Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P38182 (ATG8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Autophagy-related protein 8
Alternative name(s):
Autophagy-related ubiquitin-like modifier ATG8
Cytoplasm to vacuole targeting protein 5
Gene names
Name:ATG8
Synonyms:APG8, AUT7, CVT5
Ordered Locus Names:YBL078C
ORF Names:YBL0732
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length117 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ubiquitin-like modifier involved in cytoplasm to vacuole transport (Cvt) vesicles and autophagosomes formation. With ATG4, mediates the delivery of the vesicles and autophagosomes to the vacuole via the microtubule cytoskeleton. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Participates also in membrane fusion events that take place in the early secretory pathway. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy. Moreover not only conjugation, but also subsequent ATG8-PE deconjugation is an important step required to facilitate multiple events during macroautophagy, and especially for efficient autophagosome biogenesis, the assembly of Atg9-containing tubulovesicular clusters into phagophores/autophagosomes, and for the disassembly of PAS-associated ATG components. Plays also a role in regulation of filamentous growth. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.15 Ref.28 Ref.29 Ref.30 Ref.31 Ref.32 Ref.38 Ref.40 Ref.47 Ref.50 Ref.52 Ref.56

Subunit structure

Conjugation to phosphatidylethanolamine (PE) leads to homodimerization. Interacts with ATG1, ATG3, ATG4, ATG7, ATG12, ATG32, ATG34 and the C-terminal 10 residues domain of ATG19. Interacts also with the endoplasmic reticulum to Golgi v-SNARE protein BET1 and the vacuolar v-SNARE protein NYV1. Interacts with the UBX domain-containing protein SHP1. Ref.7 Ref.10 Ref.12 Ref.14 Ref.16 Ref.17 Ref.22 Ref.28 Ref.32 Ref.40 Ref.41 Ref.42 Ref.45 Ref.47 Ref.54 Ref.57 Ref.60 Ref.62

Subcellular location

Cytoplasmic vesiclecvt vesicle membrane; Lipid-anchor. Cytoplasmic vesicleautophagosome membrane; Lipid-anchor. Vacuole membrane; Lipid-anchor. Note: Membrane-associated through a lipid anchor. This association needs the 2 ubiquitin-like systems required for cytoplasm to vacuole transport and autophagy. ATG18 and ATG21 facilitate the recruitment of ATG8-PE to the site of autophagosome formation and protect it from premature cleavage by ATG4. Localizes to both the isolation membrane (IM) and the vacuole-isolation membrane contact site (VICS) during IM expansion. The IM is a membrane sac generated from the pre-autophagosomal structure that ultimately expands to become a mature autophagosome. Upon starvation, is also recruited to into unique membrane structures near SEC13-containing ER exit sites which lack components of the Golgi apparatus and the endosomes, and which were called a compartments for unconventional protein secretion (CUPS). Ref.7 Ref.9 Ref.10 Ref.11 Ref.13 Ref.15 Ref.17 Ref.20 Ref.23 Ref.24 Ref.25 Ref.26 Ref.27 Ref.28 Ref.33 Ref.35 Ref.37 Ref.39 Ref.44 Ref.46 Ref.48 Ref.49 Ref.50 Ref.61

Induction

Up-regulated upon starvation conditions. Expression is under the control of UME6 which acts along with a histone deacetylase complex including SIN3 and RPD3 to regulate negatively ATG8 levels and subsequent autophagic activity. Ref.43 Ref.58

Post-translational modification

The C-terminal Arg-117 residue of ATG8 is removed by ATG4 to expose Gly-116 at the C-terminus. This Gly-116 forms then a thioester bond with the 'Cys-507' of ATG7 (E1-like activating enzyme) before being transferred to the 'Cys-234' of ATG3 (the specific E2 conjugating enzyme), in order to be finally amidated with phosphatidylethanolamine. This lipid modification anchors ATG8 to membranes and can be reversed by ATG4, releasing soluble ATG8 (Ref.12).

