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P38182

- ATG8_YEAST

UniProt

P38182 - ATG8_YEAST

Protein

Autophagy-related protein 8

Gene

ATG8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Ubiquitin-like modifier involved in cytoplasm to vacuole transport (Cvt) vesicles and autophagosomes formation. With ATG4, mediates the delivery of the vesicles and autophagosomes to the vacuole via the microtubule cytoskeleton. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Participates also in membrane fusion events that take place in the early secretory pathway. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy. Moreover not only conjugation, but also subsequent ATG8-PE deconjugation is an important step required to facilitate multiple events during macroautophagy, and especially for efficient autophagosome biogenesis, the assembly of Atg9-containing tubulovesicular clusters into phagophores/autophagosomes, and for the disassembly of PAS-associated ATG components. Plays also a role in regulation of filamentous growth.20 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei116 – 1172Cleavage; by ATG4

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. protein tag Source: SGD

    GO - Biological processi

    1. autophagic vacuole assembly Source: UniProtKB
    2. autophagy Source: UniProtKB
    3. cellular protein complex localization Source: SGD
    4. CVT pathway Source: SGD
    5. ER to Golgi vesicle-mediated transport Source: SGD
    6. late nucleophagy Source: SGD
    7. membrane fusion Source: SGD
    8. mitochondrion degradation Source: SGD
    9. piecemeal microautophagy of nucleus Source: SGD
    10. protein targeting to vacuole involved in autophagy Source: SGD

    Keywords - Biological processi

    Autophagy, Protein transport, Transport, Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28969-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Autophagy-related protein 8
    Alternative name(s):
    Autophagy-related ubiquitin-like modifier ATG8
    Cytoplasm to vacuole targeting protein 5
    Gene namesi
    Name:ATG8
    Synonyms:APG8, AUT7, CVT5
    Ordered Locus Names:YBL078C
    ORF Names:YBL0732
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBL078c.
    SGDiS000000174. ATG8.

    Subcellular locationi

    Cytoplasmic vesiclecvt vesicle membrane; Lipid-anchor. Cytoplasmic vesicleautophagosome membrane; Lipid-anchor. Vacuole membrane; Lipid-anchor
    Note: Membrane-associated through a lipid anchor. This association needs the 2 ubiquitin-like systems required for cytoplasm to vacuole transport and autophagy. ATG18 and ATG21 facilitate the recruitment of ATG8-PE to the site of autophagosome formation and protect it from premature cleavage by ATG4. Localizes to both the isolation membrane (IM) and the vacuole-isolation membrane contact site (VICS) during IM expansion. The IM is a membrane sac generated from the pre-autophagosomal structure that ultimately expands to become a mature autophagosome. Upon starvation, is also recruited to into unique membrane structures near SEC13-containing ER exit sites which lack components of the Golgi apparatus and the endosomes, and which were called a compartments for unconventional protein secretion (CUPS).

    GO - Cellular componenti

    1. autophagic vacuole Source: UniProtKB
    2. autophagic vacuole membrane Source: UniProtKB-SubCell
    3. Cvt vesicle membrane Source: UniProtKB-SubCell
    4. cytoplasm Source: UniProtKB
    5. cytosol Source: UniProtKB
    6. extrinsic component of membrane Source: SGD
    7. fungal-type vacuole membrane Source: SGD
    8. pre-autophagosomal structure Source: SGD

