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P38199

- HEK2_YEAST

UniProt

P38199 - HEK2_YEAST

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Protein

Heterogeneous nuclear rnp K-like protein 2

Gene

HEK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

RNA-binding protein involved in the correct localization of transcripts in the cell. RNA localization is a widespread mechanism for achieving localized protein synthesis. Required for the asymmetric localization to the daughter cell nucleus of the ASH1 transcript, coding for a specific repressor of transcription. Overexpression inhibits translation of the ASH1 transcript. Involved in the stability of transcripts such as the MTL1 mRNA. Involved in structural and functional organization of telomeric chromatin and regulates silencing at the HMR locus.6 Publications

GO - Molecular functioni

  1. mRNA binding Source: SGD

GO - Biological processi

  1. chromatin modification Source: UniProtKB-KW
  2. intracellular mRNA localization Source: SGD
  3. mRNA stabilization Source: SGD
  4. mRNA transport Source: UniProtKB-KW
  5. regulation of translation Source: UniProtKB-KW
  6. telomere maintenance via telomerase Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

mRNA transport, Translation regulation, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28935-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear rnp K-like protein 2
Alternative name(s):
KH domain-containing protein 1
Gene namesi
Name:HEK2
Synonyms:KHD1
Ordered Locus Names:YBL032W
ORF Names:YBL0418
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBL032w.
SGDiS000000128. HEK2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. cytoplasmic mRNA processing body Source: SGD
  3. nuclear chromosome, telomeric region Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 381381Heterogeneous nuclear rnp K-like protein 2PRO_0000050161Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei358 – 3581Phosphoserine1 Publication
Modified residuei360 – 3601Phosphoserine2 Publications
Modified residuei362 – 3621Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by the plasma membrane-anchored casein kinase YCK1. Phosphorylation at its C-terminus reduces its RNA-binding capacity.4 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38199.
PaxDbiP38199.
PeptideAtlasiP38199.

Expressioni

Gene expression databases

GenevestigatoriP38199.

Interactioni

Subunit structurei

Binds RNA.

Protein-protein interaction databases

BioGridi32665. 1154 interactions.
DIPiDIP-3934N.
IntActiP38199. 51 interactions.
MINTiMINT-506819.
STRINGi4932.YBL032W.

Structurei

3D structure databases

ProteinModelPortaliP38199.
SMRiP38199. Positions 43-226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 10765KH 1PROSITE-ProRule annotationAdd
BLAST
Domaini156 – 22166KH 2PROSITE-ProRule annotationAdd
BLAST
Domaini258 – 32669KH 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the HEK2 family.Curated
Contains 3 KH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG294694.
InParanoidiP38199.
OMAiVANCINE.
OrthoDBiEOG77HDRG.

Family and domain databases

Gene3Di3.30.1370.10. 3 hits.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF00013. KH_1. 3 hits.
PF13014. KH_3. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 3 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 3 hits.
PROSITEiPS50084. KH_TYPE_1. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38199-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQFFEAATP VAIPTNNTNG GSSDAGSAAT GGAPVVGTTA QPTINHRLLL
60 70 80 90 100
SLKEAAKIIG TKGSTISRIR AANAVKIGIS EKVPGCSDRI LSCAGNVINV
110 120 130 140 150
ANAIGDIVDV LNKRNPENED AAEGEAEEHY YFHFLNHILP APSKDEIRDL
160 170 180 190 200
QQLEDIGYVR LIVANSHISS IIGKAGATIK SLINKHGVKI VASKDFLPAS
210 220 230 240 250
DERIIEIQGF PGSITNVLIE ISEIILSDVD VRFSTERSYF PHLKKSSGEP
260 270 280 290 300
TSPSTSSNTR IELKIPELYV GAIIGRGMNR IKNLKTFTKT NIVVERKDDD
310 320 330 340 350
DKDENFRKFI ITSKFPKNVK LAESMLLKNL NTEIEKRENY KRKLEAAEGD
360 370 380
ATVVTERSDS ASFLEEKEEP QENHDNKEEQ S
Length:381
Mass (Da):41,683
Last modified:October 1, 1994 - v1
Checksum:i0A8752961EB77DC9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti362 – 3621S → P in AAT92833. (PubMed:17322287)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77291 Genomic DNA. Translation: CAA54496.1.
Z35793 Genomic DNA. Translation: CAA84852.1.
AY692814 Genomic DNA. Translation: AAT92833.1.
BK006936 Genomic DNA. Translation: DAA07087.1.
PIRiS45766.
RefSeqiNP_009521.1. NM_001178272.1.