Miscellaneous

Present with 2010 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ATG8 family.

Ontologies

Keywords
   Biological processAutophagy
Protein transport
Transport
Ubl conjugation pathway
   Cellular componentCytoplasmic vesicle
Membrane
Vacuole
   PTMLipoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processCVT pathway

Inferred from mutant phenotype Ref.7. Source: SGD

ER to Golgi vesicle-mediated transport

Inferred from mutant phenotype Ref.10. Source: SGD

autophagic vacuole assembly

Inferred from mutant phenotype Ref.15. Source: UniProtKB

autophagy

Inferred from mutant phenotype Ref.7. Source: UniProtKB

cellular protein complex localization

Inferred from mutant phenotype Ref.54. Source: SGD

late nucleophagy

Inferred from mutant phenotype Ref.56. Source: SGD

membrane fusion

Inferred from direct assay Ref.32. Source: SGD

mitochondrion degradation

Inferred from mutant phenotype PubMed 19793921. Source: SGD

piecemeal microautophagy of nucleus

Inferred from mutant phenotype PubMed 18701704. Source: SGD

protein targeting to vacuole involved in autophagy

Inferred from mutant phenotype Ref.54. Source: SGD

   Cellular_componentCvt vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

autophagic vacuole

Inferred from direct assay Ref.15. Source: UniProtKB

autophagic vacuole membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasm

Inferred from direct assay Ref.7. Source: UniProtKB

cytosol

Inferred from direct assay Ref.15. Source: UniProtKB

extrinsic component of membrane

Inferred from direct assay Ref.10. Source: SGD

fungal-type vacuole membrane

Inferred from direct assay Ref.10. Source: SGD

pre-autophagosomal structure

Inferred from direct assay Ref.13Ref.26Ref.28PubMed 23129774. Source: SGD

   Molecular_functionprotein tag

Inferred from mutant phenotype Ref.53. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ATG1P531043EBI-2684,EBI-2657
ATG3P403443EBI-2684,EBI-3381
ATG4P538673EBI-2684,EBI-29160
ATG7P388623EBI-2684,EBI-2677
BET1P228042EBI-2684,EBI-3550
NBR1Q145967EBI-2684,EBI-742698From a different organism.
SQSTM1Q135013EBI-2684,EBI-307104From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 116116Autophagy-related protein 8
PRO_0000017242
Propeptide1171Removed in mature form
PRO_0000017243

Sites

Site116 – 1172Cleavage; by ATG4

Amino acid modifications

Lipidation1161Phosphatidylethanolamine amidated glycine Ref.12

Experimental info

Mutagenesis261K → P: No effect on autophagic activity. Ref.63
Mutagenesis281R → D: Impairs ATG19-binding and autophagic activity. Ref.40 Ref.62
Mutagenesis481K → E: Impairs autophagic activity. Ref.40
Mutagenesis491Y → A: Impairs ATG19-binding, PE-conjugation, and autophagic activity. Ref.28 Ref.40
Mutagenesis501L → A: Impairs ATG19-binding and autophagic activity. Ref.28 Ref.40
Mutagenesis521P → A: Significantly decreases ATG19-binding and autophagic activity. Ref.62
Mutagenesis551L → A: Impairs autophagic activity. Ref.40
Mutagenesis671R → A: Significantly decreases ATG19-binding and autophagic activity. Ref.62
Mutagenesis771F → A: Impairs interaction with ATG4, cleavage by ATG4, PAS localization, and autophagic activity; when associated with A-79. Ref.28
Mutagenesis791F → A: Impairs interaction with ATG4, cleavage by ATG4, PAS localization, and autophagic activity; when associated with A-77. Ref.28
Mutagenesis1161G → A: No cleaving of the C-terminal R-117 by ATG4. Defect in Cvt pathway and autophagy. Ref.11
Mutagenesis1171R → M: Normal processing after G-116 by ATG4. Ref.11

