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Protein

Cell wall protein ECM33

Gene

ECM33

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for proper cell wall integrity and for the correct assembly of the mannoprotein outer layer of the cell wall. Important for apical bud growth.3 Publications

GO - Biological processi

  • fungal-type cell wall organization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-29046-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell wall protein ECM33
Alternative name(s):
Extracellular mutant protein 33
Gene namesi
Name:ECM33
Ordered Locus Names:YBR078W
ORF Names:YBR0727
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR078W.
SGDiS000000282. ECM33.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • extracellular region Source: UniProtKB-KW
  • fungal-type cell wall Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000003319120 – 406Cell wall protein ECM33Add BLAST387
PropeptideiPRO_0000033192407 – 429Removed in mature formSequence analysisAdd BLAST23

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi21N-linked (GlcNAc...)Sequence analysis1
Glycosylationi56N-linked (GlcNAc...)Sequence analysis1
Glycosylationi82N-linked (GlcNAc...)Sequence analysis1
Glycosylationi196N-linked (GlcNAc...)Sequence analysis1
Glycosylationi209N-linked (GlcNAc...)Sequence analysis1
Glycosylationi227N-linked (GlcNAc...)Sequence analysis1
Glycosylationi234N-linked (GlcNAc...)Sequence analysis1
Glycosylationi241N-linked (GlcNAc...)Sequence analysis1
Glycosylationi267N-linked (GlcNAc...)Sequence analysis1
Glycosylationi279N-linked (GlcNAc...)Sequence analysis1
Glycosylationi304N-linked (GlcNAc...)Sequence analysis1
Glycosylationi328N-linked (GlcNAc...)Sequence analysis1
Modified residuei339PhosphoserineCombined sources1
Glycosylationi389N-linked (GlcNAc...)Sequence analysis1
Lipidationi406GPI-anchor amidated glycineSequence analysis1

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.Curated
Extensively N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

MaxQBiP38248.
PRIDEiP38248.

PTM databases

iPTMnetiP38248.

Interactioni

Protein-protein interaction databases

BioGridi32780. 239 interactors.
DIPiDIP-6584N.
IntActiP38248. 2 interactors.
MINTiMINT-4805607.

Structurei

3D structure databases

ProteinModelPortaliP38248.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi311 – 404Ser-richAdd BLAST94

Sequence similaritiesi

Belongs to the SPS2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00530000065410.
HOGENOMiHOG000093252.
InParanoidiP38248.
OMAiGNNMEIN.
OrthoDBiEOG092C3DSS.

Family and domain databases

Gene3Di3.80.20.20. 1 hit.
InterProiIPR032675. L_dom-like.
IPR000494. Rcpt_L-dom.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38248-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFKNALTAT AILSASALAA NSTTSIPSSC SIGTSATATA QADLDKISGC
60 70 80 90 100
STIVGNLTIT GDLGSAALAS IQEIDGSLTI FNSSSLSSFS ADSIKKITGD
110 120 130 140 150
LNMQELIILT SASFGSLQEV DSINMVTLPA ISTFSTDLQN ANNIIVSDTT
160 170 180 190 200
LESVEGFSTL KKVNVFNINN NRYLNSFQSS LESVSDSLQF SSNGDNTTLA
210 220 230 240 250
FDNLVWANNI TLRDVNSISF GSLQTVNASL GFINNTLPSL NLTQLSKVGQ
260 270 280 290 300
SLSIVSNDEL SKAAFSNLTT VGGGFIIANN TQLKVIDGFN KVQTVGGAIE
310 320 330 340 350
VTGNFSTLDL SSLKSVRGGA NFDSSSSNFS CNALKKLQSN GAIQGDSFVC
360 370 380 390 400
KNGATSTSVK LSSTSTESSK SSATSSASSS GDASNAQANV SASASSSSSS
410 420
SKKSKGAAPE LVPATSFMGV VAAVGVALL
Length:429
Mass (Da):43,769
Last modified:April 29, 2008 - v3
Checksum:iF57D94E1E53600CF
GO

Sequence cautioni

The sequence CAA53935 differs from that shown. Reason: Frameshift at position 428.Curated
The sequence CAA53935 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA85022 differs from that shown. Reason: Frameshift at position 428.Curated
The sequence CAA85023 differs from that shown. Reason: Frameshift at position 428.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76294 Genomic DNA. Translation: CAA53935.1. Sequence problems.
Z35947 Genomic DNA. Translation: CAA85022.1. Frameshift.
Z35948 Genomic DNA. Translation: CAA85023.1. Frameshift.
BK006936 Genomic DNA. Translation: DAA07197.2.
PIRiS70297.
RefSeqiNP_009634.2. NM_001178426.2.

Genome annotation databases

EnsemblFungiiYBR078W; YBR078W; YBR078W.
GeneIDi852370.
KEGGisce:YBR078W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76294 Genomic DNA. Translation: CAA53935.1. Sequence problems.
Z35947 Genomic DNA. Translation: CAA85022.1. Frameshift.
Z35948 Genomic DNA. Translation: CAA85023.1. Frameshift.
BK006936 Genomic DNA. Translation: DAA07197.2.
PIRiS70297.
RefSeqiNP_009634.2. NM_001178426.2.

3D structure databases

ProteinModelPortaliP38248.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32780. 239 interactors.
DIPiDIP-6584N.
IntActiP38248. 2 interactors.
MINTiMINT-4805607.

PTM databases

iPTMnetiP38248.

Proteomic databases

MaxQBiP38248.
PRIDEiP38248.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR078W; YBR078W; YBR078W.
GeneIDi852370.
KEGGisce:YBR078W.

Organism-specific databases

EuPathDBiFungiDB:YBR078W.
SGDiS000000282. ECM33.

Phylogenomic databases

GeneTreeiENSGT00530000065410.
HOGENOMiHOG000093252.
InParanoidiP38248.
OMAiGNNMEIN.
OrthoDBiEOG092C3DSS.

Enzyme and pathway databases

BioCyciYEAST:G3O-29046-MONOMER.

Miscellaneous databases

PROiP38248.

Family and domain databases

Gene3Di3.80.20.20. 1 hit.
InterProiIPR032675. L_dom-like.
IPR000494. Rcpt_L-dom.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiECM33_YEAST
AccessioniPrimary (citable) accession number: P38248
Secondary accession number(s): D6VQ77, P89498
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: April 29, 2008
Last modified: November 2, 2016
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6500 wall-bound molecules/cell in log phase YPD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.