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Protein

Condensin complex subunit 2

Gene

BRN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex probably also plays a role during interphase.2 Publications

Miscellaneous

Present with 704 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • chromatin binding Source: SGD
  • DNA topoisomerase (ATP-hydrolyzing) activator activity Source: GO_Central
  • DNA topoisomerase binding Source: GO_Central

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic chromosome condensation Source: SGD
  • mitotic sister chromatid segregation Source: SGD
  • tRNA gene clustering Source: SGD

Keywordsi

Biological processCell cycle, Cell division, DNA condensation, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-28983-MONOMER
ReactomeiR-SCE-2514853 Condensation of Prometaphase Chromosomes

Names & Taxonomyi

Protein namesi
Recommended name:
Condensin complex subunit 2
Alternative name(s):
Barren homolog
CAPH homolog
Gene namesi
Name:BRN1
Ordered Locus Names:YBL097W
ORF Names:YBL0830
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL097W
SGDiS000000193 BRN1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000950471 – 754Condensin complex subunit 2Add BLAST754

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei245PhosphoserineCombined sources1
Modified residuei548PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38170
PaxDbiP38170
PRIDEiP38170

PTM databases

CarbonylDBiP38170
iPTMnetiP38170

Interactioni

Subunit structurei

Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: BRN1, YCS4 and YCG1/YCS5.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi32608, 422 interactors
DIPiDIP-4662N
IntActiP38170, 18 interactors
MINTiP38170
STRINGi4932.YBL097W

Structurei

Secondary structure

1754
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi385 – 398Combined sources14
Helixi399 – 401Combined sources3
Helixi405 – 408Combined sources4
Beta strandi463 – 465Combined sources3
Helixi469 – 474Combined sources6
Helixi487 – 490Combined sources4
Turni493 – 496Combined sources4
Helixi506 – 510Combined sources5
Beta strandi518 – 520Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5OQNX-ray3.15B384-529[»]
5OQOX-ray3.25B384-529[»]
5OQPX-ray2.98B384-529[»]
5OQQX-ray2.79C/D384-529[»]
SMRiP38170
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CND2 (condensin subunit 2) family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000004149
HOGENOMiHOG000246707
InParanoidiP38170
KOiK06676
OMAiEWIKMAT
OrthoDBiEOG092C0XD0

Family and domain databases

InterProiView protein in InterPro
IPR022816 Condensin_barren_su2
PANTHERiPTHR13108 PTHR13108, 1 hit
PfamiView protein in Pfam
PF05786 Cnd2, 1 hit
PIRSFiPIRSF017126 Condensin_H, 1 hit

Sequencei

Sequence statusi: Complete.

P38170-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTQLRYENN DDDERVEYNL FTNRSTMMAN FEEWIKMATD NKINSRNSWN
60 70 80 90 100
FALIDYFYDL DVLKDGENNI NFQKASATLD GCIKIYSSRV DSVTTETGKL
110 120 130 140 150
LSGLAQRKTN GASNGDDSNG GNGEGLGGDS DEANIEIDPL TGMPISNDPD
160 170 180 190 200
VNNTRRRVYN RVLETTLVEF ETIKMKELDQ ELIIDPLFKK ALVDFDEGGA
210 220 230 240 250
KSLLLNTLNI DNTARVIFDA SIKDTQNVGQ GKLQRKEEEL IERDSLVDDE
260 270 280 290 300
NEPSQSLIST RNDSTVNDSV ISAPSMEDEI LSLGMDFIKF DQIAVCEISG
310 320 330 340 350
SIEQLRNVVE DINQAKDFIE NVNNRFDNFL TEEELQAAVP DNAEDDSDGF
360 370 380 390 400
DMGMQQELCY PDENHDNTSH DEQDDDNVNS TTGSIFEKDL MAYFDENLNR
410 420 430 440 450
NWRGREHWKV RNFKKANLVN KESDLLEETR TTIGDTTDKN TTDDKSMDTK
460 470 480 490 500
KKHKQKKVLE IDFFKTDDSF EDKVFASKGR TKIDMPIKNR KNDTHYLLPD
510 520 530 540 550
DFHFSTDRIT RLFIKPGQKM SLFSHRKHTR GDVSSGLFEK STVSANHSNN
560 570 580 590 600
DIPTIADEHF WADNYERKEQ EEKEKEQSKE VGDVVGGALD NPFEDDMDGV
610 620 630 640 650
DFNQAFEGTD DNEEASVKLD LQDDEDHKFP IRENKVTYSR VSKKVDVRRL
660 670 680 690 700
KKNVWRSINN LIQEHDSRKN REQSSNDSET HTEDESTKEL KFSDIIQGIS
710 720 730 740 750
KMYSDDTLKD ISTSFCFICL LHLANEHGLQ ITHTENYNDL IVNYEDLATT

QAAS
Length:754
Mass (Da):86,216
Last modified:July 27, 2011 - v3
Checksum:i7B30373738D4AE7A
GO

Sequence cautioni

The sequence CAA56003 differs from that shown. Reason: Frameshift at position 25.Curated
The sequence CAA84919 differs from that shown. Reason: Frameshift at position 25.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti517G → A in CAA56003 (PubMed:7502586).Curated1
Sequence conflicti517G → A in CAA84919 (PubMed:7813418).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79489 Genomic DNA Translation: CAA56003.1 Frameshift.
Z35858 Genomic DNA Translation: CAA84919.1 Frameshift.
AY558077 Genomic DNA Translation: AAS56403.1
BK006936 Genomic DNA Translation: DAA07028.2
PIRiS45403
RefSeqiNP_009455.3, NM_001178337.2

Genome annotation databases

EnsemblFungiiYBL097W; YBL097W; YBL097W
GeneIDi852180
KEGGisce:YBL097W

Similar proteinsi

Entry informationi

Entry nameiCND2_YEAST
AccessioniPrimary (citable) accession number: P38170
Secondary accession number(s): D6VPQ8, Q6Q5G8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health