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Protein

Importin subunit beta-2

Gene

KAP104

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for arginine/glycine-rich nuclear localization signals (rg-NLS) and PY-NLS in cargo substrates. Its predominant cargo substrate seems to be mRNA-binding proteins. Required for nuclear transport of NAB2, HRP1/NAB4 and TFG2. Mediates docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to repeat-containing nucleoporins (PubMed:8849456, PubMed:9488461, PubMed:10506153, PubMed:19366694). The complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:11423015). At the nucleoplasmic side of the NPC, GTP-Ran binding leads to release of the cargo. Efficient GTP-Ran-mediated substrate release requires RNA (PubMed:10506153). The importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11423015).1 Publication4 Publications

GO - Molecular functioni

  • nuclear localization sequence binding Source: SGD

GO - Biological processi

  • exit from mitosis Source: SGD
  • mRNA-binding (hnRNP) protein import into nucleus Source: SGD
  • mRNA transport Source: UniProtKB-KW
  • protein import into nucleus Source: SGD
  • transcription factor import into nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29001-MONOMER.
ReactomeiR-SCE-5620924. Intraflagellar transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-2By similarity
Alternative name(s):
Importin-104
Karyopherin subunit beta-2
Karyopherin-1041 Publication
TransportinBy similarity
Short name:
TRNBy similarity
Gene namesi
Name:KAP1041 Publication
Ordered Locus Names:YBR017CImported
ORF Names:YBR0224
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR017C.
SGDiS000000221. KAP104.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleusnuclear pore complex 1 Publication
  • Nucleus 1 Publication

  • Note: Predominantly cytoplasmic.1 Publication

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • cytosol Source: SGD
  • nuclear pore Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 918918Importin subunit beta-2PRO_0000120770Add
BLAST

Proteomic databases

MaxQBiP38217.
PeptideAtlasiP38217.

Interactioni

Subunit structurei

Interacts with Ran (GSP1) (PubMed:9321403, PubMed:10506153); interacts specifically with the GTP-bound form of Ran (GTP-Ran), protecting it from GTP hydrolysis and nucleotide exchange (PubMed:9321403). Interacts with nucleoporins NUP1, NUP100 and NUP116 (PubMed:8849456). Interacts with NAB2 and HRP1/NAB4; via their rg-NLS (PubMed:9488461, PubMed:10506153). Interacts with TFG2; via its PY-NLS (PubMed:19366694).5 Publications

Protein-protein interaction databases

BioGridi32720. 83 interactions.
DIPiDIP-1399N.
IntActiP38217. 33 interactions.
MINTiMINT-397734.

Structurei

3D structure databases

ProteinModelPortaliP38217.
SMRiP38217. Positions 8-38, 143-169, 397-531, 694-766.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati11 – 3828HEAT 1By similarityAdd
BLAST
Repeati43 – 9250HEAT 2By similarityAdd
BLAST
Repeati103 – 13735HEAT 3By similarityAdd
BLAST
Repeati145 – 18137HEAT 4By similarityAdd
BLAST
Repeati190 – 22233HEAT 5By similarityAdd
BLAST
Repeati235 – 26329HEAT 6By similarityAdd
BLAST
Repeati275 – 30329HEAT 7By similarityAdd
BLAST
Repeati320 – 41394HEAT 8By similarityAdd
BLAST
Repeati421 – 44929HEAT 9By similarityAdd
BLAST
Repeati461 – 48828HEAT 10By similarityAdd
BLAST
Repeati501 – 53434HEAT 11By similarityAdd
BLAST
Repeati542 – 57736HEAT 12By similarityAdd
BLAST
Repeati583 – 62038HEAT 13By similarityAdd
BLAST
Repeati628 – 67851HEAT 14By similarityAdd
BLAST
Repeati694 – 72532HEAT 15By similarityAdd
BLAST
Repeati777 – 81438HEAT 17By similarityAdd
BLAST
Repeati825 – 85834HEAT 18By similarityAdd
BLAST
Repeati867 – 90034HEAT 19By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi373 – 39220Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 19 HEAT repeats.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074720.
HOGENOMiHOG000203940.
InParanoidiP38217.
OMAiAVPMCLQ.
OrthoDBiEOG7BW0T1.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
[Graphical view]
PfamiPF02985. HEAT. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

