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P38199

- HEK2_YEAST

UniProt

P38199 - HEK2_YEAST

Protein

Heterogeneous nuclear rnp K-like protein 2

Gene

HEK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    RNA-binding protein involved in the correct localization of transcripts in the cell. RNA localization is a widespread mechanism for achieving localized protein synthesis. Required for the asymmetric localization to the daughter cell nucleus of the ASH1 transcript, coding for a specific repressor of transcription. Overexpression inhibits translation of the ASH1 transcript. Involved in the stability of transcripts such as the MTL1 mRNA. Involved in structural and functional organization of telomeric chromatin and regulates silencing at the HMR locus.6 Publications

    GO - Molecular functioni

    1. mRNA binding Source: SGD

    GO - Biological processi

    1. chromatin modification Source: UniProtKB-KW
    2. intracellular mRNA localization Source: SGD
    3. mRNA stabilization Source: SGD
    4. mRNA transport Source: UniProtKB-KW
    5. regulation of translation Source: UniProtKB-KW
    6. telomere maintenance via telomerase Source: SGD

    Keywords - Molecular functioni

    Chromatin regulator

    Keywords - Biological processi

    mRNA transport, Translation regulation, Transport

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28935-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heterogeneous nuclear rnp K-like protein 2
    Alternative name(s):
    KH domain-containing protein 1
    Gene namesi
    Name:HEK2
    Synonyms:KHD1
    Ordered Locus Names:YBL032W
    ORF Names:YBL0418
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBL032w.
    SGDiS000000128. HEK2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. cytoplasmic mRNA processing body Source: SGD
    3. nuclear chromosome, telomeric region Source: SGD

    Keywords - Cellular componenti

    Chromosome, Cytoplasm, Nucleus, Telomere

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 381381Heterogeneous nuclear rnp K-like protein 2PRO_0000050161Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei358 – 3581Phosphoserine2 Publications
    Modified residuei360 – 3601Phosphoserine3 Publications
    Modified residuei362 – 3621Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylated by the plasma membrane-anchored casein kinase YCK1. Phosphorylation at its C-terminus reduces its RNA-binding capacity.4 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38199.
    PaxDbiP38199.
    PeptideAtlasiP38199.

    Expressioni

    Gene expression databases

    GenevestigatoriP38199.

    Interactioni

    Subunit structurei

    Binds RNA.

    Protein-protein interaction databases

    BioGridi32665. 1153 interactions.
    DIPiDIP-3934N.
    IntActiP38199. 51 interactions.
    MINTiMINT-506819.
    STRINGi4932.YBL032W.

    Structurei

    3D structure databases

    ProteinModelPortaliP38199.
    SMRiP38199. Positions 43-226.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 10765KH 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini156 – 22166KH 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini258 – 32669KH 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the HEK2 family.Curated
    Contains 3 KH domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG294694.
    OMAiVANCINE.
    OrthoDBiEOG77HDRG.

    Family and domain databases

    Gene3Di3.30.1370.10. 3 hits.
    InterProiIPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    [Graphical view]
    PfamiPF00013. KH_1. 3 hits.
    PF13014. KH_3. 1 hit.
    [Graphical view]
    SMARTiSM00322. KH. 3 hits.
    [Graphical view]
    SUPFAMiSSF54791. SSF54791. 3 hits.
    PROSITEiPS50084. KH_TYPE_1. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38199-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQFFEAATP VAIPTNNTNG GSSDAGSAAT GGAPVVGTTA QPTINHRLLL    50
    SLKEAAKIIG TKGSTISRIR AANAVKIGIS EKVPGCSDRI LSCAGNVINV 100
    ANAIGDIVDV LNKRNPENED AAEGEAEEHY YFHFLNHILP APSKDEIRDL 150
    QQLEDIGYVR LIVANSHISS IIGKAGATIK SLINKHGVKI VASKDFLPAS 200
    DERIIEIQGF PGSITNVLIE ISEIILSDVD VRFSTERSYF PHLKKSSGEP 250
    TSPSTSSNTR IELKIPELYV GAIIGRGMNR IKNLKTFTKT NIVVERKDDD 300
    DKDENFRKFI ITSKFPKNVK LAESMLLKNL NTEIEKRENY KRKLEAAEGD 350
    ATVVTERSDS ASFLEEKEEP QENHDNKEEQ S 381
    Length:381
    Mass (Da):41,683
    Last modified:October 1, 1994 - v1
    Checksum:i0A8752961EB77DC9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti362 – 3621S → P in AAT92833. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77291 Genomic DNA. Translation: CAA54496.1.
    Z35793 Genomic DNA. Translation: CAA84852.1.
    AY692814 Genomic DNA. Translation: AAT92833.1.
    BK006936 Genomic DNA. Translation: DAA07087.1.
    PIRiS45766.
    RefSeqiNP_009521.1. NM_001178272.1.

