P38211 (GPI18_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GPI mannosyltransferase 2
EC=2.4.1.-

Alternative name(s):
GPI mannosyltransferase II
Short name=GPI-MT-II
Glycosylphosphatidylinositol-anchor biosynthesis protein 18

Gene names

Name:	GPI18
Ordered Locus Names:	YBR004C
ORF Names:	YBR0110

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the second mannose to the glycosylphosphatidylinositol during GPI precursor assembly. Ref.3 Ref.4
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the PIGV family.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GPI anchor biosynthetic process Inferred from mutant phenotype. Source: SGD
Cellular component	integral to endoplasmic reticulum membrane Inferred from direct assay. Source: SGD mannosyltransferase complex Inferred from physical interaction. Source: SGD mitochondrion Inferred from direct assay. Source: SGD
Molecular function	dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase activity Inferred from mutant phenotype Ref.3. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ECM4	P36156	1	EBI-21377,EBI-2042717
KRE27	P40540	1	EBI-21377,EBI-24977
YHR080C	P38800	1	EBI-21377,EBI-24597

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	GPI mannosyltransferase 2		PRO_0000014309
Regions
	Transmembrane	2 – 22	21	Helical; Potential
	Transmembrane	110 – 130	21	Helical; Potential
	Transmembrane	151 – 168	18	Helical; Potential
	Transmembrane	170 – 182	13	Helical; Potential
	Transmembrane	198 – 220	23	Helical; Potential
	Transmembrane	222 – 236	15	Helical; Potential
	Transmembrane	244 – 264	21	Helical; Potential
	Transmembrane	319 – 339	21	Helical; Potential
	Transmembrane	351 – 371	21	Helical; Potential
	Transmembrane	410 – 430	21	Helical; Potential
Amino acid modifications
	Glycosylation	69	1	N-linked (GlcNAc...) Potential
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	187	1	S → T in CAA84940. Ref.1
	Sequence conflict	187	1	S → T in CAA84942. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P38211 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 9C81918DF1A37B44	FASTA	433	50,759
	10 20 30 40 50 60 MIVGLTLYFV LFRSIQYLLV FLTPIRQFDT STSLLLNELC SSPSEINSYW NKYFWNKLLS 70 80 90 100 110 120 WDSVFFIKNI TSKNGKPQFE HEYAFSQLWT FFVRLFIKSN NDSIYHALRV GVAIENVLFY 130 140 150 160 170 180 LSGIVLYFLT KKIFSQNIRQ SQFARTIAKK TSLLFFLTSA AGFLTSIYSE PLSFFFAFVG 190 200 210 220 230 240 IWSRECSISV PVLGQFDISW RYWFPYSFIS MACFTLASLN RSNCVLLGIY FIFDLIELTK 250 260 270 280 290 300 NRKFVKAICF PLLSGSLMFS ALLYQQYYLP YKTFCPQRGE WCKSQLFSSI FITKTSLYSY 310 320 330 340 350 360 IQSHYWGVGL LKYWTPNNIP NFLFAVPNII ILIYSSIYFS KIYPSYNLKA LVWITRALVV 370 380 390 400 410 420 IVCFFAHVQI LNRIASFLPL HLWYLADRLV KTSDPKKMEN PKGDDKIVKF YIYWLAFWIP 430 LQTILFAAFL PPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 187. Strain: ATCC 204508 / S288c.
[3]	"Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly." Fabre A.-L., Orlean P., Taron C.H. FEBS J. 272:1160-1168(2005) [PubMed: 15720390] [Abstract] Cited for: FUNCTION.
[4]	"PIG-V involved in transferring the second mannose in glycosylphosphatidylinositol." Kang J.Y., Hong Y., Ashida H., Shishioh N., Murakami Y., Morita Y.S., Maeda Y., Kinoshita T. J. Biol. Chem. 280:9489-9497(2005) [PubMed: 15623507] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z35873 Genomic DNA. Translation: CAA84940.1. Z35874 Genomic DNA. Translation: CAA84942.1. BK006936 Genomic DNA. Translation: DAA07125.2.
PIR	S45856.
RefSeq	NP_009558.2. NM_001178352.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3954N.
IntAct	P38211. 3 interactions.
MINT	MINT-478575.
STRING	P38211.
Protein family/group databases
CAZy	GT76. Glycosyltransferase Family 76.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	852289.
KEGG	sce:YBR004C.
NMPDR	fig|4932.3.peg.253.
Organism-specific databases
CYGD	YBR004c.
SGD	S000000208. GPI18.
Phylogenomic databases
eggNOG	fuNOG08000.
GeneTree	EFGT00050000006849.
HOGENOM	HBG396018.
OMA	NIPNFLF.
OrthoDB	EOG4PVS82.
Gene expression databases
ArrayExpress	P38211.
Genevestigator	P38211.
GermOnline	YBR004C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007315. GPI_Mannosyltransferase_2-like. [Graphical view]
KO	K07542.
PANTHER	PTHR12468. Mannosyl_trans2. 1 hit.
Pfam	PF04188. Mannosyl_trans2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970931.

Entry information

Entry name

GPI18_YEAST

Accession

Primary (citable) accession number: P38211
Secondary accession number(s): D6VQ05, P89496

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	July 27, 2011
Last modified:	January 25, 2012
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program