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P38170

- CND2_YEAST

UniProt

P38170 - CND2_YEAST

Protein

Condensin complex subunit 2

Gene

BRN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex probably also plays a role during interphase.2 Publications

    GO - Molecular functioni

    1. chromatin binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. DNA unwinding involved in DNA replication Source: SGD
    2. mitotic chromosome condensation Source: SGD
    3. mitotic sister chromatid segregation Source: SGD
    4. tRNA gene clustering Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, DNA condensation, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28983-MONOMER.
    ReactomeiREACT_188908. Condensation of Prometaphase Chromosomes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Condensin complex subunit 2
    Alternative name(s):
    Barren homolog
    CAPH homolog
    Gene namesi
    Name:BRN1
    Ordered Locus Names:YBL097W
    ORF Names:YBL0830
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBL097w.
    SGDiS000000193. BRN1.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication. Chromosome 1 Publication
    Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, condensin associates with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nuclear condensin complex Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Chromosome, Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 754754Condensin complex subunit 2PRO_0000095047Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei245 – 2451Phosphoserine1 Publication
    Modified residuei548 – 5481Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38170.
    PaxDbiP38170.

    Expressioni

    Gene expression databases

    GenevestigatoriP38170.

    Interactioni

    Subunit structurei

    Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: BRN1, YCS4 and YCG1/YCS5.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SMC2P389892EBI-4792,EBI-17412

    Protein-protein interaction databases

    BioGridi32608. 37 interactions.
    DIPiDIP-4662N.
    IntActiP38170. 11 interactions.
    MINTiMINT-539913.
    STRINGi4932.YBL097W.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CND2 (condensin subunit 2) family.Curated

    Phylogenomic databases

    eggNOGiCOG5229.
    GeneTreeiENSGT00390000004149.
    HOGENOMiHOG000246707.
    KOiK06676.
    OMAiPYPCHPP.
    OrthoDBiEOG7NPG2Q.

    Family and domain databases

    InterProiIPR022816. Condensin_barren_su2.
    [Graphical view]
    PfamiPF05786. Cnd2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017126. Condensin_H. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P38170-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTQLRYENN DDDERVEYNL FTNRSTMMAN FEEWIKMATD NKINSRNSWN    50
    FALIDYFYDL DVLKDGENNI NFQKASATLD GCIKIYSSRV DSVTTETGKL 100
    LSGLAQRKTN GASNGDDSNG GNGEGLGGDS DEANIEIDPL TGMPISNDPD 150
    VNNTRRRVYN RVLETTLVEF ETIKMKELDQ ELIIDPLFKK ALVDFDEGGA 200
    KSLLLNTLNI DNTARVIFDA SIKDTQNVGQ GKLQRKEEEL IERDSLVDDE 250
    NEPSQSLIST RNDSTVNDSV ISAPSMEDEI LSLGMDFIKF DQIAVCEISG 300
    SIEQLRNVVE DINQAKDFIE NVNNRFDNFL TEEELQAAVP DNAEDDSDGF 350
    DMGMQQELCY PDENHDNTSH DEQDDDNVNS TTGSIFEKDL MAYFDENLNR 400
    NWRGREHWKV RNFKKANLVN KESDLLEETR TTIGDTTDKN TTDDKSMDTK 450
    KKHKQKKVLE IDFFKTDDSF EDKVFASKGR TKIDMPIKNR KNDTHYLLPD 500
    DFHFSTDRIT RLFIKPGQKM SLFSHRKHTR GDVSSGLFEK STVSANHSNN 550
    DIPTIADEHF WADNYERKEQ EEKEKEQSKE VGDVVGGALD NPFEDDMDGV 600
    DFNQAFEGTD DNEEASVKLD LQDDEDHKFP IRENKVTYSR VSKKVDVRRL 650
    KKNVWRSINN LIQEHDSRKN REQSSNDSET HTEDESTKEL KFSDIIQGIS 700
    KMYSDDTLKD ISTSFCFICL LHLANEHGLQ ITHTENYNDL IVNYEDLATT 750
    QAAS 754
    Length:754
    Mass (Da):86,216
    Last modified:July 27, 2011 - v3
    Checksum:i7B30373738D4AE7A
    GO

