Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Oligosaccharide translocation protein RFT1

Gene

RFT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in N-linked oligosaccharide assembly. Required for the translocation of Man5GlcNAc(2)-PP-dolichol from the cytoplasmic side to the lumenal side of the endoplasmic reticulum membrane.1 Publication

GO - Molecular functioni

GO - Biological processi

  • carbohydrate transport Source: UniProtKB-KW
  • glycolipid translocation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-28923-MONOMER.

Protein family/group databases

TCDBi2.A.66.3.1. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligosaccharide translocation protein RFT1
Alternative name(s):
Requiring fifty-three protein 1
Gene namesi
Name:RFT1
Ordered Locus Names:YBL020W
ORF Names:YBL0442
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL020W.
SGDiS000000116. RFT1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424LumenalSequence analysisAdd
BLAST
Transmembranei25 – 4521HelicalSequence analysisAdd
BLAST
Topological domaini46 – 483CytoplasmicSequence analysis
Transmembranei49 – 6921HelicalSequence analysisAdd
BLAST
Topological domaini70 – 11041LumenalSequence analysisAdd
BLAST
Transmembranei111 – 13121HelicalSequence analysisAdd
BLAST
Topological domaini132 – 14817CytoplasmicSequence analysisAdd
BLAST
Transmembranei149 – 16921HelicalSequence analysisAdd
BLAST
Topological domaini170 – 18112LumenalSequence analysisAdd
BLAST
Transmembranei182 – 20221HelicalSequence analysisAdd
BLAST
Topological domaini203 – 21816CytoplasmicSequence analysisAdd
BLAST
Transmembranei219 – 23921HelicalSequence analysisAdd
BLAST
Topological domaini240 – 31980LumenalSequence analysisAdd
BLAST
Transmembranei320 – 34021HelicalSequence analysisAdd
BLAST
Topological domaini341 – 37232CytoplasmicSequence analysisAdd
BLAST
Transmembranei373 – 39321HelicalSequence analysisAdd
BLAST
Topological domaini394 – 41320LumenalSequence analysisAdd
BLAST
Transmembranei414 – 43421HelicalSequence analysisAdd
BLAST
Topological domaini435 – 4439CytoplasmicSequence analysis
Transmembranei444 – 46421HelicalSequence analysisAdd
BLAST
Topological domaini465 – 4695LumenalSequence analysis
Transmembranei470 – 49021HelicalSequence analysisAdd
BLAST
Topological domaini491 – 50919CytoplasmicSequence analysisAdd
BLAST
Transmembranei510 – 53021HelicalSequence analysisAdd
BLAST
Topological domaini531 – 5322LumenalSequence analysis
Transmembranei533 – 55321HelicalSequence analysisAdd
BLAST
Topological domaini554 – 57421CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574Oligosaccharide translocation protein RFT1PRO_0000212420Add
BLAST

Proteomic databases

MaxQBiP38206.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CCDC155Q8N6L03EBI-15028,EBI-749265From a different organism.
RASGRP3Q8IV613EBI-15028,EBI-1047876From a different organism.

Protein-protein interaction databases

BioGridi32678. 23 interactions.
DIPiDIP-3885N.
IntActiP38206. 5 interactions.
MINTiMINT-532586.

Structurei

3D structure databases

ProteinModelPortaliP38206.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RFT1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000011390.
HOGENOMiHOG000000715.
InParanoidiP38206.
KOiK06316.
OMAiLCCEQGW.
OrthoDBiEOG7NSBBG.

