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P38170 (CND2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Condensin complex subunit 2
Alternative name(s):
Barren homolog
CAPH homolog
Gene names
Name:BRN1
Ordered Locus Names:YBL097W
ORF Names:YBL0830
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length754 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex probably also plays a role during interphase. Ref.6 Ref.7

Subunit structure

Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: BRN1, YCS4 and YCG1/YCS5. Ref.5

Subcellular location

Nucleus. Cytoplasm. Chromosome. Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, condensin associates with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase. Ref.7

Miscellaneous

Present with 704 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the CND2 (condensin subunit 2) family.

Sequence caution

The sequence CAA56003.1 differs from that shown. Reason: Frameshift at position 25.

The sequence CAA84919.1 differs from that shown. Reason: Frameshift at position 25.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SMC2P389892EBI-4792,EBI-17412

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 754754Condensin complex subunit 2
PRO_0000095047

Amino acid modifications

Modified residue2451Phosphoserine Ref.11
Modified residue5481Phosphoserine Ref.11

Experimental info

Sequence conflict5171G → A in CAA56003. Ref.1
Sequence conflict5171G → A in CAA84919. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P38170 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 7B30373738D4AE7A

FASTA75486,216
        10         20         30         40         50         60 
MTTQLRYENN DDDERVEYNL FTNRSTMMAN FEEWIKMATD NKINSRNSWN FALIDYFYDL 

        70         80         90        100        110        120 
DVLKDGENNI NFQKASATLD GCIKIYSSRV DSVTTETGKL LSGLAQRKTN GASNGDDSNG 

       130        140        150        160        170        180 
GNGEGLGGDS DEANIEIDPL TGMPISNDPD VNNTRRRVYN RVLETTLVEF ETIKMKELDQ 

       190        200        210        220        230        240 
ELIIDPLFKK ALVDFDEGGA KSLLLNTLNI DNTARVIFDA SIKDTQNVGQ GKLQRKEEEL 

       250        260        270        280        290        300 
IERDSLVDDE NEPSQSLIST RNDSTVNDSV ISAPSMEDEI LSLGMDFIKF DQIAVCEISG 

       310        320        330        340        350        360 
SIEQLRNVVE DINQAKDFIE NVNNRFDNFL TEEELQAAVP DNAEDDSDGF DMGMQQELCY 

       370        380        390        400        410        420 
PDENHDNTSH DEQDDDNVNS TTGSIFEKDL MAYFDENLNR NWRGREHWKV RNFKKANLVN 

       430        440        450        460        470        480 
KESDLLEETR TTIGDTTDKN TTDDKSMDTK KKHKQKKVLE IDFFKTDDSF EDKVFASKGR 

       490        500        510        520        530        540 
TKIDMPIKNR KNDTHYLLPD DFHFSTDRIT RLFIKPGQKM SLFSHRKHTR GDVSSGLFEK 

       550        560        570        580        590        600 
STVSANHSNN DIPTIADEHF WADNYERKEQ EEKEKEQSKE VGDVVGGALD NPFEDDMDGV 

       610        620        630        640        650        660 
DFNQAFEGTD DNEEASVKLD LQDDEDHKFP IRENKVTYSR VSKKVDVRRL KKNVWRSINN 

       670        680        690        700        710        720 
LIQEHDSRKN REQSSNDSET HTEDESTKEL KFSDIIQGIS KMYSDDTLKD ISTSFCFICL 

       730        740        750 
LHLANEHGLQ ITHTENYNDL IVNYEDLATT QAAS 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / FY1678.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 517.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-754.
Strain: ATCC 204508 / S288c.
[5]"The condensin complex governs chromosome condensation and mitotic transmission of rDNA."
Freeman L., Aragon-Alcaide L., Strunnikov A.V.
J. Cell Biol. 149:811-824(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A CONDENSIN COMPLEX WITH SMC2; SMC4; YCG1 AND YCS4.
[6]"Mitotic chromosome condensation requires Brn1p, the yeast homologue of Barren."
Lavoie B.D., Tuffo K.M., Oh S., Koshland D., Holm C.
Mol. Biol. Cell 11:1293-1304(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Chromosome condensation factor Brn1p is required for chromatid separation in mitosis."
Ouspenski I.I., Cabello O.A., Brinkley B.R.
Mol. Biol. Cell 11:1305-1313(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SMC2.
[8]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF FRAMESHIFT.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245 AND SER-548, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79489 Genomic DNA. Translation: CAA56003.1. Frameshift.
Z35858 Genomic DNA. Translation: CAA84919.1. Frameshift.
AY558077 Genomic DNA. Translation: AAS56403.1.
BK006936 Genomic DNA. Translation: DAA07028.2.
PIRS45403.
RefSeqNP_009455.3. NM_001178337.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32608. 37 interactions.
DIPDIP-4662N.
IntActP38170. 11 interactions.
MINTMINT-539913.
STRING4932.YBL097W.

Proteomic databases

MaxQBP38170.
PaxDbP38170.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL097W; YBL097W; YBL097W.
GeneID852180.
KEGGsce:YBL097W.

Organism-specific databases

CYGDYBL097w.
SGDS000000193. BRN1.

Phylogenomic databases

eggNOGCOG5229.
GeneTreeENSGT00390000004149.
HOGENOMHOG000246707.
KOK06676.
OMAPYPCHPP.
OrthoDBEOG7NPG2Q.

Enzyme and pathway databases

BioCycYEAST:G3O-28983-MONOMER.

Gene expression databases

GenevestigatorP38170.

Family and domain databases

InterProIPR022816. Condensin_barren_su2.
[Graphical view]
PfamPF05786. Cnd2. 1 hit.
[Graphical view]
PIRSFPIRSF017126. Condensin_H. 1 hit.
ProtoNetSearch...

Other

NextBio970643.
PROP38170.

Entry information

Entry nameCND2_YEAST
AccessionPrimary (citable) accession number: P38170
Secondary accession number(s): D6VPQ8, Q6Q5G8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 27, 2011
Last modified: June 11, 2014
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families