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Protein

Cell wall protein ECM33

Gene

ECM33

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for proper cell wall integrity and for the correct assembly of the mannoprotein outer layer of the cell wall. Important for apical bud growth.3 Publications

Miscellaneous

Present with 6500 wall-bound molecules/cell in log phase YPD medium.1 Publication

GO - Biological processi

  • fungal-type cell wall organization Source: SGD

Keywordsi

Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-29046-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Cell wall protein ECM33
Alternative name(s):
Extracellular mutant protein 33
Gene namesi
Name:ECM33
Ordered Locus Names:YBR078W
ORF Names:YBR0727
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR078W
SGDiS000000282 ECM33

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000003319120 – 406Cell wall protein ECM33Add BLAST387
PropeptideiPRO_0000033192407 – 429Removed in mature formSequence analysisAdd BLAST23

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi21N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi56N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi82N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi196N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi209N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi227N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi234N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi241N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi267N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi279N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi304N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi328N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei339PhosphoserineCombined sources1
Glycosylationi389N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi406GPI-anchor amidated glycineSequence analysis1

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.Curated
Extensively N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

MaxQBiP38248
PaxDbiP38248
PRIDEiP38248

PTM databases

iPTMnetiP38248

Interactioni

Protein-protein interaction databases

BioGridi32780, 338 interactors
DIPiDIP-6584N
IntActiP38248, 5 interactors
STRINGi4932.YBR078W

Structurei

3D structure databases

ProteinModelPortaliP38248
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi311 – 404Ser-richAdd BLAST94

Sequence similaritiesi

Belongs to the SPS2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00530000065410
HOGENOMiHOG000093252
InParanoidiP38248
OMAiGGFQIAR
OrthoDBiEOG092C3DSS

Family and domain databases

Gene3Di3.80.20.20, 1 hit
InterProiView protein in InterPro
IPR036941 Rcpt_L-dom_sf

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38248-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFKNALTAT AILSASALAA NSTTSIPSSC SIGTSATATA QADLDKISGC
60 70 80 90 100
STIVGNLTIT GDLGSAALAS IQEIDGSLTI FNSSSLSSFS ADSIKKITGD
110 120 130 140 150
LNMQELIILT SASFGSLQEV DSINMVTLPA ISTFSTDLQN ANNIIVSDTT
160 170 180 190 200
LESVEGFSTL KKVNVFNINN NRYLNSFQSS LESVSDSLQF SSNGDNTTLA
210 220 230 240 250
FDNLVWANNI TLRDVNSISF GSLQTVNASL GFINNTLPSL NLTQLSKVGQ
260 270 280 290 300
SLSIVSNDEL SKAAFSNLTT VGGGFIIANN TQLKVIDGFN KVQTVGGAIE
310 320 330 340 350
VTGNFSTLDL SSLKSVRGGA NFDSSSSNFS CNALKKLQSN GAIQGDSFVC
360 370 380 390 400
KNGATSTSVK LSSTSTESSK SSATSSASSS GDASNAQANV SASASSSSSS
410 420
SKKSKGAAPE LVPATSFMGV VAAVGVALL
Length:429
Mass (Da):43,769
Last modified:April 29, 2008 - v3
Checksum:iF57D94E1E53600CF
GO

Sequence cautioni

The sequence CAA53935 differs from that shown. Reason: Frameshift at position 428.Curated
The sequence CAA53935 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA85022 differs from that shown. Reason: Frameshift at position 428.Curated
The sequence CAA85023 differs from that shown. Reason: Frameshift at position 428.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76294 Genomic DNA Translation: CAA53935.1 Sequence problems.
Z35947 Genomic DNA Translation: CAA85022.1 Frameshift.
Z35948 Genomic DNA Translation: CAA85023.1 Frameshift.
BK006936 Genomic DNA Translation: DAA07197.2
PIRiS70297
RefSeqiNP_009634.2, NM_001178426.2

Genome annotation databases

EnsemblFungiiYBR078W; YBR078W; YBR078W
GeneIDi852370
KEGGisce:YBR078W

Similar proteinsi

Entry informationi

Entry nameiECM33_YEAST
AccessioniPrimary (citable) accession number: P38248
Secondary accession number(s): D6VQ77, P89498
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: April 29, 2008
Last modified: March 28, 2018
This is version 154 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health