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Membrane, Vacuole

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi26 – 261K → P: No effect on autophagic activity. 1 Publication
    Mutagenesisi28 – 281R → D: Impairs ATG19-binding and autophagic activity. 2 Publications
    Mutagenesisi48 – 481K → E: Impairs autophagic activity. 1 Publication
    Mutagenesisi49 – 491Y → A: Impairs ATG19-binding, PE-conjugation, and autophagic activity. 2 Publications
    Mutagenesisi50 – 501L → A: Impairs ATG19-binding and autophagic activity. 2 Publications
    Mutagenesisi52 – 521P → A: Significantly decreases ATG19-binding and autophagic activity. 1 Publication
    Mutagenesisi55 – 551L → A: Impairs autophagic activity. 1 Publication
    Mutagenesisi67 – 671R → A: Significantly decreases ATG19-binding and autophagic activity. 1 Publication
    Mutagenesisi77 – 771F → A: Impairs interaction with ATG4, cleavage by ATG4, PAS localization, and autophagic activity; when associated with A-79. 1 Publication
    Mutagenesisi79 – 791F → A: Impairs interaction with ATG4, cleavage by ATG4, PAS localization, and autophagic activity; when associated with A-77. 1 Publication
    Mutagenesisi116 – 1161G → A: No cleaving of the C-terminal R-117 by ATG4. Defect in Cvt pathway and autophagy. 1 Publication
    Mutagenesisi117 – 1171R → M: Normal processing after G-116 by ATG4. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 116116Autophagy-related protein 8PRO_0000017242Add
    BLAST
    Propeptidei117 – 1171Removed in mature formPRO_0000017243

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi116 – 1161Phosphatidylethanolamine amidated glycine1 Publication

    Post-translational modificationi

    The C-terminal Arg-117 residue of ATG8 is removed by ATG4 to expose Gly-116 at the C-terminus. This Gly-116 forms then a thioester bond with the 'Cys-507' of ATG7 (E1-like activating enzyme) before being transferred to the 'Cys-234' of ATG3 (the specific E2 conjugating enzyme), in order to be finally amidated with phosphatidylethanolamine. This lipid modification anchors ATG8 to membranes and can be reversed by ATG4, releasing soluble ATG8 (PubMed:11100732).1 Publication

    Keywords - PTMi

    Lipoprotein

    Proteomic databases

    MaxQBiP38182.
    PaxDbiP38182.
    PeptideAtlasiP38182.

    Miscellaneous databases

    PMAP-CutDBP38182.

    Expressioni

    Inductioni

    Up-regulated upon starvation conditions. Expression is under the control of UME6 which acts along with a histone deacetylase complex including SIN3 and RPD3 to regulate negatively ATG8 levels and subsequent autophagic activity.2 Publications

    Gene expression databases

    GenevestigatoriP38182.

    Interactioni

    Subunit structurei

    Conjugation to phosphatidylethanolamine (PE) leads to homodimerization. Interacts with ATG1, ATG3, ATG4, ATG7, ATG12, ATG32, ATG34 and the C-terminal 10 residues domain of ATG19. Interacts also with the endoplasmic reticulum to Golgi v-SNARE protein BET1 and the vacuolar v-SNARE protein NYV1. Interacts with the UBX domain-containing protein SHP1.20 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ATG1P531043EBI-2684,EBI-2657
    ATG3P403443EBI-2684,EBI-3381
    ATG4P538673EBI-2684,EBI-29160
    ATG7P388623EBI-2684,EBI-2677
    BET1P228042EBI-2684,EBI-3550
    NBR1Q145967EBI-2684,EBI-742698From a different organism.
    SQSTM1Q135013EBI-2684,EBI-307104From a different organism.

    Protein-protein interaction databases

    BioGridi32625. 116 interactions.
    DIPiDIP-1197N.
    IntActiP38182. 15 interactions.
    MINTiMINT-391771.
    STRINGi4932.YBL078C.

    Structurei

    Secondary structure

    1
    117
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 83
    Helixi11 – 2414
    Beta strandi26 – 3510
    Beta strandi37 – 393
    Beta strandi48 – 525
    Helixi57 – 6711
    Beta strandi76 – 805
    Beta strandi87 – 904
    Helixi91 – 988
    Beta strandi105 – 1117

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2KQ7NMR-A1-117[»]
    2KWCNMR-A1-116[»]
    2LI5NMR-A1-116[»]
    2ZPNX-ray2.70A/B/C/D1-116[»]
    3RUIX-ray1.91B1-116[»]
    3VH3X-ray2.00B1-116[»]
    3VH4X-ray2.65B1-116[»]
    3VXWX-ray3.00A1-116[»]
    ProteinModelPortaliP38182.
    SMRiP38182. Positions 4-116.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38182.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATG8 family.Curated

    Phylogenomic databases

    eggNOGiNOG249730.
    GeneTreeiENSGT00390000012937.
    HOGENOMiHOG000232034.
    KOiK08341.
    OMAiHALEHRC.
    OrthoDBiEOG7712KX.

