P38175 (RT21_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
37S ribosomal protein MRP21, mitochondrial

Gene names

Name:	MRP21
Synonyms:	MRP50
Ordered Locus Names:	YBL090W
ORF Names:	YBL0702

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the mitochondrial small ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.5
Subcellular location	Mitochondrion Ref.7 Ref.9.
Miscellaneous	Present with 1270 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the ribosomal protein S21P family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial translational initiation Inferred from genetic interaction Ref.5. Source: SGD
Cellular component	mitochondrial small ribosomal subunit Inferred from direct assay Ref.5. Source: SGD
Molecular function	structural constituent of ribosome Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 17	17	Mitochondrion Potential
	Chain	18 – 177	160	37S ribosomal protein MRP21, mitochondrial		PRO_0000202444
Experimental info
	Mutagenesis	115	1	E → K in MRP21-1; suppresses translation defects of mutations in the 5'-untranslated leaders of mitochondrial mRNAs. Ref.5
	Mutagenesis	121	1	N → K in MRP21-3; suppresses translation defects of mutations in the 5'-untranslated leaders of mitochondrial mRNAs. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P38175 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: EE6676C74827F189	FASTA	177	20,395
	10 20 30 40 50 60 MLKSTLRLSR ISLRRGFTTI DCLRQQNSDI DKIILNPIKL AQGSNSDRGQ TSKSKTDNAD 70 80 90 100 110 120 ILSMEIPVDM MQSAGRINKR ELLSEAEIAR SSVENAQMRF NSGKSIIVNK NNPAESFKRL 130 140 150 160 170 NRIMFENNIP GDKRSQRFYM KPGKVAELKR SQRHRKEFMM GFKRLIEIVK DAKRKGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II." Obermaier B., Gassenhuber J., Piravandi E., Domdey H. Yeast 11:1103-1112(1995) [PubMed: 7502586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Functional interactions between yeast mitochondrial ribosomes and mRNA 5' untranslated leaders." Green-Willms N.S., Fox T.D., Costanzo M.C. Mol. Cell. Biol. 18:1826-1834(1998) [PubMed: 9528754] [Abstract] Cited for: SUBUNIT, MUTAGENESIS OF GLU-115 AND ASN-121.
[6]	"Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components." Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K. Eur. J. Biochem. 269:5203-5214(2002) [PubMed: 12392552] [Abstract] Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL SMALL COMPLEX, MASS SPECTROMETRY.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X79489 Genomic DNA. Translation: CAA56012.1. Z35851 Genomic DNA. Translation: CAA84911.1. AY558290 Genomic DNA. Translation: AAS56616.1. BK006936 Genomic DNA. Translation: DAA07034.1.
PIR	S45412.
RefSeq	NP_009463.1. NM_001178330.1.
3D structure databases
ProteinModelPortal	P38175.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6704N.
IntAct	P38175. 4 interactions.
MINT	MINT-633266.
STRING	P38175.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL090W; YBL090W; YBL090W.
GeneID	852188.
KEGG	sce:YBL090W.
NMPDR	fig|4932.3.peg.149.
Organism-specific databases
CYGD	YBL090w.
SGD	S000000186. MRP21.
Phylogenomic databases
eggNOG	fuNOG11889.
GeneTree	EFGT00050000008620.
OrthoDB	EOG4J9R8D.
Gene expression databases
ArrayExpress	P38175.
Genevestigator	P38175.
GermOnline	YBL090W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001911. Ribosomal_S21. [Graphical view]
Pfam	PF01165. Ribosomal_S21. 1 hit. [Graphical view]
PROSITE	PS01181. RIBOSOMAL_S21. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	970661.

Entry information

Entry name

RT21_YEAST

Accession

Primary (citable) accession number: P38175
Secondary accession number(s): D6VPR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 14, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program