ID   AVT5_YEAST              Reviewed;         459 AA.
AC   P38176; D6VPR5;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 2.
DT   27-MAR-2024, entry version 148.
DE   RecName: Full=Vacuolar amino acid transporter 5;
GN   Name=AVT5; OrderedLocusNames=YBL089W; ORFNames=YBL0703;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7502586; DOI=10.1002/yea.320111112;
RA   Obermaier B., Gassenhuber J., Piravandi E., Domdey H.;
RT   "Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces
RT   cerevisiae chromosome II.";
RL   Yeast 11:1103-1112(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA   Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA   Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA   Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA   Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA   Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA   Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA   Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA   Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA   Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA   Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA   Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA   Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA   Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA   Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA   Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA   Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA   Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA   Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA   Mewes H.-W., Kleine K.;
RT   "Complete DNA sequence of yeast chromosome II.";
RL   EMBO J. 13:5795-5809(1994).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=11274162; DOI=10.1074/jbc.m008028200;
RA   Russnak R., Konczal D., McIntire S.L.;
RT   "A family of yeast proteins mediating bidirectional vacuolar amino acid
RT   transport.";
RL   J. Biol. Chem. 276:23849-23857(2001).
RN   [5]
RP   IDENTIFICATION OF PROBABLE INITIATION SITE.
RX   PubMed=12748633; DOI=10.1038/nature01644;
RA   Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT   "Sequencing and comparison of yeast species to identify genes and
RT   regulatory elements.";
RL   Nature 423:241-254(2003).
CC   -!- FUNCTION: Probable amino acid transporter of unknown specificity.
CC       {ECO:0000269|PubMed:11274162}.
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:11274162};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:11274162}.
CC   -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC       {ECO:0000305}.
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DR   EMBL; X79489; CAA56013.1; -; Genomic_DNA.
DR   EMBL; Z35850; CAA84910.1; -; Genomic_DNA.
DR   EMBL; BK006936; DAA07035.1; -; Genomic_DNA.
DR   PIR; S45413; S45413.
DR   RefSeq; NP_009464.2; NM_001178329.1.
DR   AlphaFoldDB; P38176; -.
DR   BioGRID; 32615; 191.
DR   DIP; DIP-2846N; -.
DR   IntAct; P38176; 4.
DR   MINT; P38176; -.
DR   STRING; 4932.YBL089W; -.
DR   TCDB; 2.A.18.6.10; the amino acid/auxin permease (aaap) family.
DR   PaxDb; 4932-YBL089W; -.
DR   PeptideAtlas; P38176; -.
DR   EnsemblFungi; YBL089W_mRNA; YBL089W; YBL089W.
DR   GeneID; 852189; -.
DR   KEGG; sce:YBL089W; -.
DR   AGR; SGD:S000000185; -.
DR   SGD; S000000185; AVT5.
DR   VEuPathDB; FungiDB:YBL089W; -.
DR   eggNOG; KOG1305; Eukaryota.
DR   GeneTree; ENSGT00940000170090; -.
DR   HOGENOM; CLU_009020_1_1_1; -.
DR   InParanoid; P38176; -.
DR   OMA; SHYADMD; -.
DR   OrthoDB; 5485318at2759; -.
DR   BioCyc; YEAST:G3O-28978-MONOMER; -.
DR   BioGRID-ORCS; 852189; 6 hits in 10 CRISPR screens.
DR   PRO; PR:P38176; -.
DR   Proteomes; UP000002311; Chromosome II.
DR   RNAct; P38176; Protein.
DR   GO; GO:0000329; C:fungal-type vacuole membrane; IBA:GO_Central.
DR   GO; GO:0016020; C:membrane; ISS:SGD.
DR   GO; GO:0061459; F:L-arginine transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0005313; F:L-glutamate transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0005290; F:L-histidine transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015189; F:L-lysine transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015194; F:L-serine transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0005302; F:L-tyrosine transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0022857; F:transmembrane transporter activity; ISS:SGD.
DR   GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR   GO; GO:0055085; P:transmembrane transport; ISS:SGD.
DR   InterPro; IPR013057; AA_transpt_TM.
DR   PANTHER; PTHR22950; AMINO ACID TRANSPORTER; 1.
DR   PANTHER; PTHR22950:SF646; SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTER 10-RELATED; 1.
DR   Pfam; PF01490; Aa_trans; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport; Vacuole.
FT   CHAIN           1..459
FT                   /note="Vacuolar amino acid transporter 5"
FT                   /id="PRO_0000093838"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        33..53
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        82..102
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        131..151
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        161..181
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        206..226
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        240..260
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        278..298
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        364..384
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        386..406
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        434..454
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          335..354
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        335..353
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   459 AA;  50892 MW;  8D3D533A63CD190D CRC64;
     MPSNVRSGVL TLLHTACGAG VLAMPFAFKP FGLMPGLITL TFCGICSLCG LLLQTRIAKY
     VPKSENASFA KLTQLINPSI SVVFDFAIAV KCFGVGVSYL IIVGDLVPQI VQSIFYRNDD
     NMSGSQEHHM FLDRRLYITL IIVFVISPLC FKRSLNSLRY ASMIAIVSVA YLSGLIIYHF
     VNRHQLERGQ VYFMVPHGDS QSHSPLTTLP IFVFAYTCHH NMFSVINEQV DKSFKVIRRI
     PIFAIVLAYF LYIIIGGTGY MTFGENIVGN ILTLYPNSIS TTIGRLAMLL LVMLAFPLQC
     HPCRSSVKNI IIFIENFRKG KLYDNRASFI PLDNFNSEDP QEAPTQQNNE EPNLRSESLR
     HINIITLCIL LFSYLLAISI TSLAKVLAIV GATGSTSISF ILPGLFGYKL IGSEFTGTNE
     RVPTSIKIFK YLSLSLFIWG IAVMVASLSA IVFLGTSSH
//