Reviewed, UniProtKB/Swiss-Prot P38167 (ECM21_YEAST)

Names and origin

Protein names

Recommended name:
    Protein ECM21
Alternative name(s):
    Extracellular mutant protein 21

Gene names

Name:	ECM21
Ordered Locus Names:	YBL101C
ORF Names:	YBL0814

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1117 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in cell wall organization and biogenesis.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 799 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the CSR2 family.
Sequence caution	The sequence CAA55995.1 differs from that shown. Reason: Frameshift at positions 8, 964 and 967. The sequence CAB39760.1 differs from that shown. Reason: Frameshift at positions 8, 964 and 967.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cytoplasm
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall organization Ref.4 Inferred from mutant phenotype. Source: SGD ubiquitin-dependent endocytosis Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Ref.5 Inferred from direct assay. Source: SGD
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct ubiquitin protein ligase binding Inferred from physical interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-21359,EBI-21359
RSP5	P39940	1	EBI-21359,EBI-16219

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1117	1117	Protein ECM21		PRO_0000086917
Regions
	Compositional bias	490 – 511	22	Ser-rich
Amino acid modifications
	Modified residue	13	1	Phosphoserine Ref.10
	Modified residue	16	1	Phosphoserine Ref.11
	Modified residue	18	1	Phosphoserine Ref.10 Ref.11
	Modified residue	56	1	Phosphoserine Ref.3
	Modified residue	87	1	Phosphothreonine Ref.3
	Modified residue	123	1	Phosphothreonine Ref.3
	Modified residue	126	1	Phosphoserine Ref.3
	Modified residue	127	1	Phosphoserine Ref.3
	Modified residue	140	1	Phosphoserine Ref.10 Ref.11 Ref.8 Ref.9
	Modified residue	182	1	Phosphoserine Ref.11
	Modified residue	286	1	Phosphoserine Ref.11
	Modified residue	300	1	Phosphoserine Ref.10
	Modified residue	306	1	Phosphoserine Ref.10
	Modified residue	308	1	Phosphoserine Ref.10
	Modified residue	527	1	Phosphoserine Ref.10 Ref.11 Ref.9
	Modified residue	550	1	Phosphoserine Ref.11 Ref.3
	Modified residue	775	1	Phosphoserine Ref.3 Ref.9
	Modified residue	962	1	Phosphoserine Ref.3
	Modified residue	965	1	Phosphoserine Ref.11
	Modified residue	968	1	Phosphoserine Ref.11
	Modified residue	1028	1	Phosphothreonine Ref.11
	Modified residue	1030	1	Phosphoserine Ref.11 Ref.3
	Modified residue	1035	1	Phosphoserine Ref.10 Ref.11 Ref.3
	Modified residue	1038	1	Phosphoserine Ref.10 Ref.11
	Modified residue	1039	1	Phosphoserine Ref.11 Ref.3
	Modified residue	1041	1	Phosphothreonine Ref.10
	Cross-link	577		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3 Ref.7
	Cross-link	651		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3
	Cross-link	712		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3
	Cross-link	794		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3
	Cross-link	807		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3
	Cross-link	1024		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3
Experimental info
	Sequence conflict	964 – 967	4	PSGY → HPDT Ref.1
	Sequence conflict	964 – 967	4	PSGY → HPDT Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P38167-1 [UniParc]. Last modified March 1, 2005. Version 2. Checksum: 7DE365C1625B8BA9	FASTA	1,117	123,610
