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P38170

- CND2_YEAST

UniProt

P38170 - CND2_YEAST

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Protein

Condensin complex subunit 2

Gene

BRN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex probably also plays a role during interphase.2 Publications

GO - Molecular functioni

  1. chromatin binding Source: SGD

GO - Biological processi

  1. DNA unwinding involved in DNA replication Source: SGD
  2. mitotic chromosome condensation Source: SGD
  3. mitotic sister chromatid segregation Source: SGD
  4. tRNA gene clustering Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA condensation, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-28983-MONOMER.
ReactomeiREACT_188908. Condensation of Prometaphase Chromosomes.

Names & Taxonomyi

Protein namesi
Recommended name:
Condensin complex subunit 2
Alternative name(s):
Barren homolog
CAPH homolog
Gene namesi
Name:BRN1
Ordered Locus Names:YBL097W
ORF Names:YBL0830
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBL097w.
SGDiS000000193. BRN1.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication. Chromosome 1 Publication
Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, condensin associates with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nuclear condensin complex Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 754754Condensin complex subunit 2PRO_0000095047Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei245 – 2451Phosphoserine1 Publication
Modified residuei548 – 5481Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38170.
PaxDbiP38170.

Expressioni

Gene expression databases

GenevestigatoriP38170.

Interactioni

Subunit structurei

Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: BRN1, YCS4 and YCG1/YCS5.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SMC2P389892EBI-4792,EBI-17412

Protein-protein interaction databases

BioGridi32608. 37 interactions.
DIPiDIP-4662N.
IntActiP38170. 11 interactions.
MINTiMINT-539913.
STRINGi4932.YBL097W.

Family & Domainsi

Sequence similaritiesi

Belongs to the CND2 (condensin subunit 2) family.Curated

Phylogenomic databases

eggNOGiCOG5229.
GeneTreeiENSGT00390000004149.
HOGENOMiHOG000246707.
InParanoidiP38170.
KOiK06676.
OMAiPYPCHPP.
OrthoDBiEOG7NPG2Q.

Family and domain databases

InterProiIPR022816. Condensin_barren_su2.
[Graphical view]
PfamiPF05786. Cnd2. 1 hit.
[Graphical view]
PIRSFiPIRSF017126. Condensin_H. 1 hit.

Sequencei

Sequence statusi: Complete.

P38170-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTQLRYENN DDDERVEYNL FTNRSTMMAN FEEWIKMATD NKINSRNSWN
60 70 80 90 100
FALIDYFYDL DVLKDGENNI NFQKASATLD GCIKIYSSRV DSVTTETGKL
110 120 130 140 150
LSGLAQRKTN GASNGDDSNG GNGEGLGGDS DEANIEIDPL TGMPISNDPD
160 170 180 190 200
VNNTRRRVYN RVLETTLVEF ETIKMKELDQ ELIIDPLFKK ALVDFDEGGA
210 220 230 240 250
KSLLLNTLNI DNTARVIFDA SIKDTQNVGQ GKLQRKEEEL IERDSLVDDE
260 270 280 290 300
NEPSQSLIST RNDSTVNDSV ISAPSMEDEI LSLGMDFIKF DQIAVCEISG
310 320 330 340 350
SIEQLRNVVE DINQAKDFIE NVNNRFDNFL TEEELQAAVP DNAEDDSDGF
360 370 380 390 400
DMGMQQELCY PDENHDNTSH DEQDDDNVNS TTGSIFEKDL MAYFDENLNR
410 420 430 440 450
NWRGREHWKV RNFKKANLVN KESDLLEETR TTIGDTTDKN TTDDKSMDTK
460 470 480 490 500
KKHKQKKVLE IDFFKTDDSF EDKVFASKGR TKIDMPIKNR KNDTHYLLPD
510 520 530 540 550
DFHFSTDRIT RLFIKPGQKM SLFSHRKHTR GDVSSGLFEK STVSANHSNN
560 570 580 590 600
DIPTIADEHF WADNYERKEQ EEKEKEQSKE VGDVVGGALD NPFEDDMDGV
610 620 630 640 650
DFNQAFEGTD DNEEASVKLD LQDDEDHKFP IRENKVTYSR VSKKVDVRRL
660 670 680 690 700
KKNVWRSINN LIQEHDSRKN REQSSNDSET HTEDESTKEL KFSDIIQGIS
710 720 730 740 750
KMYSDDTLKD ISTSFCFICL LHLANEHGLQ ITHTENYNDL IVNYEDLATT

