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P38207

- APN2_YEAST

UniProt

P38207 - APN2_YEAST

Protein

DNA-(apurinic or apyrimidinic site) lyase 2

Gene

APN2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    DNA repair enzyme that cleaves apurinic/apyrimidinic (AP) sites and removes 3'-blocking groups present at single strand breaks of damaged DNA.

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

    Cofactori

    Magnesium. Can also utilize manganese. Probably binds two magnesium or manganese ions per subunit By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi59 – 591Magnesium 1By similarity
    Active sitei181 – 1811By similarity
    Active sitei222 – 2221Proton donor/acceptorBy similarity
    Metal bindingi222 – 2221Magnesium 2By similarity
    Metal bindingi224 – 2241Magnesium 2By similarity
    Sitei224 – 2241Transition state stabilizerBy similarity
    Sitei328 – 3281Important for catalytic activityBy similarity
    Metal bindingi353 – 3531Magnesium 1By similarity
    Sitei354 – 3541Interaction with DNA substrateBy similarity

    GO - Molecular functioni

    1. DNA-(apurinic or apyrimidinic site) lyase activity Source: SGD
    2. DNA binding Source: InterPro
    3. double-stranded DNA 3'-5' exodeoxyribonuclease activity Source: SGD
    4. phosphoric diester hydrolase activity Source: SGD
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. base-excision repair Source: SGD
    2. DNA catabolic process, endonucleolytic Source: GOC
    3. DNA catabolic process, exonucleolytic Source: GOC

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28922-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-(apurinic or apyrimidinic site) lyase 2 (EC:4.2.99.18)
    Alternative name(s):
    AP endonuclease 2
    Apurinic-apyrimidinic endonuclease 2
    Gene namesi
    Name:APN2
    Synonyms:ETH1
    Ordered Locus Names:YBL019W
    ORF Names:YBL0443
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBL019w.
    SGDiS000000115. APN2.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 520520DNA-(apurinic or apyrimidinic site) lyase 2PRO_0000200020Add
    BLAST

    Proteomic databases

    MaxQBiP38207.
    PaxDbiP38207.
    PRIDEiP38207.

    Expressioni

    Gene expression databases

    GenevestigatoriP38207.

    Interactioni

    Protein-protein interaction databases

    BioGridi32679. 39 interactions.
    DIPiDIP-3930N.
    IntActiP38207. 2 interactions.
    MINTiMINT-515848.
    STRINGi4932.YBL019W.

    Structurei

    3D structure databases

    ProteinModelPortaliP38207.
    SMRiP38207. Positions 17-365.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DNA repair enzymes AP/ExoA family.Curated

    Phylogenomic databases

    eggNOGiCOG0708.
    GeneTreeiENSGT00530000063540.
    HOGENOMiHOG000246560.
    KOiK10772.
    OMAiGRKFWIC.
    OrthoDBiEOG7TTQJT.