    Family and domain databases

    InterProiIPR004241. Atg8_like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PANTHERiPTHR10969. PTHR10969. 1 hit.
    PfamiPF02991. Atg8. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38182-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSTFKSEYP FEKRKAESER IADRFKNRIP VICEKAEKSD IPEIDKRKYL    50
    VPADLTVGQF VYVIRKRIML PPEKAIFIFV NDTLPPTAAL MSAIYQEHKD 100
    KDGFLYVTYS GENTFGR 117
    Length:117
    Mass (Da):13,627
    Last modified:October 1, 1994 - v1
    Checksum:i1246CEFF5A038819
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79489 Genomic DNA. Translation: CAA56032.1.
    Z35839 Genomic DNA. Translation: CAA84899.1.
    AY692870 Genomic DNA. Translation: AAT92889.1.
    BK006936 Genomic DNA. Translation: DAA07045.1.
    PIRiS45432.
    RefSeqiNP_009475.1. NM_001178318.1.

    Genome annotation databases

    EnsemblFungiiYBL078C; YBL078C; YBL078C.
    GeneIDi852200.
    KEGGisce:YBL078C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79489 Genomic DNA. Translation: CAA56032.1 .
    Z35839 Genomic DNA. Translation: CAA84899.1 .
    AY692870 Genomic DNA. Translation: AAT92889.1 .
    BK006936 Genomic DNA. Translation: DAA07045.1 .
    PIRi S45432.
    RefSeqi NP_009475.1. NM_001178318.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2KQ7 NMR - A 1-117 [» ]
    2KWC NMR - A 1-116 [» ]
    2LI5 NMR - A 1-116 [» ]
    2ZPN X-ray 2.70 A/B/C/D 1-116 [» ]
    3RUI X-ray 1.91 B 1-116 [» ]
    3VH3 X-ray 2.00 B 1-116 [» ]
    3VH4 X-ray 2.65 B 1-116 [» ]
    3VXW X-ray 3.00 A 1-116 [» ]
    ProteinModelPortali P38182.
    SMRi P38182. Positions 4-116.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32625. 116 interactions.
    DIPi DIP-1197N.
    IntActi P38182. 15 interactions.
    MINTi MINT-391771.
    STRINGi 4932.YBL078C.

    Proteomic databases

    MaxQBi P38182.
    PaxDbi P38182.
    PeptideAtlasi P38182.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBL078C ; YBL078C ; YBL078C .
    GeneIDi 852200.
    KEGGi sce:YBL078C.

    Organism-specific databases

    CYGDi YBL078c.
    SGDi S000000174. ATG8.

    Phylogenomic databases

    eggNOGi NOG249730.
    GeneTreei ENSGT00390000012937.
    HOGENOMi HOG000232034.
    KOi K08341.
    OMAi HALEHRC.
    OrthoDBi EOG7712KX.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28969-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P38182.
    NextBioi 970694.
    PMAP-CutDB P38182.
    PROi P38182.

    Gene expression databases

    Genevestigatori P38182.

