Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphatidate cytidylyltransferase

Gene

CDS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Supplies CDP-diacylglycerol, which may play an important role as both a precursor to phosphoinositide biosynthesis in the plasma membrane and as a negative effector of phosphatidylinositol 4-kinase activity, thereby exerting an effect on cell proliferation via a lipid-dependent signal transduction cascade.2 Publications

Catalytic activityi

CTP + phosphatidate = diphosphate + CDP-diacylglycerol.1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

  1. KM=1 mM for CTP1 Publication
  2. KM=0.5 mM for phosphatidate1 Publication
  1. Vmax=4700 nmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 6.5.1 Publication

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate O-acyltransferase 2 (GPT2), Glycerol-3-phosphate O-acyltransferase 1 (SCT1)
  2. Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase (SLC1)
  3. Phosphatidate cytidylyltransferase (CDS1), Phosphatidate cytidylyltransferase, mitochondrial (TAM41)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • phosphatidate cytidylyltransferase activity Source: SGD

GO - Biological processi

  • CDP-diacylglycerol biosynthetic process Source: SGD
  • phosphatidylglycerol biosynthetic process Source: GO_Central
  • phosphatidylinositol metabolic process Source: SGD
  • phosphatidylserine metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciYEAST:YBR029C-MONOMER.
BRENDAi2.7.7.41. 984.
ReactomeiR-SCE-1483148. Synthesis of PG.
R-SCE-1483226. Synthesis of PI.
UniPathwayiUPA00557; UER00614.

Chemistry databases

SwissLipidsiSLP:000000050.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidate cytidylyltransferase (EC:2.7.7.41)
Alternative name(s):
CDP-diacylglycerol synthase
Short name:
CDP-DAG synthase
Short name:
CDS
CDP-diglyceride pyrophosphorylase
CDP-diglyceride synthase
Short name:
CDP-DG synthase
CTP:phosphatidate cytidylyltransferase
Gene namesi
Name:CDS1
Synonyms:CDG1
Ordered Locus Names:YBR029C
ORF Names:YBR0313
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR029C.
SGDiS000000233. CDS1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei71 – 91HelicalSequence analysisAdd BLAST21
Transmembranei154 – 174HelicalSequence analysisAdd BLAST21
Transmembranei188 – 208HelicalSequence analysisAdd BLAST21
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
Transmembranei255 – 275HelicalSequence analysisAdd BLAST21
Transmembranei330 – 350HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: GO_Central
  • Golgi to plasma membrane transport vesicle Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi102C → Y: Reduced enzyme level. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000907221 – 457Phosphatidate cytidylyltransferaseAdd BLAST457

Proteomic databases

MaxQBiP38221.
PRIDEiP38221.

PTM databases

iPTMnetiP38221.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi32731. 28 interactors.
DIPiDIP-7898N.
IntActiP38221. 3 interactors.
MINTiMINT-1360573.

Structurei

3D structure databases

ProteinModelPortaliP38221.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CDS family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000016175.
HOGENOMiHOG000209582.
InParanoidiP38221.
KOiK00981.
OMAiITRSIWT.
OrthoDBiEOG092C3NVX.

Family and domain databases

InterProiIPR000374. PC_trans.
IPR016720. PC_Trfase_euk.
[Graphical view]
PIRSFiPIRSF018269. PC_trans_euk. 1 hit.
PROSITEiPS01315. CDS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38221-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDNPEMKPH GTSKEIVESV TDATSKAIDK LQEELHKDAS ESVTPVTKES
60 70 80 90 100
TAATKESRKY NFFIRTVWTF VMISGFFITL ASGHAWCIVL ILGCQIATFK
110 120 130 140 150
ECIAVTSASG REKNLPLTKT LNWYLLFTTI YYLDGKSLFK FFQATFYEYP
160 170 180 190 200
VLNFIVTNHK FICYCLYLMG FVLFVCSLRK GFLKFQFGSL CVTHMVLLLV
210 220 230 240 250
VFQAHLIIKN VLNGLFWFLL PCGLVIVNDI FAYLCGITFG KTKLIEISPK
260 270 280 290 300
KTLEGFLGAW FFTALASIIL TRILSPYTYL TCPVEDLHTN FFSNLTCELN
310 320 330 340 350
PVFLPQVYRL PPIFFDKVQI NSITVKPIYF HALNLATFAS LFAPFGGFFA
360 370 380 390 400
SGLKRTFKVK DFGHSIPGHG GITDRVDCQF IMGSFANLYY ETFISEHRIT
410 420 430 440 450
VDTVLSTILM NLNDKQIIEL IDILIRFLSK KGIISAKNFE KLADIFNVTK

KSLTNHS
Length:457
Mass (Da):51,823
Last modified:October 1, 1994 - v1
Checksum:iF9F3613856EDE172
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76078 Genomic DNA. Translation: CAA53685.1.
Z35898 Genomic DNA. Translation: CAA84971.1.
AY693074 Genomic DNA. Translation: AAT93093.1.
BK006936 Genomic DNA. Translation: DAA07150.1.
PIRiS45885.
RefSeqiNP_009585.1. NM_001178377.1.

Genome annotation databases

EnsemblFungiiYBR029C; YBR029C; YBR029C.
GeneIDi852317.
KEGGisce:YBR029C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76078 Genomic DNA. Translation: CAA53685.1.
Z35898 Genomic DNA. Translation: CAA84971.1.
AY693074 Genomic DNA. Translation: AAT93093.1.
BK006936 Genomic DNA. Translation: DAA07150.1.
PIRiS45885.
RefSeqiNP_009585.1. NM_001178377.1.

3D structure databases

ProteinModelPortaliP38221.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32731. 28 interactors.
DIPiDIP-7898N.
IntActiP38221. 3 interactors.
MINTiMINT-1360573.

Chemistry databases

SwissLipidsiSLP:000000050.

PTM databases

iPTMnetiP38221.

Proteomic databases

MaxQBiP38221.
PRIDEiP38221.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR029C; YBR029C; YBR029C.
GeneIDi852317.
KEGGisce:YBR029C.

Organism-specific databases

EuPathDBiFungiDB:YBR029C.
SGDiS000000233. CDS1.

Phylogenomic databases

GeneTreeiENSGT00390000016175.
HOGENOMiHOG000209582.
InParanoidiP38221.
KOiK00981.
OMAiITRSIWT.
OrthoDBiEOG092C3NVX.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00614.
BioCyciYEAST:YBR029C-MONOMER.
BRENDAi2.7.7.41. 984.
ReactomeiR-SCE-1483148. Synthesis of PG.
R-SCE-1483226. Synthesis of PI.

Miscellaneous databases

PROiP38221.

Family and domain databases

InterProiIPR000374. PC_trans.
IPR016720. PC_Trfase_euk.
[Graphical view]
PIRSFiPIRSF018269. PC_trans_euk. 1 hit.
PROSITEiPS01315. CDS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDS1_YEAST
AccessioniPrimary (citable) accession number: P38221
Secondary accession number(s): D6VQ30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.