Secondary structure

.................... 117
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P38182 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 1246CEFF5A038819

FASTA11713,627
        10         20         30         40         50         60 
MKSTFKSEYP FEKRKAESER IADRFKNRIP VICEKAEKSD IPEIDKRKYL VPADLTVGQF 

        70         80         90        100        110 
VYVIRKRIML PPEKAIFIFV NDTLPPTAAL MSAIYQEHKD KDGFLYVTYS GENTFGR 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae."
Tsukada M., Ohsumi Y.
FEBS Lett. 333:169-174(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway."
Harding T.M., Morano K.A., Scott S.V., Klionsky D.J.
J. Cell Biol. 131:591-602(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Aut2p and Aut7p, two novel microtubule-associated proteins are essential for delivery of autophagic vesicles to the vacuole."
Lang T., Schaeffeler E., Bernreuther D., Bredschneider M., Wolf D.H., Thumm M.
EMBO J. 17:3597-3607(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ATG4, SUBCELLULAR LOCATION.
[8]"Formation process of autophagosome is traced with Apg8/Aut7p in yeast."
Kirisako T., Baba M., Ishihara N., Miyazawa K., Ohsumi M., Yoshimori T., Noda T., Ohsumi Y.
J. Cell Biol. 147:435-446(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"The itinerary of a vesicle component, Aut7p/Cvt5p, terminates in the yeast vacuole via the autophagy/Cvt pathways."
Huang W.-P., Scott S.V., Kim J., Klionsky D.J.
J. Biol. Chem. 275:5845-5851(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]"Aut7p, a soluble autophagic factor, participates in multiple membrane trafficking processes."
Legesse-Miller A., Sagiv Y., Glozman R., Elazar Z.
J. Biol. Chem. 275:32966-32973(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH BET1 AND NYV1.
[11]"The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathway."
Kirisako T., Ichimura Y., Okada H., Kabeya Y., Mizushima N., Yoshimori T., Ohsumi M., Takao T., Noda T., Ohsumi Y.
J. Cell Biol. 151:263-276(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CLEAVAGE BY ATG4, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-116 AND ARG-117.
[12]"A ubiquitin-like system mediates protein lipidation."
Ichimura Y., Kirisako T., Takao T., Satomi Y., Shimonishi Y., Ishihara N., Mizushima N., Tanida I., Kominami E., Ohsumi M., Noda T., Ohsumi Y.
Nature 408:488-492(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ATG3 AND ATG7, LIPIDATION.
[13]"The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation."
Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y.
EMBO J. 20:5971-5981(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[14]"The C-terminal region of an Apg7p/Cvt2p is required for homodimerization and is essential for its E1 activity and E1-E2 complex formation."
Komatsu M., Tanida I., Ueno T., Ohsumi M., Ohsumi Y., Kominami E.
J. Biol. Chem. 276:9846-9854(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG7.
[15]"Membrane recruitment of Aut7p in the autophagy and cytoplasm to vacuole targeting pathways requires Aut1p, Aut2p, and the autophagy conjugation complex."
Kim J., Huang W.-P., Klionsky D.J.
J. Cell Biol. 152:51-64(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CLEAVAGE BY ATG4, SUBCELLULAR LOCATION.
[16]"Mechanism of cargo selection in the cytoplasm to vacuole targeting pathway."
Shintani T., Huang W.-P., Stromhaug P.E., Klionsky D.J.
Dev. Cell 3:825-837(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG19.
[17]"Convergence of multiple autophagy and cytoplasm to vacuole targeting components to a perivacuolar membrane compartment prior to de novo vesicle formation."
Kim J., Huang W.-P., Stromhaug P.E., Klionsky D.J.