Family and domain databases

InterProiIPR007594. RFT1.
[Graphical view]
PANTHERiPTHR13117. PTHR13117. 1 hit.
PfamiPF04506. Rft-1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38206-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKNSQLPS TSEQILERST TGATFLMMGQ LFTKLVTFIL NNLLIRFLSP
60 70 80 90 100
RIFGITAFLE FIQGTVLFFS RDAIRLSTLR ISDSGNGIID DDDEEEYQET
110 120 130 140 150
HYKSKVLQTA VNFAYIPFWI GFPLSIGLIA WQYRNINAYF ITLPFFRWSI
160 170 180 190 200
FLIWLSIIVE LLSEPFFIVN QFMLNYAARS RFESIAVTTG CIVNFIVVYA
210 220 230 240 250
VQQSRYPMGV VTSDIDKEGI AILAFALGKL AHSITLLACY YWDYLKNFKP
260 270 280 290 300
KKLFSTRLTK IKTRENNELK KGYPKSTSYF FQNDILQHFK KVYFQLCFKH
310 320 330 340 350
LLTEGDKLII NSLCTVEEQG IYALLSNYGS LLTRLLFAPI EESLRLFLAR
360 370 380 390 400
LLSSHNPKNL KLSIEVLVNL TRFYIYLSLM IIVFGPANSS FLLQFLIGSK
410 420 430 440 450
WSTTSVLDTI RVYCFYIPFL SLNGIFEAFF QSVATGDQIL KHSYFMMAFS
460 470 480 490 500
GIFLLNSWLL IEKLKLSIEG LILSNIINMV LRILYCGVFL NKFHRELFTD
510 520 530 540 550
SSFFFNFKDF KTVIIAGSTI CLLDWWFIGY VKNLQQFVVN VLFAMGLLAL
560 570
ILVKERQTIQ SFINKRAVSN SKDV
Length:574
Mass (Da):66,211
Last modified:October 1, 1994 - v1
Checksum:iD619A5362FB65509
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 752IR → MF in AAA53537 (PubMed:2832732).Curated
Sequence conflicti207 – 21610PMGVVTSDID → QWGLSHRTLT in AAA53537 (PubMed:2832732).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15087 Genomic DNA. Translation: AAA86312.1.
Z35781 Genomic DNA. Translation: CAA84839.1.
M20318 Genomic DNA. Translation: AAA53537.1.
BK006936 Genomic DNA. Translation: DAA07100.1.
PIRiS45754.
RefSeqiNP_009533.1. NM_001178260.1.

Genome annotation databases

EnsemblFungiiYBL020W; YBL020W; YBL020W.
GeneIDi852261.
KEGGisce:YBL020W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15087 Genomic DNA. Translation: AAA86312.1.
Z35781 Genomic DNA. Translation: CAA84839.1.
M20318 Genomic DNA. Translation: AAA53537.1.
BK006936 Genomic DNA. Translation: DAA07100.1.
PIRiS45754.
RefSeqiNP_009533.1. NM_001178260.1.

3D structure databases

ProteinModelPortaliP38206.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32678. 23 interactions.
DIPiDIP-3885N.
IntActiP38206. 5 interactions.
MINTiMINT-532586.

Protein family/group databases

TCDBi2.A.66.3.1. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Proteomic databases

MaxQBiP38206.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL020W; YBL020W; YBL020W.
GeneIDi852261.
KEGGisce:YBL020W.

Organism-specific databases

EuPathDBiFungiDB:YBL020W.
SGDiS000000116. RFT1.

Phylogenomic databases

GeneTreeiENSGT00390000011390.
HOGENOMiHOG000000715.
InParanoidiP38206.
KOiK06316.
OMAiLCCEQGW.
OrthoDBiEOG7NSBBG.

Enzyme and pathway databases

BioCyciYEAST:G3O-28923-MONOMER.

Miscellaneous databases

NextBioi970853.
PROiP38206.

Family and domain databases

InterProiIPR007594. RFT1.
[Graphical view]
PANTHERiPTHR13117. PTHR13117. 1 hit.
PfamiPF04506. Rft-1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Suppression of the yeast mutation rft1-1 by human p53."
    Koerte A., Chong T., Li X., Wahane K., Cai M.
    J. Biol. Chem. 270:22556-22564(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YMW2.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The HAP3 regulatory locus of Saccharomyces cerevisiae encodes divergent overlapping transcripts."
    Hahn S., Pinkham J., Wei R., Miller R., Guarente L.
    Mol. Cell. Biol. 8:655-663(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-428.
  5. "Translocation of lipid-linked oligosaccharides across the ER membrane requires Rft1 protein."
    Helenius J., Ng D.T.W., Marolda C.L., Walter P., Valvano M.A., Aebi M.
    Nature 415:447-450(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRFT1_YEAST
AccessioniPrimary (citable) accession number: P38206
Secondary accession number(s): D6VPY0, Q05814
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 11, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.