTELSDDLHK LISISKSKIL EAPKQYELYN LSNLTNNPKQ 

       130        140        150        160        170        180 
SLYFAFLQNY IKWLIPFSFF GLSIRFLSNF TYEFNSTYSL FAILWTLSFT AFWLYKYEPF 

       190        200        210        220        230        240 
WSDRLSKYSS FSTIEFLQDK QKAQKKASSV IMLKKCCFIP VALLFGAILL SFQLYCFALE 

       250        260        270        280        290        300 
IFIKQIYNGP MISILSFLPT ILICTFTPVL TVIYNKYFVE PMTKWENHSS VVNAKKSKEA 

       310        320        330        340        350        360 
KNFVIIFLSS YVPLLITLFL YLPMGHLLTA EIRTKVFNAF SILARLPTHD SDFIIDTKRY 

       370        380        390        400        410        420 
EDQFFYFIVI NQLIQFSMEN FVPSLVSIAQ QKINGPNPNF VKAESEIGKA QLSSSDMKIW 

       430        440        450        460        470        480 
SKVKSYQTDP WGATFDLDAN FKKLLLQFGY LVMFSTIWPL APFICLIVNL IVYQVDLRKA 

       490        500        510        520        530        540 
VLYSKPEYFP FPIYDKPSSV SNTQKLTVGL WNSVLVMFSI LGCVITATLT YMYQSCNIPG 

       550        560        570        580        590        600 
VGAHTSIHTN KAWYLANPIN HSWINIVLYA VFIEHVSVAI FFLFSSILKS SHDDVANGIV 

       610        620        630        640        650        660 
PKHVVNVQNP PKQEVFEKIP SPEFNSNNEK ELVQRKGSAN EKLHQELGEK QPASSANGYE 

       670        680        690        700        710        720 
AHAATHANND PSSLSSASSP SLSSSSSSSK TGVVKAVDND TAGSAGKKPL ATESTEKRNS 

       730        740        750        760        770        780 
LVKVPTVGSY GVAGATLPET IPTSKNYYLR FDEDGKSIRD AKSSAESSNA TNNNTLGTES 

       790        800        810        820        830        840 
KLLPDGDAVD ALSRKIDQIP KIAVTGGENN ENTQAKDDAA TKTPLIKDAN IKPVVNAAVN 

       850        860        870        880        890        900 
DNQSKVSVAT EQTKKTEVST KNGPSRSIST KETKDSARPS NNNTTTTTTT DATQPHHHHH 

       910        920        930        940 
HHRHRDAGVK NVTNNSKTTE SSSSSSAAKE KPKHKKGLLH KLKKKL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 70 kb region on the right arm of yeast chromosome II." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 243. Strain: ATCC 204508 / S288c.
[4]	"Interaction among Btn1p, Btn2p, and Ist2p reveals potential interplay among the vacuole, amino acid levels, and ion homeostasis in the yeast Saccharomyces cerevisiae." Kim Y., Chattopadhyay S., Locke S., Pearce D.A. Eukaryot. Cell 4:281-288(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH BTN2, SUBCELLULAR LOCATION.
[5]	"Plasma membrane compartmentalization in yeast by messenger RNA transport and a septin diffusion barrier." Takizawa P.A., DeRisi J.L., Wilhelm J.E., Vale R.D. Science 290:341-344(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-726; SER-729; SER-924 AND SER-926, MASS SPECTROMETRY. Strain: 2124.
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-729 AND SER-757, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-701; SER-704; SER-720; THR-726; SER-729 AND SER-757, MASS SPECTROMETRY. Strain: ADR376.
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-638 AND SER-757, MASS SPECTROMETRY.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-701; SER-704; SER-720; THR-726; SER-729; SER-757; THR-850; THR-853 AND THR-856, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-714; THR-726; SER-729; TYR-730; SER-757; SER-767; SER-768; SER-793; THR-823; SER-844 AND SER-847, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78993 Genomic DNA. Translation: CAA55593.1. Z35955 Genomic DNA. Translation: CAA85034.1. Z35956 Genomic DNA. Translation: CAA85037.1. BK006936 Genomic DNA. Translation: DAA07207.1.
PIR	S48255.
RefSeq	NP_009643.2. NM_001178434.1.
3D structure databases
ProteinModelPortal	P38250.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6714N.
IntAct	P38250. 7 interactions.
MINT	MINT-662530.
STRING	4932.YBR086C.
Proteomic databases
PaxDb	P38250.
PeptideAtlas	P38250.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR086C; YBR086C; YBR086C.
GeneID	852382.
KEGG	sce:YBR086C.
Organism-specific databases
SGD	S000000290. IST2.
Phylogenomic databases
eggNOG	NOG307842.
OMA	MFSTIWP.
OrthoDB	EOG41RT38.
Gene expression databases
Genevestigator	P38250.
GermOnline	YBR086C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007632. Anoctamin. [Graphical view]
PANTHER	PTHR12308. PTHR12308. 1 hit.
Pfam	PF04547. Anoctamin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971186.

Entry information

Entry name

IST2_YEAST

Accession

Primary (citable) accession number: P38250
Secondary accession number(s): D6VQ87, P89499

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	November 2, 2010
Last modified:	April 3, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents