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P38242 (ALG14_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-N-acetylglucosamine transferase subunit ALG14
Alternative name(s):
Asparagine-linked glycosylation protein 14
Gene names
Name:ALG14
Ordered Locus Names:YBR070C
ORF Names:YBR0711
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway. Anchors the catalytic subunit ALG13 to the ER. Ref.6 Ref.7

Subunit structure

Heterodimer with ALG13 to form a functional enzyme.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein Ref.5 Ref.7.

Miscellaneous

Present with 339 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ALG14 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ALG13P531783EBI-21477,EBI-23770

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 237237UDP-N-acetylglucosamine transferase subunit ALG14
PRO_0000123818

Regions

Topological domain1 – 77Lumenal Potential
Transmembrane8 – 2821Helical; Potential
Topological domain29 – 237209Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P38242 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 53E2D17E18593156

FASTA23727,035
        10         20         30         40         50         60 
MKTAYLASLV LIVSTAYVIR LIAILPFFHT QAGTEKDTKD GVNLLKIRKS SKKPLKIFVF 

        70         80         90        100        110        120 
LGSGGHTGEM IRLLENYQDL LLGKSIVYLG YSDEASRQRF AHFIKKFGHC KVKYYEFMKA 

       130        140        150        160        170        180 
REVKATLLQS VKTIIGTLVQ SFVHVVRIRF AMCGSPHLFL LNGPGTCCII SFWLKIMELL 

       190        200        210        220        230 
LPLLGSSHIV YVESLARINT PSLTGKILYW VVDEFIVQWQ ELRDNYLPRS KWFGILV 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 31 kb DNA fragment from the right arm of Saccharomyces cerevisiae chromosome II."
van der Aart Q.J.M., Barthe C., Doignon F., Aigle M., Crouzet M., Steensma H.Y.
Yeast 10:959-964(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Localization of proteins that are coordinately expressed with Cln2 during the cell cycle."
Sundin B.A., Chiu C.-H., Riffle M., Davis T.N., Muller E.G.D.
Yeast 21:793-800(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"Two proteins homologous to the N- and C-terminal domains of the bacterial glycosyltransferase Murg are required for the second step of dolichyl-linked oligosaccharide synthesis in Saccharomyces cerevisiae."
Chantret I., Dancourt J., Barbat A., Moore S.E.H.
J. Biol. Chem. 280:9236-9242(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Alg14 recruits Alg13 to the cytoplasmic face of the endoplasmic reticulum to form a novel bipartite UDP-N-acetylglucosamine transferase required for the second step of N-linked glycosylation."
Gao X.-D., Tachikawa H., Sato T., Jigami Y., Dean N.
J. Biol. Chem. 280:36254-36262(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ALG13, SUBCELLULAR LOCATION.
[8]"Membrane topology of the Alg14 endoplasmic reticulum UDP-GlcNAc transferase subunit."
Averbeck N., Keppler-Ross S., Dean N.
J. Biol. Chem. 282:29081-29088(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X76294 Genomic DNA. Translation: CAA53927.1.
Z35939 Genomic DNA. Translation: CAA85014.1.
BK006936 Genomic DNA. Translation: DAA07189.1.
PIRS45463.
RefSeqNP_009626.1. NM_001178418.1.

3D structure databases

ProteinModelPortalP38242.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32773. 21 interactions.
DIPDIP-5670N.
IntActP38242. 5 interactions.
MINTMINT-508519.
STRING4932.YBR070C.

Protein family/group databases

CAZyGT1. Glycosyltransferase Family 1.

Proteomic databases

PaxDbP38242.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR070C; YBR070C; YBR070C.
GeneID852362.
KEGGsce:YBR070C.

Organism-specific databases

CYGDYBR070c.
SGDS000000274. ALG14.

Phylogenomic databases

eggNOGNOG261064.
GeneTreeENSGT00390000002579.
HOGENOMHOG000182099.
KOK07441.
OMAHWPALAT.
OrthoDBEOG7XM38T.

Enzyme and pathway databases

BioCycYEAST:G3O-29039-MONOMER.
YEAST:MONOMER3O-23.
BRENDA2.4.1.141. 984.

Gene expression databases

GenevestigatorP38242.

Family and domain databases

InterProIPR013969. Oligosacch_biosynth_Alg14.
[Graphical view]
PANTHERPTHR12154. PTHR12154. 1 hit.
PfamPF08660. Alg14. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio971131.
PROP38242.

Entry information

Entry nameALG14_YEAST
AccessionPrimary (citable) accession number: P38242
Secondary accession number(s): D6VQ69
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families