##gff-version 3
P38182	UniProtKB	Chain	1	116	.	.	.	ID=PRO_0000017242;Note=Autophagy-related protein 8	
P38182	UniProtKB	Propeptide	117	117	.	.	.	ID=PRO_0000017243;Note=Removed in mature form;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11038174;Dbxref=PMID:11038174	
P38182	UniProtKB	Site	116	117	.	.	.	Note=Cleavage%3B by ATG4;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11038174;Dbxref=PMID:11038174	
P38182	UniProtKB	Lipidation	116	116	.	.	.	Note=Phosphatidylethanolamine amidated glycine;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11100732;Dbxref=PMID:11100732	
P38182	UniProtKB	Mutagenesis	26	26	.	.	.	Note=No effect on autophagic activity. K->P;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20428927;Dbxref=PMID:20428927	
P38182	UniProtKB	Mutagenesis	28	28	.	.	.	Note=Impairs ATG19-binding and autophagic activity. R->D;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:19021777,ECO:0000269|PubMed:19398890;Dbxref=PMID:19021777,PMID:19398890	
P38182	UniProtKB	Mutagenesis	48	48	.	.	.	Note=Impairs autophagic activity. K->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19398890;Dbxref=PMID:19398890	
P38182	UniProtKB	Mutagenesis	49	49	.	.	.	Note=Impairs ATG19-binding%2C PE-conjugation%2C and autophagic activity. Y->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:16680092,ECO:0000269|PubMed:19398890;Dbxref=PMID:16680092,PMID:19398890	
P38182	UniProtKB	Mutagenesis	50	50	.	.	.	Note=Impairs ATG19-binding and autophagic activity. L->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:16680092,ECO:0000269|PubMed:19398890;Dbxref=PMID:16680092,PMID:19398890	
P38182	UniProtKB	Mutagenesis	52	52	.	.	.	Note=Significantly decreases ATG19-binding and autophagic activity. P->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19021777;Dbxref=PMID:19021777	
P38182	UniProtKB	Mutagenesis	55	55	.	.	.	Note=Impairs autophagic activity. L->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19398890;Dbxref=PMID:19398890	
P38182	UniProtKB	Mutagenesis	67	67	.	.	.	Note=Significantly decreases ATG19-binding and autophagic activity. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19021777;Dbxref=PMID:19021777	
P38182	UniProtKB	Mutagenesis	77	77	.	.	.	Note=Impairs interaction with ATG4%2C cleavage by ATG4%2C PAS localization%2C and autophagic activity%3B when associated with A-79. F->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16680092;Dbxref=PMID:16680092	
P38182	UniProtKB	Mutagenesis	79	79	.	.	.	Note=Impairs interaction with ATG4%2C cleavage by ATG4%2C PAS localization%2C and autophagic activity%3B when associated with A-77. F->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16680092;Dbxref=PMID:16680092	
P38182	UniProtKB	Mutagenesis	116	116	.	.	.	Note=No cleaving of the C-terminal R-117 by ATG4. Defect in Cvt pathway and autophagy. G->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11038174;Dbxref=PMID:11038174	
P38182	UniProtKB	Mutagenesis	117	117	.	.	.	Note=Normal processing after G-116 by ATG4. R->M;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11038174;Dbxref=PMID:11038174	
P38182	UniProtKB	Helix	5	8	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6WY6	
P38182	UniProtKB	Helix	11	24	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6WY6	
P38182	UniProtKB	Beta strand	28	35	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6WY6	
P38182	UniProtKB	Beta strand	37	39	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:5YEC	
P38182	UniProtKB	Beta strand	48	52	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6WY6	
P38182	UniProtKB	Helix	57	67	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6WY6	
P38182	UniProtKB	Beta strand	77	80	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6WY6	
P38182	UniProtKB	Beta strand	87	90	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2KWC	
P38182	UniProtKB	Helix	91	98	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6WY6	
P38182	UniProtKB	Beta strand	105	112	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6WY6