    Genome annotation databases

    EnsemblFungiiYBL032W; YBL032W; YBL032W.
    GeneIDi852248.
    KEGGisce:YBL032W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77291 Genomic DNA. Translation: CAA54496.1 .
    Z35793 Genomic DNA. Translation: CAA84852.1 .
    AY692814 Genomic DNA. Translation: AAT92833.1 .
    BK006936 Genomic DNA. Translation: DAA07087.1 .
    PIRi S45766.
    RefSeqi NP_009521.1. NM_001178272.1.

    3D structure databases

    ProteinModelPortali P38199.
    SMRi P38199. Positions 43-226.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32665. 1153 interactions.
    DIPi DIP-3934N.
    IntActi P38199. 51 interactions.
    MINTi MINT-506819.
    STRINGi 4932.YBL032W.

    Proteomic databases

    MaxQBi P38199.
    PaxDbi P38199.
    PeptideAtlasi P38199.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBL032W ; YBL032W ; YBL032W .
    GeneIDi 852248.
    KEGGi sce:YBL032W.

    Organism-specific databases

    CYGDi YBL032w.
    SGDi S000000128. HEK2.

    Phylogenomic databases

    eggNOGi NOG294694.
    OMAi VANCINE.
    OrthoDBi EOG77HDRG.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28935-MONOMER.

    Miscellaneous databases

    NextBioi 970814.
    PROi P38199.

    Gene expression databases

    Genevestigatori P38199.

    Family and domain databases

    Gene3Di 3.30.1370.10. 3 hits.
    InterProi IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    [Graphical view ]
    Pfami PF00013. KH_1. 3 hits.
    PF13014. KH_3. 1 hit.
    [Graphical view ]
    SMARTi SM00322. KH. 3 hits.
    [Graphical view ]
    SUPFAMi SSF54791. SSF54791. 3 hits.
    PROSITEi PS50084. KH_TYPE_1. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of a 17.4 kb DNA segment of yeast chromosome II encompassing the ribosomal protein L19 as well as proteins with homologies to components of the hnRNP and snRNP complexes and to the human proliferation-associated p120 antigen."
      van Dyck L., Jonniaux J.-L., Barreiros T.D.M., Kleine K., Goffeau A.
      Yeast 10:1663-1673(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "KH domain-containing proteins of yeast: absence of a fragile X gene homologue."
      Currie J.R., Brown W.T.
      Am. J. Med. Genet. 84:272-276(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN.
    6. "The Khd1 protein, which has three KH RNA-binding motifs, is required for proper localization of ASH1 mRNA in yeast."
      Irie K., Tadauchi T., Takizawa P.A., Vale R.D., Matsumoto K., Herskowitz I.
      EMBO J. 21:1158-1167(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: RNA-BINDING, FUNCTION.
    7. "Yeast hnRNP K-like genes are involved in regulation of the telomeric position effect and telomere length."
      Denisenko O., Bomsztyk K.
      Mol. Cell. Biol. 22:286-297(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "The binding activity of yeast RNAs to yeast Hek2p and mammalian hnRNP K proteins, determined using the three-hybrid system."
      Paziewska A., Wyrwicz L.S., Ostrowski J.
      Cell. Mol. Biol. Lett. 10:227-235(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: RNA-BINDING.
    11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    12. "Local activation of yeast ASH1 mRNA translation through phosphorylation of Khd1p by the casein kinase Yck1p."
      Paquin N., Menade M., Poirier G., Donato D., Drouet E., Chartrand P.
      Mol. Cell 26:795-809(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, RNA-BINDING, PHOSPHORYLATION.
    13. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "Epistatic interaction between the K-homology domain protein HEK2 and SIR1 at HMR and telomeres in yeast."
      Denisenko O., Bomsztyk K.
      J. Mol. Biol. 375:1178-1187(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    15. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360 AND SER-362, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    16. "Distinct roles for Khd1p in the localization and expression of bud-localized mRNAs in yeast."
      Hasegawa Y., Irie K., Gerber A.P.
      RNA 14:2333-2347(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, RNA-BINDING.
    17. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    18. "Stability control of MTL1 mRNA by the RNA-binding protein Khd1p in yeast."
      Mauchi N., Ohtake Y., Irie K.
      Cell Struct. Funct. 35:95-105(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiHEK2_YEAST
    AccessioniPrimary (citable) accession number: P38199
    Secondary accession number(s): D6VPW7, E9P8X5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 15600 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3