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P38221

- CDS1_YEAST

UniProt

P38221 - CDS1_YEAST

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Protein

Phosphatidate cytidylyltransferase

Gene

CDS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Supplies CDP-diacylglycerol, which may play an important role as both a precursor to phosphoinositide biosynthesis in the plasma membrane and as a negative effector of phosphatidylinositol 4-kinase activity, thereby exerting an effect on cell proliferation via a lipid-dependent signal transduction cascade.2 Publications

Catalytic activityi

CTP + phosphatidate = diphosphate + CDP-diacylglycerol.1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

  1. KM=1 mM for CTP1 Publication
  2. KM=0.5 mM for phosphatidate1 Publication

Vmax=4700 nmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 6.5.1 Publication

Pathwayi

GO - Molecular functioni

  1. phosphatidate cytidylyltransferase activity Source: SGD

GO - Biological processi

  1. CDP-diacylglycerol biosynthetic process Source: SGD
  2. phosphatidylinositol metabolic process Source: SGD
  3. phosphatidylserine metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciYEAST:YBR029C-MONOMER.
ReactomeiREACT_188995. Synthesis of PG.
REACT_189002. Synthesis of PI.
UniPathwayiUPA00557; UER00614.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidate cytidylyltransferase (EC:2.7.7.41)
Alternative name(s):
CDP-diacylglycerol synthase
Short name:
CDP-DAG synthase
Short name:
CDS
CDP-diglyceride pyrophosphorylase
CDP-diglyceride synthase
Short name:
CDP-DG synthase
CTP:phosphatidate cytidylyltransferase
Gene namesi
Name:CDS1
Synonyms:CDG1
Ordered Locus Names:YBR029C
ORF Names:YBR0313
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR029c.
SGDiS000000233. CDS1.

Subcellular locationi

Endoplasmic reticulum membrane; Multi-pass membrane protein. Cytoplasmic vesiclesecretory vesicle
Note: Exclusively present in the ER, and not in mitochondria. Also associated with post-Golgi apparatus secretory vesicles destined for the plasma membrane.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei71 – 9121HelicalSequence AnalysisAdd
BLAST
Transmembranei154 – 17421HelicalSequence AnalysisAdd
BLAST
Transmembranei188 – 20821HelicalSequence AnalysisAdd
BLAST
Transmembranei214 – 23421HelicalSequence AnalysisAdd
BLAST
Transmembranei255 – 27521HelicalSequence AnalysisAdd
BLAST
Transmembranei330 – 35021HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. Golgi to plasma membrane transport vesicle Source: SGD
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi102 – 1021C → Y: Reduced enzyme level. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 457457Phosphatidate cytidylyltransferasePRO_0000090722Add
BLAST

Proteomic databases

MaxQBiP38221.
PaxDbiP38221.
PeptideAtlasiP38221.

Expressioni

Gene expression databases

GenevestigatoriP38221.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi32731. 27 interactions.
DIPiDIP-7898N.
IntActiP38221. 3 interactions.
MINTiMINT-1360573.
STRINGi4932.YBR029C.

Structurei

3D structure databases

ProteinModelPortaliP38221.
SMRiP38221. Positions 227-377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CDS family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0575.
GeneTreeiENSGT00390000016175.
HOGENOMiHOG000209582.
InParanoidiP38221.
KOiK00981.
OMAiVKCFQEI.
OrthoDBiEOG7XDBQH.

Family and domain databases

InterProiIPR000374. PC_trans.
IPR016720. PC_Trfase_euk.
[Graphical view]
PfamiPF01148. CTP_transf_1. 1 hit.
[Graphical view]
PIRSFiPIRSF018269. PC_trans_euk. 1 hit.
PROSITEiPS01315. CDS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38221-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDNPEMKPH GTSKEIVESV TDATSKAIDK LQEELHKDAS ESVTPVTKES
60 70 80 90 100
TAATKESRKY NFFIRTVWTF VMISGFFITL ASGHAWCIVL ILGCQIATFK
110 120 130 140 150
ECIAVTSASG REKNLPLTKT LNWYLLFTTI YYLDGKSLFK FFQATFYEYP
160 170 180 190 200
VLNFIVTNHK FICYCLYLMG FVLFVCSLRK GFLKFQFGSL CVTHMVLLLV
210 220 230 240 250
VFQAHLIIKN VLNGLFWFLL PCGLVIVNDI FAYLCGITFG KTKLIEISPK
260 270 280 290 300
KTLEGFLGAW FFTALASIIL TRILSPYTYL TCPVEDLHTN FFSNLTCELN
310 320 330 340 350
PVFLPQVYRL PPIFFDKVQI NSITVKPIYF HALNLATFAS LFAPFGGFFA
360 370 380 390 400
SGLKRTFKVK DFGHSIPGHG GITDRVDCQF IMGSFANLYY ETFISEHRIT
410 420 430 440 450
VDTVLSTILM NLNDKQIIEL IDILIRFLSK KGIISAKNFE KLADIFNVTK

