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Protein

tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase

Gene

TRM7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of tRNA(Phe), tRNA(Trp) and tRNA(Leu(UAA)). Requires TRM732 for methylation of the cytidine at position 32 and RTT10/TRM734 for methylation of the nucleotides at position 34 in substrate tRNAs. Lack of either of these modifications in tRNA(Phe) reduces formation of wybutosine from 1-methylguanosine at position 37.2 Publications

Miscellaneous

Present with 4110 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + cytidine(32)/guanosine(34) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(32)/2'-O-methylguanosine(34) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei55S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei57S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei83S-adenosyl-L-methionineUniRule annotation1
Binding sitei99S-adenosyl-L-methionineUniRule annotation1
Binding sitei124S-adenosyl-L-methionineUniRule annotation1
Active sitei164Proton acceptorUniRule annotation1

GO - Molecular functioni

  • tRNA methyltransferase activity Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • tRNA methylation Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:YBR061C-MONOMER
YEAST:YBR061C-MONOMER
BRENDAi2.1.1.205 984

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase (EC:2.1.1.205UniRule annotation)
Alternative name(s):
2'-O-ribose RNA methyltransferase TRM7
tRNA methylase 7
Gene namesi
Name:TRM7UniRule annotation
Ordered Locus Names:YBR061C
ORF Names:YBR0527
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR061C
SGDiS000000265 TRM7

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Causes severe growth and translation defects, which is due to reduced decoding of Phe codons by hypomodified tRNA(Phe).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001555871 – 310tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferaseAdd BLAST310

Proteomic databases

MaxQBiP38238
PaxDbiP38238
PRIDEiP38238

Interactioni

Subunit structurei

Interacts with TRM732; for 2'-O-methylation of cytidine 32 in substrate tRNAs. Interacts with RTT10/TRM734; for 2'-O-methylation of position 34 in substrate tRNAs.1 Publication

Protein-protein interaction databases

BioGridi32765, 62 interactors
DIPiDIP-4899N
IntActiP38238, 20 interactors
MINTiP38238
STRINGi4932.YBR061C

Structurei

3D structure databases

ProteinModelPortaliP38238
SMRiP38238
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00730000111146
HOGENOMiHOG000162368
KOiK14864
OMAiFDIYKPP
OrthoDBiEOG092C4T6A

Family and domain databases

HAMAPiMF_01547 RNA_methyltr_E, 1 hit
MF_03162 RNA_methyltr_E_TRM7, 1 hit
InterProiView protein in InterPro
IPR028590 RNA_methyltr_E_Trm7
IPR015507 rRNA-MeTfrase_E
IPR002877 rRNA_MeTrfase_FtsJ_dom
IPR029063 SAM-dependent_MTases
PANTHERiPTHR10920:SF12 PTHR10920:SF12, 1 hit
PfamiView protein in Pfam
PF01728 FtsJ, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

P38238-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKSSKDKRD LYYRKAKEQG YRARSAFKLL QLNDQFHFLD DPNLKRVVDL
60 70 80 90 100
CAAPGSWSQV LSRKLFDESP SSDKEDRKIV SVDLQPMSPI PHVTTLQADI
110 120 130 140 150
THPKTLARIL KLFGNEKADF VCSDGAPDVT GLHDLDEYVQ QQLIMSALQL
160 170 180 190 200
TACILKKGGT FVAKIFRGRD IDMLYSQLGY LFDKIVCAKP RSSRGTSLEA
210 220 230 240 250
FIVCLGYNPP SNWTPKLDVN TSVDEFFQGC FLNKLCISDK LSHWNEEERN
260 270 280 290 300
IAEFMACGSL QSFDSDATYH DLPSSVAGTS SSLDPVQSPT NPPYKKALEL
310
KRSGKLTRSV
Length:310
Mass (Da):34,701
Last modified:October 1, 1994 - v1
Checksum:i3C7F2583F2012CCB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35930 Genomic DNA Translation: CAA85004.1
BK006936 Genomic DNA Translation: DAA07181.1
PIRiS45919
RefSeqiNP_009617.3, NM_001178409.3

Genome annotation databases

EnsemblFungiiYBR061C; YBR061C; YBR061C
GeneIDi852353
KEGGisce:YBR061C

Similar proteinsi

Entry informationi

Entry nameiTRM7_YEAST
AccessioniPrimary (citable) accession number: P38238
Secondary accession number(s): D6VQ61
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 23, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

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