QAAS
Length:754
Mass (Da):86,216
Last modified:July 27, 2011 - v3
Checksum:i7B30373738D4AE7A
GO

Sequence cautioni

The sequence CAA56003.1 differs from that shown. Reason: Frameshift at position 25.
The sequence CAA84919.1 differs from that shown. Reason: Frameshift at position 25.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti517 – 5171G → A in CAA56003. (PubMed:7502586)Curated
Sequence conflicti517 – 5171G → A in CAA84919. (PubMed:7813418)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79489 Genomic DNA. Translation: CAA56003.1. Frameshift.
Z35858 Genomic DNA. Translation: CAA84919.1. Frameshift.
AY558077 Genomic DNA. Translation: AAS56403.1.
BK006936 Genomic DNA. Translation: DAA07028.2.
PIRiS45403.
RefSeqiNP_009455.3. NM_001178337.2.

Genome annotation databases

EnsemblFungiiYBL097W; YBL097W; YBL097W.
GeneIDi852180.
KEGGisce:YBL097W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79489 Genomic DNA. Translation: CAA56003.1 . Frameshift.
Z35858 Genomic DNA. Translation: CAA84919.1 . Frameshift.
AY558077 Genomic DNA. Translation: AAS56403.1 .
BK006936 Genomic DNA. Translation: DAA07028.2 .
PIRi S45403.
RefSeqi NP_009455.3. NM_001178337.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32608. 37 interactions.
DIPi DIP-4662N.
IntActi P38170. 11 interactions.
MINTi MINT-539913.
STRINGi 4932.YBL097W.

Proteomic databases

MaxQBi P38170.
PaxDbi P38170.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBL097W ; YBL097W ; YBL097W .
GeneIDi 852180.
KEGGi sce:YBL097W.

Organism-specific databases

CYGDi YBL097w.
SGDi S000000193. BRN1.

Phylogenomic databases

eggNOGi COG5229.
GeneTreei ENSGT00390000004149.
HOGENOMi HOG000246707.
InParanoidi P38170.
KOi K06676.
OMAi PYPCHPP.
OrthoDBi EOG7NPG2Q.

Enzyme and pathway databases

BioCyci YEAST:G3O-28983-MONOMER.
Reactomei REACT_188908. Condensation of Prometaphase Chromosomes.

Miscellaneous databases

NextBioi 970643.
PROi P38170.

Gene expression databases

Genevestigatori P38170.

Family and domain databases

InterProi IPR022816. Condensin_barren_su2.
[Graphical view ]
Pfami PF05786. Cnd2. 1 hit.
[Graphical view ]
PIRSFi PIRSF017126. Condensin_H. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
    Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
    Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1678.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 517.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-754.
    Strain: ATCC 204508 / S288c.
  5. "The condensin complex governs chromosome condensation and mitotic transmission of rDNA."
    Freeman L., Aragon-Alcaide L., Strunnikov A.V.
    J. Cell Biol. 149:811-824(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A CONDENSIN COMPLEX WITH SMC2; SMC4; YCG1 AND YCS4.
  6. "Mitotic chromosome condensation requires Brn1p, the yeast homologue of Barren."
    Lavoie B.D., Tuffo K.M., Oh S., Koshland D., Holm C.
    Mol. Biol. Cell 11:1293-1304(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Chromosome condensation factor Brn1p is required for chromatid separation in mitosis."
    Ouspenski I.I., Cabello O.A., Brinkley B.R.
    Mol. Biol. Cell 11:1305-1313(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SMC2.
  8. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF FRAMESHIFT.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245 AND SER-548, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCND2_YEAST
AccessioniPrimary (citable) accession number: P38170
Secondary accession number(s): D6VPQ8, Q6Q5G8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 704 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3