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Protein

Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase

Gene

ALG3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc(2)-PP-Dol. Sensitive to H.mrakii HM-1 killer toxin.2 Publications

Catalytic activityi

Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • alpha-1,3-mannosyltransferase activity Source: SGD
  • dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity Source: SGD

GO - Biological processi

  • oligosaccharide-lipid intermediate biosynthetic process Source: SGD
  • protein glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YBL082C-MONOMER.
YEAST:YBL082C-MONOMER.
BRENDAi2.4.1.258. 984.
ReactomeiR-SCE-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT58. Glycosyltransferase Family 58.

Names & Taxonomyi

Protein namesi
Recommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase (EC:2.4.1.258)
Alternative name(s):
Asparagine-linked glycosylation protein 3
Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase
HM-1 killer toxin resistance protein
Gene namesi
Name:ALG3
Synonyms:RHK1
Ordered Locus Names:YBL082C
ORF Names:YBL0720
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL082C.
SGDiS000000178. ALG3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 41LumenalSequence analysisAdd BLAST41
Transmembranei42 – 62HelicalSequence analysisAdd BLAST21
Topological domaini63 – 95CytoplasmicSequence analysisAdd BLAST33
Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
Topological domaini117 – 137LumenalSequence analysisAdd BLAST21
Transmembranei138 – 158HelicalSequence analysisAdd BLAST21
Topological domaini159 – 171CytoplasmicSequence analysisAdd BLAST13
Transmembranei172 – 192HelicalSequence analysisAdd BLAST21
Topological domaini193 – 216LumenalSequence analysisAdd BLAST24
Transmembranei217 – 237HelicalSequence analysisAdd BLAST21
Topological domaini238 – 242CytoplasmicSequence analysis5
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 302LumenalSequence analysisAdd BLAST39
Transmembranei303 – 323HelicalSequence analysisAdd BLAST21
Topological domaini324 – 345CytoplasmicSequence analysisAdd BLAST22
Transmembranei346 – 366HelicalSequence analysisAdd BLAST21
Topological domaini367 – 381LumenalSequence analysisAdd BLAST15
Transmembranei382 – 402HelicalSequence analysisAdd BLAST21
Topological domaini403 – 415CytoplasmicSequence analysisAdd BLAST13
Transmembranei416 – 436HelicalSequence analysisAdd BLAST21
Topological domaini437 – 458LumenalSequence analysisAdd BLAST22

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi275A → V in ALG3-1; leads to an underglycosylation of secretory proteins. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805651 – 458Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferaseAdd BLAST458

Proteomic databases

MaxQBiP38179.
PRIDEiP38179.

Interactioni

Protein-protein interaction databases

BioGridi32621. 86 interactors.
DIPiDIP-4854N.
MINTiMINT-489474.

Structurei

3D structure databases

ProteinModelPortaliP38179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 58 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000013904.
HOGENOMiHOG000237555.
InParanoidiP38179.
KOiK03845.
OMAiRKFLYEW.
OrthoDBiEOG092C26UZ.

Family and domain databases

InterProiIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
PANTHERiPTHR12646. PTHR12646. 1 hit.
PfamiPF05208. ALG3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38179-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGEQSPQGE KSLQRKQFVR PPLDLWQDLK DGVRYVIFDC RANLIVMPLL
60 70 80 90 100
ILFESMLCKI IIKKVAYTEI DYKAYMEQIE MIQLDGMLDY SQVSGGTGPL
110 120 130 140 150
VYPAGHVLIY KMMYWLTEGM DHVERGQVFF RYLYLLTLAL QMACYYLLHL
160 170 180 190 200
PPWCVVLACL SKRLHSIYVL RLFNDCFTTL FMVVTVLGAI VASRCHQRPK
210 220 230 240 250
LKKSLALVIS ATYSMAVSIK MNALLYFPAM MISLFILNDA NVILTLLDLV
260 270 280 290 300
AMIAWQVAVA VPFLRSFPQQ YLHCAFNFGR KFMYQWSINW QMMDEEAFND
310 320 330 340 350
KRFHLALLIS HLIALTTLFV TRYPRILPDL WSSLCHPLRK NAVLNANPAK
360 370 380 390 400
TIPFVLIASN FIGVLFSRSL HYQFLSWYHW TLPILIFWSG MPFFVGPIWY
410 420 430 440 450
VLHEWCWNSY PPNSQASTLL LALNTVLLLL LALTQLSGSV ALAKSHLRTT

SSMEKKLN
Length:458
Mass (Da):52,861
Last modified:October 1, 1994 - v1
Checksum:i1C2FEAD6D459C249
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86G → R in AAA75352 (PubMed:7754714).Curated1
Sequence conflicti187 – 198LGAIV…RCHQR → FRGYRGQQVPSA in AAA75352 (PubMed:7754714).CuratedAdd BLAST12
Sequence conflicti266S → Q in AAA75352 (PubMed:7754714).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79489 Genomic DNA. Translation: CAA56024.1.
Z35844 Genomic DNA. Translation: CAA84904.1.
M89908 Genomic DNA. Translation: AAA75352.1.
BK006936 Genomic DNA. Translation: DAA07041.1.
PIRiS45424.
RefSeqiNP_009471.1. NM_001178322.1.

Genome annotation databases

EnsemblFungiiYBL082C; YBL082C; YBL082C.
GeneIDi852196.
KEGGisce:YBL082C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79489 Genomic DNA. Translation: CAA56024.1.
Z35844 Genomic DNA. Translation: CAA84904.1.
M89908 Genomic DNA. Translation: AAA75352.1.
BK006936 Genomic DNA. Translation: DAA07041.1.
PIRiS45424.
RefSeqiNP_009471.1. NM_001178322.1.

3D structure databases

ProteinModelPortaliP38179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32621. 86 interactors.
DIPiDIP-4854N.
MINTiMINT-489474.

Protein family/group databases

CAZyiGT58. Glycosyltransferase Family 58.

Proteomic databases

MaxQBiP38179.
PRIDEiP38179.

Protocols and materials databases

DNASUi852196.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL082C; YBL082C; YBL082C.
GeneIDi852196.
KEGGisce:YBL082C.

Organism-specific databases

EuPathDBiFungiDB:YBL082C.
SGDiS000000178. ALG3.

Phylogenomic databases

GeneTreeiENSGT00390000013904.
HOGENOMiHOG000237555.
InParanoidiP38179.
KOiK03845.
OMAiRKFLYEW.
OrthoDBiEOG092C26UZ.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:YBL082C-MONOMER.
YEAST:YBL082C-MONOMER.
BRENDAi2.4.1.258. 984.
ReactomeiR-SCE-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiP38179.

Family and domain databases

InterProiIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
PANTHERiPTHR12646. PTHR12646. 1 hit.
PfamiPF05208. ALG3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG3_YEAST
AccessioniPrimary (citable) accession number: P38179
Secondary accession number(s): D6VPS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.