Reviewed, UniProtKB/Swiss-Prot P38196 (FUI1_YEAST)

Names and origin

Protein names

Recommended name:
    Uridine permease

Gene names

Name:	FUI1
Ordered Locus Names:	YBL042C
ORF Names:	YBL0406

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	High-affinity transport of uridine.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the purine-cytosine permease family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Transmembrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transmembrane transport Ref.4 Inferred from mutant phenotype. Source: SGD uridine transport Ref.4 Inferred from direct assay. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Ref.4 Inferred from direct assay. Source: SGD
Molecular function	nucleobase transmembrane transporter activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct uridine transmembrane transporter activity Ref.4 Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P53278	1	EBI-7158,EBI-23314
AGP1	P25376	1	EBI-7158,EBI-2357
FMP45	Q07651	1	EBI-7158,EBI-2051056
HNM1	P19807	1	EBI-7158,EBI-8409
HXT7	P39004	1	EBI-7158,EBI-8790
MID2	P36027	1	EBI-7158,EBI-10901
MUP1	P50276	1	EBI-7158,EBI-11624
PDR12	Q02785	1	EBI-7158,EBI-13065
PMP2	P40975	1	EBI-7158,EBI-2043041
RFS1	P38234	1	EBI-7158,EBI-21445
SAC1	P32368	1	EBI-7158,EBI-16210
SPF1	P39986	1	EBI-7158,EBI-3128

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 639	639	Uridine permease		PRO_0000197923
Regions
	Topological domain	1 – 162	162	Cytoplasmic Potential
	Transmembrane	163 – 180	18	Potential
	Topological domain	181 – 200	20	Extracellular Potential
	Transmembrane	201 – 225	25	Potential
	Topological domain	226 – 259	34	Cytoplasmic Potential
	Transmembrane	260 – 276	17	Potential
	Topological domain	277 – 283	7	Extracellular Potential
	Transmembrane	284 – 305	22	Potential
	Topological domain	306 – 367	62	Cytoplasmic Potential
	Transmembrane	368 – 392	25	Potential
	Topological domain	393 – 416	24	Extracellular Potential
	Transmembrane	417 – 435	19	Potential
	Topological domain	436 – 460	25	Cytoplasmic Potential
	Transmembrane	461 – 477	17	Potential
	Topological domain	478 – 483	6	Extracellular Potential
	Transmembrane	484 – 507	24	Potential
	Topological domain	508 – 537	30	Cytoplasmic Potential
	Transmembrane	538 – 562	25	Potential
	Topological domain	563 – 572	10	Extracellular Potential
	Transmembrane	573 – 590	18	Potential
	Topological domain	591 – 639	49	Cytoplasmic Potential
Amino acid modifications
	Modified residue	4	1	Phosphoserine Ref.7
	Modified residue	54	1	Phosphothreonine Ref.6
	Modified residue	56	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P38196-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 757325DEF4100399	FASTA	639	72,165
	10 20 30 40 50 60 MPVSDSGFDN SSKTMKDDTI PTEDYEEITK ESEMGDATKI TSKIDANVIE KKDTDSENNI 70 80 90 100 110 120 TIAQDDEKVS WLQRVVEFFE VKNDSTDLAD HKPENPIRTF KDLQESLRST YLYNTDLRPV 130 140 150 160 170 180 EAKRRTWTWK QYIFFWISGS FNVNTWQISA TGLQLGLNWW QTWICIWVGY TFVAFFLILG 190 200 210 220 230 240 SKVGNNYHIS FPISSRVSFG IYFSIWIVIN RVVMACVWNS TLAYIGSQCV QLMLKAIFGT 250 260 270 280 290 300 NLNTRIKDTI KNPNLTNFEF MCFMVFWVAC LPFLWFPPDK LRHIFALKSA ITPFAAFGFL 310 320 330 340 350 360 IWTLCKAKGH LALGSLNDNG GAISKTVLAW SVIRAIMSAL DNFSTLILNA PDFTRFGKTY 370 380 390 400 410 420 KSSVYSQLIA LPVCYAIISL IGILSVSAAY TLYGVNYWSP LDILNRYLDN YTSGNRAGVF 430 440 450 460 470 480 LISFIFAFDQ LGANLSGNSI PAGTDLTALL PKFINIRRGS YICALISLAI CPWDLLSSSS 490 500 510 520 530 540 KFTTALAAYA VFLSAIAGVI SADYFIVRKG YVNIFHCYTD KPGSYYMYNK YGTNWRAVVA 550 560 570 580 590 600 YIFGIAPNFA GFLGSVGVSV PIGAMKVYYL NYFVGYLLAA LSYCILVYFY PIKGIPGDAK 610 620 630 ITDRKWLEEW VEVEEFGTER EAFEEYGGVS TGYEKIRYI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 22.4 kb DNA fragment from the left arm of yeast chromosome II reveals homologues to bacterial proline synthetase and murine alpha-adaptin, as well as a new permease and a DNA-binding protein." de Wergifosse P., Jacques B., Jonniaux J.-L., Purnelle B., Skala J., Goffeau A. Yeast 10:1489-1496(1994) [PubMed: 7871888] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The ORF YBL042 of Saccharomyces cerevisiae encodes a uridine permease." Wagner R., de Montigny J., de Wergifosse P., Souciet J.-L., Potier S. FEMS Microbiol. Lett. 159:69-75(1998) [PubMed: 9485596] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Nucleoside transporter proteins of Saccharomyces cerevisiae. Demonstration of a transporter (FUI1) with high uridine selectivity in plasma membranes and a transporter (FUN26) with broad nucleoside selectivity in intracellular membranes." Vickers M.F., Yao S.Y., Baldwin S.A., Young J.D., Cass C.E. J. Biol. Chem. 275:25931-25938(2000) [PubMed: 10827169] [Abstract] Cited for: CHARACTERIZATION.
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-54 AND SER-56, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X78214 Genomic DNA. Translation: CAA55059.1. Z35803 Genomic DNA. Translation: CAA84862.1.
PIR	S45776.
RefSeq	NP_009511.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1321N.
IntAct	P38196. 13 interactions.
STRING	P38196.
Protein family/group databases
TCDB	2.A.39.3.3. nucleobase:cation symporter-1 (NCS1) family.
Genome annotation databases
Ensembl	YBL042C; YBL042C; YBL042C; Saccharomyces cerevisiae. [Genome view]
GeneID	852238.
GenomeReviews	Gene locus YBL042C in contig Y13134_GR.
KEGG	sce:YBL042C.
NMPDR	fig|4932.3.peg.201.
Organism-specific databases
CYGD	YBL042c.
SGD	S000000138. FUI1.
Phylogenomic databases
HOGENOM	P38196.
OMA	YGTNWRA.
Gene expression databases
ArrayExpress	P38196.
Genevestigator	P38196.
GermOnline	YBL042C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001248. Cyt_pur_permease. IPR012681. NCS1. [Graphical view]
Pfam	PF02133. Transp_cyt_pur. 1 hit. [Graphical view]
TIGRFAMs	TIGR00800. ncs1. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	970784.

Entry information

Entry name

FUI1_YEAST

Accession

Primary (citable) accession number: P38196

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 3, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)