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P38207 (APN2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-(apurinic or apyrimidinic site) lyase 2

EC=4.2.99.18
Alternative name(s):
AP endonuclease 2
Apurinic-apyrimidinic endonuclease 2
Gene names
Name:APN2
Synonyms:ETH1
Ordered Locus Names:YBL019W
ORF Names:YBL0443
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA repair enzyme that cleaves apurinic/apyrimidinic (AP) sites and removes 3'-blocking groups present at single strand breaks of damaged DNA.

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Cofactor

Magnesium. Can also utilize manganese. Probably binds two magnesium or manganese ions per subunit By similarity.

Subcellular location

Nucleus.

Miscellaneous

Present with 414 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the DNA repair enzymes AP/ExoA family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 520520DNA-(apurinic or apyrimidinic site) lyase 2
PRO_0000200020

Sites

Active site1811 By similarity
Active site2221Proton donor/acceptor By similarity
Metal binding591Magnesium 1 By similarity
Metal binding2221Magnesium 2 By similarity
Metal binding2241Magnesium 2 By similarity
Metal binding3531Magnesium 1 By similarity
Site2241Transition state stabilizer By similarity
Site3281Important for catalytic activity By similarity
Site3541Interaction with DNA substrate By similarity

Experimental info

Sequence conflict151N → D in AAT93202. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P38207 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: E3947C4D904C53FB

FASTA52059,445
        10         20         30         40         50         60 
MSSSENTLLD GKSENTIRFL TFNVNGIRTF FHYQPFSQMN QSLRSVFDFF RADIITFQEL 

        70         80         90        100        110        120 
KTEKLSISKW GRVDGFYSFI SIPQTRKGYS GVGCWIRIPE KNHPLYHALQ VVKAEEGITG 

       130        140        150        160        170        180 
YLTIKNGKHS AISYRNDVNQ GIGGYDSLDP DLDEKSALEL DSEGRCVMVE LACGIVIISV 

       190        200        210        220        230        240 
YCPANSNSSE EGEMFRLRFL KVLLRRVRNL DKIGKKIVLM GDVNVCRDLI DSADTLEQFS 

       250        260        270        280        290        300 
IPITDPMGGT KLEAQYRDKA IQFIINPDTP HRRIFNQILA DSLLPDASKR GILIDTTRLI 

       310        320        330        340        350        360 
QTRNRLKMYT VWNMLKNLRP SNYGSRIDFI LVSLKLERCI KAADILPDIL GSDHCPVYSD 

       370        380        390        400        410        420 
LDILDDRIEP GTTQVPIPKF EARYKYNLRN HNVLEMFAKK DTNKESNKQK YCVSKVMNTK 

       430        440        450        460        470        480 
KNSNIKNKSL DSFFQKVNGE KDDRIKESSE IPQQAKKRIS TPKLNFKDVF GKPPLCRHGE 

       490        500        510        520 
ESMLKTSKTS ANPGRKFWIC KRSRGDSNNT ESSCGFFQWV 

« Hide

References

« Hide 'large scale' references
[1]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Identification of APN2, the Saccharomyces cerevisiae homolog of the major human AP endonuclease HAP1, and its role in the repair of abasic sites."
Johnson R.E., Torres-Ramos C.A., Izumi T., Mitra S., Prakash S., Prakash L.
Genes Dev. 12:3137-3143(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Apurinic endonuclease activity of yeast Apn2 protein."
Unk I., Haracska L., Johnson R.E., Prakash S., Prakash L.
J. Biol. Chem. 275:22427-22434(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z35780 Genomic DNA. Translation: CAA84838.1.
AY693183 Genomic DNA. Translation: AAT93202.1.
BK006936 Genomic DNA. Translation: DAA07101.1.
PIRS45753.
RefSeqNP_009534.1. NM_001178259.1.

3D structure databases

ProteinModelPortalP38207.
SMRP38207. Positions 17-365.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32679. 39 interactions.
DIPDIP-3930N.
IntActP38207. 2 interactions.
MINTMINT-515848.
STRING4932.YBL019W.

Proteomic databases

PaxDbP38207.
PRIDEP38207.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL019W; YBL019W; YBL019W.
GeneID852262.
KEGGsce:YBL019W.

Organism-specific databases

CYGDYBL019w.
SGDS000000115. APN2.

Phylogenomic databases

eggNOGCOG0708.
GeneTreeENSGT00530000063540.
HOGENOMHOG000246560.
KOK10772.
OMADAINIAC.
OrthoDBEOG7TTQJT.

Enzyme and pathway databases

BioCycYEAST:G3O-28922-MONOMER.

Gene expression databases

GenevestigatorP38207.

Family and domain databases

Gene3D3.60.10.10. 2 hits.
InterProIPR004808. AP_endonuc_1.
IPR020848. AP_endonuclease_F1_CS.
IPR005135. Endo/exonuclease/phosphatase.
IPR010666. Znf_GRF.
[Graphical view]
PANTHERPTHR22748. PTHR22748. 1 hit.
PfamPF03372. Exo_endo_phos. 1 hit.
PF06839. zf-GRF. 1 hit.
[Graphical view]
SUPFAMSSF56219. SSF56219. 2 hits.
PROSITEPS00728. AP_NUCLEASE_F1_3. 1 hit.
PS51435. AP_NUCLEASE_F1_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio970856.
PROP38207.

Entry information

Entry nameAPN2_YEAST
AccessionPrimary (citable) accession number: P38207
Secondary accession number(s): D6VPY1, E9P926
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 16, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families