P38225 (FAT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Very long-chain fatty acid transport protein

Gene names

Name:	FAT1
Ordered Locus Names:	YBR041W
ORF Names:	YBR0411

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	669 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in long-chain fatty acids uptake, and thus may play a pivotal role in regulating their accessibility prior to metabolic utilization. May play an important role in uptake of these hydrophobic compounds under conditions where fatty acid synthesis is compromised.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Miscellaneous	Present with 16900 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.
Sequence caution	The sequence CAA84983.1 differs from that shown. Reason: Frameshift at position 620.

Ontologies

Keywords
Biological process	Lipid transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	very long chain fatty acid metabolic process Inferred from mutant phenotype. Source: SGD
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to internal side of plasma membrane Inferred from direct assay. Source: SGD lipid particle Inferred from direct assay. Source: SGD peroxisome Inferred from mutant phenotype. Source: SGD
Molecular function	long-chain fatty acid transporter activity Inferred from mutant phenotype Ref.1. Source: SGD very long-chain fatty acid-CoA ligase activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 669	669	Very long-chain fatty acid transport protein		PRO_0000193181
Regions
	Transmembrane	6 – 26	21	Helical; Potential
	Transmembrane	54 – 71	18	Helical; Potential
	Transmembrane	149 – 169	21	Helical; Potential
	Transmembrane	293 – 313	21	Helical; Potential
Amino acid modifications
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
	Glycosylation	289	1	N-linked (GlcNAc...) Potential
	Glycosylation	534	1	N-linked (GlcNAc...) Potential
	Glycosylation	591	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P38225 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: 509A5DEDDEE98823	FASTA	669	77,141
	10 20 30 40 50 60 MSPIQVVVFA LSRIFLLLFR LIKLIITPIQ KSLGYLFGNY FDELDRKYRY KEDWYIIPYF 70 80 90 100 110 120 LKSVFCYIID VRRHRFQNWY LFIKQVQQNG DHLAISYTRP MAEKGEFQLE TFTYIETYNI 130 140 150 160 170 180 VLRLSHILHF DYNVQAGDYV AIDCTNKPLF VFLWLSLWNI GAIPAFLNYN TKGTPLVHSL 190 200 210 220 230 240 KISNITQVFI DPDASNPIRE SEEEIKNALP DVKLNYLEEQ DLMHELLNSQ SPEFLQQDNV 250 260 270 280 290 300 RTPLGLTDFK PSMLIYTSGT TGLPKSAIMS WRKSSVGCQV FGHVLHMTNE STVFTAMPLF 310 320 330 340 350 360 HSTAALLGAC AILSHGGCLA LSHKFSASTF WKQVYLTGAT HIQYVGEVCR YLLHTPISKY 370 380 390 400 410 420 EKMHKVKVAY GNGLRPDIWQ DFRKRFNIEV IGEFYAATEA PFATTTFQKG DFGIGACRNY 430 440 450 460 470 480 GTIIQWFLSF QQTLVRMDPN DDSVIYRNSK GFCEVAPVGE PGEMLMRIFF PKKPETSFQG 490 500 510 520 530 540 YLGNAKETKS KVVRDVFRRG DAWYRCGDLL KADEYGLWYF LDRMGDTFRW KSENVSTTEV 550 560 570 580 590 600 EDQLTASNKE QYAQVLVVGI KVPKYEGRAG FAVIKLTDNS LDITAKTKLL NDSLSRLNLP 610 620 630 640 650 660 SYAMPLFVKF VDEIKMTDNH KILKKVYREQ KLPKGLDGND TIFWLKNYKR YEVLTAADWE AIDAQTIKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Disruption of the Saccharomyces cerevisiae homologue to the murine fatty acid transport protein impairs uptake and growth on long-chain fatty acids." Faergeman N.J., Dirusso C.C., Elberger A., Knudsen J., Black P.N. J. Biol. Chem. 272:8531-8538(1997) [PubMed: 9079682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: W303A.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Saccharomyces cerevisiae YBR041W sequence update." Zhang Z., Stuart L.T., Dietrich F.S. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 590-639. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF065148 Genomic DNA. Translation: AAC17118.1. Z35910 Genomic DNA. Translation: CAA84983.1. Frameshift. AY260890 Genomic DNA. Translation: AAP21758.1. BK006936 Genomic DNA. Translation: DAA07161.1.
PIR	S45899.
RefSeq	NP_009597.2. NM_001178389.1.
3D structure databases
ProteinModelPortal	P38225.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5048N.
IntAct	P38225. 19 interactions.
MINT	MINT-514313.
STRING	P38225.
Protein family/group databases
TCDB	4.C.1.1.2. proposed fatty acid transporter (FAT) family.
Proteomic databases
PeptideAtlas	P38225.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR041W; YBR041W; YBR041W.
GeneID	852329.
KEGG	sce:YBR041W.
NMPDR	fig|4932.3.peg.292.
Organism-specific databases
CYGD	YBR041w.
SGD	S000000245. FAT1.
Phylogenomic databases
eggNOG	fuNOG04525.
GeneTree	EFGT00050000001197.
HOGENOM	HBG712034.
OMA	WINSNLK.
OrthoDB	EOG4DJP4X.
Enzyme and pathway databases
BRENDA	6.2.1.3. 984.
Gene expression databases
ArrayExpress	P38225.
Genevestigator	P38225.
GermOnline	YBR041W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971041.

Entry information

Entry name

FAT1_YEAST

Accession

Primary (citable) accession number: P38225
Secondary accession number(s): D6VQ41, O60021, Q86ZS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	August 31, 2004
Last modified:	December 14, 2011
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program