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Protein

Importin subunit beta-2

Gene

KAP104

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for arginine/glycine-rich nuclear localization signals (rg-NLS) and PY-NLS in cargo substrates. Its predominant cargo substrate seems to be mRNA-binding proteins. Required for nuclear transport of NAB2, HRP1/NAB4 and TFG2. Mediates docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to repeat-containing nucleoporins (PubMed:8849456, PubMed:9488461, PubMed:10506153, PubMed:19366694). The complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:11423015). At the nucleoplasmic side of the NPC, GTP-Ran binding leads to release of the cargo. Efficient GTP-Ran-mediated substrate release requires RNA (PubMed:10506153). The importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11423015).1 Publication4 Publications

Miscellaneous

Binds to nucleoporin FxFG but not PSFG repeat regions.1 Publication
Present with 2130 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • nuclear localization sequence binding Source: SGD
  • protein transporter activity Source: GO_Central

GO - Biological processi

  • exit from mitosis Source: SGD
  • mRNA transport Source: UniProtKB-KW
  • NLS-bearing protein import into nucleus Source: GO_Central
  • protein import into nucleus Source: SGD
  • protein import into nucleus, translocation Source: GO_Central
  • ribosomal protein import into nucleus Source: GO_Central

Keywordsi

Biological processmRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29001-MONOMER
ReactomeiR-SCE-5620924 Intraflagellar transport

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-2By similarity
Alternative name(s):
Importin-104
Karyopherin subunit beta-2
Karyopherin-1041 Publication
TransportinBy similarity
Short name:
TRNBy similarity
Gene namesi
Name:KAP1041 Publication
Ordered Locus Names:YBR017CImported
ORF Names:YBR0224
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR017C
SGDiS000000221 KAP104

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001207701 – 918Importin subunit beta-2Add BLAST918

Proteomic databases

MaxQBiP38217
PaxDbiP38217
PRIDEiP38217

Interactioni

Subunit structurei

Interacts with Ran (GSP1) (PubMed:9321403, PubMed:10506153); interacts specifically with the GTP-bound form of Ran (GTP-Ran), protecting it from GTP hydrolysis and nucleotide exchange (PubMed:9321403). Interacts with nucleoporins NUP1, NUP100 and NUP116 (PubMed:8849456). Interacts with NAB2 and HRP1/NAB4; via their rg-NLS (PubMed:9488461, PubMed:10506153). Interacts with TFG2; via its PY-NLS (PubMed:19366694).5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32720, 236 interactors
DIPiDIP-1399N
IntActiP38217, 110 interactors
MINTiP38217
STRINGi4932.YBR017C

Structurei

3D structure databases

ProteinModelPortaliP38217
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati11 – 38HEAT 1By similarityAdd BLAST28
Repeati43 – 92HEAT 2By similarityAdd BLAST50
Repeati103 – 137HEAT 3By similarityAdd BLAST35
Repeati145 – 181HEAT 4By similarityAdd BLAST37
Repeati190 – 222HEAT 5By similarityAdd BLAST33
Repeati235 – 263HEAT 6By similarityAdd BLAST29
Repeati275 – 303HEAT 7By similarityAdd BLAST29
Repeati320 – 413HEAT 8By similarityAdd BLAST94
Repeati421 – 449HEAT 9By similarityAdd BLAST29
Repeati461 – 488HEAT 10By similarityAdd BLAST28
Repeati501 – 534HEAT 11By similarityAdd BLAST34
Repeati542 – 577HEAT 12By similarityAdd BLAST36
Repeati583 – 620HEAT 13By similarityAdd BLAST38
Repeati628 – 678HEAT 14By similarityAdd BLAST51
Repeati694 – 725HEAT 15By similarityAdd BLAST32
Repeati777 – 814HEAT 17By similarityAdd BLAST38
Repeati825 – 858HEAT 18By similarityAdd BLAST34
Repeati867 – 900HEAT 19By similarityAdd BLAST34

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi373 – 392Asp/Glu-rich (acidic)Add BLAST20

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074720
HOGENOMiHOG000203940
InParanoidiP38217
OMAiVRRHVCQ
OrthoDBiEOG092C0UPN

Family and domain databases

InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR000357 HEAT
PfamiView protein in Pfam
PF02985 HEAT, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit

Sequencei

Sequence statusi: Complete.

P38217-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTWKPAED YVLQLATLLQ NCMSPNPEIR NNAMEAMENF QLQPEFLNYL
60 70 80 90 100
CYILIEGESD DVLKQHYSLQ DLQNNRATAG MLLKNSMLGG NNLIKSNSHD
110 120 130 140 150
LGYVKSNIIH GLYNSNNNLV SNVTGIVITT LFSTYYRQHR DDPTGLQMLY
160 170 180 190 200
QLLELTSNGN EPSIKALSKI MEDSAQFFQL EWSGNTKPME ALLDSFFRFI
210 220 230 240 250
SNPNFSPVIR SESVKCINTV IPLQTQSFIV RLDKFLEIIF QLAQNDENDL
260 270 280 290 300
VRAQICISFS FLLEFRPDKL VSHLDGIVQF MLHLITTVNE EKVAIEACEF
310 320 330 340 350
LHAFATSPNI PEHILQPYVK DIVPILLSKM VYNEESIVLL EASNDDDAFL
360 370 380 390 400
EDKDEDIKPI APRIVKKKEA GNGEDADDNE DDDDDDDDED GDVDTQWNLR
410 420 430 440 450
KCSAATLDVM TNILPHQVMD IAFPFLREHL GSDRWFIREA TILALGAMAE
460 470 480 490 500
GGMKYFNDGL PALIPFLVEQ LNDKWAPVRK MTCWTLSRFS PWILQDHTEF
510 520 530 540 550
LIPVLEPIIN TLMDKKKDVQ EAAISSVAVF IENADSELVE TLFYSQLLTS
560 570 580 590 600
FDKCLKYYKK KNLIILYDAI GRFAEKCALD ETAMQIILPP LIEKWALLSD
610 620 630 640 650
SDKELWPLLE CLSCVASSLG ERFMPMAPEV YNRAFRILCH CVELEAKSHQ
660 670 680 690 700
DPTIVVPEKD FIITSLDLID GLVQGLGAHS QDLLFPQGTK DLTILKIMLE
710 720 730 740 750
CLQDPVHEVR QSCFALLGDI VYFFNSELVI GNLEDFLKLI GTEIMHNDDS
760 770 780 790 800
DGTPAVINAI WALGLISERI DLNTYIIDMS RIILDLFTTN TQIVDSSVME
810 820 830 840 850
NLSVTIGKMG LTHPEVFSSG AFANDSNWNK WCLSVNALDD VEEKSSAYMG
860 870 880 890 900
FLKIINLTST EVTMSNDTIH KIVTGLSSNV EANVFAQEIY TFLMNHSAQI
910
SAINFTPDEI SFLQQFTS
Length:918
Mass (Da):103,681
Last modified:October 1, 1994 - v1
Checksum:iE8010A477D1FC0B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81324 Genomic DNA Translation: CAA57104.1
Z35886 Genomic DNA Translation: CAA84959.1
BK006936 Genomic DNA Translation: DAA07139.1
PIRiS45872
RefSeqiNP_009573.1, NM_001178365.1

Genome annotation databases

EnsemblFungiiYBR017C; YBR017C; YBR017C
GeneIDi852305
KEGGisce:YBR017C

Similar proteinsi

Entry informationi

Entry nameiIMB2_YEAST
AccessioniPrimary (citable) accession number: P38217
Secondary accession number(s): D6VQ19
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 23, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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