KSLTNHS
Length:457
Mass (Da):51,823
Last modified:October 1, 1994 - v1
Checksum:iF9F3613856EDE172
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76078 Genomic DNA. Translation: CAA53685.1.
Z35898 Genomic DNA. Translation: CAA84971.1.
AY693074 Genomic DNA. Translation: AAT93093.1.
BK006936 Genomic DNA. Translation: DAA07150.1.
PIRiS45885.
RefSeqiNP_009585.1. NM_001178377.1.

Genome annotation databases

EnsemblFungiiYBR029C; YBR029C; YBR029C.
GeneIDi852317.
KEGGisce:YBR029C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76078 Genomic DNA. Translation: CAA53685.1 .
Z35898 Genomic DNA. Translation: CAA84971.1 .
AY693074 Genomic DNA. Translation: AAT93093.1 .
BK006936 Genomic DNA. Translation: DAA07150.1 .
PIRi S45885.
RefSeqi NP_009585.1. NM_001178377.1.

3D structure databases

ProteinModelPortali P38221.
SMRi P38221. Positions 227-377.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32731. 27 interactions.
DIPi DIP-7898N.
IntActi P38221. 3 interactions.
MINTi MINT-1360573.
STRINGi 4932.YBR029C.

Proteomic databases

MaxQBi P38221.
PaxDbi P38221.
PeptideAtlasi P38221.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR029C ; YBR029C ; YBR029C .
GeneIDi 852317.
KEGGi sce:YBR029C.

Organism-specific databases

CYGDi YBR029c.
SGDi S000000233. CDS1.

Phylogenomic databases

eggNOGi COG0575.
GeneTreei ENSGT00390000016175.
HOGENOMi HOG000209582.
InParanoidi P38221.
KOi K00981.
OMAi VKCFQEI.
OrthoDBi EOG7XDBQH.

Enzyme and pathway databases

UniPathwayi UPA00557 ; UER00614 .
BioCyci YEAST:YBR029C-MONOMER.
Reactomei REACT_188995. Synthesis of PG.
REACT_189002. Synthesis of PI.

Miscellaneous databases

NextBioi 971008.
PROi P38221.

Gene expression databases

Genevestigatori P38221.

Family and domain databases

InterProi IPR000374. PC_trans.
IPR016720. PC_Trfase_euk.
[Graphical view ]
Pfami PF01148. CTP_transf_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF018269. PC_trans_euk. 1 hit.
PROSITEi PS01315. CDS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The CDS1 gene encoding CDP-diacylglycerol synthase in Saccharomyces cerevisiae is essential for cell growth."
    Shen H., Heacock P.N., Clancey C.J., Dowhan W.
    J. Biol. Chem. 271:789-795(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
    Smits P.H.M., de Haan M., Maat C., Grivell L.A.
    Yeast 10:S75-S80(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Purification and characterization of CDP-diacylglycerol synthase from Saccharomyces cerevisiae."
    Kelley M.J., Carman G.M.
    J. Biol. Chem. 262:14563-14570(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: ATCC 204508 / S288c.
  7. "Enzymes of phosphoinositide synthesis in secretory vesicles destined for the plasma membrane in Saccharomyces cerevisiae."
    Kinney A.J., Carman G.M.
    J. Bacteriol. 172:4115-4117(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Reduction of CDP-diacylglycerol synthase activity results in the excretion of inositol by Saccharomyces cerevisiae."
    Shen H., Dowhan W.
    J. Biol. Chem. 271:29043-29048(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF CYS-102.
  9. "Tam41 is a CDP-diacylglycerol synthase required for cardiolipin biosynthesis in mitochondria."
    Tamura Y., Harada Y., Nishikawa S., Yamano K., Kamiya M., Shiota T., Kuroda T., Kuge O., Sesaki H., Imai K., Tomii K., Endo T.
    Cell Metab. 17:709-718(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCDS1_YEAST
AccessioniPrimary (citable) accession number: P38221
Secondary accession number(s): D6VQ30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 26, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3