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Protein

Ubiquitin carboxyl-terminal hydrolase 14

Gene

UBP14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in the negative regulation of gluconeogenesis. Required for proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase). Accelerates proteasomal breakdown of ubiquitinated proteins as it disassembles free ubiquitin chains that would compete with ubiquitinated proteins to bind to the proteasome.2 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei332Nucleophile1
Active sitei737Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri190 – 262UBP-typePROSITE-ProRule annotationAdd BLAST73

GO - Molecular functioni

  • thiol-dependent ubiquitin-specific protease activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • negative regulation of gluconeogenesis Source: SGD
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
  • protein deubiquitination Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29029-MONOMER.
ReactomeiR-SCE-5689880. Ub-specific processing proteases.
R-SCE-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.

Protein family/group databases

MEROPSiC19.083.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 14 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 14
Glucose-induced degradation protein 6
Ubiquitin thioesterase 14
Ubiquitin-specific-processing protease 14
Gene namesi
Name:UBP14
Synonyms:GID6
Ordered Locus Names:YBR058C
ORF Names:YBR0515
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR058C.
SGDiS000000262. UBP14.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi332C → A: Loss of enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805991 – 781Ubiquitin carboxyl-terminal hydrolase 14Add BLAST781

Proteomic databases

MaxQBiP38237.
PRIDEiP38237.

Interactioni

Protein-protein interaction databases

BioGridi32761. 140 interactors.
DIPiDIP-4897N.
IntActiP38237. 11 interactors.
MINTiMINT-486523.

Structurei

3D structure databases

ProteinModelPortaliP38237.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini323 – 781USPAdd BLAST459
Domaini576 – 626UBA 1PROSITE-ProRule annotationAdd BLAST51
Domaini649 – 689UBA 2PROSITE-ProRule annotationAdd BLAST41

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi92 – 95Poly-Asn4

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 2 UBA domains.PROSITE-ProRule annotation
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri190 – 262UBP-typePROSITE-ProRule annotationAdd BLAST73

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000000874.
HOGENOMiHOG000162311.
InParanoidiP38237.
KOiK11836.
OMAiKLPYPDP.
OrthoDBiEOG092C26OU.

Family and domain databases

CDDicd14298. UBA2_scUBP14_like. 1 hit.
Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR015940. UBA.
IPR009060. UBA-like.
IPR033864. UBA2_scUBP14-like.
IPR016652. Ubiquitinyl_hydrolase.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PIRSFiPIRSF016308. UBP. 1 hit.
SMARTiSM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38237-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEAVLENVN VPAVVSKDEC IYCFESPYNE PLALNASPKH SLNICLNCFQ
60 70 80 90 100
ATCNRHVPLH IRVTEYACDT IHSNYLTIAK VEKPKQENVE ENNNNKKIKL
110 120 130 140 150
QVIETSEDDT HNTIWSLQRF NGENVPRTVL SKSTDSDISS TALEKIEKIL
160 170 180 190 200
KAKSQDFEDK KNSWVLEIST CPHTENFQIP SKPENTVNLN QCSSCDLTQN
210 220 230 240 250
LWLCLHCGNI GCGREQIGID GHSHALDHYR SNNNHPLAIK LGSLSSSTYD
260 270 280 290 300
LYCYACDDET RFPDNVNLGS ALQIYGINIQ EKIADEKTLV QLQVEQNENW
310 320 330 340 350
QFRMVDSSGK EFEKLSASKN YGCGLINLGN SCYLNSVIQS LVNGGVPNWS
360 370 380 390 400
LDFLGSKFPL DVVYPDNNLK CQWIKLLNAM KCEPELYPNG IKPTTFKKCI
410 420 430 440 450
GQNHQEFSSN RQQDAMEFLT FLLDLLDKKF FSSSSSGIPN PNDLVRFMME
460 470 480 490 500
DRLQCNICGK VKYSYEPTEA IQIPLEENDE PQDMLERIKA YFEGQTIEFK
510 520 530 540 550
CANCKEKVTA NKKPGFKSLP QTLILNPIRI RLQNWIPVKT SNELSLPGLI
560 570 580 590 600
DRDDMLDVSS YLSQGFDPQT ENLLPDEDEN RSSFTPNQCS ISQLIEMGFT
610 620 630 640 650
QNASVRALFN TGNQDAESAM NWLFQHMDDP DLNDPFVPPP NVPKKDKREV
660 670 680 690 700
DEVSLTSMLS MGLNPNLCRK ALILNNGDVN RSVEWVFNNM DDDGTFPEPE
710 720 730 740 750
VPNEEQQQKK DLGYSTAKPY ALTAVICHKG NSVHSGHYVV FIRKLVADKW
760 770 780
KWVLYNDEKL VAADSIEDMK KNGYIYFYTR C
Length:781
Mass (Da):88,630
Last modified:October 31, 2006 - v2
Checksum:i52B421DCD4A0FCBA
GO

Sequence cautioni

The sequence CAA85001 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA86402 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35927 Genomic DNA. Translation: CAA85001.1. Different initiation.
Z46260 Genomic DNA. Translation: CAA86402.1. Different initiation.
BK006936 Genomic DNA. Translation: DAA07177.1.
PIRiS45916.
RefSeqiNP_009614.2. NM_001178406.1.

Genome annotation databases

EnsemblFungiiYBR058C; YBR058C; YBR058C.
GeneIDi852349.
KEGGisce:YBR058C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35927 Genomic DNA. Translation: CAA85001.1. Different initiation.
Z46260 Genomic DNA. Translation: CAA86402.1. Different initiation.
BK006936 Genomic DNA. Translation: DAA07177.1.
PIRiS45916.
RefSeqiNP_009614.2. NM_001178406.1.

3D structure databases

ProteinModelPortaliP38237.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32761. 140 interactors.
DIPiDIP-4897N.
IntActiP38237. 11 interactors.
MINTiMINT-486523.

Protein family/group databases

MEROPSiC19.083.

Proteomic databases

MaxQBiP38237.
PRIDEiP38237.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR058C; YBR058C; YBR058C.
GeneIDi852349.
KEGGisce:YBR058C.

Organism-specific databases

EuPathDBiFungiDB:YBR058C.
SGDiS000000262. UBP14.

Phylogenomic databases

GeneTreeiENSGT00390000000874.
HOGENOMiHOG000162311.
InParanoidiP38237.
KOiK11836.
OMAiKLPYPDP.
OrthoDBiEOG092C26OU.

Enzyme and pathway databases

BioCyciYEAST:G3O-29029-MONOMER.
ReactomeiR-SCE-5689880. Ub-specific processing proteases.
R-SCE-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.

Miscellaneous databases

PROiP38237.

Family and domain databases

CDDicd14298. UBA2_scUBP14_like. 1 hit.
Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR015940. UBA.
IPR009060. UBA-like.
IPR033864. UBA2_scUBP14-like.
IPR016652. Ubiquitinyl_hydrolase.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PIRSFiPIRSF016308. UBP. 1 hit.
SMARTiSM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP14_YEAST
AccessioniPrimary (citable) accession number: P38237
Secondary accession number(s): D6VQ57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 31, 2006
Last modified: November 30, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3040 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.