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P38242

- ALG14_YEAST

UniProt

P38242 - ALG14_YEAST

Protein

UDP-N-acetylglucosamine transferase subunit ALG14

Gene

ALG14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway. Anchors the catalytic subunit ALG13 to the ER.2 Publications

    GO - Molecular functioni

    1. protein anchor Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. dolichol-linked oligosaccharide biosynthetic process Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29039-MONOMER.
    YEAST:MONOMER3O-23.
    BRENDAi2.4.1.141. 984.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-N-acetylglucosamine transferase subunit ALG14
    Alternative name(s):
    Asparagine-linked glycosylation protein 14
    Gene namesi
    Name:ALG14
    Ordered Locus Names:YBR070C
    ORF Names:YBR0711
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR070c.
    SGDiS000000274. ALG14.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of endoplasmic reticulum membrane Source: SGD
    2. intrinsic component of endoplasmic reticulum membrane Source: SGD
    3. nuclear membrane Source: UniProtKB-SubCell
    4. nuclear outer membrane-endoplasmic reticulum membrane network Source: SGD
    5. UDP-N-acetylglucosamine transferase complex Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 237237UDP-N-acetylglucosamine transferase subunit ALG14PRO_0000123818Add
    BLAST

    Proteomic databases

    MaxQBiP38242.
    PaxDbiP38242.

    Expressioni

    Gene expression databases

    GenevestigatoriP38242.

    Interactioni

    Subunit structurei

    Heterodimer with ALG13 to form a functional enzyme.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ALG13P531783EBI-21477,EBI-23770

    Protein-protein interaction databases

    BioGridi32773. 21 interactions.
    DIPiDIP-5670N.
    IntActiP38242. 5 interactions.
    MINTiMINT-508519.
    STRINGi4932.YBR070C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38242.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 77LumenalSequence Analysis
    Topological domaini29 – 237209CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALG14 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG261064.
    GeneTreeiENSGT00390000002579.
    HOGENOMiHOG000182099.
    KOiK07441.
    OMAiWSISTIP.
    OrthoDBiEOG7XM38T.

    Family and domain databases

    InterProiIPR013969. Oligosacch_biosynth_Alg14.
    [Graphical view]
    PANTHERiPTHR12154. PTHR12154. 1 hit.
    PfamiPF08660. Alg14. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38242-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTAYLASLV LIVSTAYVIR LIAILPFFHT QAGTEKDTKD GVNLLKIRKS    50
    SKKPLKIFVF LGSGGHTGEM IRLLENYQDL LLGKSIVYLG YSDEASRQRF 100
    AHFIKKFGHC KVKYYEFMKA REVKATLLQS VKTIIGTLVQ SFVHVVRIRF 150
    AMCGSPHLFL LNGPGTCCII SFWLKIMELL LPLLGSSHIV YVESLARINT 200
    PSLTGKILYW VVDEFIVQWQ ELRDNYLPRS KWFGILV 237
    Length:237
    Mass (Da):27,035
    Last modified:October 1, 1994 - v1
    Checksum:i53E2D17E18593156
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76294 Genomic DNA. Translation: CAA53927.1.
    Z35939 Genomic DNA. Translation: CAA85014.1.
    BK006936 Genomic DNA. Translation: DAA07189.1.
    PIRiS45463.
    RefSeqiNP_009626.1. NM_001178418.1.

    Genome annotation databases

    EnsemblFungiiYBR070C; YBR070C; YBR070C.
    GeneIDi852362.
    KEGGisce:YBR070C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76294 Genomic DNA. Translation: CAA53927.1 .
    Z35939 Genomic DNA. Translation: CAA85014.1 .
    BK006936 Genomic DNA. Translation: DAA07189.1 .
    PIRi S45463.
    RefSeqi NP_009626.1. NM_001178418.1.

    3D structure databases

    ProteinModelPortali P38242.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32773. 21 interactions.
    DIPi DIP-5670N.
    IntActi P38242. 5 interactions.
    MINTi MINT-508519.
    STRINGi 4932.YBR070C.

    Protein family/group databases

    CAZyi GT1. Glycosyltransferase Family 1.

    Proteomic databases

    MaxQBi P38242.
    PaxDbi P38242.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR070C ; YBR070C ; YBR070C .
    GeneIDi 852362.
    KEGGi sce:YBR070C.

    Organism-specific databases

    CYGDi YBR070c.
    SGDi S000000274. ALG14.

    Phylogenomic databases

    eggNOGi NOG261064.
    GeneTreei ENSGT00390000002579.
    HOGENOMi HOG000182099.
    KOi K07441.
    OMAi WSISTIP.
    OrthoDBi EOG7XM38T.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29039-MONOMER.
    YEAST:MONOMER3O-23.
    BRENDAi 2.4.1.141. 984.

    Miscellaneous databases

    NextBioi 971131.
    PROi P38242.

    Gene expression databases

    Genevestigatori P38242.

    Family and domain databases

    InterProi IPR013969. Oligosacch_biosynth_Alg14.
    [Graphical view ]
    PANTHERi PTHR12154. PTHR12154. 1 hit.
    Pfami PF08660. Alg14. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 31 kb DNA fragment from the right arm of Saccharomyces cerevisiae chromosome II."
      van der Aart Q.J.M., Barthe C., Doignon F., Aigle M., Crouzet M., Steensma H.Y.
      Yeast 10:959-964(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Localization of proteins that are coordinately expressed with Cln2 during the cell cycle."
      Sundin B.A., Chiu C.-H., Riffle M., Davis T.N., Muller E.G.D.
      Yeast 21:793-800(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    6. "Two proteins homologous to the N- and C-terminal domains of the bacterial glycosyltransferase Murg are required for the second step of dolichyl-linked oligosaccharide synthesis in Saccharomyces cerevisiae."
      Chantret I., Dancourt J., Barbat A., Moore S.E.H.
      J. Biol. Chem. 280:9236-9242(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Alg14 recruits Alg13 to the cytoplasmic face of the endoplasmic reticulum to form a novel bipartite UDP-N-acetylglucosamine transferase required for the second step of N-linked glycosylation."
      Gao X.-D., Tachikawa H., Sato T., Jigami Y., Dean N.
      J. Biol. Chem. 280:36254-36262(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ALG13, SUBCELLULAR LOCATION.
    8. "Membrane topology of the Alg14 endoplasmic reticulum UDP-GlcNAc transferase subunit."
      Averbeck N., Keppler-Ross S., Dean N.
      J. Biol. Chem. 282:29081-29088(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY.

    Entry informationi

    Entry nameiALG14_YEAST
    AccessioniPrimary (citable) accession number: P38242
    Secondary accession number(s): D6VQ69
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 339 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3