    Sequence cautioni

    The sequence CAA56003.1 differs from that shown. Reason: Frameshift at position 25.
    The sequence CAA84919.1 differs from that shown. Reason: Frameshift at position 25.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti517 – 5171G → A in CAA56003. (PubMed:7502586)Curated
    Sequence conflicti517 – 5171G → A in CAA84919. (PubMed:7813418)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79489 Genomic DNA. Translation: CAA56003.1. Frameshift.
    Z35858 Genomic DNA. Translation: CAA84919.1. Frameshift.
    AY558077 Genomic DNA. Translation: AAS56403.1.
    BK006936 Genomic DNA. Translation: DAA07028.2.
    PIRiS45403.
    RefSeqiNP_009455.3. NM_001178337.2.

    Genome annotation databases

    EnsemblFungiiYBL097W; YBL097W; YBL097W.
    GeneIDi852180.
    KEGGisce:YBL097W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79489 Genomic DNA. Translation: CAA56003.1 . Frameshift.
    Z35858 Genomic DNA. Translation: CAA84919.1 . Frameshift.
    AY558077 Genomic DNA. Translation: AAS56403.1 .
    BK006936 Genomic DNA. Translation: DAA07028.2 .
    PIRi S45403.
    RefSeqi NP_009455.3. NM_001178337.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32608. 37 interactions.
    DIPi DIP-4662N.
    IntActi P38170. 11 interactions.
    MINTi MINT-539913.
    STRINGi 4932.YBL097W.

    Proteomic databases

    MaxQBi P38170.
    PaxDbi P38170.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBL097W ; YBL097W ; YBL097W .
    GeneIDi 852180.
    KEGGi sce:YBL097W.

    Organism-specific databases

    CYGDi YBL097w.
    SGDi S000000193. BRN1.

    Phylogenomic databases

    eggNOGi COG5229.
    GeneTreei ENSGT00390000004149.
    HOGENOMi HOG000246707.
    KOi K06676.
    OMAi PYPCHPP.
    OrthoDBi EOG7NPG2Q.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28983-MONOMER.
    Reactomei REACT_188908. Condensation of Prometaphase Chromosomes.

    Miscellaneous databases

    NextBioi 970643.
    PROi P38170.

    Gene expression databases

    Genevestigatori P38170.

    Family and domain databases

    InterProi IPR022816. Condensin_barren_su2.
    [Graphical view ]
    Pfami PF05786. Cnd2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017126. Condensin_H. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
      Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
      Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1678.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 517.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-754.
      Strain: ATCC 204508 / S288c.
    5. "The condensin complex governs chromosome condensation and mitotic transmission of rDNA."
      Freeman L., Aragon-Alcaide L., Strunnikov A.V.
      J. Cell Biol. 149:811-824(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A CONDENSIN COMPLEX WITH SMC2; SMC4; YCG1 AND YCS4.
    6. "Mitotic chromosome condensation requires Brn1p, the yeast homologue of Barren."
      Lavoie B.D., Tuffo K.M., Oh S., Koshland D., Holm C.
      Mol. Biol. Cell 11:1293-1304(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Chromosome condensation factor Brn1p is required for chromatid separation in mitosis."
      Ouspenski I.I., Cabello O.A., Brinkley B.R.
      Mol. Biol. Cell 11:1305-1313(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SMC2.
    8. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
      Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
      Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF FRAMESHIFT.
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245 AND SER-548, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCND2_YEAST
    AccessioniPrimary (citable) accession number: P38170
    Secondary accession number(s): D6VPQ8, Q6Q5G8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 115 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 704 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3