	10 20 30 40 50 60 MPFITSRPVA KNSSHSLSET DLNQSKGQPF QPSPTKKLGS MQQRRRSSTI RHALSSLLGG 70 80 90 100 110 120 ANVHSPAVLN NTTKGGNNNG NIRSSNTDAQ LLGKKQNKQP PPNARRHSTT AIQGSISDSA 130 140 150 160 170 180 TTTPRSSTSD TNRRTSGRLS VDQEPRISGG RYSQIEEDST VLDFDDDHNS SAVVSSDLSS 190 200 210 220 230 240 TSLTRLANSK KFNEQFLIEY LTARGLLGPK TVLSNEYLKI SISTSGESVF LPTISSNDDE 250 260 270 280 290 300 YLSRLNGLND GTDDAEADFF MDGIDQQEGN TPSLATTAAA TESGGSINEN RDTLLRENNS 310 320 330 340 350 360 GDHPGSGSEL NTRSVEIDSS MVSYSIAVIV SVKKPTRFTD MQLELCSRVK VFWNTGVPPT 370 380 390 400 410 420 KTFNEEFYNA ASMKWNLNDE NFDLFVPLSI SPDDQMIENN SNDRQMRLFK NIPTEERLYL 430 440 450 460 470 480 DKTKTKASLL NAIDVNKTHL YQPGDYVFLV PVVFSNHIPE TIYLPSARVS YRLRLATKAI 490 500 510 520 530 540 NRKGFYRQDS NSPQPIVSPD SSSSLSSTTS SLKLTETESA QAHRRISNTL FSKVKNHLHM 550 560 570 580 590 600 SSHQLKNEES GEEDIFAEYP IKVIRTPPPV AVSTANKPIY INRVWTDSLS YEISFAQKYV 610 620 630 640 650 660 SLNSEVPIKI KLAPICKNVC VKRIHVSITE RVTFVSKGYE YEYDQTDPVA KDPYNPYYLD 670 680 690 700 710 720 FASKRRKERS VSLFEIRTKE KGTRALREEI VENSFNDNLL SYSPFDDDSD SKGNPKERLG 730 740 750 760 770 780 ITEPIIIETK LKFPKYEDLD KRTAKIIPPY GIDAYTSIPN PEHAVANGPS HRRPSVIGFL 790 800 810 820 830 840 SGHKGSKSHE ENEKPVYDPK FHQTIIKSNS GLPVKTHTRL NTPKRGLYLD SLHFSNVYCR 850 860 870 880 890 900 HKLEIMLRIS KPDPECPSKL RHYEVLIDTP IFLVSEQCNS GNMELPTYDM ATMEGKGNQV 910 920 930 940 950 960 PLSMNSDFFG NTCPPPPTFE EAISVPASPI VSPMGSPNIM ASYDPDLLSI QQLNLSRTTS 970 980 990 1000 1010 1020 VSGPSGYSDD AGVPNVNRNS ISNANAMNGS ISNSAFVSGN SGQGVARARA TSVNDRSRFN 1030 1040 1050 1060 1070 1080 NLDKLLSTPS PVNRSHNSSP TNGLSQANGT VRIPNATTEN SKDKQNEFFK KGYTLANVKD 1090 1100 1110 DEEQEGIVSS SSADSLLSHG NEPPRYDEIV PLMSDEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II." Obermaier B., Gassenhuber J., Piravandi E., Domdey H. Yeast 11:1103-1112(1995) [PubMed: 7502586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, MASS SPECTROMETRY, UBIQUITINATION AT LYS-577; LYS-651; LYS-712; LYS-794; LYS-807 AND LYS-1024, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56; THR-87; THR-123; SER-126; SER-127; SER-550; SER-775; SER-962; SER-1030; SER-1035 AND SER-1039.
[4]	"Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae." Lussier M., White A.-M., Sheraton J., di Paolo T., Treadwell J., Southard S.B., Horenstein C.I., Chen-Weiner J., Ram A.F.J., Kapteyn J.C., Roemer T.W., Vo D.H., Bondoc D.C., Hall J., Zhong W.-W., Sdicu A.-M., Davies J., Klis F.M., Robbins P.W., Bussey H. Genetics 147:435-450(1997) [PubMed: 9335584] [Abstract] Cited for: IDENTIFICATION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery." Hitchcock A.L., Auld K., Gygi S.P., Silver P.A. Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed: 14557538] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-577, MASS SPECTROMETRY.
[8]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140, MASS SPECTROMETRY.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140; SER-527 AND SER-775, MASS SPECTROMETRY.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13; SER-18; SER-140; SER-300; SER-306; SER-308; SER-527; SER-1035; SER-1038 AND THR-1041, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16; SER-18; SER-140; SER-182; SER-286; SER-527; SER-550; SER-965; SER-968; THR-1028; SER-1030; SER-1035; SER-1038 AND SER-1039, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X79489 Genomic DNA. Translation: CAA55995.1. Frameshift. Z35862 Genomic DNA. Translation: CAB39760.1. Frameshift.
PIR	S45395.
RefSeq	NP_009449.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:791N.
IntAct	P38167. 5 interactions.
STRING	P38167.
Genome annotation databases
Ensembl	YBL101C; YBL101C; YBL101C; Saccharomyces cerevisiae. [Genome view]
GeneID	852173.
KEGG	sce:YBL101C.
NMPDR	fig|4932.3.peg.134.
Organism-specific databases
CYGD	YBL101c.
SGD	S000000197. ECM21.
Phylogenomic databases
HOGENOM	P38167.
OMA	SSNDDEY
OrthoDB	EOG9QFXXM
Gene expression databases
ArrayExpress	P38167.
Genevestigator	P38167.
GermOnline	YBL101C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	970626.

Entry information

Entry name

ECM21_YEAST

Accession

Primary (citable) accession number: P38167

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	March 1, 2005
Last modified:	November 24, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)