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P38199 (HEK2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Heterogeneous nuclear rnp K-like protein 2
Alternative name(s):
KH domain-containing protein 1
Gene names
Name:HEK2
Synonyms:KHD1
Ordered Locus Names:YBL032W
ORF Names:YBL0418
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA-binding protein involved in the correct localization of transcripts in the cell. RNA localization is a widespread mechanism for achieving localized protein synthesis. Required for the asymmetric localization to the daughter cell nucleus of the ASH1 transcript, coding for a specific repressor of transcription. Overexpression inhibits translation of the ASH1 transcript. Involved in the stability of transcripts such as the MTL1 mRNA. Involved in structural and functional organization of telomeric chromatin and regulates silencing at the HMR locus. Ref.6 Ref.7 Ref.12 Ref.14 Ref.16 Ref.18

Subunit structure

Binds RNA.

Subcellular location

Cytoplasm. CytoplasmP-body. Nucleus. Chromosometelomere Ref.7 Ref.8 Ref.14 Ref.18.

Post-translational modification

Phosphorylated by the plasma membrane-anchored casein kinase YCK1. Phosphorylation at its C-terminus reduces its RNA-binding capacity. Ref.12

Miscellaneous

Present with 15600 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the HEK2 family.

Contains 3 KH domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 381381Heterogeneous nuclear rnp K-like protein 2
PRO_0000050161

Regions

Domain43 – 10765KH 1
Domain156 – 22166KH 2
Domain258 – 32669KH 3

Amino acid modifications

Modified residue3581Phosphoserine Ref.13
Modified residue3601Phosphoserine Ref.15 Ref.17
Modified residue3621Phosphoserine Ref.15

Experimental info

Sequence conflict3621S → P in AAT92833. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P38199 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 0A8752961EB77DC9

FASTA38141,683
        10         20         30         40         50         60 
MSQFFEAATP VAIPTNNTNG GSSDAGSAAT GGAPVVGTTA QPTINHRLLL SLKEAAKIIG 

        70         80         90        100        110        120 
TKGSTISRIR AANAVKIGIS EKVPGCSDRI LSCAGNVINV ANAIGDIVDV LNKRNPENED 

       130        140        150        160        170        180 
AAEGEAEEHY YFHFLNHILP APSKDEIRDL QQLEDIGYVR LIVANSHISS IIGKAGATIK 

       190        200        210        220        230        240 
SLINKHGVKI VASKDFLPAS DERIIEIQGF PGSITNVLIE ISEIILSDVD VRFSTERSYF 

       250        260        270        280        290        300 
PHLKKSSGEP TSPSTSSNTR IELKIPELYV GAIIGRGMNR IKNLKTFTKT NIVVERKDDD 

       310        320        330        340        350        360 
DKDENFRKFI ITSKFPKNVK LAESMLLKNL NTEIEKRENY KRKLEAAEGD ATVVTERSDS 

       370        380 
ASFLEEKEEP QENHDNKEEQ S 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of a 17.4 kb DNA segment of yeast chromosome II encompassing the ribosomal protein L19 as well as proteins with homologies to components of the hnRNP and snRNP complexes and to the human proliferation-associated p120 antigen."
van Dyck L., Jonniaux J.-L., Barreiros T.D.M., Kleine K., Goffeau A.
Yeast 10:1663-1673(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"KH domain-containing proteins of yeast: absence of a fragile X gene homologue."
Currie J.R., Brown W.T.
Am. J. Med. Genet. 84:272-276(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN.
[6]"The Khd1 protein, which has three KH RNA-binding motifs, is required for proper localization of ASH1 mRNA in yeast."
Irie K., Tadauchi T., Takizawa P.A., Vale R.D., Matsumoto K., Herskowitz I.
EMBO J. 21:1158-1167(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: RNA-BINDING, FUNCTION.
[7]"Yeast hnRNP K-like genes are involved in regulation of the telomeric position effect and telomere length."
Denisenko O., Bomsztyk K.
Mol. Cell. Biol. 22:286-297(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"The binding activity of yeast RNAs to yeast Hek2p and mammalian hnRNP K proteins, determined using the three-hybrid system."
Paziewska A., Wyrwicz L.S., Ostrowski J.
Cell. Mol. Biol. Lett. 10:227-235(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: RNA-BINDING.
[11]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[12]"Local activation of yeast ASH1 mRNA translation through phosphorylation of Khd1p by the casein kinase Yck1p."
Paquin N., Menade M., Poirier G., Donato D., Drouet E., Chartrand P.
Mol. Cell 26:795-809(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, RNA-BINDING, PHOSPHORYLATION.
[13]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Epistatic interaction between the K-homology domain protein HEK2 and SIR1 at HMR and telomeres in yeast."
Denisenko O., Bomsztyk K.
J. Mol. Biol. 375:1178-1187(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[15]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360 AND SER-362, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"Distinct roles for Khd1p in the localization and expression of bud-localized mRNAs in yeast."
Hasegawa Y., Irie K., Gerber A.P.
RNA 14:2333-2347(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, RNA-BINDING.
[17]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[18]"Stability control of MTL1 mRNA by the RNA-binding protein Khd1p in yeast."
Mauchi N., Ohtake Y., Irie K.
Cell Struct. Funct. 35:95-105(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77291 Genomic DNA. Translation: CAA54496.1.
Z35793 Genomic DNA. Translation: CAA84852.1.
AY692814 Genomic DNA. Translation: AAT92833.1.
BK006936 Genomic DNA. Translation: DAA07087.1.
PIRS45766.
RefSeqNP_009521.1. NM_001178272.1.

3D structure databases

ProteinModelPortalP38199.
SMRP38199. Positions 43-226, 258-331.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32665. 1153 interactions.
DIPDIP-3934N.
IntActP38199. 51 interactions.
MINTMINT-506819.
STRING4932.YBL032W.

Proteomic databases

PaxDbP38199.
PeptideAtlasP38199.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL032W; YBL032W; YBL032W.
GeneID852248.
KEGGsce:YBL032W.

Organism-specific databases

CYGDYBL032w.
SGDS000000128. HEK2.

Phylogenomic databases

eggNOGNOG294694.
OMANSHISSI.
OrthoDBEOG77HDRG.

Enzyme and pathway databases

BioCycYEAST:G3O-28935-MONOMER.

Gene expression databases

GenevestigatorP38199.

Family and domain databases

Gene3D3.30.1370.10. 3 hits.
InterProIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamPF00013. KH_1. 3 hits.
PF13014. KH_3. 1 hit.
[Graphical view]
SMARTSM00322. KH. 3 hits.
[Graphical view]
SUPFAMSSF54791. SSF54791. 3 hits.
PROSITEPS50084. KH_TYPE_1. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio970814.
PROP38199.

Entry information

Entry nameHEK2_YEAST
AccessionPrimary (citable) accession number: P38199
Secondary accession number(s): D6VPW7, E9P8X5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families