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Protein

Ubiquitin carboxyl-terminal hydrolase 14

Gene

UBP14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a role in the negative regulation of gluconeogenesis. Required for proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase). Accelerates proteasomal breakdown of ubiquitinated proteins as it disassembles free ubiquitin chains that would compete with ubiquitinated proteins to bind to the proteasome.2 Publications

Miscellaneous

Present with 3040 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei332Nucleophile1
Active sitei737Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri190 – 262UBP-typePROSITE-ProRule annotationAdd BLAST73

GO - Molecular functioni

  • thiol-dependent ubiquitin-specific protease activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • negative regulation of gluconeogenesis Source: SGD
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
  • protein deubiquitination Source: InterPro

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29029-MONOMER
ReactomeiR-SCE-5689880 Ub-specific processing proteases
R-SCE-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes

Protein family/group databases

MEROPSiC19.083

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 14 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 14
Glucose-induced degradation protein 6
Ubiquitin thioesterase 14
Ubiquitin-specific-processing protease 14
Gene namesi
Name:UBP14
Synonyms:GID6
Ordered Locus Names:YBR058C
ORF Names:YBR0515
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR058C
SGDiS000000262 UBP14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi332C → A: Loss of enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805991 – 781Ubiquitin carboxyl-terminal hydrolase 14Add BLAST781

Proteomic databases

MaxQBiP38237
PaxDbiP38237
PRIDEiP38237

Interactioni

Protein-protein interaction databases

BioGridi32761, 215 interactors
DIPiDIP-4897N
IntActiP38237, 18 interactors
MINTiP38237
STRINGi4932.YBR058C

Structurei

3D structure databases

ProteinModelPortaliP38237
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini323 – 781USPAdd BLAST459
Domaini576 – 626UBA 1PROSITE-ProRule annotationAdd BLAST51
Domaini649 – 689UBA 2PROSITE-ProRule annotationAdd BLAST41

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi92 – 95Poly-Asn4

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri190 – 262UBP-typePROSITE-ProRule annotationAdd BLAST73

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000000874
HOGENOMiHOG000162311
InParanoidiP38237
KOiK11836
OMAiMKEEHKP
OrthoDBiEOG092C26OU

Family and domain databases

CDDicd14298 UBA2_scUBP14_like, 1 hit
Gene3Di3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR001394 Peptidase_C19_UCH
IPR015940 UBA
IPR009060 UBA-like_sf
IPR033864 UBA2_scUBP14-like
IPR016652 Ubiquitinyl_hydrolase
IPR018200 USP_CS
IPR028889 USP_dom
IPR013083 Znf_RING/FYVE/PHD
IPR001607 Znf_UBP
PfamiView protein in Pfam
PF00627 UBA, 1 hit
PF00443 UCH, 1 hit
PF02148 zf-UBP, 1 hit
PIRSFiPIRSF016308 UBP, 1 hit
SMARTiView protein in SMART
SM00165 UBA, 2 hits
SM00290 ZnF_UBP, 1 hit
SUPFAMiSSF46934 SSF46934, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 2 hits
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit
PS50271 ZF_UBP, 1 hit

Sequencei

Sequence statusi: Complete.

P38237-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEAVLENVN VPAVVSKDEC IYCFESPYNE PLALNASPKH SLNICLNCFQ
60 70 80 90 100
ATCNRHVPLH IRVTEYACDT IHSNYLTIAK VEKPKQENVE ENNNNKKIKL
110 120 130 140 150
QVIETSEDDT HNTIWSLQRF NGENVPRTVL SKSTDSDISS TALEKIEKIL
160 170 180 190 200
KAKSQDFEDK KNSWVLEIST CPHTENFQIP SKPENTVNLN QCSSCDLTQN
210 220 230 240 250
LWLCLHCGNI GCGREQIGID GHSHALDHYR SNNNHPLAIK LGSLSSSTYD
260 270 280 290 300
LYCYACDDET RFPDNVNLGS ALQIYGINIQ EKIADEKTLV QLQVEQNENW
310 320 330 340 350
QFRMVDSSGK EFEKLSASKN YGCGLINLGN SCYLNSVIQS LVNGGVPNWS
360 370 380 390 400
LDFLGSKFPL DVVYPDNNLK CQWIKLLNAM KCEPELYPNG IKPTTFKKCI
410 420 430 440 450
GQNHQEFSSN RQQDAMEFLT FLLDLLDKKF FSSSSSGIPN PNDLVRFMME
460 470 480 490 500
DRLQCNICGK VKYSYEPTEA IQIPLEENDE PQDMLERIKA YFEGQTIEFK
510 520 530 540 550
CANCKEKVTA NKKPGFKSLP QTLILNPIRI RLQNWIPVKT SNELSLPGLI
560 570 580 590 600
DRDDMLDVSS YLSQGFDPQT ENLLPDEDEN RSSFTPNQCS ISQLIEMGFT
610 620 630 640 650
QNASVRALFN TGNQDAESAM NWLFQHMDDP DLNDPFVPPP NVPKKDKREV
660 670 680 690 700
DEVSLTSMLS MGLNPNLCRK ALILNNGDVN RSVEWVFNNM DDDGTFPEPE
710 720 730 740 750
VPNEEQQQKK DLGYSTAKPY ALTAVICHKG NSVHSGHYVV FIRKLVADKW
760 770 780
KWVLYNDEKL VAADSIEDMK KNGYIYFYTR C
Length:781
Mass (Da):88,630
Last modified:October 31, 2006 - v2
Checksum:i52B421DCD4A0FCBA
GO

Sequence cautioni

The sequence CAA85001 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA86402 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35927 Genomic DNA Translation: CAA85001.1 Different initiation.
Z46260 Genomic DNA Translation: CAA86402.1 Different initiation.
BK006936 Genomic DNA Translation: DAA07177.1
PIRiS45916
RefSeqiNP_009614.2, NM_001178406.1

Genome annotation databases

EnsemblFungiiYBR058C; YBR058C; YBR058C
GeneIDi852349
KEGGisce:YBR058C

Similar proteinsi

Entry informationi

Entry nameiUBP14_YEAST
AccessioniPrimary (citable) accession number: P38237
Secondary accession number(s): D6VQ57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 31, 2006
Last modified: May 23, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

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