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Protein

Oligosaccharide translocation protein RFT1

Gene

RFT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in N-linked oligosaccharide assembly. Required for the translocation of Man5GlcNAc(2)-PP-dolichol from the cytoplasmic side to the lumenal side of the endoplasmic reticulum membrane.1 Publication

GO - Molecular functioni

GO - Biological processi

  • carbohydrate transport Source: UniProtKB-KW
  • dolichol-linked oligosaccharide biosynthetic process Source: Reactome
  • glycolipid translocation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

BioCyciMetaCyc:G3O-28923-MONOMER.
YEAST:G3O-28923-MONOMER.
ReactomeiR-SCE-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Protein family/group databases

TCDBi2.A.66.3.1. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligosaccharide translocation protein RFT1
Alternative name(s):
Requiring fifty-three protein 1
Gene namesi
Name:RFT1
Ordered Locus Names:YBL020W
ORF Names:YBL0442
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL020W.
SGDiS000000116. RFT1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24LumenalSequence analysisAdd BLAST24
Transmembranei25 – 45HelicalSequence analysisAdd BLAST21
Topological domaini46 – 48CytoplasmicSequence analysis3
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Topological domaini70 – 110LumenalSequence analysisAdd BLAST41
Transmembranei111 – 131HelicalSequence analysisAdd BLAST21
Topological domaini132 – 148CytoplasmicSequence analysisAdd BLAST17
Transmembranei149 – 169HelicalSequence analysisAdd BLAST21
Topological domaini170 – 181LumenalSequence analysisAdd BLAST12
Transmembranei182 – 202HelicalSequence analysisAdd BLAST21
Topological domaini203 – 218CytoplasmicSequence analysisAdd BLAST16
Transmembranei219 – 239HelicalSequence analysisAdd BLAST21
Topological domaini240 – 319LumenalSequence analysisAdd BLAST80
Transmembranei320 – 340HelicalSequence analysisAdd BLAST21
Topological domaini341 – 372CytoplasmicSequence analysisAdd BLAST32
Transmembranei373 – 393HelicalSequence analysisAdd BLAST21
Topological domaini394 – 413LumenalSequence analysisAdd BLAST20
Transmembranei414 – 434HelicalSequence analysisAdd BLAST21
Topological domaini435 – 443CytoplasmicSequence analysis9
Transmembranei444 – 464HelicalSequence analysisAdd BLAST21
Topological domaini465 – 469LumenalSequence analysis5
Transmembranei470 – 490HelicalSequence analysisAdd BLAST21
Topological domaini491 – 509CytoplasmicSequence analysisAdd BLAST19
Transmembranei510 – 530HelicalSequence analysisAdd BLAST21
Topological domaini531 – 532LumenalSequence analysis2
Transmembranei533 – 553HelicalSequence analysisAdd BLAST21
Topological domaini554 – 574CytoplasmicSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002124201 – 574Oligosaccharide translocation protein RFT1Add BLAST574

Proteomic databases

MaxQBiP38206.
PRIDEiP38206.

Interactioni

Protein-protein interaction databases

BioGridi32678. 23 interactors.
DIPiDIP-3885N.
IntActiP38206. 5 interactors.
MINTiMINT-532586.

Structurei

3D structure databases

ProteinModelPortaliP38206.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RFT1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000011390.
HOGENOMiHOG000000715.
InParanoidiP38206.
KOiK06316.
OMAiLCCEQGW.
OrthoDBiEOG092C2P9A.

Family and domain databases

InterProiIPR007594. RFT1.
[Graphical view]
PANTHERiPTHR13117. PTHR13117. 1 hit.
PfamiPF04506. Rft-1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38206-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKNSQLPS TSEQILERST TGATFLMMGQ LFTKLVTFIL NNLLIRFLSP
60 70 80 90 100
RIFGITAFLE FIQGTVLFFS RDAIRLSTLR ISDSGNGIID DDDEEEYQET
110 120 130 140 150
HYKSKVLQTA VNFAYIPFWI GFPLSIGLIA WQYRNINAYF ITLPFFRWSI
160 170 180 190 200
FLIWLSIIVE LLSEPFFIVN QFMLNYAARS RFESIAVTTG CIVNFIVVYA
210 220 230 240 250
VQQSRYPMGV VTSDIDKEGI AILAFALGKL AHSITLLACY YWDYLKNFKP
260 270 280 290 300
KKLFSTRLTK IKTRENNELK KGYPKSTSYF FQNDILQHFK KVYFQLCFKH
310 320 330 340 350
LLTEGDKLII NSLCTVEEQG IYALLSNYGS LLTRLLFAPI EESLRLFLAR
360 370 380 390 400
LLSSHNPKNL KLSIEVLVNL TRFYIYLSLM IIVFGPANSS FLLQFLIGSK
410 420 430 440 450
WSTTSVLDTI RVYCFYIPFL SLNGIFEAFF QSVATGDQIL KHSYFMMAFS
460 470 480 490 500
GIFLLNSWLL IEKLKLSIEG LILSNIINMV LRILYCGVFL NKFHRELFTD
510 520 530 540 550
SSFFFNFKDF KTVIIAGSTI CLLDWWFIGY VKNLQQFVVN VLFAMGLLAL
560 570
ILVKERQTIQ SFINKRAVSN SKDV
Length:574
Mass (Da):66,211
Last modified:October 1, 1994 - v1
Checksum:iD619A5362FB65509
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74 – 75IR → MF in AAA53537 (PubMed:2832732).Curated2
Sequence conflicti207 – 216PMGVVTSDID → QWGLSHRTLT in AAA53537 (PubMed:2832732).Curated10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15087 Genomic DNA. Translation: AAA86312.1.
Z35781 Genomic DNA. Translation: CAA84839.1.
M20318 Genomic DNA. Translation: AAA53537.1.
BK006936 Genomic DNA. Translation: DAA07100.1.
PIRiS45754.
RefSeqiNP_009533.1. NM_001178260.1.

Genome annotation databases

EnsemblFungiiYBL020W; YBL020W; YBL020W.
GeneIDi852261.
KEGGisce:YBL020W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15087 Genomic DNA. Translation: AAA86312.1.
Z35781 Genomic DNA. Translation: CAA84839.1.
M20318 Genomic DNA. Translation: AAA53537.1.
BK006936 Genomic DNA. Translation: DAA07100.1.
PIRiS45754.
RefSeqiNP_009533.1. NM_001178260.1.

3D structure databases

ProteinModelPortaliP38206.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32678. 23 interactors.
DIPiDIP-3885N.
IntActiP38206. 5 interactors.
MINTiMINT-532586.

Protein family/group databases

TCDBi2.A.66.3.1. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Proteomic databases

MaxQBiP38206.
PRIDEiP38206.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL020W; YBL020W; YBL020W.
GeneIDi852261.
KEGGisce:YBL020W.

Organism-specific databases

EuPathDBiFungiDB:YBL020W.
SGDiS000000116. RFT1.

Phylogenomic databases

GeneTreeiENSGT00390000011390.
HOGENOMiHOG000000715.
InParanoidiP38206.
KOiK06316.
OMAiLCCEQGW.
OrthoDBiEOG092C2P9A.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-28923-MONOMER.
YEAST:G3O-28923-MONOMER.
ReactomeiR-SCE-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiP38206.

Family and domain databases

InterProiIPR007594. RFT1.
[Graphical view]
PANTHERiPTHR13117. PTHR13117. 1 hit.
PfamiPF04506. Rft-1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRFT1_YEAST
AccessioniPrimary (citable) accession number: P38206
Secondary accession number(s): D6VPY0, Q05814
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.