J. Biol. Chem. 277:763-773(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ATG12 AND ATG19.
[18]"A unified nomenclature for yeast autophagy-related genes."
Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y.
Dev. Cell 5:539-545(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
[19]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[20]"Atg21 is required for effective recruitment of Atg8 to the preautophagosomal structure during the Cvt pathway."
Meiling-Wesse K., Barth H., Voss C., Eskelinen E.-L., Epple U.D., Thumm M.
J. Biol. Chem. 279:37741-37750(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: CONJUGATION TO PHOSPHATIDYLETHANOLAMINE, SUBCELLULAR LOCATION.
[21]"In vivo and in vitro reconstitution of atg8 conjugation essential for autophagy."
Ichimura Y., Imamura Y., Emoto K., Umeda M., Noda T., Ohsumi Y.
J. Biol. Chem. 279:40584-40592(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: CONJUGATION TO PHOSPHATIDYLETHANOLAMINE.
[22]"Autophagy-related protein 8 (Atg8) family interacting motif in Atg3 mediates the Atg3-Atg8 interaction and is crucial for the cytoplasm-to-vacuole targeting pathway."
Yamaguchi M., Noda N.N., Nakatogawa H., Kumeta H., Ohsumi Y., Inagaki F.
J. Biol. Chem. 285:29599-29607(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG3, CONJUGATION TO PHOSPHATIDYLETHANOLAMINE.
[23]"Atg21 is a phosphoinositide binding protein required for efficient lipidation and localization of Atg8 during uptake of aminopeptidase I by selective autophagy."
Stromhaug P.E., Reggiori F., Guan J., Wang C.-W., Klionsky D.J.
Mol. Biol. Cell 15:3553-3566(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[24]"Interrelationships among Atg proteins during autophagy in Saccharomyces cerevisiae."
Suzuki K., Noda T., Ohsumi Y.
Yeast 21:1057-1065(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[25]"Trs85 (Gsg1), a component of the TRAPP complexes, is required for the organization of the preautophagosomal structure during selective autophagy via the Cvt pathway."
Meiling-Wesse K., Epple U.D., Krick R., Barth H., Appelles A., Voss C., Eskelinen E.L., Thumm M.
J. Biol. Chem. 280:33669-33678(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[26]"Atg11 links cargo to the vesicle-forming machinery in the cytoplasm to vacuole targeting pathway."
Yorimitsu T., Klionsky D.J.
Mol. Biol. Cell 16:1593-1605(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[27]"The actin cytoskeleton is required for selective types of autophagy, but not nonspecific autophagy, in the yeast Saccharomyces cerevisiae."
Reggiori F., Monastyrska I., Shintani T., Klionsky D.J.
Mol. Biol. Cell 16:5843-5856(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[28]"Two newly identified sites in the ubiquitin-like protein Atg8 are essential for autophagy."
Amar N., Lustig G., Ichimura Y., Ohsumi Y., Elazar Z.
EMBO Rep. 7:635-642(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATG4, CLEAVAGE BY ATG4, MUTAGENESIS OF TYR-49; LEU-50; PHE-77 AND PHE-79.
[29]"Autophagy counterbalances endoplasmic reticulum expansion during the unfolded protein response."
Bernales S., McDonald K.L., Walter P.
PLoS Biol. 4:E423-E423(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[30]"The role of autophagy in mitochondria maintenance: characterization of mitochondrial functions in autophagy-deficient S. cerevisiae strains."
Zhang Y., Qi H., Taylor R., Xu W., Liu L.F., Jin S.
Autophagy 3:337-346(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[31]"Overexpression of autophagy-related genes inhibits yeast filamentous growth."
Ma J., Jin R., Dobry C.J., Lawson S.K., Kumar A.