    Family and domain databases

    InterProi IPR004241. Atg8_like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    PANTHERi PTHR10969. PTHR10969. 1 hit.
    Pfami PF02991. Atg8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
      Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
      Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae."
      Tsukada M., Ohsumi Y.
      FEBS Lett. 333:169-174(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway."
      Harding T.M., Morano K.A., Scott S.V., Klionsky D.J.
      J. Cell Biol. 131:591-602(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Aut2p and Aut7p, two novel microtubule-associated proteins are essential for delivery of autophagic vesicles to the vacuole."
      Lang T., Schaeffeler E., Bernreuther D., Bredschneider M., Wolf D.H., Thumm M.
      EMBO J. 17:3597-3607(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ATG4, SUBCELLULAR LOCATION.
    8. "Formation process of autophagosome is traced with Apg8/Aut7p in yeast."
      Kirisako T., Baba M., Ishihara N., Miyazawa K., Ohsumi M., Yoshimori T., Noda T., Ohsumi Y.
      J. Cell Biol. 147:435-446(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "The itinerary of a vesicle component, Aut7p/Cvt5p, terminates in the yeast vacuole via the autophagy/Cvt pathways."
      Huang W.-P., Scott S.V., Kim J., Klionsky D.J.
      J. Biol. Chem. 275:5845-5851(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    10. "Aut7p, a soluble autophagic factor, participates in multiple membrane trafficking processes."
      Legesse-Miller A., Sagiv Y., Glozman R., Elazar Z.
      J. Biol. Chem. 275:32966-32973(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH BET1 AND NYV1.
    11. "The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathway."
      Kirisako T., Ichimura Y., Okada H., Kabeya Y., Mizushima N., Yoshimori T., Ohsumi M., Takao T., Noda T., Ohsumi Y.
      J. Cell Biol. 151:263-276(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CLEAVAGE BY ATG4, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-116 AND ARG-117.
    12. Cited for: FUNCTION, INTERACTION WITH ATG3 AND ATG7, LIPIDATION AT GLY-116.
    13. "The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation."
      Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y.
      EMBO J. 20:5971-5981(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    14. "The C-terminal region of an Apg7p/Cvt2p is required for homodimerization and is essential for its E1 activity and E1-E2 complex formation."
      Komatsu M., Tanida I., Ueno T., Ohsumi M., Ohsumi Y., Kominami E.
      J. Biol. Chem. 276:9846-9854(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG7.
    15. "Membrane recruitment of Aut7p in the autophagy and cytoplasm to vacuole targeting pathways requires Aut1p, Aut2p, and the autophagy conjugation complex."
      Kim J., Huang W.-P., Klionsky D.J.
      J. Cell Biol. 152:51-64(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CLEAVAGE BY ATG4, SUBCELLULAR LOCATION.
    16. "Mechanism of cargo selection in the cytoplasm to vacuole targeting pathway."
      Shintani T., Huang W.-P., Stromhaug P.E., Klionsky D.J.
      Dev. Cell 3:825-837(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG19.
    17. "Convergence of multiple autophagy and cytoplasm to vacuole targeting components to a perivacuolar membrane compartment prior to de novo vesicle formation."
      Kim J., Huang W.-P., Stromhaug P.E., Klionsky D.J.
      J. Biol. Chem. 277:763-773(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ATG12 AND ATG19.
    18. Cited for: NOMENCLATURE.
    19. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    20. "Atg21 is required for effective recruitment of Atg8 to the preautophagosomal structure during the Cvt pathway."
      Meiling-Wesse K., Barth H., Voss C., Eskelinen E.-L., Epple U.D., Thumm M.
      J. Biol. Chem. 279:37741-37750(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CONJUGATION TO PHOSPHATIDYLETHANOLAMINE, SUBCELLULAR LOCATION.
