Reviewed, UniProtKB/Swiss-Prot P38242 (ALG14_YEAST)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine transferase subunit ALG14
Alternative name(s):
    Asparagine-linked glycosylation protein 14

Gene names

Name:	ALG14
Ordered Locus Names:	YBR070C
ORF Names:	YBR0711

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway. Anchors the catalytic subunit ALG13 to the ER. Ref.5 Ref.6
Subunit structure	Heterodimer with ALG13 to form a functional enzyme.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Ref.6 Ref.4
Miscellaneous	Present with 339 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the ALG14 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Nucleus
Domain	Transmembrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dolichol-linked oligosaccharide biosynthetic process Ref.5 Ref.6 Inferred from direct assay. Source: SGD
Cellular component	UDP-N-acetylglucosamine transferase complex Ref.6 Inferred from physical interaction. Source: SGD integral to endoplasmic reticulum membrane Ref.6 Inferred from direct assay. Source: SGD membrane fraction Inferred from direct assay. Source: SGD nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein anchor Ref.6 Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ALG13	P53178	1	EBI-21477,EBI-23770
CAX4	P53223	1	EBI-21477,EBI-23136

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 237	237	UDP-N-acetylglucosamine transferase subunit ALG14		PRO_0000123818
Regions
	Topological domain	1 – 7	7	Lumenal Potential
	Transmembrane	8 – 28	21	Potential
	Topological domain	29 – 237	209	Cytoplasmic Potential
Amino acid modifications
	Modified residue	126	1	Phosphothreonine Ref.8
	Modified residue	130	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P38242-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 53E2D17E18593156	FASTA	237	27,035
	10 20 30 40 50 60 MKTAYLASLV LIVSTAYVIR LIAILPFFHT QAGTEKDTKD GVNLLKIRKS SKKPLKIFVF 70 80 90 100 110 120 LGSGGHTGEM IRLLENYQDL LLGKSIVYLG YSDEASRQRF AHFIKKFGHC KVKYYEFMKA 130 140 150 160 170 180 REVKATLLQS VKTIIGTLVQ SFVHVVRIRF AMCGSPHLFL LNGPGTCCII SFWLKIMELL 190 200 210 220 230 LPLLGSSHIV YVESLARINT PSLTGKILYW VVDEFIVQWQ ELRDNYLPRS KWFGILV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 31 kb DNA fragment from the right arm of Saccharomyces cerevisiae chromosome II." van der Aart Q.J.M., Barthe C., Doignon F., Aigle M., Crouzet M., Steensma H.Y. Yeast 10:959-964(1994) [PubMed: 7985423] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Localization of proteins that are coordinately expressed with Cln2 during the cell cycle." Sundin B.A., Chiu C.-H., Riffle M., Davis T.N., Muller E.G.D. Yeast 21:793-800(2004) [PubMed: 15282802] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Two proteins homologous to the N- and C-terminal domains of the bacterial glycosyltransferase Murg are required for the second step of dolichyl-linked oligosaccharide synthesis in Saccharomyces cerevisiae." Chantret I., Dancourt J., Barbat A., Moore S.E.H. J. Biol. Chem. 280:9236-9242(2005) [PubMed: 15615718] [Abstract] Cited for: FUNCTION.
[6]	"Alg14 recruits Alg13 to the cytoplasmic face of the endoplasmic reticulum to form a novel bipartite UDP-N-acetylglucosamine transferase required for the second step of N-linked glycosylation." Gao X.-D., Tachikawa H., Sato T., Jigami Y., Dean N. J. Biol. Chem. 280:36254-36262(2005) [PubMed: 16100110] [Abstract] Cited for: FUNCTION, INTERACTION WITH ALG13, SUBCELLULAR LOCATION.
[7]	"Membrane topology of the Alg14 endoplasmic reticulum UDP-GlcNAc transferase subunit." Averbeck N., Keppler-Ross S., Dean N. J. Biol. Chem. 282:29081-29088(2007) [PubMed: 17686769] [Abstract] Cited for: TOPOLOGY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-126 AND SER-130, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X76294 Genomic DNA. Translation: CAA53927.1. Z35939 Genomic DNA. Translation: CAA85014.1.
PIR	S45463.
RefSeq	NP_009626.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5670N.
IntAct	P38242. 6 interactions.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Genome annotation databases
Ensembl	YBR070C. Saccharomyces cerevisiae. [Contig view]
GeneID	852362.
GenomeReviews	Gene locus YBR070C in contig Y13134_GR.
KEGG	sce:YBR070C.
NMPDR	fig|4932.3.peg.323.
Organism-specific databases
CYGD	YBR070c.
SGD	S000000274. ALG14.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P38242.
OMA	P38242. NGPGTCC.
Enzyme and pathway databases
BRENDA	2.4.1.141. 250.
Gene expression databases
ArrayExpress	P38242.
GermOnline	YBR070C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013969. Oligosacch_biosynth_Alg14. [Graphical view]
PANTHER	PTHR12154. Oligosacch_biosynth_Alg14. 1 hit.
Pfam	PF08660. Alg14. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	971131.

Entry information

Entry name

ALG14_YEAST

Accession

Primary (citable) accession number: P38242

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)