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P38242

- ALG14_YEAST

UniProt

P38242 - ALG14_YEAST

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Protein

UDP-N-acetylglucosamine transferase subunit ALG14

Gene

ALG14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway. Anchors the catalytic subunit ALG13 to the ER.2 Publications

GO - Molecular functioni

  1. protein anchor Source: SGD

GO - Biological processi

  1. dolichol-linked oligosaccharide biosynthetic process Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29039-MONOMER.
YEAST:MONOMER3O-23.
BRENDAi2.4.1.141. 984.
ReactomeiREACT_250523. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine transferase subunit ALG14
Alternative name(s):
Asparagine-linked glycosylation protein 14
Gene namesi
Name:ALG14
Ordered Locus Names:YBR070C
ORF Names:YBR0711
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR070c.
SGDiS000000274. ALG14.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77LumenalSequence Analysis
Transmembranei8 – 2821HelicalSequence AnalysisAdd
BLAST
Topological domaini29 – 237209CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of endoplasmic reticulum membrane Source: SGD
  2. intrinsic component of endoplasmic reticulum membrane Source: SGD
  3. nuclear outer membrane-endoplasmic reticulum membrane network Source: SGD
  4. nucleus Source: UniProtKB-KW
  5. UDP-N-acetylglucosamine transferase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237UDP-N-acetylglucosamine transferase subunit ALG14PRO_0000123818Add
BLAST

Proteomic databases

MaxQBiP38242.
PaxDbiP38242.

Expressioni

Gene expression databases

GenevestigatoriP38242.

Interactioni

Subunit structurei

Heterodimer with ALG13 to form a functional enzyme.

Binary interactionsi

WithEntry#Exp.IntActNotes
ALG13P531783EBI-21477,EBI-23770

Protein-protein interaction databases

BioGridi32773. 21 interactions.
DIPiDIP-5670N.
IntActiP38242. 5 interactions.
MINTiMINT-508519.
STRINGi4932.YBR070C.

Structurei

3D structure databases

ProteinModelPortaliP38242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALG14 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG261064.
GeneTreeiENSGT00390000002579.
HOGENOMiHOG000182099.
InParanoidiP38242.
KOiK07441.
OMAiWSISTIP.
OrthoDBiEOG7XM38T.

Family and domain databases

InterProiIPR013969. Oligosacch_biosynth_Alg14.
[Graphical view]
PANTHERiPTHR12154. PTHR12154. 1 hit.
PfamiPF08660. Alg14. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38242-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTAYLASLV LIVSTAYVIR LIAILPFFHT QAGTEKDTKD GVNLLKIRKS
60 70 80 90 100
SKKPLKIFVF LGSGGHTGEM IRLLENYQDL LLGKSIVYLG YSDEASRQRF
110 120 130 140 150
AHFIKKFGHC KVKYYEFMKA REVKATLLQS VKTIIGTLVQ SFVHVVRIRF
160 170 180 190 200
AMCGSPHLFL LNGPGTCCII SFWLKIMELL LPLLGSSHIV YVESLARINT
210 220 230
PSLTGKILYW VVDEFIVQWQ ELRDNYLPRS KWFGILV
Length:237
Mass (Da):27,035
Last modified:October 1, 1994 - v1
Checksum:i53E2D17E18593156
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76294 Genomic DNA. Translation: CAA53927.1.
Z35939 Genomic DNA. Translation: CAA85014.1.
BK006936 Genomic DNA. Translation: DAA07189.1.
PIRiS45463.
RefSeqiNP_009626.1. NM_001178418.1.

Genome annotation databases

EnsemblFungiiYBR070C; YBR070C; YBR070C.
GeneIDi852362.
KEGGisce:YBR070C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76294 Genomic DNA. Translation: CAA53927.1 .
Z35939 Genomic DNA. Translation: CAA85014.1 .
BK006936 Genomic DNA. Translation: DAA07189.1 .
PIRi S45463.
RefSeqi NP_009626.1. NM_001178418.1.

3D structure databases

ProteinModelPortali P38242.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32773. 21 interactions.
DIPi DIP-5670N.
IntActi P38242. 5 interactions.
MINTi MINT-508519.
STRINGi 4932.YBR070C.

Protein family/group databases

CAZyi GT1. Glycosyltransferase Family 1.

Proteomic databases

MaxQBi P38242.
PaxDbi P38242.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR070C ; YBR070C ; YBR070C .
GeneIDi 852362.
KEGGi sce:YBR070C.

Organism-specific databases

CYGDi YBR070c.
SGDi S000000274. ALG14.

Phylogenomic databases

eggNOGi NOG261064.
GeneTreei ENSGT00390000002579.
HOGENOMi HOG000182099.
InParanoidi P38242.
KOi K07441.
OMAi WSISTIP.
OrthoDBi EOG7XM38T.

Enzyme and pathway databases

BioCyci YEAST:G3O-29039-MONOMER.
YEAST:MONOMER3O-23.
BRENDAi 2.4.1.141. 984.
Reactomei REACT_250523. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

NextBioi 971131.
PROi P38242.

Gene expression databases

Genevestigatori P38242.

Family and domain databases

InterProi IPR013969. Oligosacch_biosynth_Alg14.
[Graphical view ]
PANTHERi PTHR12154. PTHR12154. 1 hit.
Pfami PF08660. Alg14. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 31 kb DNA fragment from the right arm of Saccharomyces cerevisiae chromosome II."
    van der Aart Q.J.M., Barthe C., Doignon F., Aigle M., Crouzet M., Steensma H.Y.
    Yeast 10:959-964(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Localization of proteins that are coordinately expressed with Cln2 during the cell cycle."
    Sundin B.A., Chiu C.-H., Riffle M., Davis T.N., Muller E.G.D.
    Yeast 21:793-800(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "Two proteins homologous to the N- and C-terminal domains of the bacterial glycosyltransferase Murg are required for the second step of dolichyl-linked oligosaccharide synthesis in Saccharomyces cerevisiae."
    Chantret I., Dancourt J., Barbat A., Moore S.E.H.
    J. Biol. Chem. 280:9236-9242(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Alg14 recruits Alg13 to the cytoplasmic face of the endoplasmic reticulum to form a novel bipartite UDP-N-acetylglucosamine transferase required for the second step of N-linked glycosylation."
    Gao X.-D., Tachikawa H., Sato T., Jigami Y., Dean N.
    J. Biol. Chem. 280:36254-36262(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ALG13, SUBCELLULAR LOCATION.
  8. "Membrane topology of the Alg14 endoplasmic reticulum UDP-GlcNAc transferase subunit."
    Averbeck N., Keppler-Ross S., Dean N.
    J. Biol. Chem. 282:29081-29088(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.

Entry informationi

Entry nameiALG14_YEAST
AccessioniPrimary (citable) accession number: P38242
Secondary accession number(s): D6VQ69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 26, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 339 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3