    21. "In vivo and in vitro reconstitution of atg8 conjugation essential for autophagy."
      Ichimura Y., Imamura Y., Emoto K., Umeda M., Noda T., Ohsumi Y.
      J. Biol. Chem. 279:40584-40592(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CONJUGATION TO PHOSPHATIDYLETHANOLAMINE.
    22. "Autophagy-related protein 8 (Atg8) family interacting motif in Atg3 mediates the Atg3-Atg8 interaction and is crucial for the cytoplasm-to-vacuole targeting pathway."
      Yamaguchi M., Noda N.N., Nakatogawa H., Kumeta H., Ohsumi Y., Inagaki F.
      J. Biol. Chem. 285:29599-29607(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG3, CONJUGATION TO PHOSPHATIDYLETHANOLAMINE.
    23. "Atg21 is a phosphoinositide binding protein required for efficient lipidation and localization of Atg8 during uptake of aminopeptidase I by selective autophagy."
      Stromhaug P.E., Reggiori F., Guan J., Wang C.-W., Klionsky D.J.
      Mol. Biol. Cell 15:3553-3566(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    24. "Interrelationships among Atg proteins during autophagy in Saccharomyces cerevisiae."
      Suzuki K., Noda T., Ohsumi Y.
      Yeast 21:1057-1065(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    25. "Trs85 (Gsg1), a component of the TRAPP complexes, is required for the organization of the preautophagosomal structure during selective autophagy via the Cvt pathway."
      Meiling-Wesse K., Epple U.D., Krick R., Barth H., Appelles A., Voss C., Eskelinen E.L., Thumm M.
      J. Biol. Chem. 280:33669-33678(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    26. "Atg11 links cargo to the vesicle-forming machinery in the cytoplasm to vacuole targeting pathway."
      Yorimitsu T., Klionsky D.J.
      Mol. Biol. Cell 16:1593-1605(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    27. "The actin cytoskeleton is required for selective types of autophagy, but not nonspecific autophagy, in the yeast Saccharomyces cerevisiae."
      Reggiori F., Monastyrska I., Shintani T., Klionsky D.J.
      Mol. Biol. Cell 16:5843-5856(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    28. "Two newly identified sites in the ubiquitin-like protein Atg8 are essential for autophagy."
      Amar N., Lustig G., Ichimura Y., Ohsumi Y., Elazar Z.
      EMBO Rep. 7:635-642(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATG4, CLEAVAGE BY ATG4, MUTAGENESIS OF TYR-49; LEU-50; PHE-77 AND PHE-79.
    29. "Autophagy counterbalances endoplasmic reticulum expansion during the unfolded protein response."
      Bernales S., McDonald K.L., Walter P.
      PLoS Biol. 4:E423-E423(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    30. "The role of autophagy in mitochondria maintenance: characterization of mitochondrial functions in autophagy-deficient S. cerevisiae strains."
      Zhang Y., Qi H., Taylor R., Xu W., Liu L.F., Jin S.
      Autophagy 3:337-346(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    31. "Overexpression of autophagy-related genes inhibits yeast filamentous growth."
      Ma J., Jin R., Dobry C.J., Lawson S.K., Kumar A.
      Autophagy 3:604-609(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    32. "Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion."
      Nakatogawa H., Ichimura Y., Ohsumi Y.
      Cell 130:165-178(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CONJUGATION TO PHOSPHATIDYLETHANOLAMINE, FUNCTION OF THE ATG8-PE CONJUGATE, SUBUNIT.
    33. "Hierarchy of Atg proteins in pre-autophagosomal structure organization."
      Suzuki K., Kubota Y., Sekito T., Ohsumi Y.
      Genes Cells 12:209-218(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    34. "Protein kinase A and Sch9 cooperatively regulate induction of autophagy in Saccharomyces cerevisiae."
      Yorimitsu T., Zaman S., Broach J.R., Klionsky D.J.
      Mol. Biol. Cell 18:4180-4189(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CONJUGATION TO PHOSPHATIDYLETHANOLAMINE.
    35. "Quantitative analysis of autophagy-related protein stoichiometry by fluorescence microscopy."
      Geng J., Baba M., Nair U., Klionsky D.J.
