P38193 (PP4R2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 4 regulatory subunit 2
Short name=PP4R2

Gene names

Name:	PSY4
Synonyms:	HTP3
Ordered Locus Names:	YBL046W
ORF Names:	YBL0402

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory subunit of the histone H2A phosphatase complex, which dephosphorylates H2AS128ph (gamma-H2A) that has been displaced from sites of DNA lesions in the double-stranded DNA break repair process. Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint. Ref.9
Subunit structure	Regulatory subunit (R2) of the histone H2A phosphatase complex (HTP-C) consisting of PPH3, PSY2 and PSY4. Interacts with SPT4 and SPT5. Ref.8
Subcellular location	Nucleus Ref.5.
Miscellaneous	Present with 1010 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the PPP4R2 family.

Ontologies

Keywords
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of DNA damage checkpoint Inferred from mutant phenotype Ref.9. Source: SGD protein dephosphorylation Inferred from physical interaction. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay Ref.5. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein phosphatase regulator activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PPH3	P32345	9	EBI-21239,EBI-12759
PSY2	P40164	8	EBI-21239,EBI-29107

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 441	441	Serine/threonine-protein phosphatase 4 regulatory subunit 2		PRO_0000202458
Regions
	Compositional bias	198 – 302	105	Asp-rich
Amino acid modifications
	Modified residue	347	1	Phosphothreonine Ref.10
	Modified residue	414	1	Phosphoserine Ref.11
	Modified residue	415	1	Phosphoserine Ref.11
	Modified residue	434	1	Phosphoserine Ref.7 Ref.10
Experimental info
	Sequence conflict	374	1	D → V in AAT92597. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P38193 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 74BCA72FCF2BD9A2	FASTA	441	50,655
	10 20 30 40 50 60 MSSTMLDDVD NNMMGIKSIS LYELLSDVVK QGDKTRLVTA GPEQVLPDLI RHITETIPFD 70 80 90 100 110 120 LFINLKNEMN DARNLVTRLN WLGKFLNDNF LQNHTFPFTI LRICELCYDP FKYYKINELE 130 140 150 160 170 180 KFVNALEKCC MVTSSWQVFD KTHGEKQEDD KEKDINFIKN QEDVSLMKIP WMTENNTREL 190 200 210 220 230 240 APFIREIDSI MSVNLGYDDE DEEEGFFDGD EDREMGNKSK RNVLLKDENF MVEEYYEDDC 250 260 270 280 290 300 GINDDNSDNK GQNCQSDVTK NNSDDEDDDD NDDDYREDGA DEDDEDDDHM GSTDDDEDDD 310 320 330 340 350 360 EDRQAGESTK VQNFDKKNET PRKRKPTDLD NFEYDESPSF TNMDLTTPKK YKHTATGRFS 370 380 390 400 410 420 IIESPSSSLL NAMDGSNEIS SSQEEEKEDA HENHEGRSEG LLPGDELVSP SMSSSQEDKM 430 440 VAIAGITYRE NISSPLGKKS R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 22.4 kb DNA fragment from the left arm of yeast chromosome II reveals homologues to bacterial proline synthetase and murine alpha-adaptin, as well as a new permease and a DNA-binding protein." de Wergifosse P., Jacques B., Jonniaux J.-L., Purnelle B., Skala J., Goffeau A. Yeast 10:1489-1496(1994) [PubMed: 7871888] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-434, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"A novel, evolutionarily conserved protein phosphatase complex involved in cisplatin sensitivity." Gingras A.-C., Caballero M., Zarske M., Sanchez A., Hazbun T.R., Fields S., Sonenberg N., Hafen E., Raught B., Aebersold R. Mol. Cell. Proteomics 4:1725-1740(2005) [PubMed: 16085932] [Abstract] Cited for: IDENTIFICATION IN HTP-C COMPLEX, MASS SPECTROMETRY, INTERACTION WITH SPT4 AND SPT5.
[9]	"A phosphatase complex that dephosphorylates gamma-H2AX regulates DNA damage checkpoint recovery." Keogh M.-C., Kim J.-A., Downey M., Fillingham J., Chowdhury D., Harrison J.C., Onishi M., Datta N., Galicia S., Emili A., Lieberman J., Shen X., Buratowski S., Haber J.E., Durocher D., Greenblatt J.F., Krogan N.J. Nature 439:497-501(2006) [PubMed: 16299494] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN HTP-C COMPLEX, MASS SPECTROMETRY.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-347 AND SER-434, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-414 AND SER-415, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78214 Genomic DNA. Translation: CAA55049.1. Z35807 Genomic DNA. Translation: CAA84866.1. AY692578 Genomic DNA. Translation: AAT92597.1. BK006936 Genomic DNA. Translation: DAA07072.1.
PIR	S42499.
RefSeq	NP_009507.1. NM_001178286.1.
3D structure databases
ProteinModelPortal	P38193.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4295N.
IntAct	P38193. 10 interactions.
MINT	MINT-553643.
STRING	P38193.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL046W; YBL046W; YBL046W.
GeneID	852234.
KEGG	sce:YBL046W.
NMPDR	fig|4932.3.peg.197.
Organism-specific databases
CYGD	YBL046w.
SGD	S000000142. PSY4.
Phylogenomic databases
eggNOG	fuNOG11701.
OMA	HITETIP.
OrthoDB	EOG4RJKB7.
Gene expression databases
ArrayExpress	P38193.
Genevestigator	P38193.
GermOnline	YBL046W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015267. PPP4R2. [Graphical view]
Pfam	PF09184. PPP4R2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970772.

Entry information

Entry name

PP4R2_YEAST

Accession

Primary (citable) accession number: P38193
Secondary accession number(s): D6VPV2, Q6B302

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 14, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program