Reviewed, UniProtKB/Swiss-Prot P38221 (CDS1_YEAST)

Names and origin

Protein names

Recommended name:
    Phosphatidate cytidylyltransferase
    EC=2.7.7.41
Alternative name(s):
    CDP-diglyceride pyrophosphorylase
    CDP-diglyceride synthetase
    CDP-diacylglycerol synthase
      Short name=CDS
    CTP:phosphatidate cytidylyltransferase
    CDP-DG synthetase
    CDP-DAG synthase

Gene names

Name:	CDS1
Synonyms:	CDG1
Ordered Locus Names:	YBR029C
ORF Names:	YBR0313

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Supplies CDP-diacylglycerol, which may play an important role as both a precursor to phosphoinositide biosynthesis in the plasma membrane and as a negative effector of phosphatidylinositol 4-kinase activity, thereby exerting an effect on cell proliferation via a lipid-dependent signal transduction cascade.
Catalytic activity	CTP + phosphatidate = diphosphate + CDP-diacylglycerol.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.
Subunit structure	Homodimer.
Subcellular location	Mitochondrion membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the CDS family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion
Domain	Transmembrane
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	phosphatidate cytidylyltransferase activity Ref.1 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Phosphatidate cytidylyltransferase		PRO_0000090722
Regions
	Transmembrane	71 – 91	21	Potential
	Transmembrane	154 – 174	21	Potential
	Transmembrane	188 – 208	21	Potential
	Transmembrane	214 – 234	21	Potential
	Transmembrane	255 – 275	21	Potential
	Transmembrane	330 – 350	21	Potential
Experimental info
	Mutagenesis	102	1	C → Y: Reduced enzyme level. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P38221-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: F9F3613856EDE172	FASTA	457	51,823
	10 20 30 40 50 60 MSDNPEMKPH GTSKEIVESV TDATSKAIDK LQEELHKDAS ESVTPVTKES TAATKESRKY 70 80 90 100 110 120 NFFIRTVWTF VMISGFFITL ASGHAWCIVL ILGCQIATFK ECIAVTSASG REKNLPLTKT 130 140 150 160 170 180 LNWYLLFTTI YYLDGKSLFK FFQATFYEYP VLNFIVTNHK FICYCLYLMG FVLFVCSLRK 190 200 210 220 230 240 GFLKFQFGSL CVTHMVLLLV VFQAHLIIKN VLNGLFWFLL PCGLVIVNDI FAYLCGITFG 250 260 270 280 290 300 KTKLIEISPK KTLEGFLGAW FFTALASIIL TRILSPYTYL TCPVEDLHTN FFSNLTCELN 310 320 330 340 350 360 PVFLPQVYRL PPIFFDKVQI NSITVKPIYF HALNLATFAS LFAPFGGFFA SGLKRTFKVK 370 380 390 400 410 420 DFGHSIPGHG GITDRVDCQF IMGSFANLYY ETFISEHRIT VDTVLSTILM NLNDKQIIEL 430 440 450 IDILIRFLSK KGIISAKNFE KLADIFNVTK KSLTNHS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The CDS1 gene encoding CDP-diacylglycerol synthase in Saccharomyces cerevisiae is essential for cell growth." Shen H., Heacock P.N., Clancey C.J., Dowhan W. J. Biol. Chem. 271:789-795(1996) [PubMed: 8557688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]	"The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene." Smits P.H.M., de Haan M., Maat C., Grivell L.A. Yeast 10:S75-S80(1994) [PubMed: 8091864] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Reduction of CDP-diacylglycerol synthase activity results in the excretion of inositol by Saccharomyces cerevisiae." Shen H., Dowhan W. J. Biol. Chem. 271:29043-29048(1996) [PubMed: 8910557] [Abstract] Cited for: MUTAGENESIS OF CYS-102.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X76078 Genomic DNA. Translation: CAA53685.1. Z35898 Genomic DNA. Translation: CAA84971.1. AY693074 Genomic DNA. Translation: AAT93093.1.
PIR	S45885.
RefSeq	NP_009585.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:7898N.
IntAct	P38221. 2 interactions.
STRING	P38221.
Proteomic databases
PeptideAtlas	P38221.
Genome annotation databases
Ensembl	YBR029C; YBR029C; YBR029C; Saccharomyces cerevisiae. [Genome view]
GeneID	852317.
GenomeReviews	Gene locus YBR029C in contig Y13134_GR.
KEGG	sce:YBR029C.
NMPDR	fig|4932.3.peg.280.
Organism-specific databases
CYGD	YBR029c.
SGD	S000000233. CDS1.
Phylogenomic databases
HOGENOM	P38221.
OMA	IEISPKK.
Enzyme and pathway databases
BRENDA	2.7.7.41. 250.
Gene expression databases
ArrayExpress	P38221.
Genevestigator	P38221.
GermOnline	YBR029C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000374. PC_trans. IPR016720. PC_Trfase_euk. [Graphical view]
Pfam	PF01148. CTP_transf_1. 1 hit. [Graphical view]
PIRSF	PIRSF018269. PC_trans_euk. 1 hit.
PROSITE	PS01315. CDS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	971008.

Entry information

Entry name

CDS1_YEAST

Accession

Primary (citable) accession number: P38221

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 3, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)