      J. Cell Biol. 182:129-140(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    36. "In vivo reconstitution of autophagy in Saccharomyces cerevisiae."
      Cao Y., Cheong H., Song H., Klionsky D.J.
      J. Cell Biol. 182:703-713(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: CONJUGATION TO PHOSPHATIDYLETHANOLAMINE.
    37. "The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae."
      Cheong H., Nair U., Geng J., Klionsky D.J.
      Mol. Biol. Cell 19:668-681(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    38. "Atg8 controls phagophore expansion during autophagosome formation."
      Xie Z., Nair U., Klionsky D.J.
      Mol. Biol. Cell 19:3290-3298(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    39. "Indirect estimation of the area density of Atg8 on the phagophore."
      Xie Z., Nair U., Geng J., Szefler M.B., Rothman E.D., Klionsky D.J.
      Autophagy 5:217-220(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    40. "Mutation at the cargo-receptor binding site of Atg8 also affects its general autophagy regulation function."
      Ho K.H., Chang H.E., Huang W.P.
      Autophagy 5:461-471(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ATG19, MUTAGENESIS OF ARG-28; LYS-48; TYR-49; LEU-50 AND LEU-55.
    41. "Mitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy."
      Okamoto K., Kondo-Okamoto N., Ohsumi Y.
      Dev. Cell 17:87-97(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG32.
    42. "The amino-terminal region of Atg3 is essential for association with phosphatidylethanolamine in Atg8 lipidation."
      Hanada T., Satomi Y., Takao T., Ohsumi Y.
      FEBS Lett. 583:1078-1083(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG3, CONJUGATION TO PHOSPHATIDYLETHANOLAMINE.
    43. "Accumulation of non-superoxide anion reactive oxygen species mediates nitrogen-limited alcoholic fermentation by Saccharomyces cerevisiae."
      Mendes-Ferreira A., Sampaio-Marques B., Barbosa C., Rodrigues F., Costa V., Mendes-Faia A., Ludovico P., Leao C.
      Appl. Environ. Microbiol. 76:7918-7924(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    44. "Roles of the lipid-binding motifs of Atg18 and Atg21 in the cytoplasm to vacuole targeting pathway and autophagy."
      Nair U., Cao Y., Xie Z., Klionsky D.J.
      J. Biol. Chem. 285:11476-11488(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    45. "Selective transport of alpha-mannosidase by autophagic pathways: identification of a novel receptor, Atg34p."
      Suzuki K., Kondo C., Morimoto M., Ohsumi Y.
      J. Biol. Chem. 285:30019-30025(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG34.
    46. "The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy."
      Yen W.L., Shintani T., Nair U., Cao Y., Richardson B.C., Li Z., Hughson F.M., Baba M., Klionsky D.J.
      J. Cell Biol. 188:101-114(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    47. "Cdc48/p97 and Shp1/p47 regulate autophagosome biogenesis in concert with ubiquitin-like Atg8."
      Krick R., Bremer S., Welter E., Schlotterhose P., Muehe Y., Eskelinen E.L., Thumm M.
      J. Cell Biol. 190:965-973(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SHP1.
    48. "An Atg9-containing compartment that functions in the early steps of autophagosome biogenesis."
      Mari M., Griffith J., Rieter E., Krishnappa L., Klionsky D.J., Reggiori F.
      J. Cell Biol. 190:1005-1022(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    49. "Biogenesis of a novel compartment for autophagosome-mediated unconventional protein secretion."
      Bruns C., McCaffery J.M., Curwin A.J., Duran J.M., Malhotra V.
      J. Cell Biol. 195:979-992(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    50. "Mitophagy in yeast is independent of mitochondrial fission and requires the stress response gene WHI2."
      Mendl N., Occhipinti A., Muller M., Wild P., Dikic I., Reichert A.S.
      J. Cell Sci. 124:1339-1350(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    51. "Atg4 recycles inappropriately lipidated Atg8 to promote autophagosome biogenesis."