Genome annotation databases

EnsemblFungiiYBL032W; YBL032W; YBL032W.
GeneIDi852248.
KEGGisce:YBL032W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77291 Genomic DNA. Translation: CAA54496.1 .
Z35793 Genomic DNA. Translation: CAA84852.1 .
AY692814 Genomic DNA. Translation: AAT92833.1 .
BK006936 Genomic DNA. Translation: DAA07087.1 .
PIRi S45766.
RefSeqi NP_009521.1. NM_001178272.1.

3D structure databases

ProteinModelPortali P38199.
SMRi P38199. Positions 43-226.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32665. 1154 interactions.
DIPi DIP-3934N.
IntActi P38199. 51 interactions.
MINTi MINT-506819.
STRINGi 4932.YBL032W.

Proteomic databases

MaxQBi P38199.
PaxDbi P38199.
PeptideAtlasi P38199.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBL032W ; YBL032W ; YBL032W .
GeneIDi 852248.
KEGGi sce:YBL032W.

Organism-specific databases

CYGDi YBL032w.
SGDi S000000128. HEK2.

Phylogenomic databases

eggNOGi NOG294694.
InParanoidi P38199.
OMAi VANCINE.
OrthoDBi EOG77HDRG.

Enzyme and pathway databases

BioCyci YEAST:G3O-28935-MONOMER.

Miscellaneous databases

NextBioi 970814.
PROi P38199.

Gene expression databases

Genevestigatori P38199.

Family and domain databases

Gene3Di 3.30.1370.10. 3 hits.
InterProi IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view ]
Pfami PF00013. KH_1. 3 hits.
PF13014. KH_3. 1 hit.
[Graphical view ]
SMARTi SM00322. KH. 3 hits.
[Graphical view ]
SUPFAMi SSF54791. SSF54791. 3 hits.
PROSITEi PS50084. KH_TYPE_1. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 17.4 kb DNA segment of yeast chromosome II encompassing the ribosomal protein L19 as well as proteins with homologies to components of the hnRNP and snRNP complexes and to the human proliferation-associated p120 antigen."
    van Dyck L., Jonniaux J.-L., Barreiros T.D.M., Kleine K., Goffeau A.
    Yeast 10:1663-1673(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "KH domain-containing proteins of yeast: absence of a fragile X gene homologue."
    Currie J.R., Brown W.T.
    Am. J. Med. Genet. 84:272-276(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.
  6. "The Khd1 protein, which has three KH RNA-binding motifs, is required for proper localization of ASH1 mRNA in yeast."
    Irie K., Tadauchi T., Takizawa P.A., Vale R.D., Matsumoto K., Herskowitz I.
    EMBO J. 21:1158-1167(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA-BINDING, FUNCTION.
  7. "Yeast hnRNP K-like genes are involved in regulation of the telomeric position effect and telomere length."
    Denisenko O., Bomsztyk K.
    Mol. Cell. Biol. 22:286-297(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "The binding activity of yeast RNAs to yeast Hek2p and mammalian hnRNP K proteins, determined using the three-hybrid system."
    Paziewska A., Wyrwicz L.S., Ostrowski J.
    Cell. Mol. Biol. Lett. 10:227-235(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA-BINDING.
  11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  12. "Local activation of yeast ASH1 mRNA translation through phosphorylation of Khd1p by the casein kinase Yck1p."
    Paquin N., Menade M., Poirier G., Donato D., Drouet E., Chartrand P.
    Mol. Cell 26:795-809(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, RNA-BINDING, PHOSPHORYLATION.
  13. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Epistatic interaction between the K-homology domain protein HEK2 and SIR1 at HMR and telomeres in yeast."
    Denisenko O., Bomsztyk K.
    J. Mol. Biol. 375:1178-1187(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  15. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360 AND SER-362, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. "Distinct roles for Khd1p in the localization and expression of bud-localized mRNAs in yeast."
    Hasegawa Y., Irie K., Gerber A.P.
    RNA 14:2333-2347(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, RNA-BINDING.
  17. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  18. "Stability control of MTL1 mRNA by the RNA-binding protein Khd1p in yeast."
    Mauchi N., Ohtake Y., Irie K.
    Cell Struct. Funct. 35:95-105(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiHEK2_YEAST
AccessioniPrimary (citable) accession number: P38199
Secondary accession number(s): D6VPW7, E9P8X5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3