P38217-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTWKPAED YVLQLATLLQ NCMSPNPEIR NNAMEAMENF QLQPEFLNYL
60 70 80 90 100
CYILIEGESD DVLKQHYSLQ DLQNNRATAG MLLKNSMLGG NNLIKSNSHD
110 120 130 140 150
LGYVKSNIIH GLYNSNNNLV SNVTGIVITT LFSTYYRQHR DDPTGLQMLY
160 170 180 190 200
QLLELTSNGN EPSIKALSKI MEDSAQFFQL EWSGNTKPME ALLDSFFRFI
210 220 230 240 250
SNPNFSPVIR SESVKCINTV IPLQTQSFIV RLDKFLEIIF QLAQNDENDL
260 270 280 290 300
VRAQICISFS FLLEFRPDKL VSHLDGIVQF MLHLITTVNE EKVAIEACEF
310 320 330 340 350
LHAFATSPNI PEHILQPYVK DIVPILLSKM VYNEESIVLL EASNDDDAFL
360 370 380 390 400
EDKDEDIKPI APRIVKKKEA GNGEDADDNE DDDDDDDDED GDVDTQWNLR
410 420 430 440 450
KCSAATLDVM TNILPHQVMD IAFPFLREHL GSDRWFIREA TILALGAMAE
460 470 480 490 500
GGMKYFNDGL PALIPFLVEQ LNDKWAPVRK MTCWTLSRFS PWILQDHTEF
510 520 530 540 550
LIPVLEPIIN TLMDKKKDVQ EAAISSVAVF IENADSELVE TLFYSQLLTS
560 570 580 590 600
FDKCLKYYKK KNLIILYDAI GRFAEKCALD ETAMQIILPP LIEKWALLSD
610 620 630 640 650
SDKELWPLLE CLSCVASSLG ERFMPMAPEV YNRAFRILCH CVELEAKSHQ
660 670 680 690 700
DPTIVVPEKD FIITSLDLID GLVQGLGAHS QDLLFPQGTK DLTILKIMLE
710 720 730 740 750
CLQDPVHEVR QSCFALLGDI VYFFNSELVI GNLEDFLKLI GTEIMHNDDS
760 770 780 790 800
DGTPAVINAI WALGLISERI DLNTYIIDMS RIILDLFTTN TQIVDSSVME
810 820 830 840 850
NLSVTIGKMG LTHPEVFSSG AFANDSNWNK WCLSVNALDD VEEKSSAYMG
860 870 880 890 900
FLKIINLTST EVTMSNDTIH KIVTGLSSNV EANVFAQEIY TFLMNHSAQI
910
SAINFTPDEI SFLQQFTS
Length:918
Mass (Da):103,681
Last modified:October 1, 1994 - v1
Checksum:iE8010A477D1FC0B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81324 Genomic DNA. Translation: CAA57104.1.
Z35886 Genomic DNA. Translation: CAA84959.1.
BK006936 Genomic DNA. Translation: DAA07139.1.
PIRiS45872.
RefSeqiNP_009573.1. NM_001178365.1.

Genome annotation databases

EnsemblFungiiYBR017C; YBR017C; YBR017C.
GeneIDi852305.
KEGGisce:YBR017C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81324 Genomic DNA. Translation: CAA57104.1.
Z35886 Genomic DNA. Translation: CAA84959.1.
BK006936 Genomic DNA. Translation: DAA07139.1.
PIRiS45872.
RefSeqiNP_009573.1. NM_001178365.1.

3D structure databases

ProteinModelPortaliP38217.
SMRiP38217. Positions 8-38, 143-169, 397-531, 694-766.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32720. 83 interactions.
DIPiDIP-1399N.
IntActiP38217. 33 interactions.
MINTiMINT-397734.

Proteomic databases

MaxQBiP38217.
PeptideAtlasiP38217.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR017C; YBR017C; YBR017C.
GeneIDi852305.
KEGGisce:YBR017C.

Organism-specific databases

EuPathDBiFungiDB:YBR017C.
SGDiS000000221. KAP104.

Phylogenomic databases

GeneTreeiENSGT00550000074720.
HOGENOMiHOG000203940.
InParanoidiP38217.
OMAiAVPMCLQ.
OrthoDBiEOG7BW0T1.

Enzyme and pathway databases

BioCyciYEAST:G3O-29001-MONOMER.
ReactomeiR-SCE-5620924. Intraflagellar transport.

Miscellaneous databases

NextBioi970975.
PROiP38217.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
[Graphical view]
PfamiPF02985. HEAT. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and functional analysis of a 7.2 kb fragment of Saccharomyces cerevisiae chromosome II including GAL7 and GAL10 and a new essential open reading frame."
    Schaaff-Gerstenschlaeger I., Schindwolf T., Lehnert W., Rose M., Zimmermann F.K.
    Yeast 11:79-83(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins."
    Aitchison J.D., Blobel G., Rout M.P.
    Science 274:624-627(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH NAB2; HRP1; NUP1; NUP100 AND NUP106.
  5. "Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus."
    Schlenstedt G., Smirnova E., Deane R., Solsbacher J., Kutay U., Goerlich D., Ponstingl H., Bischoff F.R.
    EMBO J. 16:6237-6249(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GSP1.
  6. "Identification and functional characterization of a novel nuclear localization signal present in the yeast Nab2 poly(A)+ RNA binding protein."
    Truant R., Fridell R.A., Benson R.E., Bogerd H., Cullen B.R.
    Mol. Cell. Biol. 18:1449-1458(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NAB2.
  7. "Kap104p-mediated nuclear import. Nuclear localization signals in mRNA-binding proteins and the role of Ran and RNA."
    Lee D.C., Aitchison J.D.
    J. Biol. Chem. 274:29031-29037(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NAB2; HRP1 AND GSP1.
  8. "Importin-beta-like nuclear transport receptors."
    Stroem A.C., Weis K.
    Genome Biol. 2:REVIEWS3008.1-REVIEWS3008.9(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Kap104p imports the PY-NLS-containing transcription factor Tfg2p into the nucleus."
    Suel K.E., Chook Y.M.
    J. Biol. Chem. 284:15416-15424(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TFG2.

Entry informationi

Entry nameiIMB2_YEAST
AccessioniPrimary (citable) accession number: P38217
Secondary accession number(s): D6VQ19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 11, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds to nucleoporin FxFG but not PSFG repeat regions.1 Publication
Present with 2130 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.