Autophagy 3:604-609(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[32]"Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion."
Nakatogawa H., Ichimura Y., Ohsumi Y.
Cell 130:165-178(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: CONJUGATION TO PHOSPHATIDYLETHANOLAMINE, FUNCTION OF THE ATG8-PE CONJUGATE, SUBUNIT.
[33]"Hierarchy of Atg proteins in pre-autophagosomal structure organization."
Suzuki K., Kubota Y., Sekito T., Ohsumi Y.
Genes Cells 12:209-218(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[34]"Protein kinase A and Sch9 cooperatively regulate induction of autophagy in Saccharomyces cerevisiae."
Yorimitsu T., Zaman S., Broach J.R., Klionsky D.J.
Mol. Biol. Cell 18:4180-4189(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: CONJUGATION TO PHOSPHATIDYLETHANOLAMINE.
[35]"Quantitative analysis of autophagy-related protein stoichiometry by fluorescence microscopy."
Geng J., Baba M., Nair U., Klionsky D.J.
J. Cell Biol. 182:129-140(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[36]"In vivo reconstitution of autophagy in Saccharomyces cerevisiae."
Cao Y., Cheong H., Song H., Klionsky D.J.
J. Cell Biol. 182:703-713(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: CONJUGATION TO PHOSPHATIDYLETHANOLAMINE.
[37]"The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae."
Cheong H., Nair U., Geng J., Klionsky D.J.
Mol. Biol. Cell 19:668-681(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[38]"Atg8 controls phagophore expansion during autophagosome formation."
Xie Z., Nair U., Klionsky D.J.
Mol. Biol. Cell 19:3290-3298(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[39]"Indirect estimation of the area density of Atg8 on the phagophore."
Xie Z., Nair U., Geng J., Szefler M.B., Rothman E.D., Klionsky D.J.
Autophagy 5:217-220(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[40]"Mutation at the cargo-receptor binding site of Atg8 also affects its general autophagy regulation function."
Ho K.H., Chang H.E., Huang W.P.
Autophagy 5:461-471(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ATG19, MUTAGENESIS OF ARG-28; LYS-48; TYR-49; LEU-50 AND LEU-55.
[41]"Mitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy."
Okamoto K., Kondo-Okamoto N., Ohsumi Y.
Dev. Cell 17:87-97(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG32.
[42]"The amino-terminal region of Atg3 is essential for association with phosphatidylethanolamine in Atg8 lipidation."
Hanada T., Satomi Y., Takao T., Ohsumi Y.
FEBS Lett. 583:1078-1083(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG3, CONJUGATION TO PHOSPHATIDYLETHANOLAMINE.
[43]"Accumulation of non-superoxide anion reactive oxygen species mediates nitrogen-limited alcoholic fermentation by Saccharomyces cerevisiae."
Mendes-Ferreira A., Sampaio-Marques B., Barbosa C., Rodrigues F., Costa V., Mendes-Faia A., Ludovico P., Leao C.
Appl. Environ. Microbiol. 76:7918-7924(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[44]"Roles of the lipid-binding motifs of Atg18 and Atg21 in the cytoplasm to vacuole targeting pathway and autophagy."
Nair U., Cao Y., Xie Z., Klionsky D.J.
J. Biol. Chem. 285:11476-11488(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[45]"Selective transport of alpha-mannosidase by autophagic pathways: identification of a novel receptor, Atg34p."
Suzuki K., Kondo C., Morimoto M., Ohsumi Y.
J. Biol. Chem. 285:30019-30025(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG34.
[46]"The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy."
Yen W.L., Shintani T., Nair U., Cao Y., Richardson B.C., Li Z., Hughson F.M., Baba M., Klionsky D.J.
J. Cell Biol. 188:101-114(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[47]"Cdc48/p97 and Shp1/p47 regulate autophagosome biogenesis in concert with ubiquitin-like Atg8."