    Family and domain databases

    Gene3Di3.60.10.10. 2 hits.
    InterProiIPR004808. AP_endonuc_1.
    IPR020848. AP_endonuclease_F1_CS.
    IPR005135. Endo/exonuclease/phosphatase.
    IPR010666. Znf_GRF.
    [Graphical view]
    PANTHERiPTHR22748. PTHR22748. 1 hit.
    PfamiPF03372. Exo_endo_phos. 1 hit.
    PF06839. zf-GRF. 1 hit.
    [Graphical view]
    SUPFAMiSSF56219. SSF56219. 2 hits.
    PROSITEiPS00728. AP_NUCLEASE_F1_3. 1 hit.
    PS51435. AP_NUCLEASE_F1_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38207-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSENTLLD GKSENTIRFL TFNVNGIRTF FHYQPFSQMN QSLRSVFDFF    50
    RADIITFQEL KTEKLSISKW GRVDGFYSFI SIPQTRKGYS GVGCWIRIPE 100
    KNHPLYHALQ VVKAEEGITG YLTIKNGKHS AISYRNDVNQ GIGGYDSLDP 150
    DLDEKSALEL DSEGRCVMVE LACGIVIISV YCPANSNSSE EGEMFRLRFL 200
    KVLLRRVRNL DKIGKKIVLM GDVNVCRDLI DSADTLEQFS IPITDPMGGT 250
    KLEAQYRDKA IQFIINPDTP HRRIFNQILA DSLLPDASKR GILIDTTRLI 300
    QTRNRLKMYT VWNMLKNLRP SNYGSRIDFI LVSLKLERCI KAADILPDIL 350
    GSDHCPVYSD LDILDDRIEP GTTQVPIPKF EARYKYNLRN HNVLEMFAKK 400
    DTNKESNKQK YCVSKVMNTK KNSNIKNKSL DSFFQKVNGE KDDRIKESSE 450
    IPQQAKKRIS TPKLNFKDVF GKPPLCRHGE ESMLKTSKTS ANPGRKFWIC 500
    KRSRGDSNNT ESSCGFFQWV 520
    Length:520
    Mass (Da):59,445
    Last modified:October 1, 1994 - v1
    Checksum:iE3947C4D904C53FB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti15 – 151N → D in AAT93202. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z35780 Genomic DNA. Translation: CAA84838.1.
    AY693183 Genomic DNA. Translation: AAT93202.1.
    BK006936 Genomic DNA. Translation: DAA07101.1.
    PIRiS45753.
    RefSeqiNP_009534.1. NM_001178259.1.

    Genome annotation databases

    EnsemblFungiiYBL019W; YBL019W; YBL019W.
    GeneIDi852262.
    KEGGisce:YBL019W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z35780 Genomic DNA. Translation: CAA84838.1 .
    AY693183 Genomic DNA. Translation: AAT93202.1 .
    BK006936 Genomic DNA. Translation: DAA07101.1 .
    PIRi S45753.
    RefSeqi NP_009534.1. NM_001178259.1.

    3D structure databases

    ProteinModelPortali P38207.
    SMRi P38207. Positions 17-365.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32679. 39 interactions.
    DIPi DIP-3930N.
    IntActi P38207. 2 interactions.
    MINTi MINT-515848.
    STRINGi 4932.YBL019W.

    Proteomic databases

    MaxQBi P38207.
    PaxDbi P38207.
    PRIDEi P38207.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBL019W ; YBL019W ; YBL019W .
    GeneIDi 852262.
    KEGGi sce:YBL019W.

    Organism-specific databases

    CYGDi YBL019w.
    SGDi S000000115. APN2.

    Phylogenomic databases

    eggNOGi COG0708.
    GeneTreei ENSGT00530000063540.
    HOGENOMi HOG000246560.
    KOi K10772.
    OMAi GRKFWIC.
    OrthoDBi EOG7TTQJT.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28922-MONOMER.

    Miscellaneous databases

    NextBioi 970856.
    PROi P38207.

    Gene expression databases

    Genevestigatori P38207.

    Family and domain databases

    Gene3Di 3.60.10.10. 2 hits.
    InterProi IPR004808. AP_endonuc_1.
    IPR020848. AP_endonuclease_F1_CS.
    IPR005135. Endo/exonuclease/phosphatase.
    IPR010666. Znf_GRF.
    [Graphical view ]
    PANTHERi PTHR22748. PTHR22748. 1 hit.
    Pfami PF03372. Exo_endo_phos. 1 hit.
    PF06839. zf-GRF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56219. SSF56219. 2 hits.
    PROSITEi PS00728. AP_NUCLEASE_F1_3. 1 hit.
    PS51435. AP_NUCLEASE_F1_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Identification of APN2, the Saccharomyces cerevisiae homolog of the major human AP endonuclease HAP1, and its role in the repair of abasic sites."
      Johnson R.E., Torres-Ramos C.A., Izumi T., Mitra S., Prakash S., Prakash L.
      Genes Dev. 12:3137-3143(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. Cited for: CHARACTERIZATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAPN2_YEAST
    AccessioniPrimary (citable) accession number: P38207
    Secondary accession number(s): D6VPY1, E9P926
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 414 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3