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P38227 (QDR3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Quinidine resistance protein 3
Alternative name(s):
Acids quinidine resistance protein 2
Gene names
Name:QDR3
Synonyms:AQR2
Ordered Locus Names:YBR043C
ORF Names:YBR0413
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length689 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Multidrug resistance transporter involved in resistance and adaptation to quinidine and to the herbicide barban (4-chloro-2-butynyl [3-chlorophenyl] carbamate). Ref.5

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.3 Ref.5.

Miscellaneous

Present with 279 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the major facilitator superfamily. CAR1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 689689Quinidine resistance protein 3
PRO_0000173437

Regions

Topological domain1 – 108108Extracellular Potential
Transmembrane109 – 13123Helical; Potential
Topological domain132 – 1398Cytoplasmic Potential
Transmembrane140 – 16324Helical; Potential
Topological domain164 – 17512Extracellular Potential
Transmembrane176 – 19318Helical; Potential
Topological domain194 – 23542Cytoplasmic Potential
Transmembrane236 – 25621Helical; Potential
Topological domain257 – 2659Extracellular Potential
Transmembrane266 – 28318Helical; Potential
Topological domain284 – 475192Cytoplasmic Potential
Transmembrane476 – 49318Helical; Potential
Topological domain494 – 51017Extracellular Potential
Transmembrane511 – 53222Helical; Potential
Topological domain533 – 55826Cytoplasmic Potential
Transmembrane559 – 57719Helical; Potential
Topological domain578 – 5869Extracellular Potential
Transmembrane587 – 60923Helical; Potential
Topological domain610 – 62415Cytoplasmic Potential
Transmembrane625 – 64218Helical; Potential
Topological domain643 – 6486Extracellular Potential
Transmembrane649 – 66820Helical; Potential
Topological domain669 – 68921Cytoplasmic Potential

Amino acid modifications

Modified residue571Phosphoserine Ref.7

Experimental info

Sequence conflict371S → T in CAA84985. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P38227 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: AA2C5D2D7175F176

FASTA68977,287
        10         20         30         40         50         60 
MQAQGSQSNV GSLRSNCSDN SLPNNHVMMH CDESSGSPHS EHNDYSYEKT NLESTASNSR 

        70         80         90        100        110        120 
EHRDNQLSRL KSEEYVVPKN QRRGLLPQLA IIPEFKDARD YPPMMKKMIV FLIAFSSMMG 

       130        140        150        160        170        180 
PMGTSIIFPA INSITTEFKT SVIMVNVSIG VYLLSLGVFP LWWSSLSELE GRRTTYITSF 

       190        200        210        220        230        240 
ALLFAFNIGS ALAPDINSFI ALRMLCGAAS ASVQSVGAGT VADLYISEDR GKNLSYYYLG 

       250        260        270        280        290        300 
PLLAPLLSPI FGSLLVNRWP WRSTQWFMVI LSGCNVILLT VLLPETLRKQ DSKGAIAQIL 

       310        320        330        340        350        360 
AERRIQVDNN ERGEIQEDYQ RGEDETDRIE NQVATLSTEK HNYVGEVRDQ DSLDLESHSS 

       370        380        390        400        410        420 
PNTYDGRAGE TQLQRIYTEA SRSLYEYQLD DSGIDATTAQ VTRIRSTDPK LARSIRENSL 

       430        440        450        460        470        480 
RKLQTNLEEQ VKKVLSSNGG EIAPKQVSAV RKVWDTFFVY FIKPLKSLHF LEYPPVALAI 

       490        500        510        520        530        540 
TFSAISFSTV YFVNMTVEYK YSRPPYNFKP LYIGLLYIPN SVTYFFASIY GGRWVDMLLK 

       550        560        570        580        590        600 
RYKEKYGILA PEARISWNVV TSVISFPIAL LIFGWCLDKK CHWVTPLIGT ALFGYAAMMT 

       610        620        630        640        650        660 
IGATLSYLVD SLPGKGATGV ALNNLIRQIL AATAVFVTTP MLNGMGTGWA FTMLAFIVLG 

       670        680 
ASSVLIILKK HGDYWRENYD LQKLYDKID

« Hide

References

« Hide 'large scale' references
[1]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 37.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"The yeast multidrug transporter Qdr3 (Ybr043c): localization and role as a determinant of resistance to quinidine, barban, cisplatin, and bleomycin."
Tenreiro S., Vargas R.C., Teixeira M.C., Magnani C., Sa-Correia I.
Biochem. Biophys. Res. Commun. 327:952-959(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z35912 Genomic DNA. Translation: CAA84985.1.
BK006936 Genomic DNA. Translation: DAA07163.2.
PIRS45901.
RefSeqNP_009599.2. NM_001178391.2.

3D structure databases

ProteinModelPortalP38227.
SMRP38227. Positions 120-286.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-797N.
IntActP38227. 1 interaction.
MINTMINT-486852.
STRING4932.YBR043C.

Protein family/group databases

TCDB2.A.1.2.43. major facilitator superfamily (MFS).

Proteomic databases

PaxDbP38227.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR043C; YBR043C; YBR043C.
GeneID852331.
KEGGsce:YBR043C.

Organism-specific databases

CYGDYBR043c.
SGDS000000247. QDR3.

Phylogenomic databases

eggNOGCOG0477.
GeneTreeENSGT00620000088227.
OMAGTSIMLP.
OrthoDBEOG43246P.

Gene expression databases

GenevestigatorP38227.
GermOnlineYBR043C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. MFS_gen_substrate_transporter. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio971047.

Entry information

Entry nameQDR3_YEAST
AccessionPrimary (citable) accession number: P38227
Secondary accession number(s): D6VQ43
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 27, 2011
Last modified: May 1, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents