Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P38221

- CDS1_YEAST

UniProt

P38221 - CDS1_YEAST

Protein

Phosphatidate cytidylyltransferase

Gene

CDS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Supplies CDP-diacylglycerol, which may play an important role as both a precursor to phosphoinositide biosynthesis in the plasma membrane and as a negative effector of phosphatidylinositol 4-kinase activity, thereby exerting an effect on cell proliferation via a lipid-dependent signal transduction cascade.2 Publications

    Catalytic activityi

    CTP + phosphatidate = diphosphate + CDP-diacylglycerol.1 Publication

    Cofactori

    Magnesium.1 Publication

    Kineticsi

    1. KM=1 mM for CTP1 Publication
    2. KM=0.5 mM for phosphatidate1 Publication

    Vmax=4700 nmol/min/mg enzyme1 Publication

    pH dependencei

    Optimum pH is 6.5.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. phosphatidate cytidylyltransferase activity Source: SGD

    GO - Biological processi

    1. CDP-diacylglycerol biosynthetic process Source: SGD
    2. phosphatidylinositol metabolic process Source: SGD
    3. phosphatidylserine metabolic process Source: SGD

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciYEAST:YBR029C-MONOMER.
    ReactomeiREACT_188995. Synthesis of PG.
    REACT_189002. Synthesis of PI.
    UniPathwayiUPA00557; UER00614.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidate cytidylyltransferase (EC:2.7.7.41)
    Alternative name(s):
    CDP-diacylglycerol synthase
    Short name:
    CDP-DAG synthase
    Short name:
    CDS
    CDP-diglyceride pyrophosphorylase
    CDP-diglyceride synthase
    Short name:
    CDP-DG synthase
    CTP:phosphatidate cytidylyltransferase
    Gene namesi
    Name:CDS1
    Synonyms:CDG1
    Ordered Locus Names:YBR029C
    ORF Names:YBR0313
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR029c.
    SGDiS000000233. CDS1.

    Subcellular locationi

    Endoplasmic reticulum membrane; Multi-pass membrane protein. Cytoplasmic vesiclesecretory vesicle
    Note: Exclusively present in the ER, and not in mitochondria. Also associated with post-Golgi apparatus secretory vesicles destined for the plasma membrane.

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. Golgi to plasma membrane transport vesicle Source: SGD
    4. integral component of membrane Source: UniProtKB-KW
    5. mitochondrial membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi102 – 1021C → Y: Reduced enzyme level. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 457457Phosphatidate cytidylyltransferasePRO_0000090722Add
    BLAST

    Proteomic databases

    MaxQBiP38221.
    PaxDbiP38221.
    PeptideAtlasiP38221.

    Expressioni

    Gene expression databases

    GenevestigatoriP38221.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi32731. 27 interactions.
    DIPiDIP-7898N.
    IntActiP38221. 3 interactions.
    MINTiMINT-1360573.
    STRINGi4932.YBR029C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38221.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei71 – 9121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei154 – 17421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei188 – 20821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei214 – 23421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei255 – 27521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei330 – 35021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CDS family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0575.
    GeneTreeiENSGT00390000016175.
    HOGENOMiHOG000209582.
    KOiK00981.
    OMAiVKCFQEI.
    OrthoDBiEOG7XDBQH.

    Family and domain databases

    InterProiIPR000374. PC_trans.
    IPR016720. PC_Trfase_euk.
    [Graphical view]
    PfamiPF01148. CTP_transf_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018269. PC_trans_euk. 1 hit.
    PROSITEiPS01315. CDS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38221-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDNPEMKPH GTSKEIVESV TDATSKAIDK LQEELHKDAS ESVTPVTKES    50
    TAATKESRKY NFFIRTVWTF VMISGFFITL ASGHAWCIVL ILGCQIATFK 100
    ECIAVTSASG REKNLPLTKT LNWYLLFTTI YYLDGKSLFK FFQATFYEYP 150
    VLNFIVTNHK FICYCLYLMG FVLFVCSLRK GFLKFQFGSL CVTHMVLLLV 200
    VFQAHLIIKN VLNGLFWFLL PCGLVIVNDI FAYLCGITFG KTKLIEISPK 250
    KTLEGFLGAW FFTALASIIL TRILSPYTYL TCPVEDLHTN FFSNLTCELN 300
    PVFLPQVYRL PPIFFDKVQI NSITVKPIYF HALNLATFAS LFAPFGGFFA 350
    SGLKRTFKVK DFGHSIPGHG GITDRVDCQF IMGSFANLYY ETFISEHRIT 400
    VDTVLSTILM NLNDKQIIEL IDILIRFLSK KGIISAKNFE KLADIFNVTK 450
    KSLTNHS 457
    Length:457
    Mass (Da):51,823
    Last modified:October 1, 1994 - v1
    Checksum:iF9F3613856EDE172
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76078 Genomic DNA. Translation: CAA53685.1.
    Z35898 Genomic DNA. Translation: CAA84971.1.
    AY693074 Genomic DNA. Translation: AAT93093.1.
    BK006936 Genomic DNA. Translation: DAA07150.1.
    PIRiS45885.
    RefSeqiNP_009585.1. NM_001178377.1.

    Genome annotation databases

    EnsemblFungiiYBR029C; YBR029C; YBR029C.
    GeneIDi852317.
    KEGGisce:YBR029C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76078 Genomic DNA. Translation: CAA53685.1 .
    Z35898 Genomic DNA. Translation: CAA84971.1 .
    AY693074 Genomic DNA. Translation: AAT93093.1 .
    BK006936 Genomic DNA. Translation: DAA07150.1 .
    PIRi S45885.
    RefSeqi NP_009585.1. NM_001178377.1.

    3D structure databases

    ProteinModelPortali P38221.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32731. 27 interactions.
    DIPi DIP-7898N.
    IntActi P38221. 3 interactions.
    MINTi MINT-1360573.
    STRINGi 4932.YBR029C.

    Proteomic databases

    MaxQBi P38221.
    PaxDbi P38221.
    PeptideAtlasi P38221.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR029C ; YBR029C ; YBR029C .
    GeneIDi 852317.
    KEGGi sce:YBR029C.

    Organism-specific databases

    CYGDi YBR029c.
    SGDi S000000233. CDS1.

    Phylogenomic databases

    eggNOGi COG0575.
    GeneTreei ENSGT00390000016175.
    HOGENOMi HOG000209582.
    KOi K00981.
    OMAi VKCFQEI.
    OrthoDBi EOG7XDBQH.

    Enzyme and pathway databases

    UniPathwayi UPA00557 ; UER00614 .
    BioCyci YEAST:YBR029C-MONOMER.
    Reactomei REACT_188995. Synthesis of PG.
    REACT_189002. Synthesis of PI.

    Miscellaneous databases

    NextBioi 971008.
    PROi P38221.

    Gene expression databases

    Genevestigatori P38221.

    Family and domain databases

    InterProi IPR000374. PC_trans.
    IPR016720. PC_Trfase_euk.
    [Graphical view ]
    Pfami PF01148. CTP_transf_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018269. PC_trans_euk. 1 hit.
    PROSITEi PS01315. CDS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The CDS1 gene encoding CDP-diacylglycerol synthase in Saccharomyces cerevisiae is essential for cell growth."
      Shen H., Heacock P.N., Clancey C.J., Dowhan W.
      J. Biol. Chem. 271:789-795(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    2. "The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
      Smits P.H.M., de Haan M., Maat C., Grivell L.A.
      Yeast 10:S75-S80(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Purification and characterization of CDP-diacylglycerol synthase from Saccharomyces cerevisiae."
      Kelley M.J., Carman G.M.
      J. Biol. Chem. 262:14563-14570(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: ATCC 204508 / S288c.
    7. "Enzymes of phosphoinositide synthesis in secretory vesicles destined for the plasma membrane in Saccharomyces cerevisiae."
      Kinney A.J., Carman G.M.
      J. Bacteriol. 172:4115-4117(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. "Reduction of CDP-diacylglycerol synthase activity results in the excretion of inositol by Saccharomyces cerevisiae."
      Shen H., Dowhan W.
      J. Biol. Chem. 271:29043-29048(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF CYS-102.
    9. "Tam41 is a CDP-diacylglycerol synthase required for cardiolipin biosynthesis in mitochondria."
      Tamura Y., Harada Y., Nishikawa S., Yamano K., Kamiya M., Shiota T., Kuroda T., Kuge O., Sesaki H., Imai K., Tomii K., Endo T.
      Cell Metab. 17:709-718(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCDS1_YEAST
    AccessioniPrimary (citable) accession number: P38221
    Secondary accession number(s): D6VQ30
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3