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Protein

Serine/threonine-protein phosphatase 4 regulatory subunit 2

Gene

PSY4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of the histone H2A phosphatase complex, which dephosphorylates H2AS128ph (gamma-H2A) that has been displaced from sites of DNA lesions in the double-stranded DNA break repair process. Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint.1 Publication

GO - Molecular functioni

  • protein phosphatase regulator activity Source: SGD

GO - Biological processi

  • negative regulation of DNA damage checkpoint Source: SGD
  • protein dephosphorylation Source: SGD
  • regulation of catalytic activity Source: GOC
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-28946-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 4 regulatory subunit 2
Short name:
PP4R2
Gene namesi
Name:PSY4
Synonyms:HTP3
Ordered Locus Names:YBL046W
ORF Names:YBL0402
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL046W.
SGDiS000000142. PSY4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
  • protein phosphatase 4 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 441441Serine/threonine-protein phosphatase 4 regulatory subunit 2PRO_0000202458Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei347 – 3471PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38193.

PTM databases

iPTMnetiP38193.

Interactioni

Subunit structurei

Regulatory subunit (R2) of the histone H2A phosphatase complex (HTP-C) consisting of PPH3, PSY2 and PSY4. Interacts with SPT4 and SPT5.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PPH3P323458EBI-21239,EBI-12759
PSY2P401648EBI-21239,EBI-29107

Protein-protein interaction databases

BioGridi32651. 36 interactions.
DIPiDIP-4295N.
IntActiP38193. 8 interactions.
MINTiMINT-553643.

Structurei

3D structure databases

ProteinModelPortaliP38193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi198 – 302105Asp-richAdd
BLAST

Sequence similaritiesi

Belongs to the PPP4R2 family.Curated

Phylogenomic databases

HOGENOMiHOG000115651.
InParanoidiP38193.
OMAiIVRICEL.
OrthoDBiEOG7G7M2G.

Family and domain databases

InterProiIPR015267. PPP4R2.
[Graphical view]
PANTHERiPTHR16487. PTHR16487. 1 hit.
PfamiPF09184. PPP4R2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38193-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTMLDDVD NNMMGIKSIS LYELLSDVVK QGDKTRLVTA GPEQVLPDLI
60 70 80 90 100
RHITETIPFD LFINLKNEMN DARNLVTRLN WLGKFLNDNF LQNHTFPFTI
110 120 130 140 150
LRICELCYDP FKYYKINELE KFVNALEKCC MVTSSWQVFD KTHGEKQEDD
160 170 180 190 200
KEKDINFIKN QEDVSLMKIP WMTENNTREL APFIREIDSI MSVNLGYDDE
210 220 230 240 250
DEEEGFFDGD EDREMGNKSK RNVLLKDENF MVEEYYEDDC GINDDNSDNK
260 270 280 290 300
GQNCQSDVTK NNSDDEDDDD NDDDYREDGA DEDDEDDDHM GSTDDDEDDD
310 320 330 340 350
EDRQAGESTK VQNFDKKNET PRKRKPTDLD NFEYDESPSF TNMDLTTPKK
360 370 380 390 400
YKHTATGRFS IIESPSSSLL NAMDGSNEIS SSQEEEKEDA HENHEGRSEG
410 420 430 440
LLPGDELVSP SMSSSQEDKM VAIAGITYRE NISSPLGKKS R
Length:441
Mass (Da):50,655
Last modified:October 1, 1994 - v1
Checksum:i74BCA72FCF2BD9A2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti374 – 3741D → V in AAT92597 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78214 Genomic DNA. Translation: CAA55049.1.
Z35807 Genomic DNA. Translation: CAA84866.1.
AY692578 Genomic DNA. Translation: AAT92597.1.
BK006936 Genomic DNA. Translation: DAA07072.1.
PIRiS42499.
RefSeqiNP_009507.1. NM_001178286.1.

Genome annotation databases

EnsemblFungiiYBL046W; YBL046W; YBL046W.
GeneIDi852234.
KEGGisce:YBL046W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78214 Genomic DNA. Translation: CAA55049.1.
Z35807 Genomic DNA. Translation: CAA84866.1.
AY692578 Genomic DNA. Translation: AAT92597.1.
BK006936 Genomic DNA. Translation: DAA07072.1.
PIRiS42499.
RefSeqiNP_009507.1. NM_001178286.1.

3D structure databases

ProteinModelPortaliP38193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32651. 36 interactions.
DIPiDIP-4295N.
IntActiP38193. 8 interactions.
MINTiMINT-553643.

PTM databases

iPTMnetiP38193.

Proteomic databases

MaxQBiP38193.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL046W; YBL046W; YBL046W.
GeneIDi852234.
KEGGisce:YBL046W.

Organism-specific databases

EuPathDBiFungiDB:YBL046W.
SGDiS000000142. PSY4.

Phylogenomic databases

HOGENOMiHOG000115651.
InParanoidiP38193.
OMAiIVRICEL.
OrthoDBiEOG7G7M2G.

Enzyme and pathway databases

BioCyciYEAST:G3O-28946-MONOMER.

Miscellaneous databases

PROiP38193.

Family and domain databases

InterProiIPR015267. PPP4R2.
[Graphical view]
PANTHERiPTHR16487. PTHR16487. 1 hit.
PfamiPF09184. PPP4R2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 22.4 kb DNA fragment from the left arm of yeast chromosome II reveals homologues to bacterial proline synthetase and murine alpha-adaptin, as well as a new permease and a DNA-binding protein."
    de Wergifosse P., Jacques B., Jonniaux J.-L., Purnelle B., Skala J., Goffeau A.
    Yeast 10:1489-1496(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A novel, evolutionarily conserved protein phosphatase complex involved in cisplatin sensitivity."
    Gingras A.-C., Caballero M., Zarske M., Sanchez A., Hazbun T.R., Fields S., Sonenberg N., Hafen E., Raught B., Aebersold R.
    Mol. Cell. Proteomics 4:1725-1740(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN HTP-C COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH SPT4 AND SPT5.
  8. Cited for: FUNCTION, IDENTIFICATION IN HTP-C COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-347, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPP4R2_YEAST
AccessioniPrimary (citable) accession number: P38193
Secondary accession number(s): D6VPV2, Q6B302
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 8, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1010 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.