Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Importin subunit beta-2

Gene

KAP104

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for arginine/glycine-rich nuclear localization signals (rg-NLS) and PY-NLS in cargo substrates. Its predominant cargo substrate seems to be mRNA-binding proteins. Required for nuclear transport of NAB2, HRP1/NAB4 and TFG2. Mediates docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to repeat-containing nucleoporins (PubMed:8849456, PubMed:9488461, PubMed:10506153, PubMed:19366694). The complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:11423015). At the nucleoplasmic side of the NPC, GTP-Ran binding leads to release of the cargo. Efficient GTP-Ran-mediated substrate release requires RNA (PubMed:10506153). The importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11423015).1 Publication4 Publications

GO - Molecular functioni

  • nuclear localization sequence binding Source: SGD
  • protein transporter activity Source: GO_Central

GO - Biological processi

  • exit from mitosis Source: SGD
  • mRNA-binding (hnRNP) protein import into nucleus Source: SGD
  • mRNA transport Source: UniProtKB-KW
  • NLS-bearing protein import into nucleus Source: GO_Central
  • protein import into nucleus Source: SGD
  • protein import into nucleus, docking Source: GO_Central
  • protein import into nucleus, translocation Source: GO_Central
  • ribosomal protein import into nucleus Source: GO_Central
  • transcription factor import into nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29001-MONOMER.
ReactomeiR-SCE-5620924. Intraflagellar transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-2By similarity
Alternative name(s):
Importin-104
Karyopherin subunit beta-2
Karyopherin-1041 Publication
TransportinBy similarity
Short name:
TRNBy similarity
Gene namesi
Name:KAP1041 Publication
Ordered Locus Names:YBR017CImported
ORF Names:YBR0224
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR017C.
SGDiS000000221. KAP104.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleusnuclear pore complex 1 Publication
  • Nucleus 1 Publication

  • Note: Predominantly cytoplasmic.1 Publication

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • cytosol Source: SGD
  • nuclear membrane Source: GO_Central
  • nuclear periphery Source: GO_Central
  • nuclear pore Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001207701 – 918Importin subunit beta-2Add BLAST918

Proteomic databases

MaxQBiP38217.
PRIDEiP38217.

Interactioni

Subunit structurei

Interacts with Ran (GSP1) (PubMed:9321403, PubMed:10506153); interacts specifically with the GTP-bound form of Ran (GTP-Ran), protecting it from GTP hydrolysis and nucleotide exchange (PubMed:9321403). Interacts with nucleoporins NUP1, NUP100 and NUP116 (PubMed:8849456). Interacts with NAB2 and HRP1/NAB4; via their rg-NLS (PubMed:9488461, PubMed:10506153). Interacts with TFG2; via its PY-NLS (PubMed:19366694).5 Publications

Protein-protein interaction databases

BioGridi32720. 83 interactors.
DIPiDIP-1399N.
IntActiP38217. 33 interactors.
MINTiMINT-397734.

Structurei

3D structure databases

ProteinModelPortaliP38217.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati11 – 38HEAT 1By similarityAdd BLAST28
Repeati43 – 92HEAT 2By similarityAdd BLAST50
Repeati103 – 137HEAT 3By similarityAdd BLAST35
Repeati145 – 181HEAT 4By similarityAdd BLAST37
Repeati190 – 222HEAT 5By similarityAdd BLAST33
Repeati235 – 263HEAT 6By similarityAdd BLAST29
Repeati275 – 303HEAT 7By similarityAdd BLAST29
Repeati320 – 413HEAT 8By similarityAdd BLAST94
Repeati421 – 449HEAT 9By similarityAdd BLAST29
Repeati461 – 488HEAT 10By similarityAdd BLAST28
Repeati501 – 534HEAT 11By similarityAdd BLAST34
Repeati542 – 577HEAT 12By similarityAdd BLAST36
Repeati583 – 620HEAT 13By similarityAdd BLAST38
Repeati628 – 678HEAT 14By similarityAdd BLAST51
Repeati694 – 725HEAT 15By similarityAdd BLAST32
Repeati777 – 814HEAT 17By similarityAdd BLAST38
Repeati825 – 858HEAT 18By similarityAdd BLAST34
Repeati867 – 900HEAT 19By similarityAdd BLAST34

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi373 – 392Asp/Glu-rich (acidic)Add BLAST20

Sequence similaritiesi

Contains 19 HEAT repeats.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074720.
HOGENOMiHOG000203940.
InParanoidiP38217.
OMAiARYRDIN.
OrthoDBiEOG092C0UPN.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
[Graphical view]
PfamiPF02985. HEAT. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