      Nakatogawa H., Ishii J., Asai E., Ohsumi Y.
      Autophagy 8:177-186(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ATG8-PE DECONJUGATION.
    52. "A role for Atg8-PE deconjugation in autophagosome biogenesis."
      Nair U., Yen W.L., Mari M., Cao Y., Xie Z., Baba M., Reggiori F., Klionsky D.J.
      Autophagy 8:780-793(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF ATG8-PE DECONJUGATION.
    53. Cited for: ATG8-PE DECONJUGATION.
    54. Cited for: INTERACTION WITH ATG1.
    55. "Mechanism and functions of membrane binding by the Atg5-Atg12/Atg16 complex during autophagosome formation."
      Romanov J., Walczak M., Ibiricu I., Schuchner S., Ogris E., Kraft C., Martens S.
      EMBO J. 31:4304-4317(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: CONJUGATION TO PHOSPHATIDYLETHANOLAMINE.
    56. "A late form of nucleophagy in Saccharomyces cerevisiae."
      Mijaljica D., Prescott M., Devenish R.J.
      PLoS ONE 7:E40013-E40013(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    57. "The autophagy-related protein kinase Atg1 interacts with the ubiquitin-like protein Atg8 via the Atg8 family interacting motif to facilitate autophagosome formation."
      Nakatogawa H., Ohbayashi S., Sakoh-Nakatogawa M., Kakuta S., Suzuki S.W., Kirisako H., Kondo-Kakuta C., Noda N.N., Yamamoto H., Ohsumi Y.
      J. Biol. Chem. 287:28503-28507(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG1.
    58. Cited for: INDUCTION.
    59. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    60. "Function and molecular mechanism of acetylation in autophagy regulation."
      Yi C., Ma M., Ran L., Zheng J., Tong J., Zhu J., Ma C., Sun Y., Zhang S., Feng W., Zhu L., Le Y., Gong X., Yan X., Hong B., Jiang F.J., Xie Z., Miao D., Deng H., Yu L.
      Science 336:474-477(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG3, CONJUGATION TO PHOSPHATIDYLETHANOLAMINE.
    61. "Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae."
      Suzuki K., Akioka M., Kondo-Kakuta C., Yamamoto H., Ohsumi Y.
      J. Cell Sci. 126:2534-2544(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    62. "Structural basis of target recognition by Atg8/LC3 during selective autophagy."
      Noda N.N., Kumeta H., Nakatogawa H., Satoo K., Adachi W., Ishii J., Fujioka Y., Ohsumi Y., Inagaki F.
      Genes Cells 13:1211-1218(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS), INTERACTION WITH ATG19, MUTAGENESIS OF ARG-28; PRO-52 AND ARG-67.
    63. Cited for: STRUCTURE BY NMR OF MUTANT PRO-26, MUTAGENESIS OF LYS-26, CONJUGATION TO PHOSPHATIDYLETHANOLAMINE.
    64. "Solution structure of Atg8 reveals conformational polymorphism of the N-terminal domain."
      Schwarten M., Stoldt M., Mohrluder J., Willbold D.
      Biochem. Biophys. Res. Commun. 395:426-431(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    65. "Structural basis of Atg8 activation by a homodimeric E1, Atg7."
      Noda N.N., Satoo K., Fujioka Y., Kumeta H., Ogura K., Nakatogawa H., Ohsumi Y., Inagaki F.
      Mol. Cell 44:462-475(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR, X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH ATG7.
    66. "Insights into noncanonical E1 enzyme activation from the structure of autophagic E1 Atg7 with Atg8."
      Hong S.B., Kim B.W., Lee K.E., Kim S.W., Jeon H., Kim J., Song H.K.
      Nat. Struct. Mol. Biol. 18:1323-1330(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.91 ANGSTROMS) IN COMPLEX WITH ATG7.
    67. "Autophagy-related protein 32 acts as autophagic degron and directly initiates mitophagy."
      Kondo-Okamoto N., Noda N.N., Suzuki S.W., Nakatogawa H., Takahashi I., Matsunami M., Hashimoto A., Inagaki F., Ohsumi Y., Okamoto K.
      J. Biol. Chem. 287:10631-10638(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).

    Entry informationi

    Entry nameiATG8_YEAST
    AccessioniPrimary (citable) accession number: P38182
    Secondary accession number(s): D6VPS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2010 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3