Krick R., Bremer S., Welter E., Schlotterhose P., Muehe Y., Eskelinen E.L., Thumm M.
J. Cell Biol. 190:965-973(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SHP1.
[48]"An Atg9-containing compartment that functions in the early steps of autophagosome biogenesis."
Mari M., Griffith J., Rieter E., Krishnappa L., Klionsky D.J., Reggiori F.
J. Cell Biol. 190:1005-1022(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[49]"Biogenesis of a novel compartment for autophagosome-mediated unconventional protein secretion."
Bruns C., McCaffery J.M., Curwin A.J., Duran J.M., Malhotra V.
J. Cell Biol. 195:979-992(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[50]"Mitophagy in yeast is independent of mitochondrial fission and requires the stress response gene WHI2."
Mendl N., Occhipinti A., Muller M., Wild P., Dikic I., Reichert A.S.
J. Cell Sci. 124:1339-1350(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[51]"Atg4 recycles inappropriately lipidated Atg8 to promote autophagosome biogenesis."
Nakatogawa H., Ishii J., Asai E., Ohsumi Y.
Autophagy 8:177-186(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ATG8-PE DECONJUGATION.
[52]"A role for Atg8-PE deconjugation in autophagosome biogenesis."
Nair U., Yen W.L., Mari M., Cao Y., Xie Z., Baba M., Reggiori F., Klionsky D.J.
Autophagy 8:780-793(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF ATG8-PE DECONJUGATION.
[53]"Dual roles of Atg8-PE deconjugation by Atg4 in autophagy."
Yu Z.Q., Ni T., Hong B., Wang H.Y., Jiang F.J., Zou S., Chen Y., Zheng X.L., Klionsky D.J., Liang Y., Xie Z.
Autophagy 8:883-892(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ATG8-PE DECONJUGATION.
[54]"Binding of the Atg1/ULK1 kinase to the ubiquitin-like protein Atg8 regulates autophagy."
Kraft C., Kijanska M., Kalie E., Siergiejuk E., Lee S.S., Semplicio G., Stoffel I., Brezovich A., Verma M., Hansmann I., Ammerer G., Hofmann K., Tooze S., Peter M.
EMBO J. 31:3691-3703(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG1.
[55]"Mechanism and functions of membrane binding by the Atg5-Atg12/Atg16 complex during autophagosome formation."
Romanov J., Walczak M., Ibiricu I., Schuchner S., Ogris E., Kraft C., Martens S.
EMBO J. 31:4304-4317(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: CONJUGATION TO PHOSPHATIDYLETHANOLAMINE.
[56]"A late form of nucleophagy in Saccharomyces cerevisiae."
Mijaljica D., Prescott M., Devenish R.J.
PLoS ONE 7:E40013-E40013(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[57]"The autophagy-related protein kinase Atg1 interacts with the ubiquitin-like protein Atg8 via the Atg8 family interacting motif to facilitate autophagosome formation."
Nakatogawa H., Ohbayashi S., Sakoh-Nakatogawa M., Kakuta S., Suzuki S.W., Kirisako H., Kondo-Kakuta C., Noda N.N., Yamamoto H., Ohsumi Y.
J. Biol. Chem. 287:28503-28507(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG1.
[58]"Ume6 transcription factor is part of a signaling cascade that regulates autophagy."
Bartholomew C.R., Suzuki T., Du Z., Backues S.K., Jin M., Lynch-Day M.A., Umekawa M., Kamath A., Zhao M., Xie Z., Inoki K., Klionsky D.J.
Proc. Natl. Acad. Sci. U.S.A. 109:11206-11210(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[59]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[60]"Function and molecular mechanism of acetylation in autophagy regulation."
Yi C., Ma M., Ran L., Zheng J., Tong J., Zhu J., Ma C., Sun Y., Zhang S., Feng W., Zhu L., Le Y., Gong X., Yan X., Hong B., Jiang F.J., Xie Z., Miao D., Deng H., Yu L.
Science 336:474-477(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG3, CONJUGATION TO PHOSPHATIDYLETHANOLAMINE.