P38217-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTWKPAED YVLQLATLLQ NCMSPNPEIR NNAMEAMENF QLQPEFLNYL
60 70 80 90 100
CYILIEGESD DVLKQHYSLQ DLQNNRATAG MLLKNSMLGG NNLIKSNSHD
110 120 130 140 150
LGYVKSNIIH GLYNSNNNLV SNVTGIVITT LFSTYYRQHR DDPTGLQMLY
160 170 180 190 200
QLLELTSNGN EPSIKALSKI MEDSAQFFQL EWSGNTKPME ALLDSFFRFI
210 220 230 240 250
SNPNFSPVIR SESVKCINTV IPLQTQSFIV RLDKFLEIIF QLAQNDENDL
260 270 280 290 300
VRAQICISFS FLLEFRPDKL VSHLDGIVQF MLHLITTVNE EKVAIEACEF
310 320 330 340 350
LHAFATSPNI PEHILQPYVK DIVPILLSKM VYNEESIVLL EASNDDDAFL
360 370 380 390 400
EDKDEDIKPI APRIVKKKEA GNGEDADDNE DDDDDDDDED GDVDTQWNLR
410 420 430 440 450
KCSAATLDVM TNILPHQVMD IAFPFLREHL GSDRWFIREA TILALGAMAE
460 470 480 490 500
GGMKYFNDGL PALIPFLVEQ LNDKWAPVRK MTCWTLSRFS PWILQDHTEF
510 520 530 540 550
LIPVLEPIIN TLMDKKKDVQ EAAISSVAVF IENADSELVE TLFYSQLLTS
560 570 580 590 600
FDKCLKYYKK KNLIILYDAI GRFAEKCALD ETAMQIILPP LIEKWALLSD
610 620 630 640 650
SDKELWPLLE CLSCVASSLG ERFMPMAPEV YNRAFRILCH CVELEAKSHQ
660 670 680 690 700
DPTIVVPEKD FIITSLDLID GLVQGLGAHS QDLLFPQGTK DLTILKIMLE
710 720 730 740 750
CLQDPVHEVR QSCFALLGDI VYFFNSELVI GNLEDFLKLI GTEIMHNDDS
760 770 780 790 800
DGTPAVINAI WALGLISERI DLNTYIIDMS RIILDLFTTN TQIVDSSVME
810 820 830 840 850
NLSVTIGKMG LTHPEVFSSG AFANDSNWNK WCLSVNALDD VEEKSSAYMG
860 870 880 890 900
FLKIINLTST EVTMSNDTIH KIVTGLSSNV EANVFAQEIY TFLMNHSAQI
910
SAINFTPDEI SFLQQFTS
Length:918
Mass (Da):103,681
Last modified:October 1, 1994 - v1
Checksum:iE8010A477D1FC0B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81324 Genomic DNA. Translation: CAA57104.1.
Z35886 Genomic DNA. Translation: CAA84959.1.
BK006936 Genomic DNA. Translation: DAA07139.1.
PIRiS45872.
RefSeqiNP_009573.1. NM_001178365.1.

Genome annotation databases

EnsemblFungiiYBR017C; YBR017C; YBR017C.
GeneIDi852305.
KEGGisce:YBR017C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81324 Genomic DNA. Translation: CAA57104.1.
Z35886 Genomic DNA. Translation: CAA84959.1.
BK006936 Genomic DNA. Translation: DAA07139.1.
PIRiS45872.
RefSeqiNP_009573.1. NM_001178365.1.

3D structure databases

ProteinModelPortaliP38217.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32720. 83 interactors.
DIPiDIP-1399N.
IntActiP38217. 33 interactors.
MINTiMINT-397734.

Proteomic databases

MaxQBiP38217.
PRIDEiP38217.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR017C; YBR017C; YBR017C.
GeneIDi852305.
KEGGisce:YBR017C.

Organism-specific databases

EuPathDBiFungiDB:YBR017C.
SGDiS000000221. KAP104.

Phylogenomic databases

GeneTreeiENSGT00550000074720.
HOGENOMiHOG000203940.
InParanoidiP38217.
OMAiARYRDIN.
OrthoDBiEOG092C0UPN.

Enzyme and pathway databases

BioCyciYEAST:G3O-29001-MONOMER.
ReactomeiR-SCE-5620924. Intraflagellar transport.

Miscellaneous databases

PROiP38217.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
[Graphical view]
PfamiPF02985. HEAT. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIMB2_YEAST
AccessioniPrimary (citable) accession number: P38217
Secondary accession number(s): D6VQ19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds to nucleoporin FxFG but not PSFG repeat regions.1 Publication
Present with 2130 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.