P38228 (TCM62_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial chaperone TCM62

Gene names

Name:	TCM62
Ordered Locus Names:	YBR044C
ORF Names:	YBR0414

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Chaperone. Required for the assembly of succinate dehydrogenase subunits. Ensures mitochondrial gene expression at elevated temperatures and prevents heat-aggregation of the ribosomal subunit VAR1.
Subunit structure	Forms a high molecular mass protein complex of approximately 850 kDa.
Subcellular location	Mitochondrion inner membrane; Single-pass membrane protein; Matrix side Ref.1 Ref.6.
Sequence similarities	Belongs to the chaperonin (HSP60) family.
Sequence caution	The sequence CAA84986.1 differs from that shown. Reason: Frameshift at position 566.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular protein metabolic process Inferred from electronic annotation. Source: InterPro mitochondrial respiratory chain complex II assembly Inferred from mutant phenotype. Source: SGD response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from direct assay Ref.1. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW unfolded protein binding Inferred from mutant phenotype Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 16	16	Mitochondrion Potential
	Chain	17 – 572	556	Mitochondrial chaperone TCM62		PRO_0000063639
Regions
	Topological domain	17 – 471	455	Mitochondrial matrix Potential
	Transmembrane	472 – 488	17	Helical; Potential
	Topological domain	489 – 572	84	Mitochondrial intermembrane Potential

Sequences

	Sequence		Length	Mass (Da)
	P38228 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: 1AAE3CD22AD8D5BD	FASTA	572	64,252
	10 20 30 40 50 60 MLRNCLRKLG NHQTKCSVKT LHTPIYRTKN LQVLRDTLSG IKLLEKIITS SSYNKTLIYE 70 80 90 100 110 120 PKYKSKPQVV SSHDTMRLHN VMRELLDSLQ VDEATNTRLQ SNRPRKLGRV GLQLFMDCIQ 130 140 150 160 170 180 DNLTATSTSL TCSLLEHYFK YPEKEVTNGI KAGLRYIRDF LAKNKIIVKS QNDVDALVEQ 190 200 210 220 230 240 LTMSSSDSQS IKRVLKAINY ELFSDDIVRV INGNKTYDEV DVSKGWKYPA GILDSNEAYL 250 260 270 280 290 300 RSLELPTKKL VSIDKDMLVL MYDGTLRDAN KILPTITYAR KLRKSVLLIV NGDCTGDALT 310 320 330 340 350 360 SVTINNNRNK RENNESRIVV LKYSKKANND LAPQENLDFI KFLRLPCGYD SIYSPEYSPL 370 380 390 400 410 420 VPSKMCADKY YGSIESIKAT TGEAFLYNSI DAEAIPNKVP KSFLQNTVTL SIGGHNEIEI 430 440 450 460 470 480 DRRRNAIDNC LNNVLCHGLA KGFIPGYGIS LLKAIPGLNE LKANEPNFMT KVGINAVLSA 490 500 510 520 530 540 VILPSEVAFK NAYGYNYYEI NSLIAGAINE KSFPMAKFSP NSEPVNTVKD GNLEPWSKMD 550 560 570 SCLAGVETFI ELLTSCNTII TCVYKKPERH KA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Saccharomyces cerevisiae TCM62 gene encodes a chaperone necessary for the assembly of the mitochondrial succinate dehydrogenase (complex II)." Dibrov E., Fu S., Lemire B.D. J. Biol. Chem. 273:32042-32048(1998) [PubMed: 9822678] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, SUBCELLULAR LOCATION, TOPOLOGY. Strain: S288c / GRF88.
[2]	Lemire B.D. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 566.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 566. Strain: ATCC 204508 / S288c.
[5]	"The chaperonin-related protein Tcm62p ensures mitochondrial gene expression under heat stress." Klanner C., Neupert W., Langer T. FEBS Lett. 470:365-369(2000) [PubMed: 10745098] [Abstract] Cited for: CHARACTERIZATION.
[6]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF125155 Genomic DNA. Translation: AAD24466.1. Z35913 Genomic DNA. Translation: CAA84986.1. Frameshift. BK006936 Genomic DNA. Translation: DAA07164.2.
PIR	S45902.
RefSeq	NP_009600.2. NM_001178392.2.
3D structure databases
ProteinModelPortal	P38228.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2609N.
IntAct	P38228. 2 interactions.
MINT	MINT-423791.
STRING	P38228.
Proteomic databases
PeptideAtlas	P38228.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR044C; YBR044C; YBR044C.
GeneID	852332.
KEGG	sce:YBR044C.
Organism-specific databases
SGD	S000000248. TCM62.
Phylogenomic databases
eggNOG	fuNOG04523.
GeneTree	EFGT00050000002192.
HOGENOM	HBG204129.
OrthoDB	EOG48H0C5.
Gene expression databases
ArrayExpress	P38228.
Genevestigator	P38228.
GermOnline	YBR044C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002423. Cpn60/TCP-1. [Graphical view]
PANTHER	PTHR11353. Cpn60/TCP-1. 1 hit.
Pfam	PF00118. Cpn60_TCP1. 1 hit. [Graphical view]
PROSITE	PS00296. CHAPERONINS_CPN60. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCM62_YEAST

Accession

Primary (citable) accession number: P38228
Secondary accession number(s): D6VQ44, Q9Y793

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	August 31, 2004
Last modified:	January 25, 2012
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program