[61]"Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae."
Suzuki K., Akioka M., Kondo-Kakuta C., Yamamoto H., Ohsumi Y.
J. Cell Sci. 126:2534-2544(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[62]"Structural basis of target recognition by Atg8/LC3 during selective autophagy."
Noda N.N., Kumeta H., Nakatogawa H., Satoo K., Adachi W., Ishii J., Fujioka Y., Ohsumi Y., Inagaki F.
Genes Cells 13:1211-1218(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS), INTERACTION WITH ATG19, MUTAGENESIS OF ARG-28; PRO-52 AND ARG-67.
[63]"The NMR structure of the autophagy-related protein Atg8."
Kumeta H., Watanabe M., Nakatogawa H., Yamaguchi M., Ogura K., Adachi W., Fujioka Y., Noda N.N., Ohsumi Y., Inagaki F.
J. Biomol. NMR 47:237-241(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF MUTANT PRO-26, MUTAGENESIS OF LYS-26, CONJUGATION TO PHOSPHATIDYLETHANOLAMINE.
[64]"Solution structure of Atg8 reveals conformational polymorphism of the N-terminal domain."
Schwarten M., Stoldt M., Mohrluder J., Willbold D.
Biochem. Biophys. Res. Commun. 395:426-431(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
[65]"Structural basis of Atg8 activation by a homodimeric E1, Atg7."
Noda N.N., Satoo K., Fujioka Y., Kumeta H., Ogura K., Nakatogawa H., Ohsumi Y., Inagaki F.
Mol. Cell 44:462-475(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR, X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH ATG7.
[66]"Insights into noncanonical E1 enzyme activation from the structure of autophagic E1 Atg7 with Atg8."
Hong S.B., Kim B.W., Lee K.E., Kim S.W., Jeon H., Kim J., Song H.K.
Nat. Struct. Mol. Biol. 18:1323-1330(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.91 ANGSTROMS) IN COMPLEX WITH ATG7.
[67]"Autophagy-related protein 32 acts as autophagic degron and directly initiates mitophagy."
Kondo-Okamoto N., Noda N.N., Suzuki S.W., Nakatogawa H., Takahashi I., Matsunami M., Hashimoto A., Inagaki F., Ohsumi Y., Okamoto K.
J. Biol. Chem. 287:10631-10638(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79489 Genomic DNA. Translation: CAA56032.1.
Z35839 Genomic DNA. Translation: CAA84899.1.
AY692870 Genomic DNA. Translation: AAT92889.1.
BK006936 Genomic DNA. Translation: DAA07045.1.
PIRS45432.
RefSeqNP_009475.1. NM_001178318.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2KQ7NMR-A1-117[»]
2KWCNMR-A1-116[»]
2LI5NMR-A1-116[»]
2ZPNX-ray2.70A/B/C/D1-116[»]
3RUIX-ray1.91B1-116[»]
3VH3X-ray2.00B1-116[»]
3VH4X-ray2.65B1-116[»]
3VXWX-ray3.00A1-116[»]
ProteinModelPortalP38182.
SMRP38182. Positions 4-116.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32625. 116 interactions.
DIPDIP-1197N.
IntActP38182. 15 interactions.
MINTMINT-391771.
STRING4932.YBL078C.

Proteomic databases

PaxDbP38182.
PeptideAtlasP38182.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL078C; YBL078C; YBL078C.
GeneID852200.
KEGGsce:YBL078C.

Organism-specific databases

CYGDYBL078c.
SGDS000000174. ATG8.

Phylogenomic databases

eggNOGNOG249730.
GeneTreeENSGT00390000012937.
HOGENOMHOG000232034.
KOK08341.
OMAIRRRITM.
OrthoDBEOG7712KX.

Enzyme and pathway databases

BioCycYEAST:G3O-28969-MONOMER.

Gene expression databases

GenevestigatorP38182.

Family and domain databases

InterProIPR004241. Atg8_like.
[Graphical view]
PANTHERPTHR10969. PTHR10969. 1 hit.
PfamPF02991. Atg8. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP38182.
NextBio970694.
PMAP-CutDBP38182.
PROP38182.

Entry information

Entry nameATG8_YEAST
AccessionPrimary (citable) accession number: P38182
Secondary accession number(s): D6VPS5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 16, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references